Appendix: Crash course in R and BioConductor

Training Manual Appendix 
Crash Course:
R and BioConductor
Jeff Skinner, M.S.
Sudhir Varma, Ph.D.
Bioinformatics and Computational Biosciences Branch (BCBB)
NIH/NIAID/OD/OSMO/OCICB
http://bioinformatics.niaid.nih.gov
ScienceApps@niaid.nih.gov

Crash Course: R and BioConductor 
2
Appendix
Solutions to Sample Problems for Students
#1. {Fisher’s iris data} Sir Ronald A. Fisher famously used this set of iris flower data
as an example to test his new linear discriminant statistical model. Now, the iris
data set is used as a historical example for new statistical classification models.
A) Search the help menu for the keyword “linear discriminant”, then report
the names of the functions and packages you find.
Ans. > help.search(“linear discriminant”) returns results for the
functions lda() and predict.lda() from the MASS package library.
B) Search the help menus or a search engine for additional classification
models that could be tested with the iris data.
Ans. Any results are OK, but two examples are the knn() function from the
class package library and the randomForest() function from the
randomForest package library.
C) The measurements from the iris data set were made in centimeters, but
suppose a researcher wanted to compare the performance of their classifier
for measurements in both cm and inches. Remember 1 cm = 0.3937 inch
and create a new iris data set with measurements in inches.
Ans. One possible answer is shown below:
> irisINCHES <- data.frame(0.3937*iris[,1:4],iris[,5])
> iris[1:4,]
Sepal.Length Sepal.Width Petal.Length Petal.Width Species
1 5.1 3.5 1.4 0.2 setosa
2 4.9 3.0 1.4 0.2 setosa
3 4.7 3.2 1.3 0.2 setosa
4 4.6 3.1 1.5 0.2 setosa
> irisINCHES[1:4,]
Sepal.Length Sepal.Width Petal.Length Petal.Width iris...5.
1 2.00787 1.37795 0.55118 0.07874 setosa
2 1.92913 1.18110 0.55118 0.07874 setosa
3 1.85039 1.25984 0.51181 0.07874 setosa
4 1.81102 1.22047 0.59055 0.07874 setosa

3
D) Use indexing to verify that the 77th
plant (i.e. row 77) has petal length of
approximately 1.89 inches.
Ans. Two possible answers are shown below:
> iris[77,"Petal.Length"]*0.3937
[1] 1.88976
> irisINCHES[77,3]
[1] 1.88976
#2. {AFP data} Suppose alpha-fetoprotein (AFP) is a potential biomarker for liver
cancer and other cancer types. A researcher might be interested in AFP levels
before and after taking a new drug in one of four concentrations.
A) The example in section 2.7.2 of the manual provided a list of 20 AFP
levels before drug treatment. Use your own methods to enter a new
column of 20 AFP levels after drug treatment, then enter another column
with the difference between the pre- and post-treatment AFP levels
Ans. One possible answer is shown below:
# manually enter Alpha-fetoprotein (AFP) levels for 20 patients
> AFP.after <- AFP.before - 1.2 + 0.2*rnorm(20)
> AFP.diff <- AFP.after - AFP.before
> afp.data <- data.frame(subject,gender,height,weight,BMI,
drug,AFP.before,AFP.after,AFP.diff)
> afp.data
B) Verify the storage mode of the data set afp.data. Verify the storage
mode of the variable drug. Verify the storage mode of the variable
gender. Convert the storage mode of drug to factor.
Ans. One possible answer shown below
> class(afp.data)
[1] "data.frame"
> class(afp.data$drug)
[1] "numeric"
> class(afp.data$gender)
[1] “factor”
> afp.data$drug <- as.factor(afp.data$drug)

4
C) Create a subset of the AFP data that only includes male patients with
BMI > 25.5 or weight > 180 lbs. How many men are included in the
data subset?
Ans. Six male patients are included in the subset. One example is shown:
> afp.subset <- afp.data[afp.data$gender=="male",]
> indx <- afp.subset$BMI > 25.5 | afp.subset$weight > 180
> afp.subset <- afp.subset[indx,]
> afp.subset
subject gender height weight BMI drug ...
2 2 male 69.15696 202.9318 29.82865 5 ...
3 3 male 69.35599 211.0632 30.84607 10 ...
5 5 male 71.44586 241.4526 33.25317 20 ...
7 7 male 68.21618 297.4155 44.93081 5 ...
8 8 male 69.77130 289.2935 41.77731 10 ...
10 10 male 66.95951 178.6660 28.01385 20 ...
D) Sort the entire data subset created in part C) by the BMI variable in an
descending order. What is the row ordering of the sorted data subset?
Save the data subset as a comma separated value (.csv) text file.
Ans. The row order is: 7, 8, 5, 3, 2, 10. A possible solution is below:
> afp.subset <- afp.subset[order(afp.subset$BMI,
decreasing=TRUE),]
> afp.subset
subject gender height weight BMI drug ...
7 7 male 68.21618 297.4155 44.93081 5 ...
8 8 male 69.77130 289.2935 41.77731 10 ...
5 5 male 71.44586 241.4526 33.25317 20 ...
3 3 male 69.35599 211.0632 30.84607 10 ...
2 2 male 69.15696 202.9318 29.82865 5 ...
10 10 male 66.95951 178.6660 28.01385 20 ...
> write.csv(afp.subset,file="~/subset.csv")
#3. {AE data} Doctors, epidemiologists and other researchers look at adverse events
to explore the symptoms and medical conditions affecting patients. A researcher
might choose to look for associations between adverse events and diet.
A) One of the adverse events in the data table is “Malaise”. Recode the AE
data table, such that all entries for “Malaise” read “Discomfort” instead.
Ans. Hint: you need to convert the adverse event variable to a character variable
> AE$Adverse.Event <- as.character(AE$Adverse.Event)
> indx <- AE$Adverse.Event == "Malaise"
> AE$Adverse.Event <- replace(AE$Adverse.Event,indx,"Discomfort")
> AE$Adverse.Event <- as.factor(AE$Adverse.Event)

5
B) Look at the results of your recoded adverse events. How many different
types of adverse events are there? Look through their names. Do you see
any potential problems? Fix any problems that you might find.
Ans. Initially, there are 18 different types of adverse events. There appears to
be a typo; “Mylagia” should be “Myalgia”. After correction, there are 17
different types of adverse events.
> length(levels(AE$Adverse.Event))
[1] 18
> AE$Adverse.Event <- as.character(AE$Adverse.Event)
> indx <- AE$Adverse.Event == "Mylagia"
> AE$Adverse.Event <- replace(AE$Adverse.Event,indx,"Myalgia")
> AE$Adverse.Event <- as.factor(AE$Adverse.Event)
> length(levels(AE$Adverse.Event))
[1] 17
C) Create an adverse event table to examine relationship between different
adverse event symptoms and their severities. Make sure the “Discomfort”
AE shows up in the table, instead of “Malaise”.
Ans. One possible solution is shown:
> attach(AE)
> AEtable <- table(Adverse.Event,Severity)
> AEtable
Severity
Adverse.Event Mild Moderate Severe
Anemia 2 3 1
Arthralgia 2 0 0
Dimpling 1 0 0
Discomfort 1 1 3
Ecchymosis 0 2 1
Elavated CH50 0 0 1
Erythema 0 3 1
Headache 1 5 0
Induration 1 3 0
Leukopenia 1 1 2
Myalgia 2 0 1
Nausea 4 0 1
Nodule 0 1 0
Pain 2 5 0
Papule 0 3 0
Swelling 1 2 1
Tenderness 2 2 1

6
D) Search the help menus for the functions rowSums and colSums. Use these
functions to count up the number of patients with each adverse event and
the number of patients with mild, moderate and severe symptoms.
Ans. An example is shown below
> AEsymptoms <- rowSums(AEtable)
> AEsymptoms
Anemia Arthralgia Dimpling Discomfort ...
6 2 1 5 ...
> AEseverity <- colSums(AEtable)
> AEseverity
Mild Moderate Severe
20 31 13
E) Define a new variable AEmatrix by converting the AE table into a matrix.
Define two new matrix variables: LL = matrix(1,1,17) and RR = c(1,1,1).
Compute the products of LL by AEmatrix; AEmatrix by RR; and LL by
AEmatrix by RR. Do you notice anything?
Ans. The matrix product LL by AEmatrix is equal to the colSums(), AEmatrix
by RR is equal to the rowSums() and LL by AEmatrix by RR is equal to
the sample size n = 64. An example is shown below:
> LL = matrix(1,1,17)
> RR = c(1,1,1)
> LL %*% AEmatrix
Severity
Mild Moderate Severe
[1,] 20 31 13
> AEmatrix %*% RR
Adverse.Event [,1]
Anemia 6
Arthralgia 2
Dimpling 1
Discomfort 5
Ecchymosis 3
Elavated CH50 1
Erythema 4
Headache 6
Induration 4
Leukopenia 4
Myalgia 3
Nausea 5
Nodule 1
Pain 7
Papule 3
Swelling 4
Tenderness 5
> LL %*% AEmatrix %*% RR
[,1]
[1,] 64

7
#4. {Fisher’s iris data} Sir Ronald A. Fisher famously used this set of iris flower data
as an example to test his new linear discriminant statistical model. Now, the iris
data set is used as a historical example for new statistical classification models.
A) Make a boxplot of all four measurements from Fisher’s iris data
Ans. An example is shown below:
> boxplot(iris[,1:4],main="Fisher's Iris Data",ylab="cm",
xlab="measurement",col="wheat")

8
B) Create a multi-panel figure with histograms of all four measurments. Do
you notice anything that could not be seen from the boxplot?
> par(mfrow=c(2,2))
> hist(iris[,1],main="Fisher's Iris Data -- Sepal Length",
ylab="count",xlab="Sepal Length (cm)",col="red")
> hist(iris[,2],main="Fisher's Iris Data -- Sepal Width",
ylab="count",xlab="Sepal Width (cm)",col="yellow")
> hist(iris[,3],main="Fisher's Iris Data -- Petal Length",
ylab="count",xlab="Petal Length (cm)",col="green")
> hist(iris[,4],main="Fisher's Iris Data -- Petal Width",
ylab="count",xlab="Petal Width (cm)",col="blue")
The boxplots didn’t show the bimodal distribution of petal length and
petal width, probably caused by differences among species.

9
C) Create a multi-panel figure with boxplots of all four measurements,
paneled by the three different species. Do you notice any differences
among species?
> par(mfrow=c(1,3))
> boxplot(iris[iris$Species=="setosa",1:4],
main="Fisher's Iris Data -- Setosa",ylab="cm",
xlab="measurement",col="wheat")
> boxplot(iris[iris$Species=="versicolor",1:4],
main="Fisher's Iris Data -- Versicolor",ylab="cm",
xlab="measurement",col="olivedrab")
> boxplot(iris[iris$Species=="virginica",1:4],
main="Fisher's Iris Data -- Virginica",ylab="cm",
xlab="measurement",col="grey")
Yes. There are big differences among the three species.

10
#5. {AFP data} Suppose alpha-fetoprotein (AFP) is a potential biomarker for liver
cancer and other cancer types. A researcher might be interested in AFP levels
before and after taking a new drug in one of four concentrations.
A) In section 3.2.1, the barplot() and arrows() commands were used to
create a barchart of mean(BMI) by gender with error bars. Install the
sciplot package library and use the bargraph.CI() command to
replicate that graph.
> library(sciplot)
> bargraph.CI(as.factor(afp.data$gender),afp.data$BMI,
col=c("pink","sky blue"),
main="Mean BMI by Gender",ylim=c(0,50),ylab="BMI")
> legend(x="topleft",legend=c("Female","Male"),
fill=c("pink","sky blue"))

11
B) Use the bargraph.CI() command to create a bar chart that compares AFP
difference over all five drug concentrations.
> bargraph.CI(as.factor(afp.data$drug),afp.data$AFP.diff,
col=rainbow(5),main="Mean AFP Difference by Drug",
ylim=c(0,-2),ylab="AFP difference",
xlab="Drug Concentration")
> legend(x="topleft",legend=seq(0,20,by=5),fill=rainbow(5),
title="Drug Concentration")

12
C) Create an interleaved bar chart that plots mean AFP difference by both
drug concentration and gender
> bargraph.CI(as.factor(afp.data$drug),afp.data$AFP.diff,
group=as.factor(afp.data$gender),
col=c("pink","sky blue"),
main="Mean AFP Difference by Drug and Gender",
ylim=c(0,-2),ylab="AFP difference",
xlab="Drug Concentration")
> legend(x="topleft",legend=c("Female","Male"),
fill=c("pink","sky blue"))
#6. {AE data} Doctors, epidemiologists and other researchers look at adverse events
to explore the symptoms and medical conditions affecting patients. A researcher
might choose to look for associations between adverse events and diet.
A) Create a histogram of Percent Body Fat (or your choice of continuous
response variable), then overlay a normal curve.

13
> norm.curve <- qnorm(seq(0,1,length=10000),
mean(AE$Percent.Body.Fat),
sd(AE$Percent.Body.Fat))
> hist(AE$Percent.Body.Fat,col="wheat",freq=FALSE,
xlab=”Percent Body Fat”)
> lines(density(norm.curve))
B) Install the lattice package and use the barchart() command to graph the
AEtable data table created for question #3. C) in the previous chapter.
What kind of plot is this? Add the appropriate figure legend.
Ans. The plot is a stacked bar chart, with stacked boxes representing the mild,
moderate and severe symptoms. An example is shown below:

14
> barchart(AEtable,main="Bar Chart of Adverse Event by Severity",
col=c("red","yellow","blue"))
> legend(x="topright",legend=levels(AE$Severity),
fill=c("red","yellow","blue"))
#7. {Nonparametric statistics} Search the help menus to find the command(s) for a
non-parametric statistical test analogous to the Student’s t-test (e.g. Mann-
Whitney U-test, Wilcoxon rank sum test, ...). Repeat at least one of the Student’s
t-test examples from section 4.1 with this non-parametric test.
> # Define a vector of % body fat data for men from AE data
> bfat.m <- AE[AE$Gender == "Male",6]
> # Define a vector of % body fat data for women from AE data
> bfat.f <- AE[AE$Gender == "Female",6]
> # Compute a two-sided, WIlcoxon Rank Sum test with AE data
> wilcox.test(bfat.m,bfat.f,alternative="two.sided")
Wilcoxon rank sum test with continuity correction
data: bfat.m and bfat.f
W = 553, p-value = 0.5811
alternative hypothesis: true location shift is not equal to 0
Warning message:
In wilcox.test.default(bfat.m, bfat.f, alt. = "two.sided") :
cannot compute exact p-value with ties

15
#8. {Linear models} Add a second predictor variable to the formula parameter of the
lm() procedure from the regression or ANOVA example in section 4.2 to create a
more complicated linear model. Use the AFP data.
Ans. An example of multiple regression is shown below:
> # Define afp.data data frame with stringsAsFactors FALSE
> afp.data <- data.frame(subject,gender,height,weight,BMI,drug,
AFP.before,AFP.after,AFP.diff,
stringsAsFactors=FALSE)
> # Call the lm() procedure to fit regression
> afp.reg <- lm(formula = AFP.diff ~ drug*BMI, data = afp.data)
> afp.reg
Call:
lm(formula = AFP.diff ~ drug * BMI, data = afp.data)
Coefficients:
(Intercept) drug BMI drug:BMI
-1.3568528 0.0123046 0.0049974 -0.0003010
> anova(afp.reg)
Analysis of Variance Table
Response: AFP.diff
Df Sum Sq Mean Sq F value Pr(>F)
drug 1 0.00863 0.00863 0.2017 0.6594
BMI 1 0.00384 0.00384 0.0897 0.7685
drug:BMI 1 0.00542 0.00542 0.1265 0.7267
Residuals 16 0.68512 0.04282
> summary(afp.reg)
Call:
lm(formula = AFP.diff ~ drug * BMI, data = afp.data)
Residuals:
Min 1Q Median 3Q Max
-0.26127 -0.12370 -0.01925 0.14384 0.40517
Coefficients:
Estimate Std. Error t value Pr(>|t|)
(Intercept) -1.3568528 0.3473496 -3.906 0.00126 **
drug 0.0123046 0.0268771 0.458 0.65325
BMI 0.0049974 0.0107781 0.464 0.64913
drug:BMI -0.0003010 0.0008463 -0.356 0.72670
---
Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
Residual standard error: 0.2069 on 16 degrees of freedom
Multiple R-Squared: 0.02545, Adjusted R-squared: -0.1573
F-statistic: 0.1393 on 3 and 16 DF, p-value: 0.935

16
#9. {Workflow scripting} Create a script to automate the creation graphing and linear
model analysis of the AFP data. Use your previous results from questions #2, #5
and #8, if necessary.
Ans. An example is shown:
############### Import AFP data ########################
# generate a list of subject IDs, numbered from 1 to 20
subject <- 1:20
# create 10 entries for male subjects
males <- rep("male",10)
# create 10 entries for female subjects
females <- rep("female",10)
# combine male and female entries into one column vector
gender <- c(males,females)
# bind subjectID and gender columns together
afp.data <- cbind(subject,gender)
# generate 10 male and 10 female random normal heights
height <- as.numeric(c(rnorm(10,70,2.5),rnorm(10,64,2.2)))
# generate 10 male and 10 female random uniform weights
weight <- as.numeric(c(runif(10,155,320),runif(10,95,210)))
# compute body mass index (BMI) for 10 men and 10 women
BMI <- as.numeric((weight*703)/(height**2))
# enter five treatment levels of a new drug (ng/mL)
drug <- rep(x = seq(from = 0, to = 20, by = 5), times = 4)
# manually enter Alpha-fetoprotein (AFP) levels for 20 patients
AFP.before <-
as.numeric(c(0.8,2.3,1.1,4.8,3.7,12.5,0.3,4.4,4.9,0.0,1.8,2.4,23.
6,8.9,0.7,3.3,3.1,0.5,2.7,4.5))
AFP.after <- AFP.before - 1.2 + 0.2*rnorm(20)
AFP.diff <- AFP.after - AFP.before

17
afp.data <-
data.frame(subject,gender,height,weight,BMI,drug,AFP.before,AFP.a
fter,AFP.diff)
afp.data
attach(afp.data)
############### Run LM regression ########################
afp.reg <- lm(formula = AFP.diff ~ drug*BMI, data = afp.data)
afp.reg
pdf("Regression.pdf")
par(mfrow = c(3,1))
plot(drug,AFP.diff,ylab="difference",main="regression plot")
abline(coef=afp.reg$coefficients[c(1,2)])
plot(BMI,AFP.diff,ylab="difference",main="regression plot")
abline(coef=afp.reg$coefficients[c(1,3)])
plot(afp.reg$fitted.values,afp.reg$residuals,xlab="fitted",ylab="
residual",main="residual plot")
dev.off()
browseURL("Regression.pdf")
############### Convert drug to factor ###################
afp.data$drug <- as.factor(afp.data$drug)
############### Run LM ANOVA #############################
afp.aov <- lm(formula = AFP.diff ~ drug, data = afp.data)
afp.aov
afp.anova <- anova(afp.aov)
afp.summary <- summary(afp.aov)
############## Plot graphs ##############################
library(sciplot)
pdf("ANOVA.pdf")
main = "One-way ANOVA"
ylab = "AFP differences"
xlab = "drug concentrations"
colors = rainbow(5)

18
means = afp.aov$fitted.values[1:5]
names(means) = levels(afp.data$drug)
mp <- barplot(height =
means,main=main,xlab=xlab,ylab=ylab,col=colors,ylim=c(0,-2))
X0 <- X1 <- mp
Y0 <- means - afp.summary$sigma
Y1 <- means + afp.summary$sigma
arrows(X0,Y0,X1,Y1,code=3,angle=90)
dev.off()
browseURL("ANOVA.pdf")
#10. {Function scripts} Create your own script to compute two new types of row
statistic (e.g. standard deviation and interquartile range) for a data frame or
matrix. Be creative, add graphics or a statistical test (e.g. linear regression).
# Define a function to compute row statistics with a for() loop
row.stats.loop <- function(x){
# Initialize vectors
row.sd <- row.IQR <- vector("numeric",length=nrow(x))
# Use a for() loop to compute means and medians for each
row
for(i in 1:nrow(x)){
row.sd[i] <- sd(x[i,])
row.IQR[i] <- IQR(x[i,])}
# Perform a linear regression
row.reg <- lm(formula = row.sd ~ row.IQR)
# Create a list of output
output <- list()
output[["row sd"]] <- row.sd
output[["row IQR"]] <- row.IQR
output[[“lm”]] <- row.reg
output[[“anova”]] <- anova(row.reg)
output[[“summary”]] <- summary(row.reg)
# Call the output list to report final results

19
output}
#11. Download the microarray dataset with the accession number “GDS10” from the
GEO website using the GEOquery package
Ans. The following loads the library, downloads the dataset and converts it to an
ExpressionSet object
library("GEOquery")
gds = getGEO("GDS10")
expset=GDS2eSet(gds, do.log2=TRUE)
A) Convert the data into three data frames, one for gene expression, one for
phenotypes and one for gene annotations
Ans. The following is an example script that will do this. Here we convert gds,
the output from getGEO() to an ExpressionSet object before converting
to the three data frames. We can do this directly from the getGEO() output
too (see the documentation for the GEOquery package on CRAN)
#Extract the expression matrix
X=exprs(expset)
#Extract the phenotypes
pheno.names=varLabels(expset)
> pheno.names
[1] "sample" "tissue" "strain"
"disease.state"
[5] "description"
phenotypes=data.frame(sample=expset$sample, tissue=expset$tissue,
strain=expset$strain, disease.state=expset$disease.state,
description=expset$description)
#Convert each row from factor to character type
for(i in 1:ncol(phenotypes))
phenotypes[,i]=as.character(phenotypes[,i])
#Extract the gene annotations
annot.columns= fvarLabels(expset)
> annot.columns
[1] "ID" "GB_ACC" "SPOT_ID"
annot.obj=featureData(expset)
annot=data.frame(id=annot.obj$ID, genbank.acc=annot.obj$GB_ACC,
spot.id=annot.obj$SPOT_ID)
B) Plot boxplots for each sample in one plot with different colors for each
sample. (Hint: use the stack() function and use a formula in the

20
boxplot() function. A vector of n colors can be obtained by using
rainbow(n))
Ans. The following is probably the easiest way to do this. You should look up
the help page for stack() to better understand how this works.
nsamp=ncol(X)
boxcol=rainbow(nsamp)
X.stack=stack(as.data.frame(X))
#Draw the boxplot
#Option las=3 makes the x axis labels vertical
boxplot(values~ind, data=X.stack, col=boxcol, las=3)
C) Compare the samples from the thymus and spleen for diabetic-resistant
mice and find the 10 most significant genes using the adjusted p-value.
Ans. This is a relatively lengthy script, but the explanation for each step can be
found here and in the manual.
#Find the samples that come from diabetic resistant mice
that
#originate from thymus
qt=which(phenotypes$disease.state=="diabetic-resistant" &
phenotypes$tissue=="thymus")
Xt=X[,qt]

21
#Find the samples that come from diabetic resistant mice
that
#originate from spleen
qs=which(phenotypes$disease.state=="diabetic-resistant" &
phenotypes$tissue=="spleen")
Xs=X[,qs]
#Compute the p-value and fold change for all genes
p.value=c()
fold.change=c()
for(i in 1:nrow(Xs))
{
#Find number of non-missing samples
n1=sum(!is.na(Xs[i,]))
n2=sum(!is.na(Xt[i,]))
if(n1 >= 2 & n2 >=2)
{
tt.res=t.test(Xs[i,], Xt[i,])
p.value[i]=tt.res$p.value
#The log fold change is calculated by the
#difference in means between the two classes
fold.change[i]=tt.res$estimate[2]-
tt.res$estimate[1]
}else
{
p.value[i]=NA
fold.change[i]=NA
}
}
#Compute adjusted p-values
adj.p.value=p.adjust(p.value)
#Find the smallest 10 p-values
qo=order(adj.p.value)
sig.genes=qo[1:10]
> adj.p.value[sig.genes]
[1] 1.859514e-12 7.615543e-12 1.852015e-11
[4] 3.337001e-11 4.210158e-11 5.769339e-11
[7] 7.557780e-11 9.369532e-11 1.125353e-10
[10] 1.331595e-10
D) Write the gene annotations, p-value, adjusted p-value and expressions in
all the samples for these 10 genes to an CSV file.
Ans. An example is shown below
d=data.frame(annot[sig.genes,], p.value=
p.value[sig.genes], adj.p.value=adj.p.value[sig.genes],
X[sig.genes,])
write.csv(d, file="report.csv", row.names=FALSE)

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