1. African horse sickness (AHS) is a vector-borne disease of equines caused by the African horse sickness virus. There are four clinical forms: pulmonary, cardiac, mixed, and horse sickness fever.
2. The pulmonary form is characterized by severe respiratory distress, coughing, and nasal discharge. Lesions include edema of the lungs and fluid in the chest cavity. The cardiac form shows edema of the head and neck. Mixed form shows signs of both pulmonary and cardiac forms. Horse sickness fever causes mild fever and is rarely diagnosed.
3. Laboratory confirmation is through virus isolation from blood or tissues, serological tests for antibodies, and PCR/RT-PCR tests. Differential diagnosis includes equine
Dr. Sachin Verma is a young, diligent and dynamic physician. He did his graduation from IGMC Shimla and MD in Internal Medicine from GSVM Medical College Kanpur. Then he did his Fellowship in Intensive Care Medicine (FICM) from Apollo Hospital Delhi. He has done fellowship in infectious diseases by Infectious Disease Society of America (IDSA). He has also done FCCS course and is certified Advance Cardiac Life support (ACLS) and Basic Life Support (BLS) provider by American Heart Association. He has also done a course in Cardiology by American College of Cardiology and a course in Diabetology by International Diabetes Centre. He specializes in the management of Infections, Multiorgan Dysfunctions and Critically ill patients and has many publications and presentations in various national conferences under his belt. He is currently working in NABH Approved Ivy super-specialty Hospital Mohali as Consultant Intensivists and Physician.
Avian Influenza (AI) is a viral influenza of birds including chickens, turkeys, guinea fowls, and other avian species.
There are three main types of Influenza (A,B & C).
Influenza Type-A
Multiple species
Influenza Type-B
Human
Influenza Type-C
Human & swine
Dr. Sachin Verma is a young, diligent and dynamic physician. He did his graduation from IGMC Shimla and MD in Internal Medicine from GSVM Medical College Kanpur. Then he did his Fellowship in Intensive Care Medicine (FICM) from Apollo Hospital Delhi. He has done fellowship in infectious diseases by Infectious Disease Society of America (IDSA). He has also done FCCS course and is certified Advance Cardiac Life support (ACLS) and Basic Life Support (BLS) provider by American Heart Association. He has also done a course in Cardiology by American College of Cardiology and a course in Diabetology by International Diabetes Centre. He specializes in the management of Infections, Multiorgan Dysfunctions and Critically ill patients and has many publications and presentations in various national conferences under his belt. He is currently working in NABH Approved Ivy super-specialty Hospital Mohali as Consultant Intensivists and Physician.
Avian Influenza (AI) is a viral influenza of birds including chickens, turkeys, guinea fowls, and other avian species.
There are three main types of Influenza (A,B & C).
Influenza Type-A
Multiple species
Influenza Type-B
Human
Influenza Type-C
Human & swine
Erysipelas is an infectious disease mostly of growing or adult swine.
The disease may be acute, subacute, or chronic.
Although acute septicemic swine erysipelas can result in a high mortality rate, the greatest economic loss probably occurs from the chronic, nonfatal forms of the disease.
It may be clinically inapparent, may cause
acute illness
involving many animals, Sudden and unexpected deaths
chronic disease characterized by
enlarged joints, lameness, and endocarditis.
Rhomboid skin (diamond-skin) lesions are an inconsistent feature only associated with acute cases
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Approximately 97.5% of the water on our planet is located in theoceans and is therefore classified as seawater. Freezing is one of the methods used for desalination.
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Position Statement on the Use of Dominance Theory in Behavior Modification of...Fanus van Straten
Dominance is defined as a relationship between
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The A to Z of Adobe Illustrator – design & illustrationFanus van Straten
The Adobe Illustrator toolbar is jam-packed with useful tools you may have used a million times or that may be entirely unfamiliar to you. Add to that the numerous panels and effects, and this program can create most any type of artwork. This A–Z list breaks down every tool in the Illustrator toolbar, with a link to a quick tip.
This document sets out general issues related to the
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Your Telkom Internet account provides you access to a vast
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Web, electronic mail, and File Transfer Protocol (FTP). Your
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CONTENTS
1 Introduction
2 Protecting Your Account
3 Account and Network Security
4 Electronic Mail
5 Protection of Network Performance
6 Personal Home Pages
7 Third Party Internet Relay Chat (IRC)
8 Illegal Activities
9 Fair usage policy
10 Breach of the Acceptable Use Policy
11 General
Search Engine Land’s Periodic Table Of SEO Success Factors is designed to depict the factors that will help your website succeed in gaining more visitors from organic search.
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Narrated Business Proposal for the Philadelphia Eaglescamrynascott12
Slide 1:
Welcome, and thank you for joining me today. We will explore a strategic proposal to enhance parking and traffic management at Lincoln Financial Field, aiming to improve the overall fan experience and operational efficiency. This comprehensive plan addresses existing challenges and leverages innovative solutions to create a smoother and more enjoyable experience for our fans.
Slide 2:
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African horse sickness diagnosis - Cape Town, South Africa
1. Livestock Health, Management and Production › High Impact Diseases › Vector-borne Diseases ›
African Horse Sickness ›
1 | P a g e
African Horse Sickness
Author: Dr Melvyn Quan
Adapted from: Coetzer, J.A.W and Guthrie, A.J. 2004. African horse sickness, in Infectious diseases of livestock,
edited by J.A.W. Coetzer & R.C. Tustin. Oxford University Press, Cape Town, 2: 1231-1264
Licensed under a Creative Commons Attribution license.
DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS
Clinical signs and pathology
Four clinical forms of AHS have been described – the “dunkop” or pulmonary, “dikkop” or cardiac, mixed
and horse sickness fever forms.
“Dunkop” or pulmonary form
This form of the disease occurs commonly when AHSV infects fully susceptible horses, notably
foals that have lost their passively acquired immunity. It is the form usually seen in dogs.
Following the incubation period, a fever may be the only sign for one or two days, with the rectal
temperature reaching 41°C or sometimes higher. The characteristic clinical signs in this form of
AHS are severe dyspnoea, paroxysms of coughing, and sometimes, discharge of large quantities
of frothy, serofibrinous fluid from the nostrils.
2. Livestock Health, Management and Production › High Impact Diseases › Vector-borne Diseases ›
African Horse Sickness ›
2 | P a g e
Discharge of large quantities of frothy, serofibrinous fluid from the nostrils
are seen in some horses with the pulmonary form of African horse sickness prior to death
In most cases the discharge from the nose only appears after death. The appetite of affected
animals remains good in spite of the high fever and respiratory distress. Sometimes the animal
may take a mouthful of hay without chewing it. The onset of dyspnoea is generally very sudden,
and death often occurs within a few hours of its appearance.
The prognosis for horses suffering from the “dunkop” form is extremely grave; less than five per
cent recover. In recovering horses the fever gradually subsides but the breathing remains
laboured for some days.
The most striking changes are severe oedema of the lungs and hydrothorax. Several litres of pale
yellow fluid which may coagulate on exposure to air are found in the thoracic cavity.
3. Livestock Health, Management and Production › High Impact Diseases › Vector-borne Diseases ›
African Horse Sickness ›
3 | P a g e
In the pulmonary form the trachea contains
large amounts of froth
Froth and serofibrinous fluid which may be
gelatinous in the trachea of a horse that died of
the pulmonary form of the disease
Severe oedema of the interlobular septa of the lungs
Oedema of the subcutaneous and intermuscular tissues is usually absent or mild. The
mediastinum, and often the loose connective tissue around major blood vessels in the thorax,
oesophagus, trachea and thymus, may also be oedematous.
Hydropericardium is rare in this form of the disease. Epi- and endocardial haemorrhages of
varying size (particularly severe in the left ventricle), sometimes accompanied by slight oedema
of the fat in the coronary grooves and of the atrioventricular valves, may be evident.
Marked diffuse congestion of the mucosa of the glandular part of the stomach is a consistent
finding. This may be accompanied by patchy congestion and petechiation of the serosa and,
sometimes, of the mucosa of the intestine. The liver may be slightly enlarged and congested, and
its lobulation slightly more distinct than normal. There is usually some degree of ascites.
4. Livestock Health, Management and Production › High Impact Diseases › Vector-borne Diseases ›
African Horse Sickness ›
4 | P a g e
“Dikkop” or cardiac form
This form of AHS is characterized by subcutaneous oedema of the head and neck, and
particularly the supraorbital fossae.
Facial swelling and oedema of the
supraorbital fossae
The oedema usually appears late in the course of the disease, but if it appears early, the
condition is more serious, more acute and with a higher rate of mortality. In severe cases, there
may be oedema of the eyelids, lips, cheeks, tongue, intermandibular space, and sometimes also
the neck, chest and shoulders, but usually not the lower parts of the legs (i.e. below the elbow or
stifle joints).
Oedema of the supraorbital
fossa and eye lids
Severe oedema of the eyelids in a horse
suffering from African horse sickness
5. Livestock Health, Management and Production › High Impact Diseases › Vector-borne Diseases ›
African Horse Sickness ›
5 | P a g e
As the oedema worsens, dyspnoea and cyanosis may supervene.
Fever peaks at a later stage in the course of the disease than in the “dunkop” form, and may
remain high for three to six days before declining. Some animals will only develop a very mild
fever. Some animals may lie down repeatedly or are restless when standing, and frequently paw
the ground with their front feet as a result of severe colic. Interference or difficulty in swallowing
as a result of paralysis of the oesophagus may be a complication, particularly in those cases
where there is severe oedema of the head.
The “dikkop” form of AHS is always more protracted and milder than the “dunkop” form, with a
mortality rate of about 50 per cent. Death usually occurs within four to eight days of the onset of
the febrile reaction. Unfavourable prognostic signs are petechiae in the mucosa of the
conjunctivae and mouth, on the ventral aspect of the tongue. These, if they occur, are evident
shortly before death.
The most characteristic pathological change is yellowish gelatinous oedema of the subcutaneous
and intermuscular connective tissues of the head and neck.
Severe oedema of the intermuscular
connective tissue
Yellowish gelatinous oedema of the subcutaneous
and intermuscular connective tissue
6. Livestock Health, Management and Production › High Impact Diseases › Vector-borne Diseases ›
African Horse Sickness ›
6 | P a g e
Yellowish gelatinous oedema of the
subcutaneous tissues
Severe oedema of the intermuscular
connective tissues
In severe cases this extends to the back, shoulders and chest. The oedema is particularly severe
around the ligamentum nuchae.
Lesions similar to, but more severe than those described for the “dunkop” form, are found in the
heart. Pale greyish blanched areas of varying size may very rarely be noticed in the myocardium.
Severe hydropericardium is almost invariably present.
Hydropericardium
The lungs are usually normal or slightly congested, and only slightly oedematous. The thoracic
cavity seldom contains an excess of fluid. Many lymph nodes are swollen and oedematous but
the spleen is occasionally only slightly enlarged. The liver is engorged and its lobulation distinct.
There is usually mild nephrosis.
7. Livestock Health, Management and Production › High Impact Diseases › Vector-borne Diseases ›
African Horse Sickness ›
7 | P a g e
Lesions in the gastrointestinal tract are similar to, but more severe than, those found in the
“dunkop” form. In cases which manifest severe paralysis of the oesophagus, the organ is
distended with a variable amount of compressed food. Moderate to severe oedema, congestion
and petechiation of the mucosa of the caecum, colon and rectum are regular findings.
Oedema, congestion and petechiation of the
intestines
Severe oedema and petechiation of the
mucosa of the large intestine
Severe diffuse congestion of the glandular
part of the stomach
Mixed form
Although this is the most common form of AHS it is very rarely diagnosed as such clinically; one
of the other preceding forms predominates and this is reflected in the diagnosis. It is only during
necropsy when lesions of both the “pulmonary” and “cardiac” forms are observed that the
diagnosis is made.
8. Livestock Health, Management and Production › High Impact Diseases › Vector-borne Diseases ›
African Horse Sickness ›
8 | P a g e
Horses affected by this form may show signs either of respiratory distress followed by
oedematous swellings or, initially, of the “dikkop” form before suddenly developing respiratory
distress from which they may die. The mortality rate in horses affected by this form of the disease
is approximately 70 per cent. Death usually follows three to six days after the onset of the febrile
reaction.
Horse sickness fever
This form usually occurs in horses immune to one or more serotypes of AHSV which become
infected with a heterologous serotype against which there is some cross protection. It also occurs
in species such as donkeys and zebras which are resistant to the development of clinical disease.
Horse sickness fever is a very mild form of the disease and is frequently not diagnosed clinically.
The most characteristic finding is a rise of the rectal temperature (39 to 40 °C) lasting one to six
days followed by a drop in temperature to normal, and recovery. Some horses may show partial
loss of appetite, congestion of the conjunctivae, slightly laboured breathing, and increased heart
rate, but these signs are transient.
9. Livestock Health, Management and Production › High Impact Diseases › Vector-borne Diseases ›
African Horse Sickness ›
9 | P a g e
Laboratory confirmation
The epidemiology, clinical signs and gross lesions of AHS are often sufficiently specific to allow a
provisional diagnosis of the disease to be made. To confirm the diagnosis, the virus should be isolated
from blood collected, preferably in heparin-containing tubes, during the febrile stage of the disease or
from specimens of the lungs, spleen and lymph nodes collected at necropsy and kept at 4 °C. In fatal
cases high levels of infectivity are found in the lungs, spleen and lymph nodes. Virus isolation may be
achieved by the use of a variety of cell cultures (e.g. BHK-21, VERO, monkey stable), by intracerebral
inoculation of suckling mice or by intravenous inoculation of embryonated hens' eggs. When heparin is
not available, blood collected in chelating agents such as Na-EDTA should be diluted five to ten fold to
prevent detachment of the monolayer of cell cultures.
Virus isolates are identified by group-specific tests such as complement fixation (CF), agar gel
immunodiffusion (AGID), direct and indirect immunofluorescence (IFA) or enzyme-linked immunosorbent
assay (ELISA). Serotyping of AHS virus isolates is performed using virus neutralization (VN) tests
employing type-specific antisera. Virus neutralization tests may be conducted in mice or various cell
cultures e.g. plaque neutralization. Antibody to AHSV can be detected by utilizing CF, AGID, IFA, VN and
ELISA tests.
In horses that have recovered from the disease, high CF antibody titres indicate infection with the virus
within the previous few months. Antibodies detectable by VN tests and ELISA persist for a number of
years.
A number of conventional reverse transcription polymerase chain reaction (RT-PCR) assays and real-
time reverse transcription PCR assays have been developed for the detection of AHSV and to
differentiate between the serotypes of AHSV. Genomic probes can also be developed and applied for in
situ hybridization in tissues. Immunohistochemical staining methods have also been used successfully to
determine the localization of AHS antigen within various tissues. The advantages of these new
approaches are that they have the potential to be rapid, sensitive and versatile, and they may supplement
or replace some of the older conventional methods. Furthermore, they can be applied to specimens from
clinical cases that do not contain live virus. Immunohistochemical staining can be applied to tissues that
have been preserved in 10% buffered formalin, which is an advantage if it is not possible for the samples
to reach the laboratory rapidly.
Differential diagnosis
It is not possible to differentiate the AHS fever form of the disease from the early febrile stages of other
forms of AHS, or from the early febrile stages of many other equine infectious diseases.
10. Livestock Health, Management and Production › High Impact Diseases › Vector-borne Diseases ›
African Horse Sickness ›
10 | P a g e
The clinical signs and lesions of AHS may be confused with those of equine encephalosis. As many of
the epidemiological features of the two diseases are similar, they may occur simultaneously in South
Africa and possibly also in other regions in southern Africa. Horses manifesting swelling of the eyelids,
supraorbital fossae or of the entire head as a result of equine encephalosis cannot be differentiated
clinically from the “dikkop” form of AHS. However, the mortality rate of AHS is much higher than is that of
equine encephalosis. Virus isolation and identification or serological tests on paired serum samples are
essential to confirm either diagnosis.
Although the lesions of AHS at necropsy are fairly characteristic, the disseminated small haemorrhages
and the oedema found in some parts of the body that are associated with the “dikkop” form of AHS may
be very similar to those found in cases of purpura haemorrhagica and equine viral arteritis. In horses
affected by these conditions, subcutaneous oedema often occurs ventral to the elbow and stifle joints, as
well as in other locations, while in those suffering from AHS it does not occur below these joints. In
purpura haemorrhagica, oedema and haemorrhages tend to be more numerous, widespread and severe
than in AHS.
The early stages of piroplasmosis, when Babesia/Theileria parasites may be difficult to demonstrate in
blood smears, are occasionally confused with AHS. AHS may also be complicated by piroplasmosis, and
in such cases ventral oedema may be severe.