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ENZYMES IN
ANIMAL FEED
Patel Mili Nileshbhai
Msc.Microbiology
Semester : 2
201804101010020
ENZYMES
• Catalyze chemical processes
• Binding to specific substrates in the animal feed and
breaking them down into smaller components
• Highly efficient in its activity on specific substrates,
requiring only a small dosage to produce the desired
effect
• Exogenous enzymes created for use as animal feed
additives are produced by microbial fermentation of
substances found in plants, animals or
microorganisms
2
ANIMAL FEEDS
• Animal feed industry produce >750 million
tones of feed. >90% taken by poultry, pigs and
ruminants .10% taken by pet foods and fish
farming
• Many feed ingredients are not fully digested by
live stock so certain enzymes are adding to feed
• Enzymes are added to feed either directly or as
premix together with vitamins, minerals and
other feed additives
3
ENZYMES USED IN FEED
•Hydrolases
•Cellulase and hemicellulase
• 𝜷-glucanase and xylanases
4
HYDROLASES
• Main class of enzyme used in
monogastric feed
• Eliminate the anti-nutritional factor in
grains or vegetables
• Degrade certain cereal components
to improve the nutritional value of
feed
• Supplement the animals with their
own digestive enzymes, (e.g.
proteases, amylases, 𝛽-
glucanases.)
5
𝛽-GLUCANASES AND XYLANASES
• Hydrolyze non-starchy polysaccharides, like barley
𝛽-glucans and arabinoxylans
• Improve digestion and absorption of feed
components
• Improve weight gain by broiler chickens and egg
laying hens
• improve the performance of barley fed pigs
• Cannulated pig demonstrated, improved digestion
of starch, lipids, and proteins in small intestine
• Xylanase based multienzyme product reduce
overall cost of pig feed
6
CELLULASE AND HEMICELLULASE
• Improve feed utilization, milk yield
and weight gain by ruminants
• Improve nutritive quality of forages
• Success depends on stability in feed
and in rumen, capability to hydrolyze
plant cell wall polysaccharides,
capacity of animal to use reaction
product efficiently
• Cellulase and Xylanase to hay diet
increased weight gain of cattle much
as 35%
• 5-25% increase in milk yield reported
in dairy cows fed with forage treated
with commercial fibrolytic enzymes
7
COMMERCIAL PRODUCTION OF ENZYMES
• Identification and selection of a naturally occurring substance
that can be efficiently expressed by a microbial process and
maintain the required enzyme profile and activity
• Multiple animal feeding trials are conducted to confirm the
enzyme’s performance in animals
• Selected strain is propagated in liquid broth to produce a
large quantity of inoculum
• Adjust the temperature, nutrients, and air supply to ensure
optimal development
• Mixture of enzymes, nutrients, and microbes is strained
though a series of filters to remove impurities and extract the
enzymes
8
references:
• S.Shanmugam and T.Sathishkumar (2009) Enzyme Technology:
New Delhi: krishan makhijani.
• https://www.google.com/url?sa=t&source=web&rct=j&url=https://bri
worldwide.com/wp-
content/uploads/2014/11/BRI_EnzymeOverview_FINAL.pdf&ved=2a
hUKEwj0-
LLNrubhAhXTfn0KHYLqDCYQFjACegQIBBAR&usg=AOvVaw1a6N
k8JLf9BmMgzaJJTFRI
9
Thank you
10

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Enzymes in animal feed

  • 1. ENZYMES IN ANIMAL FEED Patel Mili Nileshbhai Msc.Microbiology Semester : 2 201804101010020
  • 2. ENZYMES • Catalyze chemical processes • Binding to specific substrates in the animal feed and breaking them down into smaller components • Highly efficient in its activity on specific substrates, requiring only a small dosage to produce the desired effect • Exogenous enzymes created for use as animal feed additives are produced by microbial fermentation of substances found in plants, animals or microorganisms 2
  • 3. ANIMAL FEEDS • Animal feed industry produce >750 million tones of feed. >90% taken by poultry, pigs and ruminants .10% taken by pet foods and fish farming • Many feed ingredients are not fully digested by live stock so certain enzymes are adding to feed • Enzymes are added to feed either directly or as premix together with vitamins, minerals and other feed additives 3
  • 4. ENZYMES USED IN FEED •Hydrolases •Cellulase and hemicellulase • 𝜷-glucanase and xylanases 4
  • 5. HYDROLASES • Main class of enzyme used in monogastric feed • Eliminate the anti-nutritional factor in grains or vegetables • Degrade certain cereal components to improve the nutritional value of feed • Supplement the animals with their own digestive enzymes, (e.g. proteases, amylases, 𝛽- glucanases.) 5
  • 6. 𝛽-GLUCANASES AND XYLANASES • Hydrolyze non-starchy polysaccharides, like barley 𝛽-glucans and arabinoxylans • Improve digestion and absorption of feed components • Improve weight gain by broiler chickens and egg laying hens • improve the performance of barley fed pigs • Cannulated pig demonstrated, improved digestion of starch, lipids, and proteins in small intestine • Xylanase based multienzyme product reduce overall cost of pig feed 6
  • 7. CELLULASE AND HEMICELLULASE • Improve feed utilization, milk yield and weight gain by ruminants • Improve nutritive quality of forages • Success depends on stability in feed and in rumen, capability to hydrolyze plant cell wall polysaccharides, capacity of animal to use reaction product efficiently • Cellulase and Xylanase to hay diet increased weight gain of cattle much as 35% • 5-25% increase in milk yield reported in dairy cows fed with forage treated with commercial fibrolytic enzymes 7
  • 8. COMMERCIAL PRODUCTION OF ENZYMES • Identification and selection of a naturally occurring substance that can be efficiently expressed by a microbial process and maintain the required enzyme profile and activity • Multiple animal feeding trials are conducted to confirm the enzyme’s performance in animals • Selected strain is propagated in liquid broth to produce a large quantity of inoculum • Adjust the temperature, nutrients, and air supply to ensure optimal development • Mixture of enzymes, nutrients, and microbes is strained though a series of filters to remove impurities and extract the enzymes 8
  • 9. references: • S.Shanmugam and T.Sathishkumar (2009) Enzyme Technology: New Delhi: krishan makhijani. • https://www.google.com/url?sa=t&source=web&rct=j&url=https://bri worldwide.com/wp- content/uploads/2014/11/BRI_EnzymeOverview_FINAL.pdf&ved=2a hUKEwj0- LLNrubhAhXTfn0KHYLqDCYQFjACegQIBBAR&usg=AOvVaw1a6N k8JLf9BmMgzaJJTFRI 9