Thin layer chromatography (TLC) is a chromatography technique used to separate mixtures. It uses a stationary phase, typically silica gel on a glass or aluminum plate, and a mobile phase, usually a solvent or solvent mixture, to separate compounds in a mixture. High performance TLC (HPTLC) is an advanced form of TLC that provides better separation using optimized stationary phases with smaller particle sizes and tighter size distributions compared to normal TLC plates. The key steps in TLC and HPTLC involve preparing the chromatographic plate, applying the sample as a spot, developing the plate with a mobile phase, and detecting separated components using visualization techniques. TLC and HPTLC have various applications in analyzing organic compounds,
Chromatography is a physical separation technique that separates components between two phases, one stationary and one mobile. It has been used since 1900 to separate plant pigments. There are several chromatography techniques including thin layer chromatography (TLC), column chromatography, high performance liquid chromatography (HPLC), and gas chromatography (GC). Advanced techniques include affinity, size-exclusion, simulated moving-bed, and chiral chromatography. Chromatography has applications in Ayurveda for drug identification, standardization of herbs and formulations, assessing fermentation reactions, and developing fingerprints of single and polyherbal formulations.
Here are the key principles of the different chromatography techniques covered in the document:
- Paper chromatography relies on the differential migration rates of compounds through a stationary phase (filter paper) based on their varying interactions with the mobile phase solvent. Components separate based on differences in solubility and affinity for the mobile vs. stationary phases.
- Thin layer chromatography uses a thin stationary phase coating (e.g. silica gel) on a plate. Components separate based on differences in their partitioning behavior between the mobile liquid phase and stationary solid phase during capillary movement.
- Column chromatography uses a packed stationary phase within a column. Components separate based on differences in their distribution between the stationary phase adsorbent and percolating mobile phase
Column chromatography is a technique used to separate chemical compounds in a mixture based on how they interact differently with the stationary and mobile phases in the column. Compounds move through the column at different rates depending on their affinity for the stationary phase, allowing them to elute from the column in separate fractions over time. This technique can be used on both small and large scales to purify compounds for use in experiments by exploiting differences in their adsorption properties.
Thin Layer Chromatography - TLC- by Aloo Denish and Oloo Boniface.pdfDenish Aloo
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Thin Layer Chromatography (TLC)
By Aloo D. and Oloo B.
- Principle of TLC
-Components of TLC
-Procedure of TLC
-Interpretation of TLC Results
-Advances in TLC
-TLC Techniques - Coupling TLC
-Thin-layer radiochromatography (TLRC)
-Application of TLC
Thin layer chromatography (TLC) is a technique used to separate mixtures based on differences in how compounds partition between a stationary phase and a mobile phase. TLC uses a glass, plastic, or aluminum plate coated with a thin layer of adsorbent material (typically silica gel) as the stationary phase. A sample mixture is applied near one edge of the plate, and the plate is placed in a developing chamber with a small amount of mobile phase solvent. As the solvent rises through the plate by capillary action, it carries different analytes with it at different rates based on their interactions with the two phases, separating the mixture into individual components visible as spots on the plate. TLC is a simple, inexpensive technique
Thin layer chromatography (TLC) is a chromatography technique used to separate mixtures. It uses a stationary phase, typically silica gel on a glass or aluminum plate, and a mobile phase, usually a solvent or solvent mixture, to separate compounds in a mixture. High performance TLC (HPTLC) is an advanced form of TLC that provides better separation using optimized stationary phases with smaller particle sizes and tighter size distributions compared to normal TLC plates. The key steps in TLC and HPTLC involve preparing the chromatographic plate, applying the sample as a spot, developing the plate with a mobile phase, and detecting separated components using visualization techniques. TLC and HPTLC have various applications in analyzing organic compounds,
Chromatography is a physical separation technique that separates components between two phases, one stationary and one mobile. It has been used since 1900 to separate plant pigments. There are several chromatography techniques including thin layer chromatography (TLC), column chromatography, high performance liquid chromatography (HPLC), and gas chromatography (GC). Advanced techniques include affinity, size-exclusion, simulated moving-bed, and chiral chromatography. Chromatography has applications in Ayurveda for drug identification, standardization of herbs and formulations, assessing fermentation reactions, and developing fingerprints of single and polyherbal formulations.
Here are the key principles of the different chromatography techniques covered in the document:
- Paper chromatography relies on the differential migration rates of compounds through a stationary phase (filter paper) based on their varying interactions with the mobile phase solvent. Components separate based on differences in solubility and affinity for the mobile vs. stationary phases.
- Thin layer chromatography uses a thin stationary phase coating (e.g. silica gel) on a plate. Components separate based on differences in their partitioning behavior between the mobile liquid phase and stationary solid phase during capillary movement.
- Column chromatography uses a packed stationary phase within a column. Components separate based on differences in their distribution between the stationary phase adsorbent and percolating mobile phase
Column chromatography is a technique used to separate chemical compounds in a mixture based on how they interact differently with the stationary and mobile phases in the column. Compounds move through the column at different rates depending on their affinity for the stationary phase, allowing them to elute from the column in separate fractions over time. This technique can be used on both small and large scales to purify compounds for use in experiments by exploiting differences in their adsorption properties.
Thin Layer Chromatography - TLC- by Aloo Denish and Oloo Boniface.pdfDenish Aloo
Â
Thin Layer Chromatography (TLC)
By Aloo D. and Oloo B.
- Principle of TLC
-Components of TLC
-Procedure of TLC
-Interpretation of TLC Results
-Advances in TLC
-TLC Techniques - Coupling TLC
-Thin-layer radiochromatography (TLRC)
-Application of TLC
Thin layer chromatography (TLC) is a technique used to separate mixtures based on differences in how compounds partition between a stationary phase and a mobile phase. TLC uses a glass, plastic, or aluminum plate coated with a thin layer of adsorbent material (typically silica gel) as the stationary phase. A sample mixture is applied near one edge of the plate, and the plate is placed in a developing chamber with a small amount of mobile phase solvent. As the solvent rises through the plate by capillary action, it carries different analytes with it at different rates based on their interactions with the two phases, separating the mixture into individual components visible as spots on the plate. TLC is a simple, inexpensive technique
The document provides an overview of column chromatography, including its history, key components, principles, procedures, applications, and advantages/limitations. Column chromatography is a separation technique that uses a stationary phase (often silica gel or alumina) packed into a column and a liquid or gas mobile phase to separate mixtures based on how strongly components interact with the stationary phase. It was developed in 1901 and is widely used today to purify compounds and remove impurities from mixtures.
Thin layer chromatography is a technique used to separate non-volatile mixtures. It works by applying samples as spots on a thin layer of adsorbent material like silica gel coated on a plate or sheet. A solvent or solvent mixture is drawn up the plate by capillary action, causing different analytes to ascend at different rates, achieving separation. Factors like the mobile phase, stationary phase, time of contact affect the retardation factor (Rf) value which can be used to identify compounds. TLC has applications in monitoring reactions, identifying mixtures, and determining purity.
Thin layer chromatography is a technique where a liquid sample is separated into components by migrating up a thin layer of adsorbent material like silica gel. The sample components separate due to differences in how strongly they interact with the stationary and mobile phases. A TLC system includes plates coated with adsorbent material, a chamber to develop the plates with a mobile phase like solvents, and methods to visualize results like UV light or iodine staining. TLC is used to analyze mixtures like pharmaceuticals and cosmetics due to being a simple, fast, and inexpensive separation method.
Column chromatography is a separation technique that uses a column packed with a stationary phase to separate mixtures based on how compounds partition between the stationary and mobile phases. Martin and Synge introduced partition column chromatography in 1941 using differences in how compounds partition between two liquid phases. Column chromatography can use a solid stationary phase for adsorption chromatography or a liquid stationary phase for partition chromatography. The technique works by selectively retaining compounds based on their interaction with and attraction to the stationary phase.
This is about on TLC. and I hope it will helpful for you.
In this describe about their introduction, principle, application, procedure, methodology, RF value, and their advantage, disadvantage
Thank you😊
introduction, history, principle, experimental techniques, evaluation on chromatogram, adv. & dis-adv., common problems, comparision, applications and analysis of drugs through TLC(2000-2017)
Types of Chromatography(Stationary Phase).pptxPriyaDixit46
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Types of chromatography are classified based on their stationary phase. Thin layer chromatography uses a thin layer of adsorbent like silica gel or aluminum sheet as the stationary phase. Paper chromatography uses filter paper as the stationary phase. Column chromatography uses an adsorbent material like silica gel packed in a glass column as the stationary phase. The document further describes the basic principles, procedures, and applications of these chromatography techniques.
Thin layer chromatography (TLC) is a chromatography technique used to separate mixtures by distributing the components between a stationary phase, such as silica gel coated on a plate, and a mobile phase, such as a solvent mixture, which moves up the plate by capillary action. TLC involves spotting a sample mixture onto the plate, developing it in a solvent system, and visualizing the separated components, which travel at different rates depending on how they partition between the stationary and mobile phases. TLC is a simple, fast, and inexpensive analytical technique used for qualitative and quantitative analysis of organic compounds and testing compound purity.
Thin layer chromatography (TLC) is a method used to separate mixtures by distributing components between two phases - a stationary phase coated on a plate and a mobile phase that moves over the plate. TLC involves applying samples to a plate coated with adsorbent material like silica gel. A solvent is drawn up the plate by capillary action, separating the mixture into individual components visualized as spots. TLC is used to identify unknown compounds, determine purity, and monitor reactions by calculating retention factor values.
Chromatograhpy, and column chromatography.Mohamed Samy
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Chromatography is a method of separating mixtures into individual components based on differences in how they interact with and move through stationary and mobile phases. There are various types of chromatography classified by the separation principle used and phases involved, including adsorption, partition, ion exchange, and size exclusion. Column chromatography is commonly used, involving a solid stationary phase packed into a column with a liquid mobile phase passed through to separate components. Factors like choice of stationary phase, mobile phase, development technique affect the separation achieved.
Chromatography is a technique used to separate and identify the components of a mixture. It works by allowing the molecules present in the mixture to distribute themselves between a stationary and a mobile medium. Molecules that spend most of their time in the mobile phase are carried along faster. Thin layer chromatography (TLC) is a type of chromatography where the stationary phase is a thin layer of adsorbent coated on a plate and the mobile phase is a liquid that travels up the plate, separating the components based on how strongly they adsorb to the stationary phase. Paper chromatography uses a paper strip as the stationary phase, with an organic solvent as the mobile phase.
Thin layer chromatography (TLC) is a technique used to separate components of a mixture based on their affinity to a stationary phase and a mobile phase. In TLC, a mixture is applied to a thin layer of adsorbent material like silica gel coated on a plate. A mobile phase like a solvent is drawn up the plate via capillary action, causing different components to ascend at different rates based on their interactions with the phases, achieving separation. Once separated, the individual components can be visualized as spots on the plate and identified. TLC is useful for analyzing mixtures, determining purity, and identifying unknown compounds.
Thin layer chromatography by khaleel S.GKhaleel Basha
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Thin layer chromatography (TLC) is introduced as a method for separating mixtures into individual components using an adsorbent solid spread over a glass plate and a liquid mobile phase. The document discusses the principles, equipment, experimental procedures, factors affecting resolution, and applications of TLC. Key factors that influence separation by TLC include the choice of stationary and mobile phases, preparation of uniform adsorbent layers, and detection of separated components. TLC is widely used in pharmaceutical analysis, organic synthesis, and biochemical research due to its simplicity, speed, sensitivity, and ability to separate small quantities of substances.
Thin layer chromatography is a technique used to separate mixtures based on differences in how compounds partition between a stationary phase and mobile phase. It involves applying sample spots to a thin silica coated plate then developing in a solvent mobile phase, causing compounds to migrate different distances. The ratio of a compound's migration distance to the solvent front distance is its Rf value, which can identify compounds by comparison to standards. Thin layer chromatography has applications in pharmaceutical analysis, food and cosmetic testing, and biochemical analysis to identify compounds, assess purity, and detect impurities.
Thin layer chromatography- Advances of Paper Chromatographysadia muhammad din
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Thin layer chromatography (TLC) is a technique used to separate mixtures by distributing the components between a stationary phase, such as silica gel coated onto a plate, and a mobile phase, such as a solvent that moves across the plate. TLC involves spotting a sample onto the plate, developing it by allowing the mobile phase to travel up the plate, and visualizing the separated components, which travel at different rates depending on how they partition between the phases. TLC is a simple, fast, and inexpensive method used to analyze organic compounds and test purity across various fields like pharmaceuticals, clinical chemistry, and food analysis.
This document provides information on three chromatography techniques: thin layer chromatography (TLC), paper chromatography, and column chromatography. It describes the basic principles, components, procedures, and applications of each technique. TLC involves separating compounds on a thin stationary phase using a mobile phase. Paper chromatography uses paper as the stationary phase. Column chromatography uses a column packed with an adsorbent stationary phase to separate mixtures based on compound affinity. Each technique can be used to analyze and purify mixtures of compounds.
This document provides an overview of chromatography techniques. It begins with a brief history of chromatography from 1903 to 1965. It then discusses the importance of chromatography and its applications in various fields. The document defines chromatography and describes various techniques including thin layer chromatography, gas chromatography, high performance liquid chromatography, and ion exchange chromatography. It explains the theoretical bases and factors affecting chromatographic separation. Finally, it discusses terminology, applications, and detection methods in chromatography.
This document provides information on column chromatography and gas chromatography techniques used in research. Column chromatography separates compounds based on differential rates of movement through an adsorbent column. It involves a stationary phase, mobile phase, and sample mixture. Gas chromatography also separates compounds based on partitioning between a gas mobile phase and stationary phase coating. It outlines the procedure, applications, advantages, and limitations of both techniques.
Chromatography separates components in a mixture using a stationary and mobile phase. High performance liquid chromatography (HPLC) is a type of chromatography that uses high pressure to force a liquid mobile phase through a column packed with solid particles. The document discusses various aspects of HPLC including separation modes, selecting stationary and mobile phases, HPLC system components, and applications.
Chromatography is a technique used to separate mixtures by exploiting differences in how components interact with stationary and mobile phases. The document discusses several types of chromatography including column chromatography, paper chromatography, thin layer chromatography, gas chromatography, and high performance liquid chromatography. It provides details on the principles, instrumentation, and procedures for each type. The key points are that chromatography separates mixtures based on how components partition between stationary and mobile phases, resulting in separation over time. A variety of stationary and mobile phases can be used depending on the application and components.
The document provides an overview of column chromatography, including its history, key components, principles, procedures, applications, and advantages/limitations. Column chromatography is a separation technique that uses a stationary phase (often silica gel or alumina) packed into a column and a liquid or gas mobile phase to separate mixtures based on how strongly components interact with the stationary phase. It was developed in 1901 and is widely used today to purify compounds and remove impurities from mixtures.
Thin layer chromatography is a technique used to separate non-volatile mixtures. It works by applying samples as spots on a thin layer of adsorbent material like silica gel coated on a plate or sheet. A solvent or solvent mixture is drawn up the plate by capillary action, causing different analytes to ascend at different rates, achieving separation. Factors like the mobile phase, stationary phase, time of contact affect the retardation factor (Rf) value which can be used to identify compounds. TLC has applications in monitoring reactions, identifying mixtures, and determining purity.
Thin layer chromatography is a technique where a liquid sample is separated into components by migrating up a thin layer of adsorbent material like silica gel. The sample components separate due to differences in how strongly they interact with the stationary and mobile phases. A TLC system includes plates coated with adsorbent material, a chamber to develop the plates with a mobile phase like solvents, and methods to visualize results like UV light or iodine staining. TLC is used to analyze mixtures like pharmaceuticals and cosmetics due to being a simple, fast, and inexpensive separation method.
Column chromatography is a separation technique that uses a column packed with a stationary phase to separate mixtures based on how compounds partition between the stationary and mobile phases. Martin and Synge introduced partition column chromatography in 1941 using differences in how compounds partition between two liquid phases. Column chromatography can use a solid stationary phase for adsorption chromatography or a liquid stationary phase for partition chromatography. The technique works by selectively retaining compounds based on their interaction with and attraction to the stationary phase.
This is about on TLC. and I hope it will helpful for you.
In this describe about their introduction, principle, application, procedure, methodology, RF value, and their advantage, disadvantage
Thank you😊
introduction, history, principle, experimental techniques, evaluation on chromatogram, adv. & dis-adv., common problems, comparision, applications and analysis of drugs through TLC(2000-2017)
Types of Chromatography(Stationary Phase).pptxPriyaDixit46
Â
Types of chromatography are classified based on their stationary phase. Thin layer chromatography uses a thin layer of adsorbent like silica gel or aluminum sheet as the stationary phase. Paper chromatography uses filter paper as the stationary phase. Column chromatography uses an adsorbent material like silica gel packed in a glass column as the stationary phase. The document further describes the basic principles, procedures, and applications of these chromatography techniques.
Thin layer chromatography (TLC) is a chromatography technique used to separate mixtures by distributing the components between a stationary phase, such as silica gel coated on a plate, and a mobile phase, such as a solvent mixture, which moves up the plate by capillary action. TLC involves spotting a sample mixture onto the plate, developing it in a solvent system, and visualizing the separated components, which travel at different rates depending on how they partition between the stationary and mobile phases. TLC is a simple, fast, and inexpensive analytical technique used for qualitative and quantitative analysis of organic compounds and testing compound purity.
Thin layer chromatography (TLC) is a method used to separate mixtures by distributing components between two phases - a stationary phase coated on a plate and a mobile phase that moves over the plate. TLC involves applying samples to a plate coated with adsorbent material like silica gel. A solvent is drawn up the plate by capillary action, separating the mixture into individual components visualized as spots. TLC is used to identify unknown compounds, determine purity, and monitor reactions by calculating retention factor values.
Chromatograhpy, and column chromatography.Mohamed Samy
Â
Chromatography is a method of separating mixtures into individual components based on differences in how they interact with and move through stationary and mobile phases. There are various types of chromatography classified by the separation principle used and phases involved, including adsorption, partition, ion exchange, and size exclusion. Column chromatography is commonly used, involving a solid stationary phase packed into a column with a liquid mobile phase passed through to separate components. Factors like choice of stationary phase, mobile phase, development technique affect the separation achieved.
Chromatography is a technique used to separate and identify the components of a mixture. It works by allowing the molecules present in the mixture to distribute themselves between a stationary and a mobile medium. Molecules that spend most of their time in the mobile phase are carried along faster. Thin layer chromatography (TLC) is a type of chromatography where the stationary phase is a thin layer of adsorbent coated on a plate and the mobile phase is a liquid that travels up the plate, separating the components based on how strongly they adsorb to the stationary phase. Paper chromatography uses a paper strip as the stationary phase, with an organic solvent as the mobile phase.
Thin layer chromatography (TLC) is a technique used to separate components of a mixture based on their affinity to a stationary phase and a mobile phase. In TLC, a mixture is applied to a thin layer of adsorbent material like silica gel coated on a plate. A mobile phase like a solvent is drawn up the plate via capillary action, causing different components to ascend at different rates based on their interactions with the phases, achieving separation. Once separated, the individual components can be visualized as spots on the plate and identified. TLC is useful for analyzing mixtures, determining purity, and identifying unknown compounds.
Thin layer chromatography by khaleel S.GKhaleel Basha
Â
Thin layer chromatography (TLC) is introduced as a method for separating mixtures into individual components using an adsorbent solid spread over a glass plate and a liquid mobile phase. The document discusses the principles, equipment, experimental procedures, factors affecting resolution, and applications of TLC. Key factors that influence separation by TLC include the choice of stationary and mobile phases, preparation of uniform adsorbent layers, and detection of separated components. TLC is widely used in pharmaceutical analysis, organic synthesis, and biochemical research due to its simplicity, speed, sensitivity, and ability to separate small quantities of substances.
Thin layer chromatography is a technique used to separate mixtures based on differences in how compounds partition between a stationary phase and mobile phase. It involves applying sample spots to a thin silica coated plate then developing in a solvent mobile phase, causing compounds to migrate different distances. The ratio of a compound's migration distance to the solvent front distance is its Rf value, which can identify compounds by comparison to standards. Thin layer chromatography has applications in pharmaceutical analysis, food and cosmetic testing, and biochemical analysis to identify compounds, assess purity, and detect impurities.
Thin layer chromatography- Advances of Paper Chromatographysadia muhammad din
Â
Thin layer chromatography (TLC) is a technique used to separate mixtures by distributing the components between a stationary phase, such as silica gel coated onto a plate, and a mobile phase, such as a solvent that moves across the plate. TLC involves spotting a sample onto the plate, developing it by allowing the mobile phase to travel up the plate, and visualizing the separated components, which travel at different rates depending on how they partition between the phases. TLC is a simple, fast, and inexpensive method used to analyze organic compounds and test purity across various fields like pharmaceuticals, clinical chemistry, and food analysis.
This document provides information on three chromatography techniques: thin layer chromatography (TLC), paper chromatography, and column chromatography. It describes the basic principles, components, procedures, and applications of each technique. TLC involves separating compounds on a thin stationary phase using a mobile phase. Paper chromatography uses paper as the stationary phase. Column chromatography uses a column packed with an adsorbent stationary phase to separate mixtures based on compound affinity. Each technique can be used to analyze and purify mixtures of compounds.
This document provides an overview of chromatography techniques. It begins with a brief history of chromatography from 1903 to 1965. It then discusses the importance of chromatography and its applications in various fields. The document defines chromatography and describes various techniques including thin layer chromatography, gas chromatography, high performance liquid chromatography, and ion exchange chromatography. It explains the theoretical bases and factors affecting chromatographic separation. Finally, it discusses terminology, applications, and detection methods in chromatography.
This document provides information on column chromatography and gas chromatography techniques used in research. Column chromatography separates compounds based on differential rates of movement through an adsorbent column. It involves a stationary phase, mobile phase, and sample mixture. Gas chromatography also separates compounds based on partitioning between a gas mobile phase and stationary phase coating. It outlines the procedure, applications, advantages, and limitations of both techniques.
Chromatography separates components in a mixture using a stationary and mobile phase. High performance liquid chromatography (HPLC) is a type of chromatography that uses high pressure to force a liquid mobile phase through a column packed with solid particles. The document discusses various aspects of HPLC including separation modes, selecting stationary and mobile phases, HPLC system components, and applications.
Chromatography is a technique used to separate mixtures by exploiting differences in how components interact with stationary and mobile phases. The document discusses several types of chromatography including column chromatography, paper chromatography, thin layer chromatography, gas chromatography, and high performance liquid chromatography. It provides details on the principles, instrumentation, and procedures for each type. The key points are that chromatography separates mixtures based on how components partition between stationary and mobile phases, resulting in separation over time. A variety of stationary and mobile phases can be used depending on the application and components.
Similar to THIN LjsjsjsnsjsjsjejsjwjsAYER CROMATOGRAPHY.pptx (20)
ESR spectroscopy in liquid food and beverages.pptxPRIYANKA PATEL
Â
With increasing population, people need to rely on packaged food stuffs. Packaging of food materials requires the preservation of food. There are various methods for the treatment of food to preserve them and irradiation treatment of food is one of them. It is the most common and the most harmless method for the food preservation as it does not alter the necessary micronutrients of food materials. Although irradiated food doesn’t cause any harm to the human health but still the quality assessment of food is required to provide consumers with necessary information about the food. ESR spectroscopy is the most sophisticated way to investigate the quality of the food and the free radicals induced during the processing of the food. ESR spin trapping technique is useful for the detection of highly unstable radicals in the food. The antioxidant capability of liquid food and beverages in mainly performed by spin trapping technique.
BREEDING METHODS FOR DISEASE RESISTANCE.pptxRASHMI M G
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Plant breeding for disease resistance is a strategy to reduce crop losses caused by disease. Plants have an innate immune system that allows them to recognize pathogens and provide resistance. However, breeding for long-lasting resistance often involves combining multiple resistance genes
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
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Slides from talk:
Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/
Nucleophilic Addition of carbonyl compounds.pptxSSR02
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Nucleophilic addition is the most important reaction of carbonyls. Not just aldehydes and ketones, but also carboxylic acid derivatives in general.
Carbonyls undergo addition reactions with a large range of nucleophiles.
Comparing the relative basicity of the nucleophile and the product is extremely helpful in determining how reversible the addition reaction is. Reactions with Grignards and hydrides are irreversible. Reactions with weak bases like halides and carboxylates generally don’t happen.
Electronic effects (inductive effects, electron donation) have a large impact on reactivity.
Large groups adjacent to the carbonyl will slow the rate of reaction.
Neutral nucleophiles can also add to carbonyls, although their additions are generally slower and more reversible. Acid catalysis is sometimes employed to increase the rate of addition.
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
Context. With a mass exceeding several 104 M⊙ and a rich and dense population of massive stars, supermassive young star clusters
represent the most massive star-forming environment that is dominated by the feedback from massive stars and gravitational interactions
among stars.
Aims. In this paper we present the Extended Westerlund 1 and 2 Open Clusters Survey (EWOCS) project, which aims to investigate
the influence of the starburst environment on the formation of stars and planets, and on the evolution of both low and high mass stars.
The primary targets of this project are Westerlund 1 and 2, the closest supermassive star clusters to the Sun.
Methods. The project is based primarily on recent observations conducted with the Chandra and JWST observatories. Specifically,
the Chandra survey of Westerlund 1 consists of 36 new ACIS-I observations, nearly co-pointed, for a total exposure time of 1 Msec.
Additionally, we included 8 archival Chandra/ACIS-S observations. This paper presents the resulting catalog of X-ray sources within
and around Westerlund 1. Sources were detected by combining various existing methods, and photon extraction and source validation
were carried out using the ACIS-Extract software.
Results. The EWOCS X-ray catalog comprises 5963 validated sources out of the 9420 initially provided to ACIS-Extract, reaching a
photon flux threshold of approximately 2 × 10−8 photons cm−2
s
−1
. The X-ray sources exhibit a highly concentrated spatial distribution,
with 1075 sources located within the central 1 arcmin. We have successfully detected X-ray emissions from 126 out of the 166 known
massive stars of the cluster, and we have collected over 71 000 photons from the magnetar CXO J164710.20-455217.
ANAMOLOUS SECONDARY GROWTH IN DICOT ROOTS.pptxRASHMI M G
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Abnormal or anomalous secondary growth in plants. It defines secondary growth as an increase in plant girth due to vascular cambium or cork cambium. Anomalous secondary growth does not follow the normal pattern of a single vascular cambium producing xylem internally and phloem externally.
Current Ms word generated power point presentation covers major details about the micronuclei test. It's significance and assays to conduct it. It is used to detect the micronuclei formation inside the cells of nearly every multicellular organism. It's formation takes place during chromosomal sepration at metaphase.
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptxMAGOTI ERNEST
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Although Artemia has been known to man for centuries, its use as a food for the culture of larval organisms apparently began only in the 1930s, when several investigators found that it made an excellent food for newly hatched fish larvae (Litvinenko et al., 2023). As aquaculture developed in the 1960s and ‘70s, the use of Artemia also became more widespread, due both to its convenience and to its nutritional value for larval organisms (Arenas-Pardo et al., 2024). The fact that Artemia dormant cysts can be stored for long periods in cans, and then used as an off-the-shelf food requiring only 24 h of incubation makes them the most convenient, least labor-intensive, live food available for aquaculture (Sorgeloos & Roubach, 2021). The nutritional value of Artemia, especially for marine organisms, is not constant, but varies both geographically and temporally. During the last decade, however, both the causes of Artemia nutritional variability and methods to improve poorquality Artemia have been identified (Loufi et al., 2024).
Brine shrimp (Artemia spp.) are used in marine aquaculture worldwide. Annually, more than 2,000 metric tons of dry cysts are used for cultivation of fish, crustacean, and shellfish larva. Brine shrimp are important to aquaculture because newly hatched brine shrimp nauplii (larvae) provide a food source for many fish fry (Mozanzadeh et al., 2021). Culture and harvesting of brine shrimp eggs represents another aspect of the aquaculture industry. Nauplii and metanauplii of Artemia, commonly known as brine shrimp, play a crucial role in aquaculture due to their nutritional value and suitability as live feed for many aquatic species, particularly in larval stages (Sorgeloos & Roubach, 2021).
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
2. •Thin Layer Chromatography can be defined as a method of
separation or identification of a mixture of components into
individual components.
• TLC is a form of liquid chromatography consisting of two
phases: A mobile phase (liquid) and A stationary phase
(solid).
• Differences in the interactions between the solutes and
stationary and mobile phases enable separation.
INTRODUCTION
3. PRINCIPLE
• TLC technique involves the distribution of components of a mixture to
be separated between two phases.
• The components of the mixture are partitioned between an
adsorbent (stationary phase), and a solvent (mobile phase).
• Different compounds will have different solubility and adsorption to
the two phases between which they are to be partitioned.
• In TLC separation of the individual substances is based on their
relative affinities towards stationary and mobile phases.
PRINCIPLE
4. • The stationary phase: is a thin layer of adsorbent (usually silica gel or
alumina) coated on a plate.
• • The mobile phase: is a developing liquid which flows through the
stationary phase, carrying the samples with it.
• • Components with more affinity towards stationary phase travels
slower.
• • Components with less affinity towards stationary phase travels
faster.
PRINCIPLE
5. TLC -TECHNIQUE
STEP 1: Preparation of Slurry
• A plastic, glass or aluminum sheet is coated with a thin layer of silica gel
(adsorbent).
• Plates must be dried, activated and stored in desiccator until used. TLC -
TECHNIQUE
STEP 2: Preparation of Tank
• Solvent mixtures should be freshly prepared for analysis.
• Solvent is poured down side of the tank (1.5cm depth).
• Tank is covered with the glass lid and kept for saturation.
STEP 3: Application of Sample (Spot)
• A very small amount of sample (solution) to be analyzed is applied in a small spot
with a capillary tube, ~1cm from the bottom of the TLC plate.
6. • • The TLC is developed in a
chamber which contains
the mobile phase (solvent).
• • When the mobile phase
rises up the plate up by
capillary action, the
components dissolve in the
solvent and move.
TLC -TECHNIQUE
7. • Individual components in the sample move up at different
rates.
• • More polar analytes interact more strongly with the
stationary phase move very slowly up.
• • More nonpolar analytes interact less strongly with the
polar silica gel and more strongly with the less polar mobile
phase move higher up.
• • Once the solvent reaches the top (below ~1-2 cm) of the
TLC sheet the plate is removed from the developing
chamber and position of solvent front is marked.
TLC -TECHNIQUE
8. • The solvent is allowed to evaporate from the TLC sheet.
• As the compound is colorless, it can be visualized by suitable
methods.
i Lipids - Iodine vapors
ii Amino acids - Ninhydrin reagent.
• Also, manganese-activated zinc silicate (fluorescent compound), is
added to the adsorbent that allows the visualization of spots under a
black light (UV254 lamp).
• Once visible, the Rf value of each spot can be determined.
TLC -TECHNIQUE
9. Rf- value
the distance travelled by a molecule on TLC plate is Dm where
as the distance travelled by the solvent is Ds, then the
retardation factor (Rf) of molecule is given by:
10. Technical troubles with thin layer chromatography
1. Tailing effect-In general sample forms round circular spot on the TLC
plate. It is due to the uniform movement of the solvent front through out
the plate. But in few cases instead of forming a spot, a compound forms a
spot with long trail or rocket shape spot .it is due to few reasons as given
below
B. Fluctuation in temp or opening of chamber
A. Over-loading
11. Technical troubles with thin layer chromatography
2. No movement of sample- In few cases, a sample doesn’t move from the spot after
the run is completed. These problems are common with high molecular weight
substances such as protein or chemicals with large number of functional group. In this
case, a change in polarity or pH of solvent system can be explored to bring the
compound into the solvent front so that it run on silica plate to get resolved.
3. Movement is too fast-In few cases, the movement of a compound is too fast and
does not give time to interact with the matrix to resolve into individual compounds. In
this case, a change in polarity of solvent system can be explored to retard the running
of the sample.
12. Applications of Thin layer Chromatography
• TLC is used in qualitative and quantitative analysis to
separate organic compounds and to test the purity of
compounds.
• This technique is useful for separation of lipids, amino acids
and sugars etc.
• It is useful in:
• Identification of components of a mixture.
• Following the course of a reaction,
• Analyzing fractions collected during purification,
• Analyzing the purity of a compound.