This document discusses tissue processing techniques including automated and manual methods. It covers the principles of dehydration, clearing, infiltration and embedding of tissue samples. The key steps are dehydration using ethanol solutions, clearing with xylene, and infiltration and embedding in paraffin wax to produce blocks that can be cut into slides. Automated processors use either tissue or fluid transfer to move samples between solutions, offering faster processing but requiring electricity, while manual methods are slower but more robust. Proper tissue orientation during embedding is also discussed.
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
263778731218 Abortion Clinic /Pills In Harare ,ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group of receptionists, nurses, and physicians have worked together as a teamof receptionists, nurses, and physicians have worked together as a team wwww.lisywomensclinic.co.za/
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
NVBDCP.pptx Nation vector borne disease control programSapna Thakur
NVBDCP was launched in 2003-2004 . Vector-Borne Disease: Disease that results from an infection transmitted to humans and other animals by blood-feeding arthropods, such as mosquitoes, ticks, and fleas. Examples of vector-borne diseases include Dengue fever, West Nile Virus, Lyme disease, and malaria.
HOT NEW PRODUCT! BIG SALES FAST SHIPPING NOW FROM CHINA!! EU KU DB BK substit...GL Anaacs
Contact us if you are interested:
Email / Skype : kefaya1771@gmail.com
Threema: PXHY5PDH
New BATCH Ku !!! MUCH IN DEMAND FAST SALE EVERY BATCH HAPPY GOOD EFFECT BIG BATCH !
Contact me on Threema or skype to start big business!!
Hot-sale products:
NEW HOT EUTYLONE WHITE CRYSTAL!!
5cl-adba precursor (semi finished )
5cl-adba raw materials
ADBB precursor (semi finished )
ADBB raw materials
APVP powder
5fadb/4f-adb
Jwh018 / Jwh210
Eutylone crystal
Protonitazene (hydrochloride) CAS: 119276-01-6
Flubrotizolam CAS: 57801-95-3
Metonitazene CAS: 14680-51-4
Payment terms: Western Union,MoneyGram,Bitcoin or USDT.
Deliver Time: Usually 7-15days
Shipping method: FedEx, TNT, DHL,UPS etc.Our deliveries are 100% safe, fast, reliable and discreet.
Samples will be sent for your evaluation!If you are interested in, please contact me, let's talk details.
We specializes in exporting high quality Research chemical, medical intermediate, Pharmaceutical chemicals and so on. Products are exported to USA, Canada, France, Korea, Japan,Russia, Southeast Asia and other countries.
Knee anatomy and clinical tests 2024.pdfvimalpl1234
This includes all relevant anatomy and clinical tests compiled from standard textbooks, Campbell,netter etc..It is comprehensive and best suited for orthopaedicians and orthopaedic residents.
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
1. Chapter 5:
Automation, Infiltration, and Embedding
Learning Objectives:
1. To be able to understand the histotechnique; in terms of preparation,
sequence, and activities.
2. To be able to discuss the effects of vacuum and heat in automated tissue
processing.
3. To be able to contrast manual and automated tissue processing.
4. To be able to name the two types of automated tissue processors, and discuss
each principles.
5. To be able to know the procedure of changing solutions.
6. To be able to describe and discuss the importance of infiltration or
impregnation.
7. To be able to know the selection of infiltration embedding medium to be used.
8. To be able to know the types of impregnation and embedding media.
2. Learning Objectives:
9. To be able to know the factors qualifying paraffin wax as
the most common and best medium.
10. To be able to know the properties of paraffin wax.
11. To be able to know the melting point of paraffin wax
12. To be able to discuss the problems associated with
infiltration process.
13. To be able to know the ideal properties of infiltration and
embedding media.
14. To be able to describe, discuss the importance, and know
the other names of embedding.
15. To be able to discuss and contrast the process of
embedding frozen sections.
16. To be able to know the importance and procedure for
tissue orientation.
3. Automated Tissue Processor
In modern laboratories - dehydration, clearing and infiltration are all carried
out by automated tissue processor.
It can also perform in accelerated mode (taking a shorter time period) by
subjecting it to vacuum and heat (40 degrees Celcius).
Smaller laboratories in developing countries however, still perform manually.
In the hands of a good technician, the manual procedure is in no
inferior to automated tissue processing but the former is tedious and requires
constant attention. Interruptions in the electricity supply and lack of service
support may cause problems in automated tissue processor.
4. Two broad types of automation processors;
a) tissue transfer
b) fluid transfer.
Tissue transfer
automatic processor
Tissue transfer – A series of stationary vats (containers), arranged in a
circle with a timing device. These vats hold reagents and paraffin
wax. The basket (receptacle) holds the cassettes and automatically
changes position and takes a bath in different reagents kept in vats at
a certain time, in order to accomplish dehydration, clearing and
infiltration.
5. Fluid transfer – fluids are pumped to and from a retort
in which the tissues remain stationary.
Fluid transfer automatic processor
6. Changing of Solutions
The fluids used in complete dehydration and
clearing tend to become contaminated with fluid
carried over from previous vat by the tissue.
After using them for 2 – 3 days, the last
solution in the series (e.g. xylene 2) is replaced by
fresh solution, and the previously used one is moved
forward while the first one (e.g. xylene 1) is discarded.
7. Infiltration (Impregnation)
Infiltration or impregnation is done after dehydration
and clearing.
Tissue must be infiltrated with the supporting
(embedding) medium, in order to hold the cells and intercellular
structures in their proper relationship while sections are being
cut.
The medium used to infiltrate the tissue is usually the
same medium utilized for impregnation.
8. There are generally four types of tissue impregnation and embedding
media, namely;
1. Paraffin wax
2. Celloidin
3. Gelatin
4. Plastic
(L to R) Paraffin pellets with paraffin block,
paraffin pellets
Paraffin wax remains the most common and the best medium because;
1. large numbers of tissue blocks may be process in a short time
2. serial sections are easily obtained, and
3. routine and most special staining can be done easily
9. Paraffin wax properties:
1. Is used for routine embedding
2. Melting point at 56 – 58 degrees Celcius
3. It is a polycrystalline mixture of solid hydrocarbons
produced during the refining of coal and mineral oils.
4. It is about two thirds the density and slightly more elastic
than dried protein.
Inadequate impregnation leads to drying and shrinking of
embedded tissues. In addition, during cutting, cracks and crumbles
develop if tissues are inadequately supported by wax.
On the other hand, excessive exposure to high temperature
(higher than 5 degrees melting point) will over harden the tissues
and will not yield good sections.
10. Ideally the infiltrating and embedding media should be:
1. Soluble in processing fluids
2. Suitable for sectioning and ribboning
3. Molten between 30 - 60 degrees Celcius
4. Translucent or transparent; colorless
5. Stable
6. Homogenous
7. Capable of flattening after ribboning
8. Non-toxic
9. Odorless
10. Easy to handle
11. Inexpensive
11. Embedding (Casting or Blocking)
It is a method of placing the infiltrated tissue in
warm liquid paraffin (embedding medium) that
solidifies into a firm block when it cools down to
room temperature.
This process provides support to the tissue to be
cut on a microtome.
During this process the tissues are placed into a
mold along with embedding medium which is
then hardened by cooling.
13. Embedded Frozen Sections
The frozen sections
In frozen, un-fixed sections, the embedding medium is not
needed.
The water in the tissue is frozen, and as ice produces a firm
block of tissue, the ice acts as the embedding medium.
14. Tissue orientation in mold (L to R) Metal mold, metal mold
with cassette, paraffin block
During embedding, it is important to orient the tissue in a way
that will provide the best information to the pathologist.
Ideally done at the time the pathologist is taking out the “bit or
block” from gross specimen. The pathologist may notch with a
scalpel or mark with india ink the side opposite that is to be cut.