Assignment 02152011

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The document describes the structure of a complex between the Escherichia coli replication terminator protein (TUS) and 13 base pairs of double-stranded DNA. The TUS protein locks the DNA in a bent conformation with an unstretched length of 4.135 nm. The whole TUS-DNA complex is approximately 5.2 nm by 7 nm by 4.78 nm in size and shows the TUS protein (colored pink, orange, purple and white) binding along the DNA in the major and minor grooves.

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Escherichia Coli Replication Terminator Protein (TUS) Complexed With DNA - Locked form

Minor groove ,[object Object],with unstretched length of 4.135 nm and width from backbone to backbone of 1.55-2.5nm. The base pair length is .389nm. Appearance of major and minor grooves depends on the orientation of the DNA. Major groove

[object Object],Pink, orange purple and white strings are the proteins. The whole complex is 5.2 nm X 6.98 nm X 4.78 nm. The little balls are the molecules.

[object Object],Complex under Van der wall surface.

[object Object],looking at the transverse plane normal to DNA’s axis. I am not sure.

The inherited DNA of an organism contains specific traits that dictate the synthesis of proteins through the RNA molecules involved in protein synthesis. In other words, proteins are the link between genotype and phenotype. Gene expression is the process by which DNA directs the synthesis of proteins (or, in some cases, just RNAs). The expression of genes that code for proteins includes two stages: transcription and translation. In this slide, we are going to take a look into the processes of replication and transcription. DNA ارثی یک ارگانیسم حاوی صفات خاصی است که سنتز پروتئین‌ها را از طریق مولکول‌های RNA درگیر در سنتز پروتئین دیکته می‌کند. به عبارت دیگر، پروتئین‌ها رابط بین ژنوتیپ و فنوتیپ هستند. بیان ژن فرآیندی است که در آن DNA سنتز پروتئین‌ها (یا در برخی موارد فقط RNA ها( را هدایت می‌کند. بیان ژن‌هایی که برای پروتئین‌ها کد می‌کنند شامل دو مرحله است: رونویسی و ترجمه. در این اسلاید قصد داریم نگاهی به فرآیندهای همانندسازی و رونویسی بیاندازیم.

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Unlike DNA replication in the cell, PCR uses heat to separate DNA strands. (T/F) TRUE ======================================================= Restriction endonucleases are enzymes that cut DNA at specific double-stranded sequences. (T/F) TRUE ================================================ Toq is a type of DNA polymerase used in PCR because it is thermo-stable (stable at high heat). (T/F) TRUE ================================================= A bacteriophage is a kind of virus that can be used as a cloning vector. (T/F) TRUE ============================================================ An ori site a region of plasmid DNA containing antibiotic resistance False ============================================================ The GFP is an example of \"reporter protein\" naturally found in bacteria. (T/F) True =================================================================== Reverse transcriptaseis an enzyme that generates \"sticky ends.\" True =====================================================================(cid:13)(cid:10) DNA ligase is an enzyme that generates \"sticky ends.\" (T/F) true =====================================================================(cid:13)(cid:10) A polylinker is a segment of plasmid DNA that contains multiple restriction enzyme sites. (T/F) True =====================================================================(cid:13)(cid:10) Antibiotic resistance gene can be identify bacterial colonies containing recombinant DNA FALSE ===================================================================== === Eukaryotic genes can be expressed in bacteria using an appropriate promotor True ===================================================================== == The initial phase in PCR is to warm the blend to a high temperature, as a rule 94 to 95 °C, for around five minutes. The hydrogen securities that hold together the two strands of a twofold helix are broken at these temperatures, and the DNA isolates into single strands. This procedure is named denaturation. ======================================================= A confinement chemical or limitation endonuclease is a compound that cuts DNA at or close particular acknowledgment nucleotide arrangements known as limitation locales. Limitation compounds are ordinarily ordered into four sorts, which vary in their structure and whether they cut their DNA substrate at their acknowledgment site, or if the acknowledgment and cleavage locales are separate from each other. To cut DNA, all confinement catalysts make two entry points, once through every sugar-phosphate spine (i.e. every strand) of the DNA twofold helix. These chemicals are found in microorganisms and archaea and give a guard component against attacking viruses.Inside a prokaryote, the confinement proteins specifically cut up outside DNA in a procedure called limitation; then, have DNA is ensured by an alteration compound (a methyltransferase) that adjusts the prokaryotic DNA and squares cleavage. Together, these two pro.

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