Hershey and chase experiment-the blender experimentbiOlOgyBINGE
It was performed by Alfred Hershey and Martha Chase in 1952.
It led to confirmation of the genetic nature of DNA came from an experiment with E.coli phage T2.
for more details visit our youtube channel.
Hershey and chase experiment-the blender experimentbiOlOgyBINGE
It was performed by Alfred Hershey and Martha Chase in 1952.
It led to confirmation of the genetic nature of DNA came from an experiment with E.coli phage T2.
for more details visit our youtube channel.
Field-based, real-time metagenomics and phylogenomics for responsive pathogen...Joe Parker
Talk presented at the UK-India Joint Bioinformatics Workshop, Pirbright Institute, 09 Feb 2018
Abstract:
In a globalised world of increasing trade, novel threats to animal and plant health, as well as human diseases, can cross political and geographical borders spontaneously and rapidly. One such example is the rise of Acute Oak Decline (AOD) in the UK, a multifactorial decline syndrome with uncertain aetiology, vectors, and host risk factors first reported in the UK a decade ago. Affected oaks display significant morbidity and mortality, with symptoms including vascular interruption, crown loss and characteristic striking bark lesions breaching cambium and filled with a viscous, aromatic, dark-brown/black exudate, which may sometimes be released under considerable pressure. Although multiple bacterial species have been associated to lesion sites in affected oaks, and a putative insect vector identified, the basic risk factors, transmission, progression and treatment of the syndrome remain unclear.
This dispiriting state of affairs presents an ideal opportunity to exploit recent developments in nanopore sequencing to develop and test field-based methods of real-time phylogenomics and metagenomics to establish baseline data for healthy oaks, and contrast these with affected / dying oaks to shed light on syndrome causes and management. WGS metagenomic sampling was carried out on leaf and bark tissue from 37 affected, asymptomatic, and recovering individuals (nine Quercus species) at three field sites over a year. Extraction and DNA sequencing were performed in the field for a subset of samples with MinION nanopore rapid sequencing kits, and also using MinION and paired-end Illumina sequencing under laboratory conditions. Metagenomic analyses to determine microbial community composition were carried out, and real-time phylogenomic methods were also developed and applied. Early results from these analyses and lessons for future work are presented.
Metagenomic datasets can be rapidly generated in the field with minimal equipment using nanopore sequencing, providing a responsive capability for emerging disease threats and reducing transmission risks associated with transporting quantities of potentially infectious samples from outbreaks of novel diseases. Furthermore, real-time data analysis can provide rapid feedback to field teams, both to inform management decisions and also to allow for adaptive experimental protocols that dynamically target data collection to extract maximum information per unit effort.
This lecture covers key findings to the development of genomics as a field. This first part covers briefly Mendel to knowing that DNA is the genetic material by Hershey and Chase
Field-based, real-time metagenomics and phylogenomics for responsive pathogen...Joe Parker
Talk presented at the UK-India Joint Bioinformatics Workshop, Pirbright Institute, 09 Feb 2018
Abstract:
In a globalised world of increasing trade, novel threats to animal and plant health, as well as human diseases, can cross political and geographical borders spontaneously and rapidly. One such example is the rise of Acute Oak Decline (AOD) in the UK, a multifactorial decline syndrome with uncertain aetiology, vectors, and host risk factors first reported in the UK a decade ago. Affected oaks display significant morbidity and mortality, with symptoms including vascular interruption, crown loss and characteristic striking bark lesions breaching cambium and filled with a viscous, aromatic, dark-brown/black exudate, which may sometimes be released under considerable pressure. Although multiple bacterial species have been associated to lesion sites in affected oaks, and a putative insect vector identified, the basic risk factors, transmission, progression and treatment of the syndrome remain unclear.
This dispiriting state of affairs presents an ideal opportunity to exploit recent developments in nanopore sequencing to develop and test field-based methods of real-time phylogenomics and metagenomics to establish baseline data for healthy oaks, and contrast these with affected / dying oaks to shed light on syndrome causes and management. WGS metagenomic sampling was carried out on leaf and bark tissue from 37 affected, asymptomatic, and recovering individuals (nine Quercus species) at three field sites over a year. Extraction and DNA sequencing were performed in the field for a subset of samples with MinION nanopore rapid sequencing kits, and also using MinION and paired-end Illumina sequencing under laboratory conditions. Metagenomic analyses to determine microbial community composition were carried out, and real-time phylogenomic methods were also developed and applied. Early results from these analyses and lessons for future work are presented.
Metagenomic datasets can be rapidly generated in the field with minimal equipment using nanopore sequencing, providing a responsive capability for emerging disease threats and reducing transmission risks associated with transporting quantities of potentially infectious samples from outbreaks of novel diseases. Furthermore, real-time data analysis can provide rapid feedback to field teams, both to inform management decisions and also to allow for adaptive experimental protocols that dynamically target data collection to extract maximum information per unit effort.
This lecture covers key findings to the development of genomics as a field. This first part covers briefly Mendel to knowing that DNA is the genetic material by Hershey and Chase
1. University of Puerto Rico<br />Cayey Campus<br />Jessica Díaz Rivera<br />Prof. Elena Gonzalez<br />RISE Program<br />Gene to Protein Workshop<br /> In this workshop given by Patricia Casbas, Rodrigo Gonzalez, Sean Bailey and Daniel Dominguez we started discussing some information about the DNA. In the first experiment each student isolate their own DNA using cells from their mouth. The DNA that each student obtained had different sizes because of the variation in the source of DNA, the quantity that the person used to see their own DNA, or maybe the force that the person used to swirl clear Gatorade in the mouth. In the second experiment based on Separation and Detection we did a PCR (Polymerase Chain Reaction) to allows specific fragments of DNA to be copied and amplified. Later we did an electrophoresis to study a single gene out of over 21,000 using a specific protein called Green Fluorescence Protein (GFP). The third experiment was about Proteins and Methods for Detection, we used SDS-PAGE Electrophoresis to separate different sized proteins in polyacrylamide gel. With SDS-PAGE stained with coomassie we only separated proteins on a polyacrylamide gel, we did not did the antibody to detect a specific protein of interest. When we separated the proteins in the electrophoresis we can study the weight. The last workshop was about Macrophage infection with green fluorescent protein bacteria to see how macrophages eat foreign particles, including pathogens, in this case E. coli that was infected by GFP; also in the stereomicroscope we observed the cells of the macrophages and the macrophages with the E. coli; also we used UV light and Lux to saw the E. coli. To conclude all the workshop was about how DNA, genes, bacterias and proteins function. <br />