The document discusses the history and increasing importance of automation in microbiology laboratories, particularly for blood cultures, noting that traditional culture methods can take 72 hours for results while rapid automated methods are needed to identify pathogens faster to guide antibiotic treatment for infections like sepsis. It also outlines the steps involved in optimal blood collection for cultures, including patient preparation, materials, and techniques to maximize success in collecting pediatric samples.
ELISA, Principles of ELISA, Types of ELISA- Direct ELISA
Indirect ELISA, Sandwich ELISA, Competitive ELISA, and other Types i.e. ELISPOT (enzyme-linked immunospot assay) and In-cell ELISA, Advantages and disadvantages of ELISA detection methods, Different types of microplates for ELISA, Detection strategies for ELISA
Immunological diagnosis of parasitic infectionRaghwendra sah
This slide is just made for the students who want note and don't get access to the book which cost more. So, hope you all will get information about the immunological diagnosis of parasitic infection.
Epidemiological research on brucellosis in India: Knowledge generated and gapsILRI
Presentation by Ram Pratim Deka, Johanna Lindahl and Delia Grace at a workshop on One Health approach for brucellosis control in India, New Delhi, India, 26 October 2017.
serology presentation
Serology is the scientific study of blood serum and other bodily fluids such as semen and saliva.
In practical immunological terms, serology is the diagnostic identification of antibodies in the serum.
Antibodies are typically formed in response to;
An infection, (against a given microorganism),
Other foreign proteins (blood transfusion)
Or to one’s own proteins (autoimmune disease).
The program file has been made with the vision for basic responsibilities of the Medical Microbiologists for optimal decisions in Diagnostic Microbiology, Every specimen reflects the scenario in the ongoing process of infection in the human body ( from vivo to vitro) , However it is important to know the predictive value of the tests we do in the laboratory or else the blind processing will certainly harmful if not useful Dr.T.V.Rao MD
doctortvrao@gmail.com
2021 laboratory diagnosis of infectious diseases dr.ihsan alsaimarydr.Ihsan alsaimary
2021 laboratory diagnosis of infectious diseases
dr. ihsan alsaimary
university of basrah - college of medicine- DEPARTMENT OF MICROBIOLOGY
POBOX 696 ASHAR
BASRAH 42001
IRAQ
ELISA, Principles of ELISA, Types of ELISA- Direct ELISA
Indirect ELISA, Sandwich ELISA, Competitive ELISA, and other Types i.e. ELISPOT (enzyme-linked immunospot assay) and In-cell ELISA, Advantages and disadvantages of ELISA detection methods, Different types of microplates for ELISA, Detection strategies for ELISA
Immunological diagnosis of parasitic infectionRaghwendra sah
This slide is just made for the students who want note and don't get access to the book which cost more. So, hope you all will get information about the immunological diagnosis of parasitic infection.
Epidemiological research on brucellosis in India: Knowledge generated and gapsILRI
Presentation by Ram Pratim Deka, Johanna Lindahl and Delia Grace at a workshop on One Health approach for brucellosis control in India, New Delhi, India, 26 October 2017.
serology presentation
Serology is the scientific study of blood serum and other bodily fluids such as semen and saliva.
In practical immunological terms, serology is the diagnostic identification of antibodies in the serum.
Antibodies are typically formed in response to;
An infection, (against a given microorganism),
Other foreign proteins (blood transfusion)
Or to one’s own proteins (autoimmune disease).
The program file has been made with the vision for basic responsibilities of the Medical Microbiologists for optimal decisions in Diagnostic Microbiology, Every specimen reflects the scenario in the ongoing process of infection in the human body ( from vivo to vitro) , However it is important to know the predictive value of the tests we do in the laboratory or else the blind processing will certainly harmful if not useful Dr.T.V.Rao MD
doctortvrao@gmail.com
2021 laboratory diagnosis of infectious diseases dr.ihsan alsaimarydr.Ihsan alsaimary
2021 laboratory diagnosis of infectious diseases
dr. ihsan alsaimary
university of basrah - college of medicine- DEPARTMENT OF MICROBIOLOGY
POBOX 696 ASHAR
BASRAH 42001
IRAQ
Basic laboratory procedures in clinical bacteriologyamin beni
book of Basic laboratory procedures in clinical bacteriology Basic laboratory procedures in clinical bacteriology Basic laboratory procedures in clinical bacteriology Basic laboratory procedures in clinical bacteriology
Biochemistry is a basic science which deals with chemical nature and chemical behaviour of living matter and with the reactions and processes they undergo.
Biochemistry involves the study of:
Chemical constituents of living matter.
Chemical changes which occur in the organism during digestion, absorption and excretion.
Chemical changes which occur during growth and multiplication of the organism.
Transformation of one form of chemical constituent to the other.
Energy changes involved in such transformation.
Note:- The term “Biochemistry” was first introduced by German chemist Carl Neuberg in 1903 from Greek word “bios” means “life”.
It is mainly deals with the biochemical aspects that are involved in several conditions.
The results of qualitative and quantitative analysis of body fluids assist the clinicians in the diagnosis, treatment and prevention of the disease and drug monitoring, tissue and organ transplantation, forensic investigations and so on.
Various biological fluids subjected to chemical tests and assays include blood, plasma, serum, urine, cerebrospinal fluid (CSF), ascetic fluid, pleural fluid, faeces, calculi and tissues.
Note:- Modern day medical practice is highly dependent on the laboratory analysis of body fluids, especially the blood. The disease manifestations are reflected in the composition of blood and other tissues.
Hence, the demarcation of abnormal from normal constituents of the body is another aim of the study of clinical biochemistry.
ANY WASTE GENERATED DURING THE DIAGNOSIS, TREATMENT OR IMMUNIZATION OF HUMA...ssuser3155141
BIOMEDICAL WASTE
IS DEFINED AS
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THE DIAGNOSIS, TREATMENT
OR IMMUNIZATION OF HUMANS
OR ANIMALS OR IN RESEARCH
ACTIVITIES PERTAINING THERTO
OR IN THE
PRODUCTION OR
TESTING OF BIOLOGI
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Tata Group Dials Taiwan for Its Chipmaking Ambition in Gujarat’s DholeraAvirahi City Dholera
The Tata Group, a titan of Indian industry, is making waves with its advanced talks with Taiwanese chipmakers Powerchip Semiconductor Manufacturing Corporation (PSMC) and UMC Group. The goal? Establishing a cutting-edge semiconductor fabrication unit (fab) in Dholera, Gujarat. This isn’t just any project; it’s a potential game changer for India’s chipmaking aspirations and a boon for investors seeking promising residential projects in dholera sir.
Visit : https://www.avirahi.com/blog/tata-group-dials-taiwan-for-its-chipmaking-ambition-in-gujarats-dholera/
Memorandum Of Association Constitution of Company.pptseri bangash
www.seribangash.com
A Memorandum of Association (MOA) is a legal document that outlines the fundamental principles and objectives upon which a company operates. It serves as the company's charter or constitution and defines the scope of its activities. Here's a detailed note on the MOA:
Contents of Memorandum of Association:
Name Clause: This clause states the name of the company, which should end with words like "Limited" or "Ltd." for a public limited company and "Private Limited" or "Pvt. Ltd." for a private limited company.
https://seribangash.com/article-of-association-is-legal-doc-of-company/
Registered Office Clause: It specifies the location where the company's registered office is situated. This office is where all official communications and notices are sent.
Objective Clause: This clause delineates the main objectives for which the company is formed. It's important to define these objectives clearly, as the company cannot undertake activities beyond those mentioned in this clause.
www.seribangash.com
Liability Clause: It outlines the extent of liability of the company's members. In the case of companies limited by shares, the liability of members is limited to the amount unpaid on their shares. For companies limited by guarantee, members' liability is limited to the amount they undertake to contribute if the company is wound up.
https://seribangash.com/promotors-is-person-conceived-formation-company/
Capital Clause: This clause specifies the authorized capital of the company, i.e., the maximum amount of share capital the company is authorized to issue. It also mentions the division of this capital into shares and their respective nominal value.
Association Clause: It simply states that the subscribers wish to form a company and agree to become members of it, in accordance with the terms of the MOA.
Importance of Memorandum of Association:
Legal Requirement: The MOA is a legal requirement for the formation of a company. It must be filed with the Registrar of Companies during the incorporation process.
Constitutional Document: It serves as the company's constitutional document, defining its scope, powers, and limitations.
Protection of Members: It protects the interests of the company's members by clearly defining the objectives and limiting their liability.
External Communication: It provides clarity to external parties, such as investors, creditors, and regulatory authorities, regarding the company's objectives and powers.
https://seribangash.com/difference-public-and-private-company-law/
Binding Authority: The company and its members are bound by the provisions of the MOA. Any action taken beyond its scope may be considered ultra vires (beyond the powers) of the company and therefore void.
Amendment of MOA:
While the MOA lays down the company's fundamental principles, it is not entirely immutable. It can be amended, but only under specific circumstances and in compliance with legal procedures. Amendments typically require shareholder
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It is a sample of an interview for a business english class for pre-intermediate and intermediate english students with emphasis on the speking ability.
[Note: This is a partial preview. To download this presentation, visit:
https://www.oeconsulting.com.sg/training-presentations]
Sustainability has become an increasingly critical topic as the world recognizes the need to protect our planet and its resources for future generations. Sustainability means meeting our current needs without compromising the ability of future generations to meet theirs. It involves long-term planning and consideration of the consequences of our actions. The goal is to create strategies that ensure the long-term viability of People, Planet, and Profit.
Leading companies such as Nike, Toyota, and Siemens are prioritizing sustainable innovation in their business models, setting an example for others to follow. In this Sustainability training presentation, you will learn key concepts, principles, and practices of sustainability applicable across industries. This training aims to create awareness and educate employees, senior executives, consultants, and other key stakeholders, including investors, policymakers, and supply chain partners, on the importance and implementation of sustainability.
LEARNING OBJECTIVES
1. Develop a comprehensive understanding of the fundamental principles and concepts that form the foundation of sustainability within corporate environments.
2. Explore the sustainability implementation model, focusing on effective measures and reporting strategies to track and communicate sustainability efforts.
3. Identify and define best practices and critical success factors essential for achieving sustainability goals within organizations.
CONTENTS
1. Introduction and Key Concepts of Sustainability
2. Principles and Practices of Sustainability
3. Measures and Reporting in Sustainability
4. Sustainability Implementation & Best Practices
To download the complete presentation, visit: https://www.oeconsulting.com.sg/training-presentations
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https://viralsocialtrends.com/vat-registration-outlined-in-uae/
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Implicitly or explicitly all competing businesses employ a strategy to select a mix
of marketing resources. Formulating such competitive strategies fundamentally
involves recognizing relationships between elements of the marketing mix (e.g.,
price and product quality), as well as assessing competitive and market conditions
(i.e., industry structure in the language of economics).
Falcon stands out as a top-tier P2P Invoice Discounting platform in India, bridging esteemed blue-chip companies and eager investors. Our goal is to transform the investment landscape in India by establishing a comprehensive destination for borrowers and investors with diverse profiles and needs, all while minimizing risk. What sets Falcon apart is the elimination of intermediaries such as commercial banks and depository institutions, allowing investors to enjoy higher yields.
2. Beginning of Microbiology
Almost exactly 300
years ago Anton van
Leeuwenhoek
described the first
bacteria seen through
the microscope, thus
providing the technical
basis for studying the
morphology of microorganisms
3. Knowledge explosion in
Microbiology
The explosion of knowledge in the last
century, pioneered by Pasteur, Koch and
many others immortalised in modern
generic and specific names was
dependent on improvements in
procedures for isolating and identifying
organisms of importance in industry and
medicine.
4. Rapid Methods are
Emerging
Rapid methods and automation is a
dynamic area in applied microbiology
dealing with the study of improved
methods in the isolation, early detection,
characterization, and enumeration of
microorganisms and their products in
clinical, food, industrial, and
environmental samples.
5. Changing perceptions ,
Microbiology labs are beginning to accept
the shift toward automation for reasons as
numerous and varied as microbiology
itself. Microbiologists and lab technicians
recognize that automated solutions are
not intended to replace cognitive decisionmaking but rather, simply replace tedious,
repetitive steps.
6. Man has Evolved So also the
Microbes, so the need for
Automation
7. Beginning of Automation
The field started around mid-1960s and
with the development of a variety of
miniaturized microbiological techniques
developed
Streamlining workflow maintains
consistency but allows microbiologists to
devote more time to operations that require
their unique skills and experience
8. Hospital Acquired Infection a threat to
Medical Profession- needs faster
methods to Identify.
Microbiology labs play a crucial role by
establishing a front line of defence against
the threat of Nosocomial infections.
According to the Centres for Disease
Control and Prevention, in American
hospitals alone, HAIs account for an
estimated 1.7 million infections and
99,000 associated deaths annually. Of
these infections, 32% are urinary-tract,
22% are surgical-site, 15% are
pneumoniae, and 14% are bloodstream.
9. The Clinicians need Quicker
Results
The shift from
manual test
processing to
automated solutions
can aid in reducing
HAIs by providing
health systems with
standard, consistent
lab processing that
yields quicker, more
accurate test results.
10. Automation enters into several
areas in Microbiology
Rapid Methods and Automation in
Microbiology has developed into an
important sub-discipline of applied
microbiology in the past 15 years. The
field deals with improved methods in the
isolation, early detection, characterization,
and enumeration of microorganisms and
their products in clinical, food, industrial,
and environmental samples.
11. Automation reduces errors and
innovative
Automated processes have gone well
beyond changing outdated procedures.
They minimise potentially dangerous
practices, lower turnaround time, reduce
errors, enhance quality control, improve
specimen handling and boost accuracy.
Moreover, technologists in automated
labs tend to embrace innovation more
readily.
12. Lesser Manpower More volume
of work
Because automation largely circumvents
repetitive manual processing, lab
technicians find it easier to focus on
complex tasks that require their specific
skills.
It is therefore remarkable that, despite
shortages of skilled personnel and
increases in the volume of work, it has
taken years for automation to become
acceptable to microbiologists.
13. Desired Objectives in Automation
Reexamination of laboratory functional steps
–
–
–
–
Phlebotomy
Sample labeling
Transportation
Pre-, peri-, and post-analytical processing
Laboratory automation for improved efficiency and
error reduction
Create an informatics continuum
– Process Control vs. LIS function
– Auto-verified and auto-interpreted data
Predictive genomics and the passive home
monitoring paradigm
14. Adding Value to Lab Tests Through Automation
Lab Test
– Faster TOT
– Accuracy,
Precision, Safety
Add information
value
– Auto validation
– Trending
Effecting
change using
lab results
– Lifestyle
changes
– Selection of
therapeutics
Lab Test
Auto validation
Trending
Life Style Adjustments
Appropriate Therapeutics
15. Every Body is a Learner to New
Technologies, Ent hus i as m
Makes Difference
16. Blood culturing most
important and live saving
Investigation
Needs optimal Methods for
Diagnosis of Blood Borne
Pathogens
17. Why Blood Culture
Physician
infection
can find source of
Physician
can initiate lifesaving support measures
Physician
can start targeted
antibiotic therapy
18. What is a Blood Culture?
A blood culture is a
laboratory test in
which blood is
injected into bottles
with culture media to
determine whether
microorganisms have
invaded the patient’s
bloodstream.
19. Need for Blood Culture?
No microbiological test is more essential to the
clinician than the blood culture. The finding of
pathogenic microorganisms in a patient’s
bloodstream is of great importance in terms of
diagnosis, prognosis, and therapy.”
- L. Barth Reller, Clin. Infect. Diseases, 1996
20. A
Proof in Blood borne
Infection
clinically suspected infection is
ultimately confirmed by isolation or
detection of the infectious agent.
Subsequent identification of the
microorganism and antibiotic susceptibility
tests further guide effective antimicrobial
therapy. Bloodstream infection is the most
severe form of infection and is frequently
life-threatening, and blood culture to
detect circulating microorganisms has
been the diagnostic standard.
21. Blood culture is a Important a
Diagnostic tool in Infectious
diseases
Blood
culture is a microbiological
culture of blood. It is employed to
detect infections that are spreading
through the bloodstream
(such as bacteraemia, septicaemia
This is possible
because the bloodstream is usually
a sterile environment.
amongst others).
22. What are We doing Now is not
optimal – Needs Rapid Methods
Most microbiological culture procedures
require the use of solid media, like blood
agar and MacConkey agar plates that
need to be visually monitored by trained
personnel at intervals of 24 hours. These
conventional cultures using normal media
take at least a minimum of 72 hours to
isolate the pathogen and carry out
susceptibility test to know the efficacy of
antibiotics on simple aerobic bacteria
23. Optimal Methods of Blood
Collection makes difference
The physicians consent with filled in request with details
for culturing the Blood
Gloves will be worn in accordance with standard
precautions..
Appropriate verification of the patient's identity, by
means of an armband or area specific procedure, will
occur before the specimen collection.
Cultures should be drawn before administration of
antibiotics, if possible.
If at all possible, blood cultures should not be drawn from
lines, but should be drawn only via venepuncture
25. NEW CATEGORY OF
LAB PERSONNEL
Phlebotomist
Defined as a person who collects blood for
clinical laboratory test or examination
purposes
26. Principles for Collection
Gloves will be worn in accordance with standard
precautions.
•A physician’s order must be obtained for specimen
collection.
•Appropriate verification of the patient's identity, by
means of an armband or area specific procedure, will
occur before the specimen collection.
•Cultures should be drawn before administration of
antibiotics, if possible.
•If at all possible, blood cultures should note drawn from
lines, but should be drawn viavenipuncture.
27. Materials
Chlorhexidine swabs (1-2 packages)
Alcohol swabs
Blood culture bottles (2 bottles per set)
2 syringes (adult: 20 cc, paediatric: 5 cc)
2 needles (adult: 22 gauge or preferably larger butterfly
or standard needle; pediatric: 25 or 23 gauge butterfly or
standard needle)
Gloves (sterile &nonsterile)
Tourniquet
Sterile gauze pad
Adhesive strip or tape
Self-sticking patient labels
Plastic zip lock specimen bags
28. Steps 1 – 3, Check, Explain,
Wash
1.Identify the patient by
checking the arm band or
area-specific procedure.
2.Explain the procedure
to the patient.
3.Wash hands with soap
and water with friction for
15 seconds or use
alcohol based hand rub
29. Step 4 –Prep Cap
Prep the rubber cap
of the blood culture
bottles with an alcohol
pad in a circular
motion. Allow the
alcohol to dry.
30. Step 5 -Prep the Puncture Site
Prep the puncture site with Chlorhexidine:
– •Using aseptic technique, remove the applicator from
its package.
– •Holding the applicator downward, gently squeeze the
wings to release the solution.
– •Scrub with a back & forth motion using friction for 30
seconds on dry skin or 2 minutes on wet skin.
•Do not wipe the site after cleansing the skin
with Chlorhexidine.
31. Step 6 -Gloves
Apply gloves:
If palpation of site prior
to puncture is
anticipated, wear sterile
gloves.
If palpation of site prior to
puncture is not
anticipated, wear
nonsterile gloves.
32. Step 8 -Mix
Gently rotate the
bottles to mix the
blood & the broth (do
not shake vigorously).
33. Step 9 and 10 (Label)
Place the patient label on each bottle & label
each culture bottle with the site of specimen
collection. When applying patient identification
labels, do not cover the bar code label on the
blood culture bottles. Attach the laboratory
requisition.
Send the blood cultures to the Clinical
Microbiology receiving area as soon as possible.
34. Step 11
11.Document
the following in the medical
record
Date & time specimen obtained
–Site of specimen collection
If 2 sets of blood cultures have been
ordered, obtain the second set in the
same manner as the first, making a new
venepuncture at a different site
35. Techniques of Paediatric Collections
Tourniquet Application
Need to maximize chances of successful
collection
Remember that the vein is still developing and
might need to rely on firm tightness
Ideally, tourniquet should not be kept on for
more than one minute
If possible, apply heat
If using a hand, consider a bucket of warm
water
36. Techniques of Paediatric Collections
Insertion Principles
Cantilevering of elbow
The option factor:
-Choose your options of direction before insertion
-Minimize the odds of unnecessary “digging”
Avoid plunging the needle right up to the hilt
Often, a drawback does the trick
If vacutainer is slowing down, replace with a
syringe
37. Self Protection
A few ways to make sure your
role in the collection process is
carried out with efficiency,
orderliness and safety
38. The Contaminated Blood
Culture
If the skin is not adequately cleansed before
drawing blood for culture, bacteria on the skin will
be injected into the bottle, producing a false
positive blood culture.
It is sometimes difficult for the physician to
determine whether the bacteria growing in the
blood culture is a real pathogen causing
bloodstream infection or whether bacteria on the
skin have contaminated the culture. This can lead
to excess use of antibiotics and prolongation of
hospital stay.
39. Sample Labeling
Efficiencies
Bar coding at the point-
of-phlebotomy
2D vs. 1D bar codes
– Reduce the number
of computer
interfaces
– Self directing
specimens
B-D id
40. Technological Improvements for All
Steps in the Diagnostic Process
Just in Time Supplies
Automated
Phlebotomy
Trays
Process Control
EMR
Reporting
Instruments
Designed for
Automation
Storage
RFID
2D-Codes
Recording
Biorepository
Transportation
Analysis
Pre-analytical
Analytical and
Automation
Mobile Robot
Accessioning
41. What is a Blood Culture?
A blood culture is a
laboratory test in
which blood is
injected into bottles
with culture media to
determine whether
microorganisms have
invaded the patient’s
bloodstream.
42. Blood & Body Fluid Cultures
Blood cultured by the BacT/Alert 3D leads to early
detection of pathogens (>89 per cent within 24 hours
and 97 per cent within 48 hours) especially in cases of
septicaemia, enteric fevers, bacterial endocarditis and
other pyrexias of bacterial origin.
Activated charcoal neutralises antimicrobials and toxins
enhancing early recovery of pathogens. Positives are
detected faster than Bactec even at low concentrations
in blood and body fluids like CSF, CT guided aspirates
etc.
Delayed transport does not compromise results.
The instrument is capable of recovering significantly
more organisms that resin.
43. BacT/AlerT 3D culture
system
BacT/AlerT 3D culture system. This is the
first automated non-radiometric and noninvasive culture system that continuously
monitors system for culture of bacteria
(both aerobic and anaerobic), fungi and
mycobacteria. All these bacteria can be
cultured using different media as
prescribed..
44. Principles in BacT/AlerT 3D
culture system
This is a closed system and works on the
colorimetric principle of detection of CO2
produced by the organisms. The CO2
causes a lowering of the pH of the
medium, which in turn produces a colour
change in a sensor attached to the CO2sensitive base of each bottle.
45. You are guided by
Computerized Systems
The instrument reacts before this colour
change is apparent by means of an
audible or visible alert flagged by the
computer. The bottles are constantly
agitated and are read at 10-minute
intervals. The readings are transmitted to
a computer compiler, which computes
results. This
46. bioMérieux BacT/ALERT® 3D
The bioMérieux BacT/ALERT® 3D provides
an optimal environment for the recovery of a
wide range of pathological organisms,
including bacteria, yeasts and
mycobacteria; utilizing proprietary plastic
culture bottles ensuring added safety to the
user.
47. Principles of functioning of BacT alert
Monitors
Microorganisms multiply
in the media, generating
CO2. As CO2 increases,
the sensor in the bottle
turns a lighter colour.
Measuring reflected light,
the BacT/ALERT 3D
monitors and detects
color changes in the
sensor.
Algorithms analyze the data to
determine positivity, and the
laboratory is notified
immediately with visual and
audible alarms.
49. Automation becomes need of the
Hour
Full microbiology laboratory automation
needs have never been so apparent, with
financial constraints and increasing testing
volumes at the same time that labour is
becoming both harder to find and more
expensive. Implementation of full
microbiology lab automation is one
solution, as fewer technologists are
required to process automated tests..
50. Automation improves quality
of services
Overall, laboratories
transitioning from
conventional to
automated processes
find that technologists
and microbiologists
are more open to
innovation and
improved quality.
51. Industry flourishes too..
An entire industry of
microbial diagnostic kits
flourished to the present
day. Next in the 70s the
development was in
immunological test kits
and instruments to
monitor the presence of
food borne pathogens
and biomass and to
predict microbial growth
automatically.
52. Advantages of automation
Automated solutions have recently
emerged in the marketplace that address
key areas of the microbiology lab.
Automating these processes-simple,
standard, or complex-can revolutionize
the microbiology lab with more efficient,
standardized practices that will improve
quality, safety, and cost-efficiency.
53. Automation increases efficacy and
eliminates individual variations
For example, automating small, yet vitally
important tasks, can make a huge impact
on the efficiency and accuracy of
laboratories. Lab technicians streak an
estimated ??? agar plates a day, a
process that is laborious, tedious, and
inconsistent. Each lab technician has his
own streaking technique
54. Automation is
Advantageous
Include the elimination of subjective
variability,
Savings in media and reagents, and the
earlier production of useful information in
many instances, all of winch can make a
substantial contribution to productivity and
the control of runaway cost escalation.
55. Automation combined with Laboratory
Information management
The
combined use of laboratory
automation and laboratory
information management software
(LIMS) has been shown to increase
productivity, reduce human error
and improve tracking and
traceability in a microbiology lab
57. Bar coding, robotics and computers
Bar coding, robotics and computers that replace
manual transcription significantly reduce data
loss and errors. Automation also makes it less
likely that plate information and patient
identification will be duplicated or transposed.
Three trends will drive laboratory automation’s
future: smaller, more-flexible analysers and
automation based on next-generation
technology, including micro fluidics, easy-to-use,
powerful software for centralised lab
management, and internet-based real-time
service for better up-time.
58. Bar coding replacing the
Manual reading – Reduces
errors
The key to this real-time automation was
real time barcode labelling of all sample
carriers (such as bags, tubes, dishes,
bottles) provided by Kiestra's Barcode
system. This was used in conjunction with
Auto scribe's Matrix LIMS. Real time
barcode reading is known to reduce
transcription error rates to only 1 in 36
trillion characters - compared to 1 in 300
characters with manual reading.
60. Bar-coding helps in tracing
the errors
The barcode makes
every sample carrier
unique and recorded
meaning full
traceability for the
laboratory. Samples
are also provided with
a barcode which is
generated by Matrix
LIMS.
61. Quality replacing Quantitity
Quality
issues are becomin g in creasin gly
important in diagnostic laboratories. The fact of
quality is no longer sufficient and we must now
develop mechanisms to assure consumers, the
public and, most importantly, ourselves of the
contin uin g quality of our service. Movin g
towards a quality -assured system is not easy,
requirin g a meticulous attention to detail in all
areas of a laboratory's workin g and
organization.
62. Automation gained the
Universal acceptance
A further plus for lab automation is that it
promotes consistency and quality. Without
automation, lab tasks that are necessarily
repetitive can lead to inconsistent or
inappropriate ways of work and, from
there, to improper treatment, longer
patient stays, medication errors and
unwanted drug side-effects.
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The learning objectives for this presentation include a reexamination of the functional steps that are required to provide a laboratory result to a physician. These steps include phlebotomy sample labeling, transportation, centrifugation, pre-analytical processing. We will look at laboratory automation as a means to improve efficiency and error reduction not only throughout the pre-analytical and analytical steps in the diagnostic process, but in the post analytical area as well. What I hope to convey is that there is an opportunity to create an informatics continuum by monitoring and directing all steps in the process, the need to evaluate the current capabilities of your laboratory information system (LIS) and to learn how medical decision making can be enhanced by the use of an informatics continuum.
In the future, diagnostic data will be gathered on a continuous basis:
- Ubiquitously in the home to establish wellness routines and to forecast the onset of disease.
- Following the appearance of chronic and acute conditions, then point of care systems will be used to enhance the diagnostic process. Once professional medical intervention is necessary, then esoteric testing will be performed in the Core Laboratory.
- The data continuum will be autoverified and interpreted automatically, where possible, and then only the more difficult cases will be sent to medical professionals.
The electronic medical record is the gathering point for all the medical information. Not only is the electronic medical record a storage location (much like a patient chart), but it is also a point where data interpretation can be enhanced by the juxtaposition of so many diagnostic measures.
It is essential that the laboratory embraces the notion that it can play a major role in creating and maintaining the diagnostic knowledge engine that drives much of the electronic medical record. Thus the laboratory will become an essential partner in clinical care.
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Automation can add value to what we currently think of as our end product, a lab test result. Automation can provide faster turnaround time, improved accuracy, precision and safety. Information value can be enhanced by auto-validation of the results and also by providing the physician with trending information. If properly reported in context with the patients current health condition, lab results can encourage healthy lifestyle changes and ultimately the selection of appropriate therapeutics.
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However, significant gains can be made in terms of sample processing simply by adopting new labeling methods. Bar coding at the point of phlebotomy is still only practiced by less than 50% of hospitals. Becton Dickenson has had their bar coding system on the market for many years that allows bedside bar code scanning and printing, and assures proper label placement. In the future we will use 2D bar codes which will be easier to read, and allow greater data densities than one dimensional codes. Ultimately, the radio frequency ID chip will be attached to every tube once the price drops to less than 5 cents a label.
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Of the 166 separate steps associated with getting a specimen into the laboratory, there is a technology that can improve the process. Automation can provide just in time supplies to the phlebotomy process, improved labeling of the specimen to assure rapid transportation to the appropriate analytical system using mobile robots or automated pneumatic tube systems. Pre-analytical processors can automatically complete most of the accessioning and pre-analytical processing steps. Instruments designed to operate with automation systems can rapidly sample the specimen and release it for further processing. Intelligent test reporting can be implemented at the end of an efficient workflow process using autoverification, and automated test expansion (for example, repeat, reflex, and add-on testing). Finally, process control software systems can monitor and optimize every step in the process to make sure the result is accurate, timely, and is posted securely to the electronic medical record.
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Process control allows more sophisticated management of the laboratory process.
Automation is responsible for:
Sample quality assessment at the beginning of the process
Optimized specimen routing and scheduling
Intelligent reporting
Information management includes the:
Accessioning process
Specimen tracking
Data logging and reporting
Quality control documentation