This document outlines Baraka Gitari's Masters thesis proposal on bacterial cellulose. The goal is to synthesize, process, and characterize bacterial cellulose. Bacterial cellulose is produced intracellularly by bacteria like Gluconacetobacter xylinus using glucose as a substrate. It has properties like being biodegradable, sustainable, and able to be used for applications like capacitors or reinforced plastics. The plan is to grow the bacteria using a modified Hestrin-Schramm medium, extract the cellulose, electrospin it, and characterize it using various techniques. Challenges include maintaining aseptic conditions during growth and synthesis.
Nanocomposite shows considerable applications in different fields because of larger surface area, and greater aspect ratio, with fascinating properties. Being environmentally friendly, applications of nanocomposites offer new technology and business opportunities for several sectors, such as aerospace, automotive, electronics, and biotechnology industries.
characteristics exploration of n ii cuzn nano-composite coated permanent magnetsIJEAB
This paper presents the synthesis of compound using Citrate Precursor Sol- Gel Method and Ball millingfor grinding the compound. X-ray diffraction measurements (XRD) confirmed the formation of single-phase cubic spinel structure. The average crystallite size was calculated using XRD pattern and confirmed by Scanning Electron Microscope (SEM). The electromagnetic properties were investigated using Vector Network Analyzer (VNA) and molar magnetic susceptibility measurements. The magnetic measurements have proved that the entire preparation method has considerable effect in enhancing the magnetic properties of the system. And an application of PMBLDC machine design with ferrite coated permanent magnets having competitive power density and efficiency. The influence of temperature variation on the magnets on the electric machine performance is also observed.
Copper indium sulphide films were deposited by the
pulse plating technique with different OFF times in the range of
5s – 30s and at a constant current density of 5 mA cm-2. The
films exhibited single phase copper indium sulphide. The grain
size increased with decrease of OFF time. Optical band gap of the
films increased from 1.44– 1.497 eV with decrease of OFF time.
Optical constants (refractive index, n, and extinction co-efficient,
k) of the films have been obtained in the wavelength range 800 -
1700 nm by using spectrophotometric measurement. The
obtained results concerning the absorption index yield the energy
gap in addition to the type of the allowed optical transitions.
N/m* ratio has been obtained from refractive index data. The
dispersion of refractive index is analyzed by using a single
oscillator model.
An Experimental Analysis to Determine Ultimate Tensile Strength of Jute Reinf...IJSRD
From past few decades, there is been substantial growth and development in field of Composites. Advanced materials and Composites are being used in almost every industry in some form or the other. Composites have found wider applicability and liking in designing industries. This has triggered researchers towards this emerging technology. Jute reinforced composites may be used in combination with biodegradable polymer or to replace conventional glass fibre reinforced composites. In this case, the main concern is their impact resistance. The production of hybrid laminates by coupling layers of glass fibre reinforced with jute reinforced laminates, proved also effective to improve the mechanical characteristics. In recent years, a number of studies have been carried out, aimed to compare properties of jute fibre reinforced laminates. This project studies Mechanical properties of Jute Reinforced Glass Fibre Composite by Acousto-Ultrasonic Technique. The specimens were tested for UTS on UTM and the correlation factors were determined by comparing SWF (Stress Wave Factors) and UTS. The comparative analyses are presented in results.
brief introduction on bone structure and common bone disease, mechanism of targeting, structure and composition of bone delivery system with mentioning some gaps in current research
Nanocomposite shows considerable applications in different fields because of larger surface area, and greater aspect ratio, with fascinating properties. Being environmentally friendly, applications of nanocomposites offer new technology and business opportunities for several sectors, such as aerospace, automotive, electronics, and biotechnology industries.
characteristics exploration of n ii cuzn nano-composite coated permanent magnetsIJEAB
This paper presents the synthesis of compound using Citrate Precursor Sol- Gel Method and Ball millingfor grinding the compound. X-ray diffraction measurements (XRD) confirmed the formation of single-phase cubic spinel structure. The average crystallite size was calculated using XRD pattern and confirmed by Scanning Electron Microscope (SEM). The electromagnetic properties were investigated using Vector Network Analyzer (VNA) and molar magnetic susceptibility measurements. The magnetic measurements have proved that the entire preparation method has considerable effect in enhancing the magnetic properties of the system. And an application of PMBLDC machine design with ferrite coated permanent magnets having competitive power density and efficiency. The influence of temperature variation on the magnets on the electric machine performance is also observed.
Copper indium sulphide films were deposited by the
pulse plating technique with different OFF times in the range of
5s – 30s and at a constant current density of 5 mA cm-2. The
films exhibited single phase copper indium sulphide. The grain
size increased with decrease of OFF time. Optical band gap of the
films increased from 1.44– 1.497 eV with decrease of OFF time.
Optical constants (refractive index, n, and extinction co-efficient,
k) of the films have been obtained in the wavelength range 800 -
1700 nm by using spectrophotometric measurement. The
obtained results concerning the absorption index yield the energy
gap in addition to the type of the allowed optical transitions.
N/m* ratio has been obtained from refractive index data. The
dispersion of refractive index is analyzed by using a single
oscillator model.
An Experimental Analysis to Determine Ultimate Tensile Strength of Jute Reinf...IJSRD
From past few decades, there is been substantial growth and development in field of Composites. Advanced materials and Composites are being used in almost every industry in some form or the other. Composites have found wider applicability and liking in designing industries. This has triggered researchers towards this emerging technology. Jute reinforced composites may be used in combination with biodegradable polymer or to replace conventional glass fibre reinforced composites. In this case, the main concern is their impact resistance. The production of hybrid laminates by coupling layers of glass fibre reinforced with jute reinforced laminates, proved also effective to improve the mechanical characteristics. In recent years, a number of studies have been carried out, aimed to compare properties of jute fibre reinforced laminates. This project studies Mechanical properties of Jute Reinforced Glass Fibre Composite by Acousto-Ultrasonic Technique. The specimens were tested for UTS on UTM and the correlation factors were determined by comparing SWF (Stress Wave Factors) and UTS. The comparative analyses are presented in results.
brief introduction on bone structure and common bone disease, mechanism of targeting, structure and composition of bone delivery system with mentioning some gaps in current research
EFFECT OF ULTRAVIOLET RADIATION ON STRUCTURAL PROPERTIES OF NANOWIRESijoejournal
Copper nanowires were prepared through electrochemical template synthesis using Nucleopore polycarbonate membranes having nominal pore sizes of 800nm and 15nm diameter. The 800nm and 15nm nanowires thus grown were viewed under SEM and TEM respectively, while their FCC crystallographic structure was confirmed through X-ray and electron diffraction patterns. The X-ray diffraction peaks indicated strong texturing for (200). The texturing was found to reduce significantly upon exposure to ultraviolet radiation.
Chitosan capped Silver nanoparticles used as Pressure sensorsIOSR Journals
In the present work, we report the synthesis and characterization of silver nanoparticles, capped with chitosan (biopolymer ). The majority of the particles produced in this way had sizes around 18 nm. Composite films of capped silver nanoparticles and chitosan polymer were studied to understand the charge transport under different pressure. Films of different compositions were prepared to measure current voltage curves across the film thickness. The results reveal that these materials exhibit electrical conductivity as predicted by the “classical theory of percolation”. Pressure dependent electrical conductivity and these composites can be explored to develop low cost pressure sensors.
In the present work micromachining of PMMA was carried out using KrF excimer laser. Excimer laser pulse with a wavelength of 248 nm was generated with a coherent COMPexPro 110 excimer laser system.
A micro-hole of Ø150 μm was machined on PMMA substrate during the experimentation. The PMMA substrate was mounted on the translation stage. The PMMA substrates were ex-posed to different number of pulses (1, 2, 5, 10, 20, 50 and 100) at repetition rate of 2, 5 and 10 Hz respectively by keeping the pulse energy unchanged at 200 mJ. In the present experimentation, the effect of pulse repetition rate and
number of pulses on ablation depth has been investigated. The experimental results for micromachining
demonstrate ablation process as a photo-chemical mechanism. The results of the experimentation have
revealed that, ablation depth is directly proportional to pulse number & pulse repetition rate has no significant effects on the ablation depth.
Characterization of Chemical and Physical Properties of Palm Fibers msejjournal
Natural fibers like palm fibers provides new hope for researchers to compete with hazardous synthetic
fibers with its excellent chemical and physical properties This work investigates the extraction of various
fibers that are available from various portions of the palm tree and to characterize its chemical and
physical properties. Also the results were compared with other natural fibers.
Influence of Biofield Treatment on Physical and Structural Characteristics of...albertdivis
Barium oxide (BaO) and zinc sulfide (ZnS) are well known for their applications in electrical, optical and chemical industries. The present study was aimed to evaluate the impact of biofield treatment on the structural and physical properties of BaO and ZnS powder.
Biofield impact on Atomic and Physical Characteristics of MagnesiumMahendra Kumar Trivedi
The presentation highlights the impact of biofield treatment on atomic and physical properties of magnesium powder. The magnesium samples were characterized using XRD analysis, surface area analysis and Particle Size analysis to evaluate the impact of Mahendra Trivedi's unique energy treatment on Atomic and Physical properties of Magnesium.
The International Journal of Engineering and Science (The IJES)theijes
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
polysaccharide consisting of a linear chain of several hundred to many thousands of β(1→4) linked D-glucose units.
Cellulose is an important structural component of the primary cell wall of green plants, many forms of algae and the oomycetes. Some species of bacteria secrete it to form biofilms.
Cellulose is the most abundant organic polymer on Earth. The cellulose content of cotton fiber is 90%, that of wood is 40–50% and that of dried hemp is approximately 45%.
Cellulose is mainly used to produce paperboard and paper.
Smaller quantities are converted into a wide variety of derivative products such as cellophane and rayon.
Conversion of cellulose from energy crops into biofuels such as cellulosic ethanol is under investigation as an alternative fuel source.
Cellulose for industrial use is mainly obtained from wood pulp and cotton.
Some animals, particularly ruminants and termites, can digest cellulose with the help of symbiotic micro-organisms that live in their guts, such as Trichonympha.
In humans, cellulose acts as a hydrophilic bulking agent for feces and is often referred to as a "dietary fiber".
EFFECT OF ULTRAVIOLET RADIATION ON STRUCTURAL PROPERTIES OF NANOWIRESijoejournal
Copper nanowires were prepared through electrochemical template synthesis using Nucleopore polycarbonate membranes having nominal pore sizes of 800nm and 15nm diameter. The 800nm and 15nm nanowires thus grown were viewed under SEM and TEM respectively, while their FCC crystallographic structure was confirmed through X-ray and electron diffraction patterns. The X-ray diffraction peaks indicated strong texturing for (200). The texturing was found to reduce significantly upon exposure to ultraviolet radiation.
Chitosan capped Silver nanoparticles used as Pressure sensorsIOSR Journals
In the present work, we report the synthesis and characterization of silver nanoparticles, capped with chitosan (biopolymer ). The majority of the particles produced in this way had sizes around 18 nm. Composite films of capped silver nanoparticles and chitosan polymer were studied to understand the charge transport under different pressure. Films of different compositions were prepared to measure current voltage curves across the film thickness. The results reveal that these materials exhibit electrical conductivity as predicted by the “classical theory of percolation”. Pressure dependent electrical conductivity and these composites can be explored to develop low cost pressure sensors.
In the present work micromachining of PMMA was carried out using KrF excimer laser. Excimer laser pulse with a wavelength of 248 nm was generated with a coherent COMPexPro 110 excimer laser system.
A micro-hole of Ø150 μm was machined on PMMA substrate during the experimentation. The PMMA substrate was mounted on the translation stage. The PMMA substrates were ex-posed to different number of pulses (1, 2, 5, 10, 20, 50 and 100) at repetition rate of 2, 5 and 10 Hz respectively by keeping the pulse energy unchanged at 200 mJ. In the present experimentation, the effect of pulse repetition rate and
number of pulses on ablation depth has been investigated. The experimental results for micromachining
demonstrate ablation process as a photo-chemical mechanism. The results of the experimentation have
revealed that, ablation depth is directly proportional to pulse number & pulse repetition rate has no significant effects on the ablation depth.
Characterization of Chemical and Physical Properties of Palm Fibers msejjournal
Natural fibers like palm fibers provides new hope for researchers to compete with hazardous synthetic
fibers with its excellent chemical and physical properties This work investigates the extraction of various
fibers that are available from various portions of the palm tree and to characterize its chemical and
physical properties. Also the results were compared with other natural fibers.
Influence of Biofield Treatment on Physical and Structural Characteristics of...albertdivis
Barium oxide (BaO) and zinc sulfide (ZnS) are well known for their applications in electrical, optical and chemical industries. The present study was aimed to evaluate the impact of biofield treatment on the structural and physical properties of BaO and ZnS powder.
Biofield impact on Atomic and Physical Characteristics of MagnesiumMahendra Kumar Trivedi
The presentation highlights the impact of biofield treatment on atomic and physical properties of magnesium powder. The magnesium samples were characterized using XRD analysis, surface area analysis and Particle Size analysis to evaluate the impact of Mahendra Trivedi's unique energy treatment on Atomic and Physical properties of Magnesium.
The International Journal of Engineering and Science (The IJES)theijes
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
polysaccharide consisting of a linear chain of several hundred to many thousands of β(1→4) linked D-glucose units.
Cellulose is an important structural component of the primary cell wall of green plants, many forms of algae and the oomycetes. Some species of bacteria secrete it to form biofilms.
Cellulose is the most abundant organic polymer on Earth. The cellulose content of cotton fiber is 90%, that of wood is 40–50% and that of dried hemp is approximately 45%.
Cellulose is mainly used to produce paperboard and paper.
Smaller quantities are converted into a wide variety of derivative products such as cellophane and rayon.
Conversion of cellulose from energy crops into biofuels such as cellulosic ethanol is under investigation as an alternative fuel source.
Cellulose for industrial use is mainly obtained from wood pulp and cotton.
Some animals, particularly ruminants and termites, can digest cellulose with the help of symbiotic micro-organisms that live in their guts, such as Trichonympha.
In humans, cellulose acts as a hydrophilic bulking agent for feces and is often referred to as a "dietary fiber".
Örtüaltında Organik Tarım ve İyi Tarım UygulamalarıMeltem Şahin
Örtüaltında sürdürülebilir tarım teknikleri, Organik Tarım ve İyi Tarım Uygulamalarının Karşılaştırılması yapılmıştır. Tüketici farkındalığı da küçük bir anket çalışmasıyla gösterilmiştir.
Explanation on the industrial production of penicillin covering the history, fermentors, specific conditions required for penicillin production, how to increase yield amongst others.
Green nanotechnology & its application in biomedical researchRunjhunDutta
This presentation gives detailed description of Green Nanotechnology including its principles & significance. Illustrated with examples for its application in various biomedical research fields.
A recent publication by Dr. Sachin Kadam, CTO of Advancells, sheds light on natural osteoinductive bio-compatible scaffolds for effective clinical applications on musculoskeletal disorders. These scaffolds can enhance the efficiency of stem cell transplantation, and thus improve healthcare manifold.
Microbial Processing of Agricultural Wastes to produce Pectinase Enzyme(s) an...Meesha Singh
Production of pectinase from suitable bacterial sources using agricultural wastes and market effluents as precursors.
Optimization of the physico-chemical parameters for maximum enzyme production.
The objectives of this topic are to understand, acquire, and demonstrate the concept of nanomineral synthesis, their absorption in the body, and effect on livestock productivity.
Bone defects and repair are the most common problems encountered worldwide. Bone is the second most transplanted tissue after blood. As a matter of fact, the development and progress of bone tissue engineering have focused on using artificial materials for the regeneration, repair, or restructuring of bone tissues.
3. GOAL
K.I. Uhlin, R.H. Atalla, N.S.
Thompson, Influence of
hemicelluloses on the aggregation
patterns of bacterial cellulose,
Cellulose. 2 (1995) 129–144.
doi:10.1007/bf00816385.
3
P. Wambua, J. Ivens, I. Verpoest, Natural fibres: can they replace glass in fibre reinforced plastics?, Composites Science and Technology. 63
(2003) 1259–1264. doi:10.1016/s0266-3538(03)00096-4.
4. - 1,4 β glycosidic bonds
- Most abundant natural polymer (115.55 m redwood)
(chart from assignment 2)
- Biodegradable
- Sustainable*
- http:// pubs.r s c .or g/ s er vi c e s/ i ma ge s/ RS Cp ub s. eP l a t fo r m. S er vi c e. Fr e e Co nt e nt .I ma g eS er vi c e. s vc /I ma g eS e r vi c e / Ar ti
cleima ge/ 2006 /C S/b6 01872 f/b 601872 f - f2 . gi f
- http:// ww w. r is h. kyo to - u. a c.j p/ W / LB M I/ r e s e ar c h/I a ib_ S.j p g
CELLULOSE
4
5. -Intracellular polymerization, extracellular crystallization
- Gluconacetobacter xylinus
- Bacterial Cellulose Synthase5
- activated by ci-di-GMP
- Substrate UDP-Glucose
- BcsA/B/C/D subunits from BcsI and BcsII opersons
- Mutations6
- G. xylinus NBRC 3288,(frameshift bcsBI and transposon insertion bcsCII)
- K. hansenii ATCC 23769 (IS cellulose synthase)
- Acetobacter tropicalis SKU1100 (R to S via single C deletion or insertion
polE)
- complete genome sequencing
- Basics of protein function and mechanisms
5. J .L.W. Morgan, J .T. Mcnamara, J . Zimmer, Mechanism of activation of bacterial cellulose synthase by cyclic di -
GMP, Nat Struct Mol Biol Nature Structural &Amp; Molecular Biology. 21 (2014) 489 –496.
doi:10.1038/nsmb.2803 .
6. M. Matsutani, K. Ito, Y. Azuma, H. Ogino, M. Shirai, T. Yakushi, et al., Adaptive mutation related to cellulose
producibility in Komagataeibacter medellinensis (Gluconacetobacter xylinus) NBRC 3288, Appl Microbiol
Biotechnol Applied Microbiology and Biotechnology. 99 (2015) 7229 –7240. doi:10.1007/s00253 -015-6598-x.
HISTORY: SUBCELLULAR
ci-di-GMP UDP-Glucose
5
6. 7. K. J i, W . W ang, B .
Zeng, S. Chen, Q. Zhao,
Y. Chen, et al.,
Bacteria l cellulos e
synt hes i s mecha ni s m of
facul t ati v e anaerob e
Enteroba ct e r sp. FY -07,
Sci. Rep. Scienti fi c
Reports. 6 (2016)
21863.
doi:10.1038/srep 21863 .
HISTORY: SUBCELLULAR
6
7. 5. J.L.W. Morgan, J.T. Mcnamara, J. Zimmer, Mechanism of activation of bacterial cellulose
synthase by cyclic di-GMP, Nat Struct Mol Biol Nature Structural &Amp; Molecular Biology.
21 (2014) 489–496. doi:10.1038/nsmb.2803
HISTORY: SUBCELLULAR
7
8. - Hestrin Schramm Medium8 (1954) (% w/v)
- glucose 2.0
- bactopeptone 0.5
- yeast extract 0.5
- di-sodium hydrogen phosphate* Na2HPO4 0.27
- citric acid* 0.12
- starting pH 6.0
- Modifications
-sugars (sucrose, fructose)
- Alcohols
-micronutrients (P, S, K, Mg, Ca , Fe)
- antioxidants (lignosulfonate)
- pH
8 . M. Schramm, S. Hestrin , Factors affecting Production of Cellulose at the Air/ Liquid Interface of a Culture of
Acetobacter xylinum, Journal of General Microbio lo gy. 11 (1954) 123 –129. doi:10.109 9/0 02 212 87 -1 1-1 -1 23.
https://ndb.nal .usda .go v/ ndb/ foo ds/sho w/ 87 34? fo r ma t= F u l l& re por t f m t=p df&p df Qvs= % 7 B % 7D
HISTORY: GROWTH MEDIUM
8
9. A. Kurosumi, C. Sasaki, Y. Yamashita, Y. Nakamura, Utilization of various fruit juices as carbon source for production of bacterial cellulose by Acetobacter
xylinum NBRC 13693, Carbohydrate Polymers. 76 (2009) 333–335. doi:10.1016/j.carbpol.2008.11.009.
9
Micronutrient Orange Juice Pineapple Juice
Calcium 10.89 mg 13 mg
Iron 0.20 mg 0.29 mg
Magnesium 10.89 mg 12 mg
Potassium 200 mg 109 mg
10. 10
Cellulose Specialty Uses
https://phy214uhart.wikis
paces.com/Capacitors
1. K.-Y. Lee, J.J. Blaker, R. Murakami, J.Y.Y. Heng, A. Bismarck, Phase Behavior of Medium and High Internal Phase Water-in-Oil Emulsions Stabilized Solely by
Hydrophobized Bacterial Cellulose Nanofibrils, Langmuir. 30 (2014) 452–460. doi:10.1021/la4032514.
2. M.Ö. Seydibeyoğlu, M. Misra, A. Mohanty, J.J. Blaker, K.-Y. Lee, A. Bismarck, et al., Green polyurethane nanocomposites from soy polyol and bacterial cellulose, J Mater
Sci Journal of Materials Science. 48 (2012) 2167–2175. doi:10.1007/s10853-012-6992-z.
3. K.-Y. Lee, H. Qian, F.H. Tay, J.J. Blaker, S.G. Kazarian, A. Bismarck, Bacterial cellulose as source for activated nanosized carbon for electric double layer capacitors, J
Mater Sci Journal of Materials Science. 48 (2012) 367–376. doi:10.1007/s10853-012-6754-y.
1
2
3
http://www.rubber-silanes.com/product/rubber-
silanes/en/effects/reinforcement/pages/default.aspx
12. TABLE 1.0 GROWTH MEDIUM SOURCE AND BACTERIAL FUNCTION
Element Medium Source Function
Carbon -Grade B Maple Syrup
-peptone, yeast extract
- Backbone of organic molecules
Oxygen -Oxygen in air
- organic compounds
- Last acceptor in ETC
- Organic molecules
Nitrogen - Peptone,
- Yeast extract
- Ammonium sulfate
- Proteins
- Nucleic acids
- Coenzymes
Hydrogen -organic compounds
- H2 in air
-Organic molecules
Phosphorous - Na2HPO4 (di-sodium hydrogen
phosphate)
-Nucleic acids
-Phospholipids
- Lipopolysaccharides (LPS)
Sulfur NH4SO4 - Amino Acids with Sulfur
- Coenzyme
Potassium -Grade B Maple Syrup -intracellular cation
-cytoplasmic signaling
-incorporation of sulfur into protein
- Cofactor (pyruvate kinase)
Magnesium -Grade B Maple Syrup - Cofactor (pyruvate kinase)
- Stabilizes (ATP, nucleic acids, RNA, membrane)
Calcium -Grade B Maple Syrup - Intracellular signaling hypothesized
Iron -Grade B Maple Syrup - Cytochrome ETC
12
14. - Aseptic procedure - clean bench
-Autoclave (121-140oC 2-30 minutes 15-30psi)
-70% ethanol
- Bunsen burner
-convection current
-heating inoculation loop, necks of bottles
-Monitoring
- cell number
- pH
- glucose, gluconic acid, byproduct concentration, cellulose production
-Pinpointing medium composition/concentration
http:// ww w. e xpl a i nt ha t st uf f. co m/ a uto c l a ve s. ht ml
http:// ww w. s t er il i z er s. c o m/ a u to cl a v e-t i me -t e mp e r at ur e -pr e s s ur e -c h ar t. h t ml
Table 1.0 X. Zeng, D.P. Smal l, W. Wan, Statist ica l optimiza t i on of culture conditions for bacterial cellulose
production by Acetobacter xylinum BPR 2001 from maple syrup, Carbohydrate Polymers. 85 (2011) 506 –513.
doi:10.1016/ j .ca rbp o l. 20 11. 02 .0 34.
HURDLES
14
Editor's Notes
Based on the 10 year review of BNC cellulose 10 g/L seems like a reasonable initial goal (the highest being 16.32 g/L) (mean was 7.35125 g/L)
Well defined peaks at 22.5o (002), 15o (101) and 16.8o (1,0,-1) that were also found by other papers with different carbon sources, temperatures, and bacterial strains
Crystallinity is measured using the crystallinity index which treats the peaks as diffraction from crystal lattice and minimums as scattering by amorphous structures. Generates a ratio which corresponds to the degree of crystallinity
Generally want
. high crystallinity (papers get 80-95% crystallinity)
. Ib dominant for higher order plants, Ia more common for bacteria and fungi (S. Keshk found Ia content of 0.43 and 0.44 based on FTIR Ia 750cm-1 and Ib 710 cm-1)
. S. Keshk also used X-ray diffraction (λ = 1.54 Å) with a max at I020 and a min at 2θ = 18° similar to that of the first paper
. The three numbers after the I refer to the millers index, essentially the view/slice of the lattice that the electromagnetic rays are interacting with
. d- spacing is proportional to reflective angle at a fixed wavelength
What I want is a medium that yield a large amount of cellulose that is comparable in these quantitative markers as previous papers
Economical meaning using a waste medium the as little additives as possible
Productivity
. Sucrose consumed 80% and consumption of glucose 93.5%
. Efficiency of sucrose 70% (11.98 g/L) and glucose 34%
G. Pourrameza, A. Roayaei, Q. Qezelbash, Optimization of Culture Conditions for Bacterial Cellulose Production by Acetobacter sp. 4B-2, Biotechnology(Faisalabad) Biotechnology. 8 (2009) 150–154. doi:10.3923/biotech.2009.150.154.
20 grams of carbon source per litre. Numbers in g/L4
Glucose 3.10
Mannitol 3.37
Glycerol 3.75
Fructose 2.81
Sucrose 3.83
Galactose 0.09
51.5% glucose consumption at 96h with 0.30g cellulose produced per gram consumed (down from 13.5% and 0.66%)
-Cellulose is a polymer of glucose
BNC fibril width reported to be 5.3-5.5 nm in literature 4 (Boylston and Hebert, 1980)
A bit of confusion about what product I am actually getting
.Cellulose nanofibers (CNF) width 3-50 nm, length 0.5-5.0 µm
. Bacterial nanocellulose (BNC) width 20-100 nm length 1.0-5.0 µm (width does not match up with literature)
.Cellulose nanocrystals (CNC) width 3-20 nm, length 50-1000 nm
The length of 1-5 nm is a consensus
Cellulose (35-50% wood), hemicellulose, lignin is what allow trees to stand tall same strength can be used for our purposes
Cellulose natively made by biomass and degraded by that same biomass unlike bio-based materials like PE which can not be degraded despite their source
Sustainability hinges on the effectiveness of the synthesis and processing of this cellulose
http://polysac3db.cermav.cnrs.fr/discover_cellulose.html
Cotton fibre 95% cellulose
http://puu.aalto.fi/en/midcom-serveattachmentguid-1e44f95a778f77c4f9511e4a12b01d1437b57775777/nanocelluloses.pdf
- Several genera that an synthesize cellulose, one genera goes by several names Gluconacetobacter xylinus
And Acetobacter and Komagataeibacter
BcsA polymerizes cellulose from UDP-Glucose. 8-TM helices, part of GT-2 glycosyltransferase family. C terminus has the salt bridge gating loop which covers the active site and undergoes a conformational change when bonded to (ci-di-GMP)
A and B are anchored in the periderm and synthesize the cellulose C and D and thought to transport the cellulose out of the cell in pores
A.J Brown 1886 identified ability to product cellulose
3136kb circular chromosome 3265 genes
https://microbewiki.kenyon.edu/index.php/Acetobacter_xylinum
-There are several pathways glucose can go down
. Can make other organic compounds such as amino acids, gluconic acid and pentose sugars,
. Can make energy
. Or can make cellulose
- What to get away from this is; pathways I want are energy (ETC) and cellulose
Take home message is that basics of mechanism known but not specifics
Activator to work but exactly how and where not known. But active site, salt bridge known (enough to apply)
-its all there alerady
1. K.-Y. Lee, J.J. Blaker, R. Murakami, J.Y.Y. Heng, A. Bismarck, Phase Behavior of Medium and High Internal Phase Water-in-Oil Emulsions Stabilized Solely by Hydrophobized Bacterial Cellulose Nanofibrils, Langmuir. 30 (2014) 452–460. doi:10.1021/la4032514.
2. M.Ö. Seydibeyoğlu, M. Misra, A. Mohanty, J.J. Blaker, K.-Y. Lee, A. Bismarck, et al., Green polyurethane nanocomposites from soy polyol and bacterial cellulose, J Mater Sci Journal of Materials Science. 48 (2012) 2167–2175. doi:10.1007/s10853-012-6992-z.
3. K.-Y. Lee, H. Qian, F.H. Tay, J.J. Blaker, S.G. Kazarian, A. Bismarck, Bacterial cellulose as source for activated nanosized carbon for electric double layer capacitors, J Mater Sci Journal of Materials Science. 48 (2012) 367–376. doi:10.1007/s10853-012-6754-y.
- Prices based on sigma Aldridge as well as amazon and Fischer
Seems to be standard of most papers depending on the goal
Papers used different carbon sources and addative and were trying to pinpoint the affect
Bacteria die at 145oF or 62C
-autoclave