content of this slide: 1. Advantage of Thin Layer Chromatography. 2. Limitation of Thin Layer Chromatography. 3. Application of Thin Layer Chromatography. 4. Argentation Thin Layer Chromatography.

1. Thin LayerThin Layer
ChromatographyChromatography
Presented byPresented by

3. Advantage &
Limitation of
T.L.C.

4. Advantage of T.L.C.Advantage of T.L.C.
• This is a very easy way to
separate the components.
• TLC is a sensitive method.
• It is feasible to visualize all
components of UV light.

5. • The non-volatile compounds are
separated by the TCL method.
• In comparison to other separation
techniques, very few types of
equipment are used. The
components are separated in a
very short time because the
components will elute rapidly.

6. • The only small sample size is
required in TLC, and it can be in
microlitre.
• The components there in the
complex mixture of samples are
able to easily separate and
recovered by scratching the
plate.

7. Limitatio
n of
T.L.C.

8. Limitation of T.L.C.
• Results obtained from TLC are
difficult to reproduce.
• Only soluble components of
the mixtures are possible.
• This is the only qualitative
analysis possible, not
quantitative.

9. Limitation of T.L.C.
• Usually, it is not automatic.
• TCL works in the open
system, therefore, due to
temperature and humidity
can affect the results.

10. Application
Of
T.L.C.

16. Argentation T.L.C.Argentation T.L.C.

17. A form of thin
layer
chromatography
in which a
stationary phase
consisting of
silica coated
with silver
nitrate is used to
separate cis- and
trans- fatty acid
derivatives.
Argentation T.L.C.

18. Here, Schematic
separation of methyl
ester derivatives of
unsaturated fatty acids
by Argentation TLC on
silica gel G impregnated
with 10 % (w/w) silver
nitrate.
Plate A: mobile phase
hexane-diethyl ether
(9:1, v/v).
Plate B: as A but
solvents in the ratio 2:3.