Seminario BM
•Download as PPTX, PDF•
0 likes•236 views
The study aimed to determine if using caspase inhibitors in pulsed-field gel electrophoresis (PFGE) could improve the estimation of radiation-induced DNA repair and apoptosis. PFGE is a technique used to examine chromosomal DNA and separate DNA fragments of different sizes. The study exposed tumor cells to radiation and used PFGE along with caspase inhibitors to examine DNA fragmentation and apoptosis during the repair process. The results and conclusions suggested that new techniques combining caspase inhibitors and PFGE may provide better and more reliable results for understanding radiation-induced DNA repair and programmed cell death.
Report
Share
Report
Share
Recommended
pcr & dystrophin gene
The document discusses PCR and the dystrophin gene. PCR is used to amplify small sections of DNA or genes. It works by repeating cycles of denaturing DNA, annealing primers, and extending the primers to make copies of the target sequence. Mutations in the dystrophin gene, which is located on the X chromosome and provides instructions for the dystrophin protein, can cause Duchenne muscular dystrophy (DMD). Dystrophin links the inside and outside of muscle membranes and protects muscles from injury. Multiplex PCR is used to screen for exon deletions in the dystrophin gene that cause DMD.
DNA Footprinting
Hello There,
DNA Footprinting Is A Molecular Biology Technique With Wide Applications In Many Areas Of Biological Sciences And Importantly It Is Used For Crime Detection In Forensic Sciences. In This Presentation, You Will Learn What It Is, The Technology, Protocol, Pictorial Representation, Applications And References For Further Study.
Practice Candidacy Exam
This document provides an overview of shotgun DNA mapping using single molecule DNA analysis techniques. It discusses digesting genomic DNA into random fragments, constructing DNA tethers for optical tweezers unzipping, simulating unzipping profiles to create a library for comparison to experimental data, and potential applications for studying chromatin structure, transcription, and telomeres. The goal is to map DNA sequences to their genomic locations in order to better understand protein-DNA interactions and gene expression at the single molecule level.
D nase, dms, microarray
This document discusses three techniques for analyzing DNA-protein interactions: DNase footprinting, DMS footprinting, and microarrays. DNase footprinting uses DNase I enzyme to cleave DNA, which is then resolved by gel electrophoresis to identify protein-protected regions. DMS footprinting uses dimethyl sulfate to modify DNA bases, and reverse transcription to detect modifications that indicate protein binding sites. Microarrays immobilize DNA probes on chips to detect target sequences through fluorescent hybridization, allowing analysis of gene expression profiles. These techniques provide insights into protein-DNA binding and structural changes in nucleic acids.
SNP Detection Methods and applications
complete Single Nucleotide Polymorphiitsm Detection methods with Advance techniques with its applications
Single nucleotide polymorphisms are single base variations between genomes within a species.
There are at least 10 million polymorphic sites in the human genome.
SNPs can distinguish individuals from one another
Denaturing Gradient Gel Electrophoresis
Chemical Cleavage Of Mismatch
Single-stranded Conformation Polymorphism (SSCP)
MutS Protein-binding Assays
Mismatch Repair Detection (MRD)
Heteroduplex Analysis (HA)
Denaturing High Performance Liquid Chromatography (DHPLC)
UNG-Mediated T-Sequencing
RNA-Mediated Finger printing with MALDI MS Detection
Sequencing by Hybridization
Direct DNA Sequencing
Single-feature polymorphism (SFP)
Invader probe
Allele-specific oligonucleotide probes
PCR-based methods
Allele specific primers
Sequence Polymorphism-Derived (SPD) markers
Targeting induced local lesions in genomes (TILLinG)
Minisequencing primers
Allele-specific ligation probes
Molecular Markers
Dr. S. MANIKANDAN, M.Sc., Ph.D
Lecturer in Botany
Thiruvalluvar University Model Constituent College,
Tittagudi 606 106, Tamil Nadu, India.
Email id: drgsmanikandan@gmail.com
Genomica - Microarreglos de DNA
The document discusses various applications and techniques of DNA microarrays, including summarizing key points about Affymetrix GeneChips, spotted microarrays, experimental design, data analysis, and several case studies on various topics like ovarian cancer, Sjogren's syndrome, wine yeast genomics, and norovirus genotyping. Microarrays allow analysis of gene expression patterns and copy number variations across genomes through comparative hybridization experiments. The document provides an overview of microarray technology and applications in genomic and biomedical research.
Lecture 28
The document summarizes key concepts in molecular genetics including DNA repair mechanisms using nucleases and DNA polymerase, telomeres and their role, transcription which involves copying DNA into mRNA with RNA, differences between RNA and DNA like the sugar and nucleotide uracil, codons and their role in specifying amino acids, and Marshall Nirenberg's experiments concluding that DNA is a universal genetic code and mRNA acts as a template from its 5' to 3' end.
Recommended
pcr & dystrophin gene
The document discusses PCR and the dystrophin gene. PCR is used to amplify small sections of DNA or genes. It works by repeating cycles of denaturing DNA, annealing primers, and extending the primers to make copies of the target sequence. Mutations in the dystrophin gene, which is located on the X chromosome and provides instructions for the dystrophin protein, can cause Duchenne muscular dystrophy (DMD). Dystrophin links the inside and outside of muscle membranes and protects muscles from injury. Multiplex PCR is used to screen for exon deletions in the dystrophin gene that cause DMD.
DNA Footprinting
Hello There,
DNA Footprinting Is A Molecular Biology Technique With Wide Applications In Many Areas Of Biological Sciences And Importantly It Is Used For Crime Detection In Forensic Sciences. In This Presentation, You Will Learn What It Is, The Technology, Protocol, Pictorial Representation, Applications And References For Further Study.
Practice Candidacy Exam
This document provides an overview of shotgun DNA mapping using single molecule DNA analysis techniques. It discusses digesting genomic DNA into random fragments, constructing DNA tethers for optical tweezers unzipping, simulating unzipping profiles to create a library for comparison to experimental data, and potential applications for studying chromatin structure, transcription, and telomeres. The goal is to map DNA sequences to their genomic locations in order to better understand protein-DNA interactions and gene expression at the single molecule level.
D nase, dms, microarray
This document discusses three techniques for analyzing DNA-protein interactions: DNase footprinting, DMS footprinting, and microarrays. DNase footprinting uses DNase I enzyme to cleave DNA, which is then resolved by gel electrophoresis to identify protein-protected regions. DMS footprinting uses dimethyl sulfate to modify DNA bases, and reverse transcription to detect modifications that indicate protein binding sites. Microarrays immobilize DNA probes on chips to detect target sequences through fluorescent hybridization, allowing analysis of gene expression profiles. These techniques provide insights into protein-DNA binding and structural changes in nucleic acids.
SNP Detection Methods and applications
complete Single Nucleotide Polymorphiitsm Detection methods with Advance techniques with its applications
Single nucleotide polymorphisms are single base variations between genomes within a species.
There are at least 10 million polymorphic sites in the human genome.
SNPs can distinguish individuals from one another
Denaturing Gradient Gel Electrophoresis
Chemical Cleavage Of Mismatch
Single-stranded Conformation Polymorphism (SSCP)
MutS Protein-binding Assays
Mismatch Repair Detection (MRD)
Heteroduplex Analysis (HA)
Denaturing High Performance Liquid Chromatography (DHPLC)
UNG-Mediated T-Sequencing
RNA-Mediated Finger printing with MALDI MS Detection
Sequencing by Hybridization
Direct DNA Sequencing
Single-feature polymorphism (SFP)
Invader probe
Allele-specific oligonucleotide probes
PCR-based methods
Allele specific primers
Sequence Polymorphism-Derived (SPD) markers
Targeting induced local lesions in genomes (TILLinG)
Minisequencing primers
Allele-specific ligation probes
Molecular Markers
Dr. S. MANIKANDAN, M.Sc., Ph.D
Lecturer in Botany
Thiruvalluvar University Model Constituent College,
Tittagudi 606 106, Tamil Nadu, India.
Email id: drgsmanikandan@gmail.com
Genomica - Microarreglos de DNA
The document discusses various applications and techniques of DNA microarrays, including summarizing key points about Affymetrix GeneChips, spotted microarrays, experimental design, data analysis, and several case studies on various topics like ovarian cancer, Sjogren's syndrome, wine yeast genomics, and norovirus genotyping. Microarrays allow analysis of gene expression patterns and copy number variations across genomes through comparative hybridization experiments. The document provides an overview of microarray technology and applications in genomic and biomedical research.
Lecture 28
The document summarizes key concepts in molecular genetics including DNA repair mechanisms using nucleases and DNA polymerase, telomeres and their role, transcription which involves copying DNA into mRNA with RNA, differences between RNA and DNA like the sugar and nucleotide uracil, codons and their role in specifying amino acids, and Marshall Nirenberg's experiments concluding that DNA is a universal genetic code and mRNA acts as a template from its 5' to 3' end.
Tools used in molecular biology
This document provides information about genetic polymorphisms and methods used to detect single nucleotide polymorphisms (SNPs), including DNA isolation, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and gel electrophoresis. It defines a polymorphism as a harmless DNA variation between individuals and SNPs as single base mutations that are the most common source of genetic variation. The document outlines the steps of PCR including melting, annealing, and extension cycles to amplify DNA, as well as how restriction enzymes are used to digest PCR products into different fragments depending on sequences, which can then be separated by size via gel electrophoresis to detect SNPs.
Ppt snp detection
This document summarizes methods for detecting single nucleotide polymorphisms (SNPs). It discusses two main approaches: detecting unknown SNPs and detecting known SNPs. For unknown SNPs, global methods like restriction fragment length polymorphisms and targeted methods like denaturing gradient gel electrophoresis are used to scan DNA for new polymorphisms. For known SNPs, techniques like hybridization, primer extension, ligation, and mass spectrometry can be applied to screen individuals. Direct DNA sequencing is also discussed as the gold standard method, though historically it was labor-intensive. The document provides details on the principles and advantages/limitations of various SNP detection techniques.
Dna footprinting
DNA footprinting is a technique to study how proteins interact with DNA. It involves treating DNA with nucleases or chemicals that cut DNA, but are blocked from cutting where a protein is bound. This leaves a "footprint" of protected DNA that reveals the protein's binding site. The technique was developed in 1978 and can detect even transient protein-DNA interactions to help understand transcriptional control within cells.
Nanopore sequencing
Nanopore sequencing is a fourth generation DNA sequencing technique that involves monitoring changes in electric current as DNA molecules pass through nanopores. There are two main types of nanopores: biological nanopores made of protein complexes like alpha-hemolysin, and solid state nanopores made in thin silicon nitride membranes. Nanopore sequencing has advantages of being label-free, producing long reads at high throughput with low material requirements, but challenges include slowing DNA translocation and reducing noise. Potential applications are in single molecule sensing for analysis of biomolecules.
Mapping by pcr
The document discusses how polymerase chain reaction (PCR) has revolutionized the creation of molecular maps. PCR allows for the amplification of specific DNA sequences, enabling the use of DNA-based markers for mapping genomes. These PCR-based markers include RAPDs, AFLPs, and STSs. Genetic maps show the relative order and distances between markers, while physical maps provide actual base pair distances. Molecular mapping has advanced our understanding of genome structure and function and aided research into genetic diseases.
Snp by wajahahat ali
This document discusses single nucleotide polymorphisms (SNPs). It defines SNPs as single base changes in DNA sequences that occur in at least 0.1% of a population. SNPs are usually the result of errors in DNA replication or damage. They can be found in both coding and non-coding regions, and in coding regions they can alter protein structure. Applications of SNP analysis include gene discovery, disease association studies, and drug development. The document also describes several methods for SNP detection and identification, including PCR-based and non gel-based genotyping techniques as well as direct DNA sequencing.
Microarray (DNA and SNP microarray)
DNA microarrays, also known as DNA chips, allow researchers to analyze large numbers of DNA sequences simultaneously. They contain thousands of unique DNA probes immobilized on a solid surface in an organized grid pattern. Unknown DNA samples are labeled with fluorescent dyes and hybridized to the probes on the chip. The bound DNA is then detected using lasers and analyzed by a computer to determine which genes are present or expressed in the sample. There are two main types of DNA chips - cDNA-based chips containing amplified cDNA probes, and oligonucleotide-based chips containing short, synthesized DNA sequences. DNA microarrays have many applications including gene expression profiling, comparative genomics, disease diagnosis, and drug discovery.
Single Nucleotide Polymorphism
Single nucleotide polymorphisms (SNPs) are genetic variations that occur when a single nucleotide differs between members of a species. SNPs are very common, occurring around every 300 bases, and are useful for studying genetic contributions to diseases and drug responses. Researchers can identify SNPs by comparing genome sequences from many individuals and generating SNP maps and profiles that show common genetic variations.
Nanoparticle corona study -
The document discusses the protein corona that forms around nanoparticles when introduced to a biological environment. The hard corona consists of proteins that firmly attach to the nanoparticle surface. Several factors influence the composition of the corona, including the nanoparticle's hydrophobicity, size, charge, sequence, topology, and surrounding environment. The protein corona determines how cells interact with and respond to the nanoparticle. Nanoparticles show potential for applications such as disease treatment, imaging, and drug delivery, but also must be evaluated for their cytotoxicity and biodistribution within the human body.
Mumps virus
The document discusses mumps virus, which causes mumps disease. It is classified in the Paramyxoviridae family. Mumps virus has a non-segmented, negative-stranded RNA genome containing 7 transcription units that encode 8 proteins. The study analyzed the lipid and protein profiles of mumps virus grown in two cell lines, CEF and Vero, using mass spectrometry. In CEF cells, 4 viral proteins were identified (HN, NP, M, P), while in Vero cells 6 were found (HN, NP, M, P, L, V). The lipid profile depended on the host cell line, with more information obtained from Vero cells. Common proteins in both cell lines were
Single nucleotide polymorphism (sn ps)
Single nucleotide polymorphisms (SNPs) are variations in single nucleotides that occur at specific positions in the genome. For example, the base C may appear in most individuals at a specific position, but in some individuals the position is occupied by base A, representing a SNP. SNPs can be classified as synonymous or nonsynonymous depending on whether they change the amino acid sequence. Common human diseases like sickle cell anemia and cystic fibrosis result from SNPs. SNPs can be identified and analyzed using methods like DNA sequencing, mass spectrometry, and primer extension.
Maxam and gibert dna sequencing
Maxam and gibert dna sequencing notes for bachelor's and master's in Microbiology and related fields.
DNA repair mechanism
DNA repair is a crucial process that cells use to fix mutations in DNA. The cell's DNA repair mechanisms continuously scan the genome to identify and repair mutations before they can harm the cell. There are several pathways for DNA repair, including direct repair, excision repair, mismatch repair, recombinational repair, and SOS repair. Direct repair directly reverts damaged nucleotides to their original structures, but can only repair a small number of damage types, like UV radiation-induced damage repaired by photoreactivation. Excision repair removes damaged nucleotides and uses the undamaged strand as a template for resynthesis. Mismatch repair fixes errors from DNA replication by recognizing mismatches and excising the incorrect segment. [END SUMMARY]
Northern blotting
Northern blotting is a technique used to detect RNA in a sample. It involves isolating RNA from cells, separating RNA fragments by size using gel electrophoresis, transferring the RNA fragments to a membrane, then using a labeled probe to detect specific RNA sequences through hybridization and visualization. The northern blot allows researchers to study gene expression at the mRNA level and determine which genes are expressed under different conditions or in different tissues.
DNA footprinting
DNA footprinting is a technique used to identify protein binding regions on DNA. It involves treating DNA with nucleases like DNase I, which will degrade the DNA except for regions bound by proteins. These protected regions, called footprints, can identify transcription factor binding sites that regulate gene expression. The technique was originally developed in 1978 to study the binding specificity of the lac repressor protein, and it provides information on DNA-protein interactions and transcriptional regulation.
Protein corona associated with nanoparticles
The document discusses protein coronas that form around nanoparticles when introduced into biological fluids and how they impact physiological response. It summarizes that nanoparticles interact with proteins to form complexes with unique identities compared to the original nanoparticle. These complexes determine responses like uptake, circulation and toxicity. The document then examines several studies that show how nanoparticle properties like size and surface chemistry influence protein adsorption and subsequently impact biological response. It also reviews techniques for characterizing and experimentally investigating protein coronas and their effects.
Identification Technique for Gene
This document discusses three biotechnology techniques: DNA microarray, gene sequencing, and SDS-PAGE. It provides details on the principles, methods, and steps for each technique. DNA microarray allows analysis of gene expression for thousands of genes using DNA spots on a solid surface. Gene sequencing determines the order of genes along a chromosome using methods like directed sequencing and shotgun libraries. SDS-PAGE separates molecules by size using polyacrylamide gel and SDS to neutralize protein charge.
Gene expression introduction
Microarray as one of recent biomedical technologies produce high dimensional data. This makes statistical analysis become challenging. I presented an overview of microarray analysis specifically in the use of gene expression profiling in a discussion.
SNPs analysis methods
This document discusses various methods for single nucleotide polymorphism (SNP) analysis, including their principles, advantages, and disadvantages. It describes SNP genotyping techniques like RFLP-PCR, TaqMan assays, microarrays, Sanger sequencing, SNaPshot, and next-generation sequencing. The key aspects are accuracy, throughput, cost, and ability to detect both known and unknown SNPs. The document emphasizes choosing methods based on required information extraction and cost effectiveness.
Single Nucleotide Polymorphism
The change in one nucleotide in a genome is known as single nucleotide polymorphism. There are assorted types of SNPs. SNPs can be detected by several analytical techniques i.e. DNA sequencing, microchip, HPLC and oligonucleotide ligation reaction.
Segojoven nº5
Este documento resume las actividades y campañas realizadas por Juventudes Socialistas de Segovia entre octubre de 2013 y enero de 2014. Incluye un cinefórum sobre violencia machista en los jóvenes, una mesa redonda sobre homofobia, campañas sobre VIH, violencia machista y despoblación, apoyo a la huelga educativa del 24 de octubre contra la LOMCE y a la manifestación contra la reforma de la ley del aborto. También describe campañas para defender las becas Erasmus y denunciar el riesgo de
Primeira lista sql
O documento fornece instruções para um exercício de banco de dados SQL, pedindo para (1) criar um banco de dados chamado LOJA e (2) criar as tabelas Vendedor, Produto e Venda dentro desse banco de dados.
More Related Content
What's hot
Tools used in molecular biology
This document provides information about genetic polymorphisms and methods used to detect single nucleotide polymorphisms (SNPs), including DNA isolation, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and gel electrophoresis. It defines a polymorphism as a harmless DNA variation between individuals and SNPs as single base mutations that are the most common source of genetic variation. The document outlines the steps of PCR including melting, annealing, and extension cycles to amplify DNA, as well as how restriction enzymes are used to digest PCR products into different fragments depending on sequences, which can then be separated by size via gel electrophoresis to detect SNPs.
Ppt snp detection
This document summarizes methods for detecting single nucleotide polymorphisms (SNPs). It discusses two main approaches: detecting unknown SNPs and detecting known SNPs. For unknown SNPs, global methods like restriction fragment length polymorphisms and targeted methods like denaturing gradient gel electrophoresis are used to scan DNA for new polymorphisms. For known SNPs, techniques like hybridization, primer extension, ligation, and mass spectrometry can be applied to screen individuals. Direct DNA sequencing is also discussed as the gold standard method, though historically it was labor-intensive. The document provides details on the principles and advantages/limitations of various SNP detection techniques.
Dna footprinting
DNA footprinting is a technique to study how proteins interact with DNA. It involves treating DNA with nucleases or chemicals that cut DNA, but are blocked from cutting where a protein is bound. This leaves a "footprint" of protected DNA that reveals the protein's binding site. The technique was developed in 1978 and can detect even transient protein-DNA interactions to help understand transcriptional control within cells.
Nanopore sequencing
Nanopore sequencing is a fourth generation DNA sequencing technique that involves monitoring changes in electric current as DNA molecules pass through nanopores. There are two main types of nanopores: biological nanopores made of protein complexes like alpha-hemolysin, and solid state nanopores made in thin silicon nitride membranes. Nanopore sequencing has advantages of being label-free, producing long reads at high throughput with low material requirements, but challenges include slowing DNA translocation and reducing noise. Potential applications are in single molecule sensing for analysis of biomolecules.
Mapping by pcr
The document discusses how polymerase chain reaction (PCR) has revolutionized the creation of molecular maps. PCR allows for the amplification of specific DNA sequences, enabling the use of DNA-based markers for mapping genomes. These PCR-based markers include RAPDs, AFLPs, and STSs. Genetic maps show the relative order and distances between markers, while physical maps provide actual base pair distances. Molecular mapping has advanced our understanding of genome structure and function and aided research into genetic diseases.
Snp by wajahahat ali
This document discusses single nucleotide polymorphisms (SNPs). It defines SNPs as single base changes in DNA sequences that occur in at least 0.1% of a population. SNPs are usually the result of errors in DNA replication or damage. They can be found in both coding and non-coding regions, and in coding regions they can alter protein structure. Applications of SNP analysis include gene discovery, disease association studies, and drug development. The document also describes several methods for SNP detection and identification, including PCR-based and non gel-based genotyping techniques as well as direct DNA sequencing.
Microarray (DNA and SNP microarray)
DNA microarrays, also known as DNA chips, allow researchers to analyze large numbers of DNA sequences simultaneously. They contain thousands of unique DNA probes immobilized on a solid surface in an organized grid pattern. Unknown DNA samples are labeled with fluorescent dyes and hybridized to the probes on the chip. The bound DNA is then detected using lasers and analyzed by a computer to determine which genes are present or expressed in the sample. There are two main types of DNA chips - cDNA-based chips containing amplified cDNA probes, and oligonucleotide-based chips containing short, synthesized DNA sequences. DNA microarrays have many applications including gene expression profiling, comparative genomics, disease diagnosis, and drug discovery.
Single Nucleotide Polymorphism
Single nucleotide polymorphisms (SNPs) are genetic variations that occur when a single nucleotide differs between members of a species. SNPs are very common, occurring around every 300 bases, and are useful for studying genetic contributions to diseases and drug responses. Researchers can identify SNPs by comparing genome sequences from many individuals and generating SNP maps and profiles that show common genetic variations.
Nanoparticle corona study -
The document discusses the protein corona that forms around nanoparticles when introduced to a biological environment. The hard corona consists of proteins that firmly attach to the nanoparticle surface. Several factors influence the composition of the corona, including the nanoparticle's hydrophobicity, size, charge, sequence, topology, and surrounding environment. The protein corona determines how cells interact with and respond to the nanoparticle. Nanoparticles show potential for applications such as disease treatment, imaging, and drug delivery, but also must be evaluated for their cytotoxicity and biodistribution within the human body.
Mumps virus
The document discusses mumps virus, which causes mumps disease. It is classified in the Paramyxoviridae family. Mumps virus has a non-segmented, negative-stranded RNA genome containing 7 transcription units that encode 8 proteins. The study analyzed the lipid and protein profiles of mumps virus grown in two cell lines, CEF and Vero, using mass spectrometry. In CEF cells, 4 viral proteins were identified (HN, NP, M, P), while in Vero cells 6 were found (HN, NP, M, P, L, V). The lipid profile depended on the host cell line, with more information obtained from Vero cells. Common proteins in both cell lines were
Single nucleotide polymorphism (sn ps)
Single nucleotide polymorphisms (SNPs) are variations in single nucleotides that occur at specific positions in the genome. For example, the base C may appear in most individuals at a specific position, but in some individuals the position is occupied by base A, representing a SNP. SNPs can be classified as synonymous or nonsynonymous depending on whether they change the amino acid sequence. Common human diseases like sickle cell anemia and cystic fibrosis result from SNPs. SNPs can be identified and analyzed using methods like DNA sequencing, mass spectrometry, and primer extension.
Maxam and gibert dna sequencing
Maxam and gibert dna sequencing notes for bachelor's and master's in Microbiology and related fields.
DNA repair mechanism
DNA repair is a crucial process that cells use to fix mutations in DNA. The cell's DNA repair mechanisms continuously scan the genome to identify and repair mutations before they can harm the cell. There are several pathways for DNA repair, including direct repair, excision repair, mismatch repair, recombinational repair, and SOS repair. Direct repair directly reverts damaged nucleotides to their original structures, but can only repair a small number of damage types, like UV radiation-induced damage repaired by photoreactivation. Excision repair removes damaged nucleotides and uses the undamaged strand as a template for resynthesis. Mismatch repair fixes errors from DNA replication by recognizing mismatches and excising the incorrect segment. [END SUMMARY]
Northern blotting
Northern blotting is a technique used to detect RNA in a sample. It involves isolating RNA from cells, separating RNA fragments by size using gel electrophoresis, transferring the RNA fragments to a membrane, then using a labeled probe to detect specific RNA sequences through hybridization and visualization. The northern blot allows researchers to study gene expression at the mRNA level and determine which genes are expressed under different conditions or in different tissues.
DNA footprinting
DNA footprinting is a technique used to identify protein binding regions on DNA. It involves treating DNA with nucleases like DNase I, which will degrade the DNA except for regions bound by proteins. These protected regions, called footprints, can identify transcription factor binding sites that regulate gene expression. The technique was originally developed in 1978 to study the binding specificity of the lac repressor protein, and it provides information on DNA-protein interactions and transcriptional regulation.
Protein corona associated with nanoparticles
The document discusses protein coronas that form around nanoparticles when introduced into biological fluids and how they impact physiological response. It summarizes that nanoparticles interact with proteins to form complexes with unique identities compared to the original nanoparticle. These complexes determine responses like uptake, circulation and toxicity. The document then examines several studies that show how nanoparticle properties like size and surface chemistry influence protein adsorption and subsequently impact biological response. It also reviews techniques for characterizing and experimentally investigating protein coronas and their effects.
Identification Technique for Gene
This document discusses three biotechnology techniques: DNA microarray, gene sequencing, and SDS-PAGE. It provides details on the principles, methods, and steps for each technique. DNA microarray allows analysis of gene expression for thousands of genes using DNA spots on a solid surface. Gene sequencing determines the order of genes along a chromosome using methods like directed sequencing and shotgun libraries. SDS-PAGE separates molecules by size using polyacrylamide gel and SDS to neutralize protein charge.
Gene expression introduction
Microarray as one of recent biomedical technologies produce high dimensional data. This makes statistical analysis become challenging. I presented an overview of microarray analysis specifically in the use of gene expression profiling in a discussion.
SNPs analysis methods
This document discusses various methods for single nucleotide polymorphism (SNP) analysis, including their principles, advantages, and disadvantages. It describes SNP genotyping techniques like RFLP-PCR, TaqMan assays, microarrays, Sanger sequencing, SNaPshot, and next-generation sequencing. The key aspects are accuracy, throughput, cost, and ability to detect both known and unknown SNPs. The document emphasizes choosing methods based on required information extraction and cost effectiveness.
Single Nucleotide Polymorphism
The change in one nucleotide in a genome is known as single nucleotide polymorphism. There are assorted types of SNPs. SNPs can be detected by several analytical techniques i.e. DNA sequencing, microchip, HPLC and oligonucleotide ligation reaction.
What's hot (20)
Viewers also liked
Segojoven nº5
Este documento resume las actividades y campañas realizadas por Juventudes Socialistas de Segovia entre octubre de 2013 y enero de 2014. Incluye un cinefórum sobre violencia machista en los jóvenes, una mesa redonda sobre homofobia, campañas sobre VIH, violencia machista y despoblación, apoyo a la huelga educativa del 24 de octubre contra la LOMCE y a la manifestación contra la reforma de la ley del aborto. También describe campañas para defender las becas Erasmus y denunciar el riesgo de
Primeira lista sql
O documento fornece instruções para um exercício de banco de dados SQL, pedindo para (1) criar um banco de dados chamado LOJA e (2) criar as tabelas Vendedor, Produto e Venda dentro desse banco de dados.
Practica n° 4
The document contains a table with 50 rows listing Peruvian Sol (S/. ) amounts, percentages, and sums across two columns and a total. Each row increases the Sol amount by S/.1 and the percentage by 300%, with the time listed in the left column increasing hourly from 12:00am to 12:00am the next day. The sums and totals in each row increase accordingly as the hour and amounts progress throughout the table.
Tics mapas conseptuales
Las TIC son fundamentales en la sociedad moderna y la educación debe incorporarlas para preparar a los estudiantes para esta realidad. La educación debe tener en cuenta las TIC y enseñar sobre tecnología de información y comunicación para que los ciudadanos estén preparados para una sociedad basada en las TIC.
Zirkunferentzia
El documento presenta las propiedades de las circunferencias y los ángulos asociados. Define elementos como radio, diámetro, cuerda y arco. Explica las relaciones entre circunferencias tangentes, secantes y concéntricas. Incluye teoremas como los de Pitágoras y Poncelet y ejercicios de aplicación.
Cables17
Este documento describe los diferentes tipos de cables y conectores necesarios para conectar los componentes de un PC, incluyendo cables de datos IDE de 40 y 80 hilos, cables SATA, SCSI, USB, Firewire, PS/2, UTP, de video VGA y DVI, de audio mini jack, y conectores eléctricos ATX y Molex para suministrar energía a la placa base y dispositivos.
Periodontics instruments.
This document describes various periodontal instruments used in examining, cleaning, and surgically treating the gums and supporting structures of the teeth. It provides details on the uses, designs, and techniques associated with probes, scalers, curettes, and other instruments. Periodontal probes are used to measure pocket depths and assess bone loss, while scalers, curettes, files and other instruments remove calculus, root surface irregularities, and diseased tissue either above or below the gumline. The document also mentions specialty instruments like endoscopes, periotreivers, and sonic/ultrasonic devices that aid in visualization and cleaning.
Viewers also liked (8)
Similar to Seminario BM
Blotting techniques
The document discusses the history and techniques of blotting methods including Southern blotting, Northern blotting, and Western blotting. Southern blotting was developed in 1975 by Edwin Southern to detect DNA fragments transferred to membranes. Northern blotting was developed in 1979 to detect specific RNA sequences. Western blotting was also developed in 1979 to detect newly encoded proteins. These blotting techniques involve separating biomolecules by electrophoresis, transferring them to membranes, and using probes or antibodies to detect specific sequences or proteins.
Principle and applications of blotting techniques
The document discusses various blotting techniques used in molecular biology including Northern blotting, Southern blotting, dot blotting, colony hybridization, and plaque hybridization.
Northern blotting involves separating RNA samples by size, transferring them to a membrane, and using a probe to detect specific sequences. Southern blotting is used to detect specific DNA sequences by separating DNA fragments, transferring them to a membrane, and using probes. Dot blotting simplifies the detection of proteins by applying samples directly to a membrane. Colony hybridization screens bacterial colonies for genes of interest by transferring DNA to a membrane and using probes. Plaque hybridization identifies recombinant phages using a similar process to colony hybridization.
ATM-Deficient Colorectal Cancer Cells Are Sensitive to the PARP Inhibitor Ola...
This document discusses research into using PARP inhibitors like olaparib to treat colorectal cancer (CRC). The researchers found that CRC cell lines with mutations in the ATM gene, which is involved in DNA damage repair, were more sensitive to olaparib than cell lines without ATM mutations. They showed that inhibiting both ATM and PARP together was more effective at killing CRC cells than inhibiting either pathway alone. The researchers concluded that cancers involving ATM mutations, including CRC, may be good candidates for PARP inhibitor treatment. Testing patients' tumors for ATM mutations could help guide treatment decisions.
Seminario biologia
Recurrent somatic DICER1 mutations were found in nonepithelial ovarian cancers. These mutations occurred in codons encoding metal binding sites within the RNase IIIb catalytic centers of DICER1. The mutations were restricted to these sites and did not completely abolish DICER1 function, but instead disrupted microRNA processing in a cell-type specific manner, leading to oncogenesis in certain cell types like ovarian epithelial cells. Validation studies found these DICER1 mutations in a high percentage of additional nonepithelial ovarian tumor samples.
Principle and application of blotting techniques
This document discusses various blotting techniques used in molecular biology including Northern blotting, Southern blotting, dot blotting, colony hybridization, and plaque hybridization. Northern blotting is used to detect RNA, Southern blotting detects specific DNA sequences, dot blotting detects proteins without separation, colony hybridization screens bacterial colonies for desired genes, and plaque hybridization identifies recombinant phages. These techniques allow for detection and analysis of nucleic acids and proteins to study gene expression, mutations, genetic diseases, and more.
Seminario biomol.pdf
This document discusses research into inhibiting prostate cancer cells. It examines the effects of Remodelin, an inhibitor of NAT10, on prostate cancer cells and potential mechanisms. Methods used include hematoxylin and eosin staining, western blotting, immunofluorescence, and TUNEL assays. Results show Remodelin treatment inhibits DNA replication in cancer cells by influencing chromatin acetylation status. The discussion suggests molecular biology plays an important role in solving prostate cancer by revealing DNA replication in prostate cancer cells can be inhibited.
Ribosomal rna sequencing
Ribosomes are complex structures found in all living cells which functions in protein synthesis machinery. Basically ribosome’s consists of two subunits, each of which is composed of protein and a type of RNA, known as ribosomal RNA (rRNA). Prokaryotic ribosomes consist of 30S subunit (small sub unit) and 50S subunit (large sub unit) which together make up the complete 70S ribosome, where S stands for Svedberg unit non-SI unit for sedimentation rate. 30S subunit is composed of 16S ribosomal RNA and 21 polynucleotide chains while 50S subunit is composed of two rRNA species, the 5S and 23S rRNAs. The presence of hyper variable regions in the 16S rRNA gene provides a species specific signature sequence which is useful for bacterial identification process. 16S Ribosomal RNA sequencing is widely used in microbiology studies to identify the diversities in prokaryotic organisms as well as other organisms and thereby studying the phylogenetic relationships between them. The advantages of using ribosomal RNA in molecular techniques are as follows
Ribosomes and ribosomal RNA are present in all cells.
RNA genes are highly conserved in nature.
Culturing of microbial cells is absent in the sequencing techniques.
Western Blotting Of Camkii Β And T 287
1. Tomato production is affected by various bacterial, fungal and viral diseases which can cause considerable yield losses.
2. One of the most devastating diseases is tomato leaf curl disease (ToLCD), caused by geminiviruses, which is increasing worldwide and poses a major constraint to tomato production in India.
3. ToLCD causes serious yield losses according to studies from the 1940s and more recently. Effective management strategies are needed to control this and other diseases threatening tomato production.
Northern blotting
This presentation is all about Northern blotting technique ..which is a biotechnological process used in cell and molecular biology studies.
Nucleic acid hybridization
Nucleic acid hybridization is a technique used to identify specific DNA sequences. It involves denaturing DNA or RNA samples and probes, followed by annealing of the probes to complementary sequences. There are two main types: Southern blotting separates DNA fragments by gel electrophoresis before hybridization with probes, while Northern blotting separates RNA this way. Both techniques allow detection of specific sequences through the use of labeled probes.
Method of detection of food borne pathogen(methods).docx
PCR and RT-PCR are commonly used molecular techniques for detecting foodborne pathogens through amplification of pathogen DNA or RNA. Multiplex PCR (mPCR) allows simultaneous detection of multiple pathogens. Real-time PCR monitors amplification in real-time without gel electrophoresis. Other methods like LAMP, NASBA, and microarrays provide isothermal amplification or detect multiple targets but require different primers or probes. Optical and electrochemical biosensors detect binding through surface plasmon resonance or changes in electrical signals. Mass-based sensors measure added mass through resonant frequency changes of piezoelectric crystals. ELISA is a common immunological technique that sandwiches the target antigen between immobilized and enzyme-conjugated antibodies for colorimetric detection.
Complete the table WHAT QUESTION DOES EACH ANSWER... WHAT IS THE G.pdf
Complete the table
WHAT QUESTION DOES EACH ANSWER... WHAT IS THE GOAL FOR EACH...
Methods
Definition
Question that answers
Draw it
RNA seq
RNA seq or RNA sequencing is the examination of the RNA in a sample using next-generation
sequencing by analyzing the cellular transcriptome of RNA-encoded gene expression patterns.
MeDIP-Seq
MeDIP-Seq is an abbreviation that stands for Methylated DNA Immunoprecipitation
Sequencing. It is a technique used to examine patterns of DNA methylation in the genome.
Utilizing an antibody that binds specifically to methylated DNA, the DNA is then isolated and
sequenced. This enables researchers to identify methylated regions of the genome and investigate
their function. MeDIP-Seq can be used to answer queries such as how DNA methylation
influences gene expression, how it varies between cell types or tissues, and how it responds to
the environment.
HM450K
HM450K stands for HumanMethylation450 BeadChip by Illumina. It is a microarray-based
technique used to examine patterns of DNA methylation in the human genome. The device
contains probes that target over 450,000 CpG sites throughout the genome, enabling researchers
to identify methylated or unmethylated regions of the genome. HM450K can be used to answer
such queries as how DNA methylation patterns differ between healthy and diseased tissues, how
they change over time, and how they are influenced by the environment.
Mass Spec
The technique of mass spectrometry (MS) is used to analyze molecules based on their mass-to-
charge ratio. Utilized frequently in proteomics research to identify and quantify proteins in
biological samples. The method entails ionizing the sample molecules, separating them
according to their mass-to-charge ratio, and detecting them with a mass spectrometer. MS can be
used to determine which proteins are present in a biological sample, how much of each is
present, and how they interact.
EMSA
Electrophoretic Mobility Shift Assay stands for EMSA. It is a technique utilized to investigate
protein-DNA interactions. A labeled DNA probe is incubated with a protein extract from cells or
tissues, followed by electrophoresis on a polyacrylamide gel. If the protein binds to the DNA
probe, its electrophoretic mobility on the gel will change. EMSA can be used to determine which
proteins bind to a particular DNA sequence, as well as to investigate the kinetics and specificity
of protein-DNA interactions. This technique is frequently employed in the field of transcriptional
regulation, where it can be used to investigate how transcription factors bind to DNA and
regulate gene expression. Other protein-DNA interactions, such as those involved in DNA repair
or replication, can also be studied using EMSA. Overall, EMSA is an effective method for
investigating protein-DNA interactions and their function in cellular processes.
MNAse-Seq
MNase-Seq is an acronym that stands for Micrococcal Nuclease Sequencing. It is a technique
used to examine the structure and acce.
GEL ELECTROPHORESIS WITH BLOT TECHNIQUES.pptx
This document provides an overview of gel electrophoresis and blotting techniques. It describes how gel electrophoresis uses a gel matrix to separate macromolecules like DNA, RNA, and proteins based on size and charge when an electric current is applied. The document then discusses different types of gel electrophoresis including agarose gel electrophoresis, polyacrylamide gel electrophoresis, and starch gel electrophoresis. It also summarizes common blotting techniques like Southern blotting for detecting DNA, Northern blotting for detecting RNA, and Western blotting for detecting proteins. The document provides details on the basic principles and procedures for each technique.
BIOTECHNOLOGY PPT.pptx
Genomics is the study of genomes through DNA sequencing and analysis. There are several types of genomics including structural, functional, comparative, epigenomics, and metagenomics. DNA sequencing methods have advanced from Sanger sequencing to next-generation sequencing using high-throughput technologies. Genome sequencing has provided insights into disease, evolution, and applications in medicine, agriculture, and environmental science.
Prabhakar singh ii sem-paper v-pcr
Prabhakar singh ii sem-paper v-pcrDepartment of Biochemistry, Veer Bahadur Singh Purvanchal Univarsity, Jaunpur
Polymerase chain reaction (PCR) is a technique used to amplify a specific DNA sequence, allowing for millions of copies to be generated. It involves repeated cycles of heating and cooling of the DNA sample to separate the DNA strands, followed by using primers and a DNA polymerase to selectively replicate the target sequence. Each cycle doubles the amount of target DNA. After many cycles, sufficient copies of the sequence are produced for analysis. PCR has numerous applications, including disease diagnosis, DNA sequencing, evolutionary studies, forensics, and more. It is a fundamental technique in molecular biology labs.Lydia Yeshitla, Research Scholar at the Neurobiology Section of UCSD
1) The document describes an experiment cloning a pH-sensitive fluorescent protein (pHRed) onto the GLUA1 AMPA receptor subunit to track intracellular trafficking and degradation of AMPA receptors by lysosomes.
2) Restriction enzymes (AGE1 and BSRG1) were used to cut the DNA in order to ligate pHRed onto GLUA1 using PCR. This would allow detection of AMPA receptors in the acidic lysosome lumen.
3) Bacteria were transformed with the ligated pHRed-GluA1 DNA. Colonies were selected and the DNA was sequenced to validate that the cloning procedure was done correctly.
Pranamee Sarma Assignment Technologies A20263866
1. The document discusses using laser capture microdissection to isolate DNA from seminiferous tubules containing mature spermatids from testicular tissue of normal and Menkes-affected males. This would allow isolation of pure cell populations for further DNA analysis.
2. Reverse transcriptase PCR is described to transcribe mRNA from the isolated cells into cDNA, which can then be amplified and sequenced to identify any mutations between normal and diseased samples.
3. Quantitative PCR is discussed to couple with reverse transcriptase PCR to fluorescently label and quantify the cDNA, enabling target quantification and analysis of gene expression changes for Menkes disease therapy development.
Recombinant DNA
Restriction endonucleases are enzymes that cut DNA at specific sequences. They have been used to map DNA by cutting it into fragments of different sizes that can be separated by gel electrophoresis. More than 3000 restriction endonucleases have been isolated from bacteria and are useful for applications such as cloning DNA fragments into vectors like plasmids. The ability to cut and paste DNA fragments using restriction enzymes and recombinant DNA technology has enabled scientists to study genes and their functions.
Blotting technique including Southern , Northern and Western blotting
he given ppt contains all the blotting techniques which is being studied by students in Biotechnology related subject and this PPT contais all blotting techniques in a very elaborative concise manner includes procedure principle application etc so which itwould help any bio student to take proper knowledge in this topic. I hope you will enjoy the content of the topic and would be able to grasp the topic properly
Tools & techniques (Molecular & Biochemical to Study Physiological Processes ...
Tools & techniques (Molecular & Biochemical to Study Physiological Processes & to Screen & Asses Stress Response in Plants)
Similar to Seminario BM (20)
ATM-Deficient Colorectal Cancer Cells Are Sensitive to the PARP Inhibitor Ola...
ATM-Deficient Colorectal Cancer Cells Are Sensitive to the PARP Inhibitor Ola...
Method of detection of food borne pathogen(methods).docx
Method of detection of food borne pathogen(methods).docx
Complete the table WHAT QUESTION DOES EACH ANSWER... WHAT IS THE G.pdf
Complete the table WHAT QUESTION DOES EACH ANSWER... WHAT IS THE G.pdf
Lydia Yeshitla, Research Scholar at the Neurobiology Section of UCSD
Lydia Yeshitla, Research Scholar at the Neurobiology Section of UCSD
Blotting technique including Southern , Northern and Western blotting
Blotting technique including Southern , Northern and Western blotting
Tools & techniques (Molecular & Biochemical to Study Physiological Processes ...
Tools & techniques (Molecular & Biochemical to Study Physiological Processes ...
Recently uploaded
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Sidekick Solutions uses Bonterra Impact Management (fka Social Solutions Apricot) and automation solutions to integrate data for business workflows.
We believe integration and automation are essential to user experience and the promise of efficient work through technology. Automation is the critical ingredient to realizing that full vision. We develop integration products and services for Bonterra Case Management software to support the deployment of automations for a variety of use cases.
This video focuses on integration of Salesforce with Bonterra Impact Management.
Interested in deploying an integration with Salesforce for Bonterra Impact Management? Contact us at sales@sidekicksolutionsllc.com to discuss next steps.
Presentation of the OECD Artificial Intelligence Review of Germany
Consult the full report at https://www.oecd.org/digital/oecd-artificial-intelligence-review-of-germany-609808d6-en.htm
Taking AI to the Next Level in Manufacturing.pdf
Read Taking AI to the Next Level in Manufacturing to gain insights on AI adoption in the manufacturing industry, such as:
1. How quickly AI is being implemented in manufacturing.
2. Which barriers stand in the way of AI adoption.
3. How data quality and governance form the backbone of AI.
4. Organizational processes and structures that may inhibit effective AI adoption.
6. Ideas and approaches to help build your organization's AI strategy.
Main news related to the CCS TSI 2023 (2023/1695)
An English 🇬🇧 translation of a presentation to the speech I gave about the main changes brought by CCS TSI 2023 at the biggest Czech conference on Communications and signalling systems on Railways, which was held in Clarion Hotel Olomouc from 7th to 9th November 2023 (konferenceszt.cz). Attended by around 500 participants and 200 on-line followers.
The original Czech 🇨🇿 version of the presentation can be found here: https://www.slideshare.net/slideshow/hlavni-novinky-souvisejici-s-ccs-tsi-2023-2023-1695/269688092 .
The videorecording (in Czech) from the presentation is available here: https://youtu.be/WzjJWm4IyPk?si=SImb06tuXGb30BEH .
Trusted Execution Environment for Decentralized Process Mining
Presentation of the paper "Trusted Execution Environment for Decentralized Process Mining" given during the CAiSE 2024 Conference in Cyprus on June 7, 2024.
Introduction of Cybersecurity with OSS at Code Europe 2024
I develop the Ruby programming language, RubyGems, and Bundler, which are package managers for Ruby. Today, I will introduce how to enhance the security of your application using open-source software (OSS) examples from Ruby and RubyGems.
The first topic is CVE (Common Vulnerabilities and Exposures). I have published CVEs many times. But what exactly is a CVE? I'll provide a basic understanding of CVEs and explain how to detect and handle vulnerabilities in OSS.
Next, let's discuss package managers. Package managers play a critical role in the OSS ecosystem. I'll explain how to manage library dependencies in your application.
I'll share insights into how the Ruby and RubyGems core team works to keep our ecosystem safe. By the end of this talk, you'll have a better understanding of how to safeguard your code.
TrustArc Webinar - 2024 Global Privacy Survey
How does your privacy program stack up against your peers? What challenges are privacy teams tackling and prioritizing in 2024?
In the fifth annual Global Privacy Benchmarks Survey, we asked over 1,800 global privacy professionals and business executives to share their perspectives on the current state of privacy inside and outside of their organizations. This year’s report focused on emerging areas of importance for privacy and compliance professionals, including considerations and implications of Artificial Intelligence (AI) technologies, building brand trust, and different approaches for achieving higher privacy competence scores.
See how organizational priorities and strategic approaches to data security and privacy are evolving around the globe.
This webinar will review:
- The top 10 privacy insights from the fifth annual Global Privacy Benchmarks Survey
- The top challenges for privacy leaders, practitioners, and organizations in 2024
- Key themes to consider in developing and maintaining your privacy program
June Patch Tuesday
Ivanti’s Patch Tuesday breakdown goes beyond patching your applications and brings you the intelligence and guidance needed to prioritize where to focus your attention first. Catch early analysis on our Ivanti blog, then join industry expert Chris Goettl for the Patch Tuesday Webinar Event. There we’ll do a deep dive into each of the bulletins and give guidance on the risks associated with the newly-identified vulnerabilities.
Ocean lotus Threat actors project by John Sitima 2024 (1).pptx
Ocean Lotus cyber threat actors represent a sophisticated, persistent, and politically motivated group that poses a significant risk to organizations and individuals in the Southeast Asian region. Their continuous evolution and adaptability underscore the need for robust cybersecurity measures and international cooperation to identify and mitigate the threats posed by such advanced persistent threat groups.
Nunit vs XUnit vs MSTest Differences Between These Unit Testing Frameworks.pdf
When it comes to unit testing in the .NET ecosystem, developers have a wide range of options available. Among the most popular choices are NUnit, XUnit, and MSTest. These unit testing frameworks provide essential tools and features to help ensure the quality and reliability of code. However, understanding the differences between these frameworks is crucial for selecting the most suitable one for your projects.
System Design Case Study: Building a Scalable E-Commerce Platform - Hiike
This case study explores designing a scalable e-commerce platform, covering key requirements, system components, and best practices.
Energy Efficient Video Encoding for Cloud and Edge Computing Instances
Energy Efficient Video Encoding for Cloud and Edge Computing Instances
Skybuffer SAM4U tool for SAP license adoption
Manage and optimize your license adoption and consumption with SAM4U, an SAP free customer software asset management tool.
SAM4U, an SAP complimentary software asset management tool for customers, delivers a detailed and well-structured overview of license inventory and usage with a user-friendly interface. We offer a hosted, cost-effective, and performance-optimized SAM4U setup in the Skybuffer Cloud environment. You retain ownership of the system and data, while we manage the ABAP 7.58 infrastructure, ensuring fixed Total Cost of Ownership (TCO) and exceptional services through the SAP Fiori interface.
Fueling AI with Great Data with Airbyte Webinar
This talk will focus on how to collect data from a variety of sources, leveraging this data for RAG and other GenAI use cases, and finally charting your course to productionalization.
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Discover the seamless integration of RPA (Robotic Process Automation), COMPOSER, and APM with AWS IDP enhanced with Slack notifications. Explore how these technologies converge to streamline workflows, optimize performance, and ensure secure access, all while leveraging the power of AWS IDP and real-time communication via Slack notifications.
Recently uploaded (20)
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Salesforce Integration for Bonterra Impact Management (fka Social Solutions A...
Presentation of the OECD Artificial Intelligence Review of Germany
Presentation of the OECD Artificial Intelligence Review of Germany
Trusted Execution Environment for Decentralized Process Mining
Trusted Execution Environment for Decentralized Process Mining
Introduction of Cybersecurity with OSS at Code Europe 2024
Introduction of Cybersecurity with OSS at Code Europe 2024
Nordic Marketo Engage User Group_June 13_ 2024.pptx
Nordic Marketo Engage User Group_June 13_ 2024.pptx
WeTestAthens: Postman's AI & Automation Techniques
WeTestAthens: Postman's AI & Automation Techniques
Overcoming the PLG Trap: Lessons from Canva's Head of Sales & Head of EMEA Da...
Overcoming the PLG Trap: Lessons from Canva's Head of Sales & Head of EMEA Da...
Ocean lotus Threat actors project by John Sitima 2024 (1).pptx
Ocean lotus Threat actors project by John Sitima 2024 (1).pptx
Nunit vs XUnit vs MSTest Differences Between These Unit Testing Frameworks.pdf
Nunit vs XUnit vs MSTest Differences Between These Unit Testing Frameworks.pdf
Deep Dive: AI-Powered Marketing to Get More Leads and Customers with HyperGro...
Deep Dive: AI-Powered Marketing to Get More Leads and Customers with HyperGro...
System Design Case Study: Building a Scalable E-Commerce Platform - Hiike
System Design Case Study: Building a Scalable E-Commerce Platform - Hiike
Energy Efficient Video Encoding for Cloud and Edge Computing Instances
Energy Efficient Video Encoding for Cloud and Edge Computing Instances
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Let's Integrate MuleSoft RPA, COMPOSER, APM with AWS IDP along with Slack
Seminario BM
- 1. The use of caspase inhibitors in pulsed-field gelelectrophoresis may improve the estimation ofradiation-induced DNA repair and apoptosis Josep Balart, Gemma Pueyo, Lara I de Llobet, Marta Baro, Xavi Sole, Susanna Marin, Oriol Casanovas,Ricard Mesia, Gabriel Capella. SIMON RESTREPO CAMILO RESTREPO
- 2. KEY WORDS PFGE Pulsed Field Gel Electrophoresis. CASPASES Proteins that are one of the main executors of the apoptotic process and the pro-inflammatory process too. DNA REPARATION Processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. Mechanisms APOPTOSIS Process of programmed cell death that may occur in multicellular organisms.
- 3. INTRODUCTION DNA repair in several factors which can be determined through PFGE, as are the activity mediated by caspases called programmed cell death, apoptosis. Then the objective of PFGE is determine the magnitude of radiation induced by these processes and determine that the release of DNA from cells corresponds to the intensity of the DNA fragmentation
- 4. In the PFGE technique, cells are encapsulated in agarose to form cell-plugs, thus preventing physic damage of the cells while facilitating their manipulation and placement into agarose gels where electrophoresis will take place.
- 5. GENERAL OBJECTIVE Determine the radiation generated by PFGE of DNA fragmentation and apoptosis during repair.
- 6. MATERIALES Y METODOS LINEAS CELULARES Células tumorales A431 Carcinoma Escamoso. Células NP18 Carcinoma Pancreático.
- 7. MUESTRA Ratones de 6 a 8 semanas de sexo masculino.
- 8. PROCEDIMIENTO A cada ratón se le inyecto de forma SC NP18 en su flanco para generar el tumor. 10mm Extracción/extirpación 90min incubación DMEM 30min Tripsina a 37° en agitación
- 9. ELCTROFORESIS Separación de partículas por peso y por cargas
- 10. PFGE Herramienta para examinar el ADN cromosómico que es capaz de separar fragmentos de 10 kpb hasta 10.000 kpb.
- 11. FLUORESCENCIA Es la propiedad de una sustancia de emitir luz cuando es expuesta a radiaciones
- 12. INMUNOFLUORESCENCIA La inmunofluorescencia se utiliza esencialmente en la detección de autoanticuerpos y anticuerpos contra antígenos de superficie de células y tejidos.
- 13. DIRECTA
- 14. INDIRECTA
- 15. Resultados
- 21. Discussion
- 23. CONCLUSIONS New techniques may improve better and reliable results There should be more invest on this kind of studies This study shed a light to develop better treatments. Radiationdoesnot cure, itsjust a waytopreventcancer spreads.
- 25. Simón Restrepo
- 27. Thanks