1st summary rise.1st Summary-Genomics: Fish DNA Barcoding & Protein Profiling Workshop & My Life with Worms
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DNA barcoding and protein profiling use electrophoresis techniques to separate molecules like DNA and proteins based on their charge and shape. This reveals information about the number, size, and similarities of proteins or DNA sequences between samples. DNA barcoding extracts, purifies, and amplifies the cytochrome c oxidase 1 gene from DNA to facilitate species identification. Protein profiling identifies and compares proteins from different organisms. After learning about these techniques, the workshop discussed research analyzing taxonomy and genetics of ribbon worms to build an evolutionary tree and better understand biological diversity.
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Genomics: Fish DNA Barcoding & Protein Profiling Workshop
In electrophoresis, molecules like DNA and proteins are separated according to their charge and shape when placed in an electric field. This technique reveals the number, size, and similarities or differences of proteins in samples. DNA electrophoresis involves isolating DNA strands in a lysis solution, preparing a primer mix, amplifying DNA in a thermal cycler, and loading the DNA into agarose gel. Protein electrophoresis identifies proteins and evolutionary relationships among fish by placing proteins in microtubes with buffer, flicking, incubating, heating, and loading them into polyacrylamide gel. An expert then presented research using these techniques to analyze taxonomy and genetics of Nemertia to build a genealogical tree.
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Recombinant DNA is created by combining DNA from two different organisms. There are three main methods to create recombinant DNA - transformation, phage introduction, and non-bacterial transformation. Transformation involves inserting DNA into a vector, which is then taken up by a host cell like E. coli. Recombinant DNA works by the host cell expressing proteins from the recombinant genes. Recombinant DNA technology has important applications including developing better crops, vaccines, and treatments for genetic diseases.
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This document discusses molecular cloning and its applications in food biotechnology. It begins by outlining the basic steps of molecular cloning, which include DNA extraction, electrophoresis, PCR, cloning, and gene expression. DNA extraction involves isolating genomic DNA from cells through disruption of cell membranes and walls. Electrophoresis is used to separate DNA or proteins by their charge, while PCR amplifies specific DNA sequences. Cloning involves cutting a gene, inserting it into a vector like a plasmid, and transforming the vector into bacteria for expression. The document provides examples of cloning vectors and gene expression systems. It concludes by discussing some applications of these molecular techniques, such as detecting pathogens, producing proteins, and screening bioactive isolates.
Biotech 2011-08-recombinant-dna
Foreign DNA and vector DNA are cut with restriction enzymes, leaving sticky ends. The DNA fragments are then ligated together. Host cells take up the recombinant DNA and propagate it. Libraries contain fragments of DNA from a source inserted into vectors. Genomic libraries contain chromosomal DNA fragments, while cDNA libraries contain mRNA sequences converted to DNA. Expression libraries contain cDNAs in vectors allowing protein expression.
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This document discusses recombinant DNA technology and the tools required. It describes the basic steps: 1) selecting and isolating the gene of interest, 2) choosing a suitable vector, 3) inserting the gene into the vector, 4) inserting the recombinant DNA into a host cell, and 5) expressing and multiplying the recombinant DNA. It also lists common tools used, including restriction enzymes, cloning vectors, foreign DNA, host organisms, and linkers. Examples of each tool are provided, along with their functions in recombinant DNA technology.
Molecular medicine presentation
The document discusses molecular medicine and various diagnostic techniques. It covers diagnostics for infectious diseases, which has shifted from examining microbial phenotypes to using nucleic acid techniques like PCR and probes. These DNA-based methods allow detection without culturing and can identify sub-species and drug resistance genes. The document also discusses diagnostics for genetic diseases using techniques like linkage analysis and pedigree analysis to find disease-causing genes. Finally, it outlines gene therapy techniques like germline and somatic cell therapy which aim to cure inherited diseases by providing a correct copy of defective genes.
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Describe the application of DNA profiling in paternity tests and forensic investigations
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This document discusses current trends in molecular biology and biotechnology. It begins by defining biotechnology and explaining its importance in addressing challenges around feeding and clothing the growing global population. It then describes molecular biology as the study of biological processes at the molecular level, including DNA, RNA, protein synthesis and gene regulation. Some applications of molecular biology discussed include research, diagnosis, forensics, gene therapy and drug design. Key cellular components like DNA, RNA and proteins are also explained. Important techniques in molecular biology like PCR, DNA/RNA blotting, gene expression and cloning, microarrays, and RNA interference are summarized. The uses of embryonic and adult stem cells in research and therapy are also covered briefly.
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Genomics: Fish DNA Barcoding & Protein Profiling Workshop
In electrophoresis, molecules like DNA and proteins are separated according to their charge and shape when placed in an electric field. This technique reveals the number, size, and similarities or differences of proteins in samples. DNA electrophoresis involves isolating DNA strands in a lysis solution, preparing a primer mix, amplifying DNA in a thermal cycler, and loading the DNA into agarose gel. Protein electrophoresis identifies proteins and evolutionary relationships among fish by placing proteins in microtubes with buffer, flicking, incubating, heating, and loading them into polyacrylamide gel. An expert then presented research using these techniques to analyze taxonomy and genetics of Nemertia to build a genealogical tree.
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Recombinant DNA is created by combining DNA from two different organisms. There are three main methods to create recombinant DNA - transformation, phage introduction, and non-bacterial transformation. Transformation involves inserting DNA into a vector, which is then taken up by a host cell like E. coli. Recombinant DNA works by the host cell expressing proteins from the recombinant genes. Recombinant DNA technology has important applications including developing better crops, vaccines, and treatments for genetic diseases.
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This document discusses molecular cloning and its applications in food biotechnology. It begins by outlining the basic steps of molecular cloning, which include DNA extraction, electrophoresis, PCR, cloning, and gene expression. DNA extraction involves isolating genomic DNA from cells through disruption of cell membranes and walls. Electrophoresis is used to separate DNA or proteins by their charge, while PCR amplifies specific DNA sequences. Cloning involves cutting a gene, inserting it into a vector like a plasmid, and transforming the vector into bacteria for expression. The document provides examples of cloning vectors and gene expression systems. It concludes by discussing some applications of these molecular techniques, such as detecting pathogens, producing proteins, and screening bioactive isolates.
Biotech 2011-08-recombinant-dna
Foreign DNA and vector DNA are cut with restriction enzymes, leaving sticky ends. The DNA fragments are then ligated together. Host cells take up the recombinant DNA and propagate it. Libraries contain fragments of DNA from a source inserted into vectors. Genomic libraries contain chromosomal DNA fragments, while cDNA libraries contain mRNA sequences converted to DNA. Expression libraries contain cDNAs in vectors allowing protein expression.
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This document discusses recombinant DNA technology and the tools required. It describes the basic steps: 1) selecting and isolating the gene of interest, 2) choosing a suitable vector, 3) inserting the gene into the vector, 4) inserting the recombinant DNA into a host cell, and 5) expressing and multiplying the recombinant DNA. It also lists common tools used, including restriction enzymes, cloning vectors, foreign DNA, host organisms, and linkers. Examples of each tool are provided, along with their functions in recombinant DNA technology.
Molecular medicine presentation
The document discusses molecular medicine and various diagnostic techniques. It covers diagnostics for infectious diseases, which has shifted from examining microbial phenotypes to using nucleic acid techniques like PCR and probes. These DNA-based methods allow detection without culturing and can identify sub-species and drug resistance genes. The document also discusses diagnostics for genetic diseases using techniques like linkage analysis and pedigree analysis to find disease-causing genes. Finally, it outlines gene therapy techniques like germline and somatic cell therapy which aim to cure inherited diseases by providing a correct copy of defective genes.
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This document discusses bacterial genetics. It begins by explaining that bacterial properties are controlled by genes in their chromosomal DNA, which is replicated to maintain traits across generations. While bacteria have no nucleus or nucleolus, their DNA forms the classic double helix structure described by Watson and Crick. Genetic information is stored in codons that code for amino acids. Bacteria can experience phenotypic variation from environment or genotypic variation through mutation or gene transfer via transformation, transduction, conjugation, or cell fusion. Mutation may occur spontaneously or be induced, and can involve changes like base substitutions or frameshifts. Gene transfer allows uptake of external DNA and transmission of genes between bacteria.
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1. Recombinant DNA technology involves manipulating DNA from different species and combining them to form new recombinant DNA molecules.
2. Key steps include using restriction enzymes to cut DNA at specific sites, and DNA ligase to join DNA fragments together into vectors like plasmids.
3. The recombinant DNA can then be replicated in host cells like bacteria to produce multiple copies for analysis.
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1. Biotechnology relies on restriction enzymes that cut DNA at specific nucleotide sequences. Different enzymes cut DNA in different ways, leaving either blunt or sticky ends. Restriction maps show the lengths of DNA fragments cut by these enzymes.
2. The polymerase chain reaction (PCR) amplifies specific DNA sequences. It uses DNA polymerase to copy short DNA segments billions of times over, by cycling between high and low temperatures.
3. DNA fingerprinting identifies individuals by analyzing variations in noncoding DNA regions containing repeating sequences. The probability that any two individuals will have identical fingerprints across multiple regions is very small.
Dna manipulation
Genetic engineering involves manipulating an organism's DNA by inserting DNA from another organism. It is used to increase or decrease expression of specific genes. Restriction enzymes cut DNA at specific sequences, gel electrophoresis separates DNA fragments by size using electric charge, and polymerase chain reaction makes millions of copies of a DNA fragment. Biotechnology uses genetic engineering to solve problems by producing transgenic organisms with genes from another species. Transgenic plants are engineered for pest and virus resistance to increase crop survival.
Bacterial genetics 1 .by Sumia Alfitoury
al-salam alykom ..
this lecture starts with basic definitions in genetic , also talk about DNA & RNA ( structures , types , similarities and differences ) .
it talks about bacterial DNA ( chromosome structure / plamids structure and functions / transponon types )
later , discusses about central dogma / gene expression starting from genetic code/codons , then DNA replication , trancription and finally translation
prepared by Sumia Abdalsalam Alfitoury / Libya
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Recombinant DNA technology involves combining DNA from different sources by using restriction enzymes and plasmids. This allows scientists to [1] cure or treat diseases by introducing therapeutic genes into organisms, [2] genetically modify foods, and [3] better understand genetics. Bacteria are commonly used because they reproduce quickly and it is easy to introduce foreign DNA. The process involves isolating genes, cutting DNA with restriction enzymes, combining DNA fragments, and inserting the new recombinant DNA into bacteria. This can be used to produce human insulin in E. coli for diabetes treatment.
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The document summarizes the central dogma of biology and the discovery of DNA as the genetic material. It describes key experiments that showed DNA replicates in a semiconservative manner, with each parental strand serving as a template for a new complementary daughter strand. The process of DNA replication requires several enzymes including DNA polymerase, helicase, ligase and primase to unwind, copy and join new DNA strands.
Ap Bio Ch 13 Power Point
The document discusses biotechnology and its traditional and modern applications. It summarizes that biotechnology has traditionally involved techniques like using yeast to make beer/wine and selective breeding of plants and animals. Modern biotechnology focuses on genetic engineering using recombinant DNA technology to modify genes and achieve goals like understanding disease and improving agriculture. It also discusses techniques like polymerase chain reaction (PCR) and gel electrophoresis that are used in biotechnology and forensics.
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This document discusses the structure and replication of DNA. It provides details on:
1. The key components of DNA including deoxyribose sugar, phosphate groups, and nitrogenous bases. It also describes how nucleotides and polynucleotide strands are formed.
2. The double helix structure of DNA proposed by Watson and Crick, including how the strands are antiparallel and connected through complementary base pairing.
3. Semiconservative replication of DNA, where each old DNA strand acts as a template for a new complementary strand, resulting in two identical double-stranded DNA molecules after replication.
4. Experiments by Meselson and Stahl using isotopes that provided evidence supporting the
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Genetic engineering allows manipulation of a genome by introducing, eliminating, or modifying genes. The main steps are: 1) Isolating the gene of interest using restriction enzymes to cut DNA into fragments; 2) Inserting the gene into a bacterial plasmid using recombinant DNA techniques; 3) Detecting colonies containing the inserted gene through tests or hybridization; 4) Cloning the bacteria to produce many copies containing the gene.
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This document provides an overview of genetic engineering and its applications to microorganisms. It defines genetic engineering as the direct manipulation of an organism's genome using biotechnology. The key steps involved are isolating the gene of interest, inserting it into a vector, introducing the vector into a host cell, and harvesting the gene product from the clone. Common hosts used are bacteria, yeast, plant and animal cells. The document also discusses some tools used in genetic engineering like restriction enzymes and DNA ligase. It outlines several applications of genetic engineering in medicine, research, agriculture and industry. It concludes by noting some ethical and safety concerns regarding genetically modified organisms.
principle and applications of recombinant DNA technology
Recombinant DNA technology involves combining DNA sequences from different species that would not normally occur together to create artificial DNA and alter the genetics of living cells. There are three main methods to create recombinant DNA - transformation, phage introduction, and non-bacterial transformation. Transformation involves selecting a DNA fragment, inserting it into a vector, and introducing the vector into a host cell like E. coli. Recombinant DNA technology has many applications, including producing proteins and hormones, disease diagnosis and treatment, genetically engineering plants, and forensic analysis.
Hershey and chase
The Hershey-Chase experiment helped confirm that DNA is the genetic material by showing that the DNA, but not the proteins, of bacteriophages enters host bacterial cells during infection. They uniquely labeled the DNA and proteins of bacteriophages with different radioactive isotopes, then showed that the progeny of infected bacteria contained the radioactivity from the original phage DNA but not proteins. This provided strong evidence that DNA, rather than protein, carries the genetic information required for reproduction.
Bacterial genetics png 2011
1. Bacterial genetics involves the study of heredity and variation in bacteria. Genes are segments of DNA that code for polypeptides.
2. DNA is the genetic material that is replicated and passed to offspring. RNA is involved in transcription and translation of genes into proteins.
3. Mutation, transformation, transduction, conjugation and lysogenic conversion are mechanisms by which bacteria exchange genetic material and acquire new traits. This allows for genetic variation and evolution of bacteria.
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Techniques to study genes include polymerase chain reaction (PCR) to amplify DNA fragments, using restriction enzymes to cut DNA at specific sequences, electrophoresis to separate DNA fragments by size, and DNA probes to find specific sequences. Genetic engineering techniques allow genes to be inserted into bacteria using vectors like plasmids. This allows production of proteins like human insulin. Golden rice has been genetically engineered to produce beta-carotene to reduce vitamin A deficiency in some countries. However, it has also raised ethical concerns about reducing biodiversity.
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Recombinant DNA technology uses restriction enzymes and DNA ligase to cut and join DNA fragments from different sources to construct recombinant DNA molecules. This technique was discovered in the 1970s and has since been used to develop transgenic plants with improved traits like higher yield, increased stress and pest resistance, and the ability to produce valuable pharmaceuticals. Some key applications include producing human insulin and anemia treatments, developing herbicide and insect resistant crop varieties, and engineering disease resistance in plants. Recombinant DNA technology is now widely used in agriculture and has contributed to over 70% of foods in supermarkets coming from genetically modified crops.
Genetic engineering
genetic engineering: Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. Many organism are manipulated with the help genetic engineering useful for mankind.
THE GENETIC MATERIAL
GENETIC MATERIAL refers to the material of which genes are made up of. It includes both DNA and RNA. Though in most of the organism DNA is playing this role, but in certain viruses RNA is storing all the genetic information of the individual. Here we are discussing about the discovery and property of these genetic material.
Gene to protein paragraph
The document summarizes a workshop that taught how proteins are formed in the body from DNA. DNA contains genetic codes that determine the structure and function of proteins and other cellular components. During the workshop, students extracted DNA from cheek cells and used techniques like PCR and electrophoresis to replicate and separate DNA, demonstrating how specific genes and proteins are identified. These techniques have various applications in forensics, research, genetic engineering, and paleontology.
Microbiology Ch 07 lecture_presentation
This document summarizes the structure and replication of genomes. It discusses the structure of prokaryotic and eukaryotic genomes, including bacterial chromosomes, plasmids, and eukaryotic nuclear and extranuclear DNA. It also describes DNA replication as semiconservative and requiring triphosphate deoxyribonucleotides. Key steps in DNA replication include initiation at the origin, replication by DNA polymerase in the 5' to 3' direction, and discontinuous lagging strand synthesis using Okazaki fragments.
Final dna summary
DNA barcoding uses mitochondrial DNA to identify unknown species by comparing DNA sequences to a reference database of known species. The workshop demonstrated DNA barcoding techniques like extracting and sequencing mitochondrial DNA from fish samples. While a useful identification tool, DNA barcoding must be supplemented with taxonomic observation to avoid misclassification, as two species can share the same mitochondrial DNA. The workshop also covered proteomics to identify active enzymes in samples and construct evolutionary trees, helping classify species. Participants learned about DNA barcoding, proteomics, and one researcher's work identifying rare worm species.
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Bacterial genetics
This document discusses bacterial genetics. It begins by explaining that bacterial properties are controlled by genes in their chromosomal DNA, which is replicated to maintain traits across generations. While bacteria have no nucleus or nucleolus, their DNA forms the classic double helix structure described by Watson and Crick. Genetic information is stored in codons that code for amino acids. Bacteria can experience phenotypic variation from environment or genotypic variation through mutation or gene transfer via transformation, transduction, conjugation, or cell fusion. Mutation may occur spontaneously or be induced, and can involve changes like base substitutions or frameshifts. Gene transfer allows uptake of external DNA and transmission of genes between bacteria.
Lecture 11 Recombinant Dna Tech
1. Recombinant DNA technology involves manipulating DNA from different species and combining them to form new recombinant DNA molecules.
2. Key steps include using restriction enzymes to cut DNA at specific sites, and DNA ligase to join DNA fragments together into vectors like plasmids.
3. The recombinant DNA can then be replicated in host cells like bacteria to produce multiple copies for analysis.
Unit 9 Manipulating Dna
1. Biotechnology relies on restriction enzymes that cut DNA at specific nucleotide sequences. Different enzymes cut DNA in different ways, leaving either blunt or sticky ends. Restriction maps show the lengths of DNA fragments cut by these enzymes.
2. The polymerase chain reaction (PCR) amplifies specific DNA sequences. It uses DNA polymerase to copy short DNA segments billions of times over, by cycling between high and low temperatures.
3. DNA fingerprinting identifies individuals by analyzing variations in noncoding DNA regions containing repeating sequences. The probability that any two individuals will have identical fingerprints across multiple regions is very small.
Dna manipulation
Genetic engineering involves manipulating an organism's DNA by inserting DNA from another organism. It is used to increase or decrease expression of specific genes. Restriction enzymes cut DNA at specific sequences, gel electrophoresis separates DNA fragments by size using electric charge, and polymerase chain reaction makes millions of copies of a DNA fragment. Biotechnology uses genetic engineering to solve problems by producing transgenic organisms with genes from another species. Transgenic plants are engineered for pest and virus resistance to increase crop survival.
Bacterial genetics 1 .by Sumia Alfitoury
al-salam alykom ..
this lecture starts with basic definitions in genetic , also talk about DNA & RNA ( structures , types , similarities and differences ) .
it talks about bacterial DNA ( chromosome structure / plamids structure and functions / transponon types )
later , discusses about central dogma / gene expression starting from genetic code/codons , then DNA replication , trancription and finally translation
prepared by Sumia Abdalsalam Alfitoury / Libya
Recombinant dna technology
Recombinant DNA technology involves combining DNA from different sources by using restriction enzymes and plasmids. This allows scientists to [1] cure or treat diseases by introducing therapeutic genes into organisms, [2] genetically modify foods, and [3] better understand genetics. Bacteria are commonly used because they reproduce quickly and it is easy to introduce foreign DNA. The process involves isolating genes, cutting DNA with restriction enzymes, combining DNA fragments, and inserting the new recombinant DNA into bacteria. This can be used to produce human insulin in E. coli for diabetes treatment.
Molecular Genetics
The document summarizes the central dogma of biology and the discovery of DNA as the genetic material. It describes key experiments that showed DNA replicates in a semiconservative manner, with each parental strand serving as a template for a new complementary daughter strand. The process of DNA replication requires several enzymes including DNA polymerase, helicase, ligase and primase to unwind, copy and join new DNA strands.
Ap Bio Ch 13 Power Point
The document discusses biotechnology and its traditional and modern applications. It summarizes that biotechnology has traditionally involved techniques like using yeast to make beer/wine and selective breeding of plants and animals. Modern biotechnology focuses on genetic engineering using recombinant DNA technology to modify genes and achieve goals like understanding disease and improving agriculture. It also discusses techniques like polymerase chain reaction (PCR) and gel electrophoresis that are used in biotechnology and forensics.
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This document discusses the structure and replication of DNA. It provides details on:
1. The key components of DNA including deoxyribose sugar, phosphate groups, and nitrogenous bases. It also describes how nucleotides and polynucleotide strands are formed.
2. The double helix structure of DNA proposed by Watson and Crick, including how the strands are antiparallel and connected through complementary base pairing.
3. Semiconservative replication of DNA, where each old DNA strand acts as a template for a new complementary strand, resulting in two identical double-stranded DNA molecules after replication.
4. Experiments by Meselson and Stahl using isotopes that provided evidence supporting the
The process of genetic engineering in detail
Genetic engineering allows manipulation of a genome by introducing, eliminating, or modifying genes. The main steps are: 1) Isolating the gene of interest using restriction enzymes to cut DNA into fragments; 2) Inserting the gene into a bacterial plasmid using recombinant DNA techniques; 3) Detecting colonies containing the inserted gene through tests or hybridization; 4) Cloning the bacteria to produce many copies containing the gene.
Microbial genetics lectures 22,23, and 24
This document provides an overview of genetic engineering and its applications to microorganisms. It defines genetic engineering as the direct manipulation of an organism's genome using biotechnology. The key steps involved are isolating the gene of interest, inserting it into a vector, introducing the vector into a host cell, and harvesting the gene product from the clone. Common hosts used are bacteria, yeast, plant and animal cells. The document also discusses some tools used in genetic engineering like restriction enzymes and DNA ligase. It outlines several applications of genetic engineering in medicine, research, agriculture and industry. It concludes by noting some ethical and safety concerns regarding genetically modified organisms.
principle and applications of recombinant DNA technology
Recombinant DNA technology involves combining DNA sequences from different species that would not normally occur together to create artificial DNA and alter the genetics of living cells. There are three main methods to create recombinant DNA - transformation, phage introduction, and non-bacterial transformation. Transformation involves selecting a DNA fragment, inserting it into a vector, and introducing the vector into a host cell like E. coli. Recombinant DNA technology has many applications, including producing proteins and hormones, disease diagnosis and treatment, genetically engineering plants, and forensic analysis.
Hershey and chase
The Hershey-Chase experiment helped confirm that DNA is the genetic material by showing that the DNA, but not the proteins, of bacteriophages enters host bacterial cells during infection. They uniquely labeled the DNA and proteins of bacteriophages with different radioactive isotopes, then showed that the progeny of infected bacteria contained the radioactivity from the original phage DNA but not proteins. This provided strong evidence that DNA, rather than protein, carries the genetic information required for reproduction.
Bacterial genetics png 2011
1. Bacterial genetics involves the study of heredity and variation in bacteria. Genes are segments of DNA that code for polypeptides.
2. DNA is the genetic material that is replicated and passed to offspring. RNA is involved in transcription and translation of genes into proteins.
3. Mutation, transformation, transduction, conjugation and lysogenic conversion are mechanisms by which bacteria exchange genetic material and acquire new traits. This allows for genetic variation and evolution of bacteria.
Gene Technologies
Techniques to study genes include polymerase chain reaction (PCR) to amplify DNA fragments, using restriction enzymes to cut DNA at specific sequences, electrophoresis to separate DNA fragments by size, and DNA probes to find specific sequences. Genetic engineering techniques allow genes to be inserted into bacteria using vectors like plasmids. This allows production of proteins like human insulin. Golden rice has been genetically engineered to produce beta-carotene to reduce vitamin A deficiency in some countries. However, it has also raised ethical concerns about reducing biodiversity.
Role of r dna technology
Recombinant DNA technology uses restriction enzymes and DNA ligase to cut and join DNA fragments from different sources to construct recombinant DNA molecules. This technique was discovered in the 1970s and has since been used to develop transgenic plants with improved traits like higher yield, increased stress and pest resistance, and the ability to produce valuable pharmaceuticals. Some key applications include producing human insulin and anemia treatments, developing herbicide and insect resistant crop varieties, and engineering disease resistance in plants. Recombinant DNA technology is now widely used in agriculture and has contributed to over 70% of foods in supermarkets coming from genetically modified crops.
Genetic engineering
genetic engineering: Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. Many organism are manipulated with the help genetic engineering useful for mankind.
THE GENETIC MATERIAL
GENETIC MATERIAL refers to the material of which genes are made up of. It includes both DNA and RNA. Though in most of the organism DNA is playing this role, but in certain viruses RNA is storing all the genetic information of the individual. Here we are discussing about the discovery and property of these genetic material.
Gene to protein paragraph
The document summarizes a workshop that taught how proteins are formed in the body from DNA. DNA contains genetic codes that determine the structure and function of proteins and other cellular components. During the workshop, students extracted DNA from cheek cells and used techniques like PCR and electrophoresis to replicate and separate DNA, demonstrating how specific genes and proteins are identified. These techniques have various applications in forensics, research, genetic engineering, and paleontology.
Microbiology Ch 07 lecture_presentation
This document summarizes the structure and replication of genomes. It discusses the structure of prokaryotic and eukaryotic genomes, including bacterial chromosomes, plasmids, and eukaryotic nuclear and extranuclear DNA. It also describes DNA replication as semiconservative and requiring triphosphate deoxyribonucleotides. Key steps in DNA replication include initiation at the origin, replication by DNA polymerase in the 5' to 3' direction, and discontinuous lagging strand synthesis using Okazaki fragments.
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principle and applications of recombinant DNA technology
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Final dna summary
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In this workshop, students learned about DNA, genes, proteins, and bacteria through a series of experiments. First, each student isolated their own DNA from saliva samples, which varied in size between students. Next, students used PCR and electrophoresis to copy and study a specific gene. Then, SDS-PAGE electrophoresis was used to separate different sized proteins on a gel. Finally, macrophages were observed consuming GFP-infected E. coli bacteria under a microscope using UV and Lux light to view the fluorescence. The overall workshop covered the process from DNA to proteins and how bacteria function.
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In this workshop, students learned about DNA, genes, proteins, and bacteria through a series of experiments. First, each student isolated their own DNA from saliva samples, which varied in size between students. Next, students used PCR and electrophoresis to copy and study a specific gene. Then, SDS-PAGE electrophoresis was used to separate different sized proteins on a gel. Finally, macrophages were observed consuming GFP-infected E. coli bacteria under a microscope using UV and Lux light to view the fluorescence. The overall workshop covered the process from DNA to proteins and how bacteria function.
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In this laboratory workshop, students learned about DNA, genes, proteins, and bacteria through a series of experiments. First, each student isolated their own DNA from saliva samples, which varied in size between students. Next, students used PCR and electrophoresis to amplify and study a specific gene. Then, SDS-PAGE electrophoresis was used to separate different sized proteins on a gel. Finally, macrophages were observed consuming GFP-labeled E. coli bacteria under a microscope to demonstrate how macrophages fight pathogens. The overall workshop covered the process from DNA to proteins and bacterial infection.
Paragraph 4 rise
In this workshop, students learned about DNA, genes, proteins, and bacteria through a series of experiments. First, each student isolated their own DNA from saliva samples, which varied in size between students. Next, students used PCR and electrophoresis to copy and study a specific gene. Then, SDS-PAGE electrophoresis was used to separate different sized proteins on a gel. Finally, macrophages were observed consuming GFP-infected E. coli bacteria under a microscope using UV and Lux light to view the bacteria. The overall workshop covered how DNA, genes, bacteria, and proteins work.
PCR
PCR is a technique used to exponentially amplify a specific DNA sequence using DNA polymerase. It was invented in 1983 by Kary Mullis and has revolutionized molecular biology. PCR uses DNA polymerase, primers, dNTPs, and thermal cycling to amplify the target DNA sequence. It has numerous applications including disease detection, genetic fingerprinting, sequencing, and classification of organisms.
Southern Blot
Southern blotting is a technique used to detect specific DNA sequences within DNA samples. It involves digesting DNA with restriction enzymes, separating fragments via gel electrophoresis, transferring DNA fragments to a membrane, and probing the membrane with a labeled complementary DNA sequence. Any hybridized probes will be detected, indicating the presence and size of the DNA fragment of interest.
Lydia Yeshitla, Research Scholar at the Neurobiology Section of UCSD
1) The document describes an experiment cloning a pH-sensitive fluorescent protein (pHRed) onto the GLUA1 AMPA receptor subunit to track intracellular trafficking and degradation of AMPA receptors by lysosomes.
2) Restriction enzymes (AGE1 and BSRG1) were used to cut the DNA in order to ligate pHRed onto GLUA1 using PCR. This would allow detection of AMPA receptors in the acidic lysosome lumen.
3) Bacteria were transformed with the ligated pHRed-GluA1 DNA. Colonies were selected and the DNA was sequenced to validate that the cloning procedure was done correctly.
Algae lab report
Through genetic barcoding of the tufA gene, an unknown green algae specimen was identified as belonging to the Ulva compressa clade. DNA was isolated from the specimen and the tufA gene was amplified via PCR. The gene was cloned and the nucleotide sequence analyzed and compared to sequences in GenBank. Phylogenetic analysis indicated the specimen was most similar to Ulva sp. BER-2007, but could not be identified to the species level. While in the U. compressa clade, further testing is needed to confirm its identity as a potential new species of Ulva in Narragansett Bay.
Paragraph 4 rise
In this workshop, students conducted a series of experiments to learn about DNA, genes, proteins, and how macrophages function. First, each student isolated their own DNA from cells in their mouth, which varied in size between students. Next, students used PCR and electrophoresis to study a specific gene and protein. Then, SDS-PAGE electrophoresis was used to separate different sized proteins on a gel. Finally, macrophages were observed consuming GFP-infected E. coli bacteria under a stereomicroscope and UV light, demonstrating their function. The overall goal of the workshop was to understand how DNA, genes, bacteria, and proteins work.
Similar to 1st summary rise.1st Summary-Genomics: Fish DNA Barcoding & Protein Profiling Workshop & My Life with Worms (20)
2017 summer high school textbook (molecular biology and neuroscience)
2017 summer high school textbook (molecular biology and neuroscience)
Lydia Yeshitla, Research Scholar at the Neurobiology Section of UCSD
Lydia Yeshitla, Research Scholar at the Neurobiology Section of UCSD
More from felixjvalles
Reflective essay.docx 2
The student joined the Research Initiative for Scientific Enhancement (RISE) program at the University of Puerto Rico at Cayey to take courses that would help develop their skills as a scientist. Through workshops, seminars, and working with professors on investigations, the student learned various lab techniques in areas like biochemistry and genetics. This included skills like polyacrylamide gel electrophoresis and working with different microscopes. The student also gained experience writing scientific manuscripts and presenting research. Overall, the RISE program helped the student improve their research abilities and prepare for a future career in science.
Language workshop
College students often feel stressed due to assignments, tests, and homework. They find it helpful to talk to other college students about their problems and experiences. Speaking with fellow students allows them to feel less alone and more connected, since they are likely experiencing similar issues. Sharing thoughts and perspectives about classes and professors can help relax both students by confirming they are not the only ones with certain feelings or going through certain experiences. Communicating over their shared experience of being a college student helps them feel more comfortable and entertained.
Language workshop mar 7, 2014.htm
Silver Screen movies have received more positive reviews and comments over the past year, but fewer people have attended their recent films. The author argues that Silver Screen needs to improve their publicity efforts to make more people aware of their well-reviewed movies, rather than focusing solely on production, as their problem lies in advertising the availability of these films rather than the films themselves. However, the analysis does not account for people who disliked the films not commenting or the possibility that competitors may be producing more popular movies.
Language workshop feb 6, 2014.htm
The author describes how their perspective on their mother changed after talking to their older brother. They used to argue with their mother frequently when she would pick them up from school or in public places. However, after their brother advised them to appreciate what their mother does for them and treat her with respect, the author realized their mother's actions came from a place of caring for them. Since that conversation, the author has changed their attitude and no longer argues as much with their mother, understanding she wants the best for them.
Language workshop feb 6, 2014.htm
The author describes how their perspective on their mother changed after talking to their older brother. They used to argue frequently with their mother and disrespect her behavior, finding her annoying. However, their brother advised them to appreciate what their mother does for them and treat her with respect, as she loves them most. This advice from their role model brother made the author realize their mother's actions came from caring for them. Since then, the author has changed their attitude and no longer argues as much with their mother, understanding she aims to provide a good life and education.
Reflective essay
1) Many universities offer scientific programs to promote student research and provide financial aid. The Research Initiative for Scientific Enhancement (RISE) program at the University of Puerto Rico in Cayey does this.
2) Through RISE, the author took courses to develop scientific writing skills like preparing annotated bibliographies and seminar reflections. Students attended seminars on research topics and discussed goals with presenters.
3) Assignments included applying skills from class to write scientific works. With practice, the author improved at writing reflections and bibliographies, learning research and writing techniques.
Carbon dots poem rise
Carbon dots show different properties when isolated from mixtures through fractionation techniques like high performance liquid chromatography (HPLC). When separated, individual carbon dots can express luminescence and have applications in areas like absorption and emission. This separation also reduces their toxicity, making carbon dots more biologically compatible. HPLC is able to separate carbon dots based on their polar interactions with solvents and adsorbents in the column.
Carbon dots poem rise
Carbon dots exhibit useful properties like absorption and emission, but within mixtures these properties cannot be expressed. However, when carbon dots are isolated through high performance liquid chromatography fractionation, which separates components by their polar interactions as solvent is pumped through an adsorbent-filled column, their true abilities are revealed. This allows the now isolated carbon dots to luminesce and display reduced toxicity and greater biological compatibility.
Creative writting rise
In 2549, Professor Morty and his nephew Rick discover a map leading to one of Leonardo Da Vinci's lost inventions. They follow clues from Da Vinci's paintings and writings to uncover a secret workshop under Rome's Fountain of Trevi, filled with Da Vinci's inventions. A robot named St. James is unearthed and refuses to reveal Da Vinci's greatest secret. Accidentally, Rick activates a machine combining the inventions into a spacecraft. This takes them to the advanced planet Vinci, where they meet the real Da Vinci and learn the secret of time travel.
Reflective essay rise
The RISE program at the University of Puerto Rico at Cayey has helped the author develop scientific skills through various courses. These courses included a basic biology lab, seminars on scientific literature techniques, and advanced lab techniques. They learned how to write literature reviews, manuscripts, and participate in research with professors. These experiences improved the author's writing and lab skills as a young scientist. Similar university programs also help undergraduates strengthen their research abilities.
5th summary rise slideshare
Magnetic nanoparticles are used in affinity chromatography to efficiently separate proteins. Proteins play key roles in living systems and isolating pure proteins is important for various applications. Magnetic nanoparticles are more effective than other techniques due to their high surface area and ability to be easily separated. Tissue plasminogen activator (tPA) was used as an example, where magnetic nanoparticles were bound to tPA and several washes were performed to remove non-specifically bound proteins. An elution step lowered the pH to separate tPA from the nanoparticles, resulting in pure proteins. Analysis showed large amounts of protein in the initial and wash samples, but none in the eluates, indicating successful separation of pure tPA using this magnetic nanoparticle technique
5th summary rise slideshare
Magnetic nanoparticles are used in affinity chromatography to efficiently separate proteins. Proteins play important roles in the body but require isolation for various purposes. Magnetic nanoparticles allow for fast, scalable, and automated separation of a target protein from others in a single step. Specifically, the document discusses how magnetic nanoparticles were used to isolate tissue plasminogen activator, an enzyme involved in breaking down blood clots. The nanoparticles were bound to the target protein and then several washes removed nonspecifically bound proteins. An elution step released the pure target protein. Analysis found the target protein was present in initial and waste samples but purified in the eluate, demonstrating the method's effectiveness.
6th summary rise slideshare
Membranes selectively allow the passage of certain components while retaining others due to selective transport mechanisms. An example is osmosis, where solvent molecules pass through a partially permeable membrane to equalize solute concentrations. The purpose of this lab was to investigate how lipid membrane composition affects osmosis and selective transport rates using droplet interface bilayers. Water passed by osmosis between vesicles with different solute concentrations, and selective permeability was observed as dye molecules passed through some membranes but not polymers. Transport rates depended on membrane composition as observed with osmolytes and dyes inserted into lipid bilayers.
4rth summary rise slideshare
Coliphages are bacteriophages that infect E. coli and are used as indicators of fecal pollution in water samples. The objective of this investigation was to examine water samples using the coliphage method to test for animal fecal pollution from different areas and to compare treated versus untreated water. This method is more efficient than other indicators as it is fast, does not require confirmation tests, and is cost-effective. E. coli are used to detect the presence of coliphages. Samples were incubated for 24 hours and showed no growth, indicating no fecal contamination or coliphages were present, resulting in negative tests.
4.monica 13.felix phages report corrected
This document describes an experiment to isolate and characterize mycobacteriophages from soil samples in Puerto Rico. Soil samples were enriched with hosts Mycobacterium smegmatis or Bacillus cereus, and the enriched samples were filtered and plated. A mycobacteriophage, named Musamodel, was discovered in the seventh soil sample from Caguas, Puerto Rico. The isolated phage was purified four times and appears to have a lytic life cycle. While the investigation was not fully completed, it demonstrates that mycobacteriophages can be isolated from Puerto Rican soils.
Mn ps manuscript
This document summarizes an experiment to isolate tissue plasminogen activator (tPA) from HeLa cell culture broth using magnetic nanoparticles (MNPs). tPA helps break down blood clots and has applications in thrombolytic therapy. The experiment involved binding tPA to MNPs, washing away unbound proteins, then eluting tPA from the MNPs. Absorbance measurements and assays showed tPA was present in the load and spent samples but concentrations were low in the eluates. SDS-PAGE and zymography gels confirmed tPA activity in the load and spent but not eluates, possibly due to low concentrations or protein denaturation during elution. The experiment demonstrated the potential
Mn ps final oral presentaion rise corrected
The document discusses using magnetic nanoparticles to separate proteins. It describes isolating tissue plasminogen activator (tPA) from mammalian cell culture broth using magnetic nanoparticles coated with antibodies against tPA. SDS-PAGE and fibrin zymography analyses showed tPA enzymatic activity in the first elution fraction, indicating successful separation of tPA from other proteins using magnetic nanoparticles. The summary concludes magnetic nanoparticles provide an efficient and reusable method for protein separation.
Mn ps final oral presentaion rise corrected
This document summarizes a study that isolated the protein tissue plasminogen activator (tPA) from mammalian cell culture broth using magnetic nanoparticles. SDS-PAGE and fibrin zymography analyses showed that tPA enzyme activity was detected in Eluate 1. Magnetic nanoparticles proved to be an efficient method for separating and isolating tPA due to their ability to rapidly separate proteins from solution. The isolated tPA has applications as a thrombolytic agent to break down blood clots.
Mn ps final oral presentaion rise corrected
Magnetic nanoparticles were used to isolate plasminogen activator (PA) from mammalian cell culture broth. Zymography and SDS-PAGE analysis showed PA enzyme activity and protein presence in the first elution fraction, indicating successful isolation of PA from the culture broth using magnetic nanoparticles. Magnetic nanoparticles provide an efficient and reusable method for protein separation.
Curriculum vitae rise
Felix Valles is a student at the University of Puerto Rico at Cayey pursuing a Bachelor's degree in Biology with the goal of attending veterinary school. He has gained research experience through the RISE program where he has presented papers on topics such as the inhibition of TNF-α and extra-pair paternity in birds. Valles also volunteered at a veterinary hospital in Ponce gaining 57 hours of community service experience. He is fluent in Spanish and English and is a member of the RISE program and Jasperi Pre-vet Group.
More from felixjvalles (20)
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Sri Guru Hargobind Ji - Bandi Chor Guru.pdf
Sri Guru Hargobind Ji (19 June 1595 - 3 March 1644) is revered as the Sixth Nanak.
• On 25 May 1606 Guru Arjan nominated his son Sri Hargobind Ji as his successor. Shortly
afterwards, Guru Arjan was arrested, tortured and killed by order of the Mogul Emperor
Jahangir.
• Guru Hargobind's succession ceremony took place on 24 June 1606. He was barely
eleven years old when he became 6th Guru.
• As ordered by Guru Arjan Dev Ji, he put on two swords, one indicated his spiritual
authority (PIRI) and the other, his temporal authority (MIRI). He thus for the first time
initiated military tradition in the Sikh faith to resist religious persecution, protect
people’s freedom and independence to practice religion by choice. He transformed
Sikhs to be Saints and Soldier.
• He had a long tenure as Guru, lasting 37 years, 9 months and 3 days
OOPS_Lab_Manual - programs using C++ programming language
This manual contains programs on object oriented programming concepts using C++ language.
Determination of Equivalent Circuit parameters and performance characteristic...
Includes the testing of induction motor to draw the circle diagram of induction motor with step wise procedure and calculation for the same. Also explains the working and application of Induction generator
A high-Speed Communication System is based on the Design of a Bi-NoC Router, ...
The Network on Chip (NoC) has emerged as an effective
solution for intercommunication infrastructure within System on
Chip (SoC) designs, overcoming the limitations of traditional
methods that face significant bottlenecks. However, the complexity
of NoC design presents numerous challenges related to
performance metrics such as scalability, latency, power
consumption, and signal integrity. This project addresses the
issues within the router's memory unit and proposes an enhanced
memory structure. To achieve efficient data transfer, FIFO buffers
are implemented in distributed RAM and virtual channels for
FPGA-based NoC. The project introduces advanced FIFO-based
memory units within the NoC router, assessing their performance
in a Bi-directional NoC (Bi-NoC) configuration. The primary
objective is to reduce the router's workload while enhancing the
FIFO internal structure. To further improve data transfer speed,
a Bi-NoC with a self-configurable intercommunication channel is
suggested. Simulation and synthesis results demonstrate
guaranteed throughput, predictable latency, and equitable
network access, showing significant improvement over previous
designs
Object Oriented Analysis and Design - OOAD
This ppt gives detailed description of Object Oriented Analysis and design.
Null Bangalore | Pentesters Approach to AWS IAM
#Abstract:
- Learn more about the real-world methods for auditing AWS IAM (Identity and Access Management) as a pentester. So let us proceed with a brief discussion of IAM as well as some typical misconfigurations and their potential exploits in order to reinforce the understanding of IAM security best practices.
- Gain actionable insights into AWS IAM policies and roles, using hands on approach.
#Prerequisites:
- Basic understanding of AWS services and architecture
- Familiarity with cloud security concepts
- Experience using the AWS Management Console or AWS CLI.
- For hands on lab create account on [killercoda.com](https://killercoda.com/cloudsecurity-scenario/)
# Scenario Covered:
- Basics of IAM in AWS
- Implementing IAM Policies with Least Privilege to Manage S3 Bucket
- Objective: Create an S3 bucket with least privilege IAM policy and validate access.
- Steps:
- Create S3 bucket.
- Attach least privilege policy to IAM user.
- Validate access.
- Exploiting IAM PassRole Misconfiguration
-Allows a user to pass a specific IAM role to an AWS service (ec2), typically used for service access delegation. Then exploit PassRole Misconfiguration granting unauthorized access to sensitive resources.
- Objective: Demonstrate how a PassRole misconfiguration can grant unauthorized access.
- Steps:
- Allow user to pass IAM role to EC2.
- Exploit misconfiguration for unauthorized access.
- Access sensitive resources.
- Exploiting IAM AssumeRole Misconfiguration with Overly Permissive Role
- An overly permissive IAM role configuration can lead to privilege escalation by creating a role with administrative privileges and allow a user to assume this role.
- Objective: Show how overly permissive IAM roles can lead to privilege escalation.
- Steps:
- Create role with administrative privileges.
- Allow user to assume the role.
- Perform administrative actions.
- Differentiation between PassRole vs AssumeRole
Try at [killercoda.com](https://killercoda.com/cloudsecurity-scenario/)
Applications of artificial Intelligence in Mechanical Engineering.pdf
Historically, mechanical engineering has relied heavily on human expertise and empirical methods to solve complex problems. With the introduction of computer-aided design (CAD) and finite element analysis (FEA), the field took its first steps towards digitization. These tools allowed engineers to simulate and analyze mechanical systems with greater accuracy and efficiency. However, the sheer volume of data generated by modern engineering systems and the increasing complexity of these systems have necessitated more advanced analytical tools, paving the way for AI.
AI offers the capability to process vast amounts of data, identify patterns, and make predictions with a level of speed and accuracy unattainable by traditional methods. This has profound implications for mechanical engineering, enabling more efficient design processes, predictive maintenance strategies, and optimized manufacturing operations. AI-driven tools can learn from historical data, adapt to new information, and continuously improve their performance, making them invaluable in tackling the multifaceted challenges of modern mechanical engineering.
一比一原版(USF毕业证)旧金山大学毕业证如何办理
原件一模一样【微信：95270640】【旧金山大学毕业证USF学位证成绩单】【微信：95270640】（留信学历认证永久存档查询）采用学校原版纸张、特殊工艺完全按照原版一比一制作（包括：隐形水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠，文字图案浮雕，激光镭射，紫外荧光，温感，复印防伪）行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备，十五年致力于帮助留学生解决难题，业务范围有加拿大、英国、澳洲、韩国、美国、新加坡，新西兰等学历材料，包您满意。
【业务选择办理准则】
一、工作未确定，回国需先给父母、亲戚朋友看下文凭的情况，办理一份就读学校的毕业证【微信：95270640】文凭即可
二、回国进私企、外企、自己做生意的情况，这些单位是不查询毕业证真伪的，而且国内没有渠道去查询国外文凭的真假，也不需要提供真实教育部认证。鉴于此，办理一份毕业证【微信：95270640】即可
三、进国企，银行，事业单位，考公务员等等，这些单位是必需要提供真实教育部认证的，办理教育部认证所需资料众多且烦琐，所有材料您都必须提供原件，我们凭借丰富的经验，快捷的绿色通道帮您快速整合材料，让您少走弯路。
留信网认证的作用:
1:该专业认证可证明留学生真实身份【微信：95270640】
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
→ 【关于价格问题（保证一手价格）
我们所定的价格是非常合理的，而且我们现在做得单子大多数都是代理和回头客户介绍的所以一般现在有新的单子 我给客户的都是第一手的代理价格，因为我想坦诚对待大家 不想跟大家在价格方面浪费时间
对于老客户或者被老客户介绍过来的朋友，我们都会适当给一些优惠。
选择实体注册公司办理，更放心，更安全！我们的承诺：可来公司面谈，可签订合同，会陪同客户一起到教育部认证窗口递交认证材料，客户在教育部官方认证查询网站查询到认证通过结果后付款，不成功不收费！
办理旧金山大学毕业证毕业证学位证USF学位证【微信：95270640 】外观非常精致，由特殊纸质材料制成，上面印有校徽、校名、毕业生姓名、专业等信息。
办理旧金山大学毕业证USF学位证毕业证学位证【微信：95270640 】格式相对统一，各专业都有相应的模板。通常包括以下部分：
校徽：象征着学校的荣誉和传承。
校名:学校英文全称
授予学位：本部分将注明获得的具体学位名称。
毕业生姓名：这是最重要的信息之一，标志着该证书是由特定人员获得的。
颁发日期：这是毕业正式生效的时间，也代表着毕业生学业的结束。
其他信息：根据不同的专业和学位，可能会有一些特定的信息或章节。
办理旧金山大学毕业证毕业证学位证USF学位证【微信：95270640 】价值很高，需要妥善保管。一般来说，应放置在安全、干燥、防潮的地方，避免长时间暴露在阳光下。如需使用，最好使用复印件而不是原件，以免丢失。
综上所述，办理旧金山大学毕业证毕业证学位证USF学位证【微信：95270640 】是证明身份和学历的高价值文件。外观简单庄重，格式统一，包括重要的个人信息和发布日期。对持有人来说，妥善保管是非常重要的。
Supermarket Management System Project Report.pdf
Supermarket management is a stand-alone J2EE using Eclipse Juno program.
This project contains all the necessary required information about maintaining
the supermarket billing system.
The core idea of this project to minimize the paper work and centralize the
data. Here all the communication is taken in secure manner. That is, in this
application the information will be stored in client itself. For further security the
data base is stored in the back-end oracle and so no intruders can access it.
Assistant Engineer (Chemical) Interview Questions.pdf
These are interview questions for the post of Assistant Engineer (Chemical)
Levelised Cost of Hydrogen (LCOH) Calculator Manual
The aim of this manual is to explain the
methodology behind the Levelized Cost of
Hydrogen (LCOH) calculator. Moreover, this
manual also demonstrates how the calculator
can be used for estimating the expenses associated with hydrogen production in Europe
using low-temperature electrolysis considering different sources of electricity
Call For Paper -3rd International Conference on Artificial Intelligence Advan...
* Registration is currently open *
Call for Research Papers!!!
Free – Extended Paper will be published as free of cost.
3rd International Conference on Artificial Intelligence Advances (AIAD 2024)
July 13 ~ 14, 2024, Virtual Conference
Webpage URL: https://aiad2024.org/index
Submission Deadline: June 22, 2024
Submission System URL:
https://aiad2024.org/submission/index.php
Contact Us:
Here's where you can reach us : aiad@aiad2024.org (or) aiadconference@yahoo.com
WikiCFP URL: http://wikicfp.com/cfp/servlet/event.showcfp?eventid=180509©ownerid=171656
#artificialintelligence #softcomputing #machinelearning #technology #datascience #python #deeplearning #tech #robotics #innovation #bigdata #coding #iot #computerscience #data #dataanalytics #engineering #robot #datascientist #software #automation #analytics #ml #pythonprogramming #programmer #digitaltransformation #developer #promptengineering #generativeai #genai #chatgpt #artificial #intelligence #datamining #networkscommunications #robotics #callforsubmission #submissionsopen #deadline #opencall #virtual #conference
This study Examines the Effectiveness of Talent Procurement through the Imple...
In the world with high technology and fast
forward mindset recruiters are walking/showing interest
towards E-Recruitment. Present most of the HRs of
many companies are choosing E-Recruitment as the best
choice for recruitment. E-Recruitment is being done
through many online platforms like Linkedin, Naukri,
Instagram , Facebook etc. Now with high technology E-
Recruitment has gone through next level by using
Artificial Intelligence too.
Key Words : Talent Management, Talent Acquisition , E-
Recruitment , Artificial Intelligence Introduction
Effectiveness of Talent Acquisition through E-
Recruitment in this topic we will discuss about 4important
and interlinked topics which are
Digital Twins Computer Networking Paper Presentation.pptx
A Digital Twin in computer networking is a virtual representation of a physical network, used to simulate, analyze, and optimize network performance and reliability. It leverages real-time data to enhance network management, predict issues, and improve decision-making processes.
Presentation on Food Delivery Systems
This presentation is about Food Delivery Systems and how they are developed using the Software Development Life Cycle (SDLC) and other methods. It explains the steps involved in creating a food delivery app, from planning and designing to testing and launching. The slide also covers different tools and technologies used to make these systems work efficiently.
Prediction of Electrical Energy Efficiency Using Information on Consumer's Ac...
Energy efficiency has been important since the latter part of the last century. The main object of this survey is to determine the energy efficiency knowledge among consumers. Two separate districts in Bangladesh are selected to conduct the survey on households and showrooms about the energy and seller also. The survey uses the data to find some regression equations from which it is easy to predict energy efficiency knowledge. The data is analyzed and calculated based on five important criteria. The initial target was to find some factors that help predict a person's energy efficiency knowledge. From the survey, it is found that the energy efficiency awareness among the people of our country is very low. Relationships between household energy use behaviors are estimated using a unique dataset of about 40 households and 20 showrooms in Bangladesh's Chapainawabganj and Bagerhat districts. Knowledge of energy consumption and energy efficiency technology options is found to be associated with household use of energy conservation practices. Household characteristics also influence household energy use behavior. Younger household cohorts are more likely to adopt energy-efficient technologies and energy conservation practices and place primary importance on energy saving for environmental reasons. Education also influences attitudes toward energy conservation in Bangladesh. Low-education households indicate they primarily save electricity for the environment while high-education households indicate they are motivated by environmental concerns.
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Call For Paper -3rd International Conference on Artificial Intelligence Advan...
This study Examines the Effectiveness of Talent Procurement through the Imple...
This study Examines the Effectiveness of Talent Procurement through the Imple...
Digital Twins Computer Networking Paper Presentation.pptx
Digital Twins Computer Networking Paper Presentation.pptx
Prediction of Electrical Energy Efficiency Using Information on Consumer's Ac...
Prediction of Electrical Energy Efficiency Using Information on Consumer's Ac...
1st summary rise.1st Summary-Genomics: Fish DNA Barcoding & Protein Profiling Workshop & My Life with Worms
- 1. 1st Summary-Genomics: Fish DNA Barcoding & Protein Profiling Workshop & My Life with Worms: Travelogue of Research with NemerteaJanuary 21-22, 2014 Dr. Pat Phelps & Dr. Jon. Norenburg DNA barcoding and protein profiling are techniques involving electrophoresis, in which molecules such as DNA and proteins on top of an electric field migrate down toward the oppositely charged electrode and separate according to their charge and shape. Electrophoresis reveals the number of proteins found in each sample as well as, their size and the similarities or differences between them. DNA barcoding is based on using a DNA strand to facilitate species identification by looking for patterns in sequences involved in replication of DNA, where DNA is extracted and purified. Then cytochrome c oxidase 1 gene is amplified by PCR, which results are observed through the agarose gel electrophoresis. In protein profiling, which identifies proteins and evolutionary relationships among fish, proteins are placed in microtubes containing a buffer. The samples are flicked, incubated and heated to denature the proteins before being loaded into the polyacrylamide gel solutions. After these techniques were presented, an expert in invertebrate studies discussed his research on analyzing the taxonomy and genetic patterns of Nemerteia to build a phylogenetic tree. Taxonomy, the classification of organisms, is one of the main goals of barcoding and protein profiling and it identifies the components of biological diversity, which provides basic knowledge of the principles of biological diversity.