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International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395 -0056
Volume: 04 Issue: 01 | Jan -2017 www.irjet.net p-ISSN: 2395-0072
© 2017, IRJET | Impact Factor value: 5.181 | ISO 9001:2008 Certified Journal | Page 615
STUDY ON BC SOIL USED AS SUBGRADE AND TREATED WITH
TERRAZYME- A BIO-ENZYME
A. Venkatesh1 and Dr. G. Sreenivasa Reddy2
1Post Graduate student of Civil Engineering, KSRM College (autonomous), Kadapa.
2Professor, Department of Civil Engineering, KSRM College (autonomous), Kadapa.
E-mail: 1andhevenkatesh1@gmail. com,2transgsr@gmail.com
---------------------------------------------------------------------***---------------------------------------------------------------------
Abstract - In India vast area was occupied by Black Cotton
Soil which contains clay as chief constituent. These soils show
minimal bearing capacity and low strength. They swell when
come in contact with water and shrink as they dry out.
Engineers face problems with such type of soils, which do not
possess sufficient strength to carry the imposed loads during
construction and the life of the structure. Properties of the soil
must be increased to achieve economy and to improve the
performance of structures.
Soil stabilization is the process of reducing
undesirable behaviour of expansive soils. Conventional
stabilization methods are time consuming and are proved to
be not economical; it became necessary to look for alternative
eco-friendly stabilizers like bio-enzymes. Recently there are
many bio-enzymes available for soil stabilization such as
Renolith, Permazyme, Terrazyme and Fujibeton, proved to be
effective and economical.
The present study deals the effect of Terrazymeabio-
enzyme on shear strength and CBR value of expansive soil.
Laboratory testswereconductedonvarious dosages, stabilizer
was added by replacing 1%,2%,3%,4% of the optimum water
content of the black cotton soil.
Key Words: Expansive soil, Terrazyme, Shear strength,
CBR.
1.INTRODUCTION: Infrastructure projects such as
highways, railways, etc. requires soil material in very large
quantity. Usually, large areas are covered with highly plastic
and expansive soil, which is not suitable for such purpose.
Extensive laboratory tests have been carried out by various
researchers using saw dust ash and have shown promising
results for application of such expansive soil after
stabilization.
1.1. Objectives of Present Study
1. To study the properties of the black cotton
soil.
2. To study the effect of Terrazyme on shear
strength and CBR value of black cottonsoil.
2. LITERATURE REVIEW
BERGMANN (2000) through his experimental
studies, he conclude that Bio-Enzymes need some clay
percentage to strengthen the soils. He found that
minimum of 2% clay must be present for successful
stabilization, 10 to 15% of clay will show better results.
SHARMA (2006) has conducted experimental
studies Bio-Enzymatic stabilizationofthreevarietytype
of soils namely high plastic clay (CH), low plastic clay
(CL), and low plastic silt (ML). His results showed that
CH soil showed improvement in CBR value with
saturation moisture reduction from 40 to 21% after 4
weeks of stabilization. Also it was found that there was
100% improvement in UCS.
MITHANTHAYA.I.R, HARSHA KUMAR RAI and
RAVISHANKAR A.U (2009) investigated the modified
geo-technical properties of the lateritic soils by
stabilizing withenzyme.QuantitychangesinCBRvalues,
UCC and Permeability were observed withfourdifferent
dosages of Terrazyme. 200ml of Terrazyme was added
to 2, 2.5,3 and 3.5m3 of soil. CBR value was increased by
400%, UCC value was increased by 450% and
Permeability was reduced by 42%.
3.MATERIALS AND METHODOLOGY:
In this process the soil sample is collected from
RAMARAJUPALLIvillagenearKadapa.The experimental
investigations on soil sample aredonetounderstandthe
index and engineering properties of the collected soil
sample. This experimental study helps to classify the
soil. The obtained values of index and engineering
properties of the soil.
International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395 -0056
Volume: 04 Issue: 01 | Jan -2017 www.irjet.net p-ISSN: 2395-0072
© 2017, IRJET | Impact Factor value: 5.181 | ISO 9001:2008 Certified Journal | Page 616
Figure 3.1. Black Cotton Soil Sample
Terrazyme is obtained from Avijeet Agencies
Chennai.
Figure 3.2. Black Cotton Soil Sample
4 BLACK COTTON SOIL PROPERTIES
Table 1: Properties of Black Cotton Soil Sample
S.No Experiments Results
1. Specific Gravity of
Soil Particles
2.34
2. Liquid Limit 66%
3. Plastic Limit 28%
4. Plasticity Index 38%
5. Grain Size
Analysis
Clay %
Silt %
Sand %
68%
18%
14%
6. IS Classification
of Soil
CH
7. Free Swell Index 85%
8. Standard Proctor OMC-26%
Compaction Test MDD-1.36 gm/cc
9. California
Bearing Ratio
Un-soaked- 3.93
Soaked-2.48
10. Unconfined
Compression Test
Un-
soaked
Soaked
for 4
days
Soaked
for 7
days
Shear Strength
(kPa)
5.39 10.8 17.65
5. PROPERTIES OF TERRAZYME
Table 2: Properties of Terrazyme
Colour Dark Brown
PH 3.50
Specific Gravity 1.414
Evaporation Rate Same as Water
Odour Smell like Molasses
Extracted from Molasses
Source: Stabilization of Black-Cotton Soil using Bio-
Enzyme for a Highway Material by Joydeep Sen and
Jitendra Prasad Singh.
6. RESULTS AND DISCUSSIONS
Unconfined Compression Test Result:
Unconfined Compressionisperformedtodetermine
the shear strength of the soil. Unconfined Compression
samples are placed kept for a curing period of 4 and 7
days.
Table 3: Shear Strength obtained from
Unconfined Compression test of soil treated
with Terrazyme at various percentages forboth
cured and un-cured specimens.
DOSAGE OF
TERRAZYME
SHEAR STRENGTH(kPa)
Samples
of 0 days
of curing
Samples
of 4 days
of curing
Samples
of 7 days
of curing
International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395 -0056
Volume: 04 Issue: 01 | Jan -2017 www.irjet.net p-ISSN: 2395-0072
© 2017, IRJET | Impact Factor value: 5.181 | ISO 9001:2008 Certified Journal | Page 617
0%
Terrazyme
5.39 10.8 17.65
1%
Terrazyme
11.77 29.43 40.71
2%
Terrazyme
21.58 35.80 58.36
3%
Terrazyme
25.50 41.20 72.55
4%
Terrazyme
27.50 49.05 78.48
Figure 6.1. Shear Strength variation with
percentage of Terrazyme and days of curing.
CBR Test Result:
CBR test is performed to determine the CBR value.
To ascertain the behaviour of soil under soaked conditions
the samples are soaked for 4 days.
Table 4: CBR values with varying percentages
of Terrazyme for both soaked and un-soaked
soil samples.
DOSAGE OF
TERRAZYME
CBR Value
Un-Soaked Soaked for 4
days
0% Terrazyme 3.93 2.48
1% Terrazyme 5.25 4.43
2% Terrazyme 6.23 4.59
3% Terrazyme 7.38 4.92
4% Terrazyme 8.03 6.39
Figure 6.2. Variation of CBR values with
percentage of Terrazyme and days of soaking.
International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395 -0056
Volume: 04 Issue: 01 | Jan -2017 www.irjet.net p-ISSN: 2395-0072
© 2017, IRJET | Impact Factor value: 5.181 | ISO 9001:2008 Certified Journal | Page 618
Tri-Axial Test Result:
Tri-Axial test is performed to determine the shear
parameters of the soil.
Table 5: Variation of Shear Parameters and
Shear Strength with variation in percentage of
Terrazyme.
Figure 6.3. Variation of cohesion with percentage
variation of Terrazyme
7. COCLUSIONS:
1. Shear Strength of the soil increased form
5.39 kPa at 0% Terrazyme to 27.5 kPa at 4%
Terrazyme, percentage increase is 410%.
2. With increase in percentage of Terrazyme
and days of curing shear strength of the soil
increased from 10.8 kPa to 49.05 kPa, results are
tabulated for 4 days of curing period. Percentage
increase is 354.1%.
3. With increase in percentage of Terrazyme
and days of curing shear strength of the soil
increased from 17.65 kPa to 78.48 kPa, results
are tabulated for 7 days of curing period.
Percentage increase is 344.6%.
4. With increase in percentage of Terrazyme the un-
soaked CBR value was increased from 3.93 to
8.03. Percentage increase is 104.32.
5. With increase in soaking period CBR
values are increased when compared with
untreated soaked soil samples.
6. Tri-Axial results showed, with increase in
percentage of Terrazyme shear strength of the
soil increased from 6.40 kPa to 35.32 kPa,
percentage increase is 451.87.
7. Tri-Axial results showed with increase in
percentage of Terrazyme, cohesion of the soil
increased from 6.85 kPa at 0% Terrazyme to
38.60 kPa at 4% Terrazyme, percentage increase
is 463.50.
REFERENCES
1. Venika Saini and Priyanka Vaishnava,"SOIL
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2. Lekha.B.M, Goutham.S, Chaitali.N, and Ravi
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3. Manjunath.C, Pavaitra.D and Amarnath.M.S," A
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EFFECT OF BIO-ENZYMATIC
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0%
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Angle of
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International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395 -0056
Volume: 04 Issue: 01 | Jan -2017 www.irjet.net p-ISSN: 2395-0072
© 2017, IRJET | Impact Factor value: 5.181 | ISO 9001:2008 Certified Journal | Page 619
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GEOTECHNICAL CONFERENCE, 17th – 19th
DECEMBER 2015, Pune, Maharashtra, India.
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11, Number 10 (2016) pp 6940-6942.
14. Ishwarya S Dhanesh and Twinkle Vinu
Mohandas, "Effect of bio-enzyme on
geotechnical propertiesofThonnakkal clay",Int
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June 2016.
15. Vijay Rajoria and Suneet Kaur "A REVIEW ON
STABILIZATION OF SOIL USING BIO-ENZYME",
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16. Puneet Agarwal and Suneet Kaur "EFFECT OF
BIO-ENZYME STABILIZATION ON
UNCONFINED COMPRESSIVE STRENGTH OF
EXPANSIVE SOIL",International Journal of
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eISSN: 2319-1163 | pISSN: 2321-7308.
17. Priyanka M Shaka and Surekha M Shaka"
LABORATORY INVESTIGATION ON BLACK
COTTON SOILS AND RED SOIL STABILIZED
USING ENZYME", IRJET, Volume: 03 Issue: 06 |
June-2016.
18. Sudeepa Shetty, Dhanraj V, 3Ashwini Pai, Jeenu
P Gopi, Prashantha,"QUALITATIVE STUDY ON
PHYSICAL AND BIO-ENZYMATIC
STABILISATION OF LATERITIC SOIL", August
2016, Volume 3, Issue 8 JETIR (ISSN-2349-
5162), JETIR1608004.
19. Purnima Bajpai," NON-CONVENTIONAL SOIL
STABILIZATION TECHNIQUES THE WAY
FORWARD TO AN AGGREGATE FREE
PAVEMENT AND A COST EFFECTIVE METHOD
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5518.
20. Guruprasad Jadhav, Ganesh Panchal and
Randhir Mane, " A STUDY ON EXPERIMENTAL
INVESTIGATION OF BIO-ENZYME STABILIZED
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on Recent Trends in Engineering Science and
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21. Uzaira Rafique, Saima Nasreen , Rabbiya
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22. Sravan.M.V and Nagaraj.H.B, "PRELIMINARY
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First International Conference on Bio-based
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Ferrand, France.

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Study on BC Soil Used as Subgrade and Treated with Terrazyme- A Bio-Enzyme

  • 1. International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395 -0056 Volume: 04 Issue: 01 | Jan -2017 www.irjet.net p-ISSN: 2395-0072 © 2017, IRJET | Impact Factor value: 5.181 | ISO 9001:2008 Certified Journal | Page 615 STUDY ON BC SOIL USED AS SUBGRADE AND TREATED WITH TERRAZYME- A BIO-ENZYME A. Venkatesh1 and Dr. G. Sreenivasa Reddy2 1Post Graduate student of Civil Engineering, KSRM College (autonomous), Kadapa. 2Professor, Department of Civil Engineering, KSRM College (autonomous), Kadapa. E-mail: 1andhevenkatesh1@gmail. com,2transgsr@gmail.com ---------------------------------------------------------------------***--------------------------------------------------------------------- Abstract - In India vast area was occupied by Black Cotton Soil which contains clay as chief constituent. These soils show minimal bearing capacity and low strength. They swell when come in contact with water and shrink as they dry out. Engineers face problems with such type of soils, which do not possess sufficient strength to carry the imposed loads during construction and the life of the structure. Properties of the soil must be increased to achieve economy and to improve the performance of structures. Soil stabilization is the process of reducing undesirable behaviour of expansive soils. Conventional stabilization methods are time consuming and are proved to be not economical; it became necessary to look for alternative eco-friendly stabilizers like bio-enzymes. Recently there are many bio-enzymes available for soil stabilization such as Renolith, Permazyme, Terrazyme and Fujibeton, proved to be effective and economical. The present study deals the effect of Terrazymeabio- enzyme on shear strength and CBR value of expansive soil. Laboratory testswereconductedonvarious dosages, stabilizer was added by replacing 1%,2%,3%,4% of the optimum water content of the black cotton soil. Key Words: Expansive soil, Terrazyme, Shear strength, CBR. 1.INTRODUCTION: Infrastructure projects such as highways, railways, etc. requires soil material in very large quantity. Usually, large areas are covered with highly plastic and expansive soil, which is not suitable for such purpose. Extensive laboratory tests have been carried out by various researchers using saw dust ash and have shown promising results for application of such expansive soil after stabilization. 1.1. Objectives of Present Study 1. To study the properties of the black cotton soil. 2. To study the effect of Terrazyme on shear strength and CBR value of black cottonsoil. 2. LITERATURE REVIEW BERGMANN (2000) through his experimental studies, he conclude that Bio-Enzymes need some clay percentage to strengthen the soils. He found that minimum of 2% clay must be present for successful stabilization, 10 to 15% of clay will show better results. SHARMA (2006) has conducted experimental studies Bio-Enzymatic stabilizationofthreevarietytype of soils namely high plastic clay (CH), low plastic clay (CL), and low plastic silt (ML). His results showed that CH soil showed improvement in CBR value with saturation moisture reduction from 40 to 21% after 4 weeks of stabilization. Also it was found that there was 100% improvement in UCS. MITHANTHAYA.I.R, HARSHA KUMAR RAI and RAVISHANKAR A.U (2009) investigated the modified geo-technical properties of the lateritic soils by stabilizing withenzyme.QuantitychangesinCBRvalues, UCC and Permeability were observed withfourdifferent dosages of Terrazyme. 200ml of Terrazyme was added to 2, 2.5,3 and 3.5m3 of soil. CBR value was increased by 400%, UCC value was increased by 450% and Permeability was reduced by 42%. 3.MATERIALS AND METHODOLOGY: In this process the soil sample is collected from RAMARAJUPALLIvillagenearKadapa.The experimental investigations on soil sample aredonetounderstandthe index and engineering properties of the collected soil sample. This experimental study helps to classify the soil. The obtained values of index and engineering properties of the soil.
  • 2. International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395 -0056 Volume: 04 Issue: 01 | Jan -2017 www.irjet.net p-ISSN: 2395-0072 © 2017, IRJET | Impact Factor value: 5.181 | ISO 9001:2008 Certified Journal | Page 616 Figure 3.1. Black Cotton Soil Sample Terrazyme is obtained from Avijeet Agencies Chennai. Figure 3.2. Black Cotton Soil Sample 4 BLACK COTTON SOIL PROPERTIES Table 1: Properties of Black Cotton Soil Sample S.No Experiments Results 1. Specific Gravity of Soil Particles 2.34 2. Liquid Limit 66% 3. Plastic Limit 28% 4. Plasticity Index 38% 5. Grain Size Analysis Clay % Silt % Sand % 68% 18% 14% 6. IS Classification of Soil CH 7. Free Swell Index 85% 8. Standard Proctor OMC-26% Compaction Test MDD-1.36 gm/cc 9. California Bearing Ratio Un-soaked- 3.93 Soaked-2.48 10. Unconfined Compression Test Un- soaked Soaked for 4 days Soaked for 7 days Shear Strength (kPa) 5.39 10.8 17.65 5. PROPERTIES OF TERRAZYME Table 2: Properties of Terrazyme Colour Dark Brown PH 3.50 Specific Gravity 1.414 Evaporation Rate Same as Water Odour Smell like Molasses Extracted from Molasses Source: Stabilization of Black-Cotton Soil using Bio- Enzyme for a Highway Material by Joydeep Sen and Jitendra Prasad Singh. 6. RESULTS AND DISCUSSIONS Unconfined Compression Test Result: Unconfined Compressionisperformedtodetermine the shear strength of the soil. Unconfined Compression samples are placed kept for a curing period of 4 and 7 days. Table 3: Shear Strength obtained from Unconfined Compression test of soil treated with Terrazyme at various percentages forboth cured and un-cured specimens. DOSAGE OF TERRAZYME SHEAR STRENGTH(kPa) Samples of 0 days of curing Samples of 4 days of curing Samples of 7 days of curing
  • 3. International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395 -0056 Volume: 04 Issue: 01 | Jan -2017 www.irjet.net p-ISSN: 2395-0072 © 2017, IRJET | Impact Factor value: 5.181 | ISO 9001:2008 Certified Journal | Page 617 0% Terrazyme 5.39 10.8 17.65 1% Terrazyme 11.77 29.43 40.71 2% Terrazyme 21.58 35.80 58.36 3% Terrazyme 25.50 41.20 72.55 4% Terrazyme 27.50 49.05 78.48 Figure 6.1. Shear Strength variation with percentage of Terrazyme and days of curing. CBR Test Result: CBR test is performed to determine the CBR value. To ascertain the behaviour of soil under soaked conditions the samples are soaked for 4 days. Table 4: CBR values with varying percentages of Terrazyme for both soaked and un-soaked soil samples. DOSAGE OF TERRAZYME CBR Value Un-Soaked Soaked for 4 days 0% Terrazyme 3.93 2.48 1% Terrazyme 5.25 4.43 2% Terrazyme 6.23 4.59 3% Terrazyme 7.38 4.92 4% Terrazyme 8.03 6.39 Figure 6.2. Variation of CBR values with percentage of Terrazyme and days of soaking.
  • 4. International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395 -0056 Volume: 04 Issue: 01 | Jan -2017 www.irjet.net p-ISSN: 2395-0072 © 2017, IRJET | Impact Factor value: 5.181 | ISO 9001:2008 Certified Journal | Page 618 Tri-Axial Test Result: Tri-Axial test is performed to determine the shear parameters of the soil. Table 5: Variation of Shear Parameters and Shear Strength with variation in percentage of Terrazyme. Figure 6.3. Variation of cohesion with percentage variation of Terrazyme 7. COCLUSIONS: 1. Shear Strength of the soil increased form 5.39 kPa at 0% Terrazyme to 27.5 kPa at 4% Terrazyme, percentage increase is 410%. 2. With increase in percentage of Terrazyme and days of curing shear strength of the soil increased from 10.8 kPa to 49.05 kPa, results are tabulated for 4 days of curing period. Percentage increase is 354.1%. 3. With increase in percentage of Terrazyme and days of curing shear strength of the soil increased from 17.65 kPa to 78.48 kPa, results are tabulated for 7 days of curing period. Percentage increase is 344.6%. 4. With increase in percentage of Terrazyme the un- soaked CBR value was increased from 3.93 to 8.03. Percentage increase is 104.32. 5. With increase in soaking period CBR values are increased when compared with untreated soaked soil samples. 6. Tri-Axial results showed, with increase in percentage of Terrazyme shear strength of the soil increased from 6.40 kPa to 35.32 kPa, percentage increase is 451.87. 7. Tri-Axial results showed with increase in percentage of Terrazyme, cohesion of the soil increased from 6.85 kPa at 0% Terrazyme to 38.60 kPa at 4% Terrazyme, percentage increase is 463.50. REFERENCES 1. Venika Saini and Priyanka Vaishnava,"SOIL STABILIZTION BY USING TERRAZYME", Vol. 8, International Journal of Advances in Engineering & Technology,Issue4,pp.566-573 Aug., 2015. 2. Lekha.B.M, Goutham.S, Chaitali.N, and Ravi Shankar.A.U, " LABORATORY INVESTIGATION ON BLACK COTTON SOIL STABILIZED WITH NON TRADITIONALSTABILIZER"e-ISSN:2278- 1684, p-ISSN: 2320-334X, PP 07-13. 3. Manjunath.C, Pavaitra.D and Amarnath.M.S," A STUDY ON EFFECT OF DOSAGE OF BIO- ENZYME ON STRENGTH AND PLASTICITY CHARACTERISTICS OF SOILS." 4. Chaudhary.S.K.,"INNOVATIVECONSTRUCTION TECHNOLOGY FOR QUALITY CONSTRUCTION OF RURAL ROAD", Road Engineering Rural Infrastructure, August-2012. 5. Joydeep Sen and Jitendra Prasad Singh, "STABILIZATION OF BLACK COTTON SOIL USING BIO-ENZYME FOR A HIGHWAY MATERIAL",IJIRSET, Volume-4, Issue-12, December-2015. 6. Venkatasubramanian.C and Dhinakaran.G, " EFFECT OF BIO-ENZYMATIC SOILNSTABILIZATION ON UNCONFINED SHEAR PARAMET ERS DOSAGE OF TERRAZYME BC Soil + 0% Terrazy me BC Soil + 1% Terraz yme BC Soil + 2% Terrazy me BC Soil + 3% Terraz yme BC Soil + 4% Terraz yme Cohesion (kPa) 6.40 9.80 17.70 24.50 35.32 Angle of Internal Friction 2◦ 50◦ 2◦ 10◦ 1◦ 50◦ 1◦ 10◦ 0◦ 40◦ Shear Strength (kPa) 6.85 18.20 25.20 29.70 38.60
  • 5. International Research Journal of Engineering and Technology (IRJET) e-ISSN: 2395 -0056 Volume: 04 Issue: 01 | Jan -2017 www.irjet.net p-ISSN: 2395-0072 © 2017, IRJET | Impact Factor value: 5.181 | ISO 9001:2008 Certified Journal | Page 619 COMPRESSIVE STRENGTH AND CALIFORNIA BEARING RATIO" Journal of Engineering and Applied Sciences, ISSN:1816-949X,295-298. 7. Tanveer Ahmed Khan and Mohd Raihan Taha, "EFFECT OF THREE BIOENZYMES ON COMPACTION, CONSISTENCY LIMITS, AND STRENGTH CHARACTERISTICS OF A SEDIMENTARY RESIDUAL SOIL", Advances in Material Sciences and Engineering, Volume- 2015. 8. Nandini.D.N, Vinoda.A and Prathap Kumar.M.T, "COMPACTION AND STRENGTH CHARACTERISTICS OF TERRA-ZYME STABILIZED RED SOIL" ,IJRPETM, Volume- 1,Issue-1, July-2015. 9. Srinivasa.G and Amith Kadaba Sheshadri, "BLACK COTTON SOIL STABILIZATION BY BIO ENZYMES ", AIJREAS, Volume -1, Issue 10 (2016, oct). 10. Sureka NaageshandGangadhara.S," SWELLING PROPERTIES OF BIO-ENZYME TREATED EXPANSIVE SOIL" International Journal of Engineering Studies”ISSN 0975- 6469 Volume 2, Number 2 (2010), pp. 155–159. 11. Ravi Shankar.A.U, Harsha Kumar Rai and Ramesha Mithanthaya.I, " BIO-ENZYME STABILIZED LATERITIC SOIL AS A HIGHWAY MATERIAL" , Journal of the Indian Roads Congress, July-September 2009. 12. Ramesh.H.N andSagar.S.R,"EFFECTOF DRYING ON THE STRENGTH PROPERTIES OF TERRAZYME TREATED EXPANSIVE ANDNON- EXPANSIVE SOILS ",50th INDIAN GEOTECHNICAL CONFERENCE, 17th – 19th DECEMBER 2015, Pune, Maharashtra, India. 13. Ansu Thomas, Tripathi.R.K, and Yadu.L.K, "EFFECT OF ENZYMES ON THE UNCONFINED COMPRESSIVE STRENGTH OF SOIL",International Journal of Applied Engineering Research ISSN 0973-4562Volume 11, Number 10 (2016) pp 6940-6942. 14. Ishwarya S Dhanesh and Twinkle Vinu Mohandas, "Effect of bio-enzyme on geotechnical propertiesofThonnakkal clay",Int ernational Journal of Engineering Trends and Technology (IJETT) – Volume 36 Number 9 - June 2016. 15. Vijay Rajoria and Suneet Kaur "A REVIEW ON STABILIZATION OF SOIL USING BIO-ENZYME", International Journal of Research in EngineeringandTechnology,eISSN:2319-1163 | pISSN: 2321-7308. 16. Puneet Agarwal and Suneet Kaur "EFFECT OF BIO-ENZYME STABILIZATION ON UNCONFINED COMPRESSIVE STRENGTH OF EXPANSIVE SOIL",International Journal of Research in Engineering and Technology, eISSN: 2319-1163 | pISSN: 2321-7308. 17. Priyanka M Shaka and Surekha M Shaka" LABORATORY INVESTIGATION ON BLACK COTTON SOILS AND RED SOIL STABILIZED USING ENZYME", IRJET, Volume: 03 Issue: 06 | June-2016. 18. Sudeepa Shetty, Dhanraj V, 3Ashwini Pai, Jeenu P Gopi, Prashantha,"QUALITATIVE STUDY ON PHYSICAL AND BIO-ENZYMATIC STABILISATION OF LATERITIC SOIL", August 2016, Volume 3, Issue 8 JETIR (ISSN-2349- 5162), JETIR1608004. 19. Purnima Bajpai," NON-CONVENTIONAL SOIL STABILIZATION TECHNIQUES THE WAY FORWARD TO AN AGGREGATE FREE PAVEMENT AND A COST EFFECTIVE METHOD OF ROAD CONSTRUCTION",International Journal of Scientific & Engineering Research, Volume 5, Issue 6, June-2014 1063 ISSN 2229- 5518. 20. Guruprasad Jadhav, Ganesh Panchal and Randhir Mane, " A STUDY ON EXPERIMENTAL INVESTIGATION OF BIO-ENZYME STABILIZED EXPANSIVE SOIL ",5th International Conference on Recent Trends in Engineering Science and Management, 9-10 December-2016. 21. Uzaira Rafique, Saima Nasreen , Rabbiya Naveed and Muhammad Aqeel Ashraf," APPLICATION OF BIOENZYMATIC SOIL STABILIZATION IN COMPARISON TO MACADAM IN THE CONSTRUCTION OF TRANSPORT INFRASTRUCTURE", Journal of Environmental Biology, Vol. 37 (Special Issue), 1209-1215, September 2016. 22. Sravan.M.V and Nagaraj.H.B, "PRELIMINARY STUDY ON USE OF TERRAZYME AS A BIO- STABILIZER ALONG WITH CEMENT AND LIME IN COMPRESSEDSTABILIZEDEARTHBLOCKS", First International Conference on Bio-based Building Materials, June22-24 Clemont- Ferrand, France.