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International Journal of Trend in Scientific Research and Development (IJTSRD)
Volume 6 Issue 4, May-June 2022 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470
@ IJTSRD | Unique Paper ID – IJTSRD50109 | Volume – 6 | Issue – 4 | May-June 2022 Page 693
Analysis of Food Coloring
Aanchal Alok1
, Dr. H. L. Saxena2
1
Research Scholar,
1,2
Department of Chemistry, Himalayan University, Itanagar, Arunachal Pradesh, India
ABSTRACT
This study was conducted to bring to light food coloring used in three
selected dishes. The dishes were taken naming ice-cream, candy and
tomato sauce applying convenience sampling method. The study
revealed to take part 78.4% non food grade and 21.5 % food grade
colors in widespread use in these three dishes. Various textile dye
and cosmetic color were in abundance used as non food grade colors
and various synthetic colors e. g. amaranth red and ponceau 4R were
as food grade colors in the selected dishes.
KEYWORDS: Food Coloring, Dishes, Food Grade Colors, Non Food
Grade Colors
How to cite this paper: Aanchal Alok |
Dr. H. L. Saxena "Analysis of Food
Coloring" Published
in International
Journal of Trend in
Scientific Research
and Development
(ijtsrd), ISSN: 2456-
6470, Volume-6 |
Issue-4, June 2022,
pp.693-695, URL:
www.ijtsrd.com/papers/ijtsrd50109.pdf
Copyright © 2022 by author(s) and
International Journal of Trend in
Scientific Research and Development
Journal. This is an
Open Access article
distributed under the
terms of the Creative Commons
Attribution License (CC BY 4.0)
(http://creativecommons.org/licenses/by/4.0)
1. General Background
Food coloring is the dye, pigment or substances
adhered to foods or drinks in order to alter the color
or the trick to sustain the prevailing colors with
attractive flavors to gain affluent aesthetic look. Food
coloring is available in liquids, gels, powders and
pastes forms to be used both in commercial food
production and in domestic cooking. Food coloring is
used in a variety of non food applications including
cosmetics, home crafts, pharmaceuticals and medical
devices by din of their safety and general
availability.[1,2]
The color additives main purpose is to seem more
inviting and appealing, especially to the children. The
color in foods losses due to exposure to light, air,
temperature, moisture and storage conditions. It
corrects natural color variations and enhances natural
existing colors. It provides color to colorless and
"fun" foods. Food color has always been a value of
quality. Without color additives, margarine won't be
yellow, colas won't be brown and mint ice-cream
won't be green. Color additives have come into
fashion as vital part of all foods we eat.
History
The addition of colorant to foods is thought to have
happened in Egyptian cities, where candy makers
around 1500 BC added natural extracts and wine to
improve product appearance. Up to the middle of the
19th century ingredients such as the spice saffron
from the local areas to the production units were
added for decorative effect to certain foodstuffs.
In 1856, the first synthetic color (mauvine) was
developed by Sir William Henry Perkin and by the
turn of the century, unmonitored color additives had
spread through the USA and Europe in all the source
of popular foods, including mustard, jellies, wine and
ketchup. Sellers at the time offered more than 80
artificial coloring agents, some intended for dyeing
textiles, not foods. As the 1900s starting, the bulk of
chemically synthesized colors were derived from
aniline, a petroleum product in toxic nature.
Chemically synthesized colors were easier to
produce, less expensive and superior in coloring
properties.[3,4]
Type of clolors
The food permitted colors are some naturally derived
colors, principally plant extracts, their chemically
identical synthetic duplicates and chemically
IJTSRD50109
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD50109 | Volume – 6 | Issue – 4 | May-June 2022 Page 694
synthesized compounds. The coloring matters gone to
foods may be grouped into main 2 classes; natural
and synthetic or artificial colors by name.
Natural colors are approved for foods include
carmines or cochineal (red), carotene (orange red),
saffron (orange), anthocyanin (blue), curcumin
(orange and yellow),chlorophyll (green), annatto
(orange) and caramel (dark brown) etc.
Synthetic or artificial colors (some are adopted
worldwide) include,
Red colorants: Amaranth, carmoisine, erythrosine,
fast red E, ponceau 4R and allura red AC.
1. Orange or yellow colorants: Tartazine, yellow 2G,
sunset yellow FCF.
2. Blue colorants: Brilliant blue FCF, Indigo
carmine.
3. Green colorants: Green FCF, Fast green S .[5,6]
Health Impact
The approved colors aren't always safe. Moreover,
even the safest permitted color, if taken in excessive
range proved detrimental for human health. Since
synthetic dyes contained various heavy metals like
Pb, Hg, As, Cu, Ni, Mg, Co etc., various studies have
found that it can cause hyperactivity and lapses of
concentration in children. Coloring also cause triggers
hyperactivity in some sensitive children, reduce liver
and duct activity, rises thyroid tumors, increase
allergy-like reactions primarily in aspirin- sensitive
fellows, causes tumors of adrenal gland and kidney,
carcinogenic and finally death. Eating foods
containing industrial dyes and colors causes violent
allergic reactions, respiratory problems, asthma, liver
disorders and kidney dysfunction and bone marrow
disorders.
Food, as a basic need for all people must become
wholesome and safe. It's only nutritive when it is
pure, fresh and free from hazardous matters. Food
coloring is a premier public hazard affecting the
quality of life. The nature of food coloring varies
from place to place. Due to illiteracy and poor
knowledge on food colors, many industries use
banned and synthetic or artificial colors to produce a
lot of food in the country. Considering the various
toxic effects of colors, the current study was planned
to survey on various food color materials, including
the banned ones (textile or cosmetic colors), natural,
artificial in terms of development, regulations and
technical regulations.[7,8]
Framework
Study Nature
The study was an observational study.
Sample Size and Information Collection
There were framed a total of 40 samples from 20 food
industries to carry on the study. Industries' owners,
labors and food vendors helped to collect the
information in this connection.
Questionnaire Development
A standard questionnaire was developed to gain the
relevant information on the study. The query were
designed, pretested, modified and resettled to achieve
the recorded information easily.
Data Aggregation
Data was aggregated by asking question to the factory
owners, labors, respective produces venders and color
sellers in the market.[9,10]
Data Verification
Questionnaire were checked each day after
interviewing and again after completion of data
aggregation and coded before entering into computer
technology. The data was edited if there was any
discrepancy (doubt entry, wrong entry etc.).
Statistical Analysis
All the statistical analysis and all other data
processing were done by using specialized analytical
software program. Microsoft Word and Microsoft
Excel were in frequent application on tabular, chart
and graphical representation. All the statistical
analysis were done very carefully in order to obtain
the good accuracy of result.
Results
The study on colors using in selected 40 samples of
three produces was conducted.
Table 1 represented the different colors used in total
20 industries' 40 food samples off selected three
dishes.
According to the value of table 1, it was showed in
items of ice-cream that 42% red, 35% green and 23%
yellow, the percentage of panceau 4R and red color
was in candy making was 77% and 23%. In Tomato
sauce's amaranth red color.
The food grade colors were found only in 10 and the
rest 30 items were made from non food grade colors.
The figure 1 represented the percentage of non food
grade and food grade colors used in producing ice-
cream, candy and tomato sauce was found as 78.6%
and 21.5% respectively.
Discussion
From the results, it is observed that yellow, red and
green colors were used for e production of ice-cream
which is recognized as textile dye otherwise cosmetic
olors due to the ignorance and illiteracy of e
manufacturers making the ultimate ictimization to the
consumers. This scene as not only common in case of
ice cream ut also hawaii methai and particularly andy.
In candy production procedure, onceau 4R partially
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD50109 | Volume – 6 | Issue – 4 | May-June 2022 Page 695
used which were an tificial or synthetic color and the
rest of the sample of candy from textile dyes.[11]
Food grade colors are not harmful for our ody if these
aren't used in excess amount ut colors are being used
indiscriminately l over Bangladesh and the European
ountries began the process of eliminating tificial food
colorings from foods.
The university of California San rancisco school of
Medicine showed that a ertain food coloring (caramel
food coloring) diminishes immune system function
which could alter the body's ability to fight off
infection and even causing cancer. Another study
revealed that ponceau 4R lowers the RBC and
haemoglobin concentration in body
bloodstream.[12,13]
Textile dyes can cause damage to liver, kidneys,
heart, skin, eyes, lungs and bones. It also results in
insufficient oxygen supply to skin and mucus
membranes along with degenerative changes in the
stomach, abdomen and testes. The intake of textile
due results in anaemia, nausea, constipation and
epigastricpain due to toxicity.
Conclusion
The study findings divulged a greater bulk of non
food grade colors in use at different food industries in
India. The proportion of minor degree of food grade
colors using in the industries is a bad news for the
consumers and the country as a whole. It's the
greatest public health threat. The GOI and different
volunteer international and national organizations
should take immediate bid to curb these ongoing
malpractices to get rid of food adulteration.[14]
References
[1] Alves S. P., Brum D. M., Branco de Andrade E.
C., Pereira Netto A. D., 2008, Determination of
synthetic dyes in selected foodstuffs by high
performance liquid chromatography with UV-
DAD detection. Food Chem.; 107(1):489–96.
[2] Anouar E. H., Osman C. P., Jean- Frédéric F
Weber, and Nor Hadiani Ismail N. H., 2014,
'UV/Visible spectra of a series of natural and
synthesised anthraquinones: experimental and
quantum chemical approaches' Springer plus; 3:
233.
[3] Ashfaq N. and Masud T., 2002 ' Surveillance
on Artificial Colours in Different Ready to Eat
Foods', Pakistan Journal of Nutrition 1 (5): 223-
225
[4] Bachalla N., 2016, ' Identification of synthetic
food colors adulteration by paper
chromatography and spectrophotometric
methods' IAIM; 3(6): 182-191.
[5] Anouar E. H., Osman C. P., Jean- Frédéric F
Weber, and Nor Hadiani Ismail N. H., 2014,
'UV/Visible spectra of a series of natural and
synthesised anthraquinones: experimental and
quantum chemical approaches' Springer plus; 3:
233.
[6] The Food (Colouring Substances) Regulations,
2011, in The Gazette of the Democratic
Socialist Republic of Sri Lanka Extraordinary,
No 1688/28 of January 14, 2011, Sri Lanka.
[7] Saleeml N., Umar1 Z. N., khanl S. I., 2013,
'survey on the use of synthetic food colors in
food samples procured from different
educational institutes of Karachi city', vol. 3,
no. 1, pp. 1 – 7.
[8] International Food Information Counsil (IFIC)
and U. S. Food and Drug Administration, 2010,
Food ingredients and colours.
[9] Padmaja R, Jonnalagadda PR, Ramesh V. B. A,
2004, Nadamuni Naidu Type, extent and use of
colours in ready-to-eat (RTE) foods prepared in
the non- industrial sector - a case study from
Hyderabad, Indian International Journal of
Food Science & Technology 39. (2): 125–131.
[10] IFIC, 2011. International Food information
council foundation. Food Survey.
[11] Farzianpour F., Khaniki J. G., Batebi F and
Yunesian M, 2012. Compare the effects of two
educational methods on the health principles
knowledge of employees in food preparation.
Am. J. Applied Sci., 9: 1678-1683 DOI: 10.
3844/ajassp. 2012. 1678. 1683
[12] Farzianpour F., Khaniki . J. G., Younesian M.,
Ghahferkhi B. B., Sadeghi M. and Hosseini S.,
2013, Evaluation of food color consumption
and determining color type by thin layer
chromatography, American Journal of Applied
Sciences, 10 (2): 172-178.
[13] Egan M. B., Raats M. M., Grubb S. M., Eves
A. and Lumbers M. L. et al., 2007. A review of
food safety and food hygiene training studies in
the commercial sector. Food Control, 18: 1180-
1190. DOI: 10. 1016/j. foodcont. 2006. 08. 001
[14] Koutsogeorgopoulou L, Maravelias C,
Methenitou G, Koutselinis A, 1998,
'Immunological aspects of the common food
colorants, Amaranth and Tartrazine',
Department of Forensic Medicine and
Toxicology. School of Medicine. Athens.
Greece.

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Analysis of Food Coloring

  • 1. International Journal of Trend in Scientific Research and Development (IJTSRD) Volume 6 Issue 4, May-June 2022 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470 @ IJTSRD | Unique Paper ID – IJTSRD50109 | Volume – 6 | Issue – 4 | May-June 2022 Page 693 Analysis of Food Coloring Aanchal Alok1 , Dr. H. L. Saxena2 1 Research Scholar, 1,2 Department of Chemistry, Himalayan University, Itanagar, Arunachal Pradesh, India ABSTRACT This study was conducted to bring to light food coloring used in three selected dishes. The dishes were taken naming ice-cream, candy and tomato sauce applying convenience sampling method. The study revealed to take part 78.4% non food grade and 21.5 % food grade colors in widespread use in these three dishes. Various textile dye and cosmetic color were in abundance used as non food grade colors and various synthetic colors e. g. amaranth red and ponceau 4R were as food grade colors in the selected dishes. KEYWORDS: Food Coloring, Dishes, Food Grade Colors, Non Food Grade Colors How to cite this paper: Aanchal Alok | Dr. H. L. Saxena "Analysis of Food Coloring" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456- 6470, Volume-6 | Issue-4, June 2022, pp.693-695, URL: www.ijtsrd.com/papers/ijtsrd50109.pdf Copyright © 2022 by author(s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0) 1. General Background Food coloring is the dye, pigment or substances adhered to foods or drinks in order to alter the color or the trick to sustain the prevailing colors with attractive flavors to gain affluent aesthetic look. Food coloring is available in liquids, gels, powders and pastes forms to be used both in commercial food production and in domestic cooking. Food coloring is used in a variety of non food applications including cosmetics, home crafts, pharmaceuticals and medical devices by din of their safety and general availability.[1,2] The color additives main purpose is to seem more inviting and appealing, especially to the children. The color in foods losses due to exposure to light, air, temperature, moisture and storage conditions. It corrects natural color variations and enhances natural existing colors. It provides color to colorless and "fun" foods. Food color has always been a value of quality. Without color additives, margarine won't be yellow, colas won't be brown and mint ice-cream won't be green. Color additives have come into fashion as vital part of all foods we eat. History The addition of colorant to foods is thought to have happened in Egyptian cities, where candy makers around 1500 BC added natural extracts and wine to improve product appearance. Up to the middle of the 19th century ingredients such as the spice saffron from the local areas to the production units were added for decorative effect to certain foodstuffs. In 1856, the first synthetic color (mauvine) was developed by Sir William Henry Perkin and by the turn of the century, unmonitored color additives had spread through the USA and Europe in all the source of popular foods, including mustard, jellies, wine and ketchup. Sellers at the time offered more than 80 artificial coloring agents, some intended for dyeing textiles, not foods. As the 1900s starting, the bulk of chemically synthesized colors were derived from aniline, a petroleum product in toxic nature. Chemically synthesized colors were easier to produce, less expensive and superior in coloring properties.[3,4] Type of clolors The food permitted colors are some naturally derived colors, principally plant extracts, their chemically identical synthetic duplicates and chemically IJTSRD50109
  • 2. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD50109 | Volume – 6 | Issue – 4 | May-June 2022 Page 694 synthesized compounds. The coloring matters gone to foods may be grouped into main 2 classes; natural and synthetic or artificial colors by name. Natural colors are approved for foods include carmines or cochineal (red), carotene (orange red), saffron (orange), anthocyanin (blue), curcumin (orange and yellow),chlorophyll (green), annatto (orange) and caramel (dark brown) etc. Synthetic or artificial colors (some are adopted worldwide) include, Red colorants: Amaranth, carmoisine, erythrosine, fast red E, ponceau 4R and allura red AC. 1. Orange or yellow colorants: Tartazine, yellow 2G, sunset yellow FCF. 2. Blue colorants: Brilliant blue FCF, Indigo carmine. 3. Green colorants: Green FCF, Fast green S .[5,6] Health Impact The approved colors aren't always safe. Moreover, even the safest permitted color, if taken in excessive range proved detrimental for human health. Since synthetic dyes contained various heavy metals like Pb, Hg, As, Cu, Ni, Mg, Co etc., various studies have found that it can cause hyperactivity and lapses of concentration in children. Coloring also cause triggers hyperactivity in some sensitive children, reduce liver and duct activity, rises thyroid tumors, increase allergy-like reactions primarily in aspirin- sensitive fellows, causes tumors of adrenal gland and kidney, carcinogenic and finally death. Eating foods containing industrial dyes and colors causes violent allergic reactions, respiratory problems, asthma, liver disorders and kidney dysfunction and bone marrow disorders. Food, as a basic need for all people must become wholesome and safe. It's only nutritive when it is pure, fresh and free from hazardous matters. Food coloring is a premier public hazard affecting the quality of life. The nature of food coloring varies from place to place. Due to illiteracy and poor knowledge on food colors, many industries use banned and synthetic or artificial colors to produce a lot of food in the country. Considering the various toxic effects of colors, the current study was planned to survey on various food color materials, including the banned ones (textile or cosmetic colors), natural, artificial in terms of development, regulations and technical regulations.[7,8] Framework Study Nature The study was an observational study. Sample Size and Information Collection There were framed a total of 40 samples from 20 food industries to carry on the study. Industries' owners, labors and food vendors helped to collect the information in this connection. Questionnaire Development A standard questionnaire was developed to gain the relevant information on the study. The query were designed, pretested, modified and resettled to achieve the recorded information easily. Data Aggregation Data was aggregated by asking question to the factory owners, labors, respective produces venders and color sellers in the market.[9,10] Data Verification Questionnaire were checked each day after interviewing and again after completion of data aggregation and coded before entering into computer technology. The data was edited if there was any discrepancy (doubt entry, wrong entry etc.). Statistical Analysis All the statistical analysis and all other data processing were done by using specialized analytical software program. Microsoft Word and Microsoft Excel were in frequent application on tabular, chart and graphical representation. All the statistical analysis were done very carefully in order to obtain the good accuracy of result. Results The study on colors using in selected 40 samples of three produces was conducted. Table 1 represented the different colors used in total 20 industries' 40 food samples off selected three dishes. According to the value of table 1, it was showed in items of ice-cream that 42% red, 35% green and 23% yellow, the percentage of panceau 4R and red color was in candy making was 77% and 23%. In Tomato sauce's amaranth red color. The food grade colors were found only in 10 and the rest 30 items were made from non food grade colors. The figure 1 represented the percentage of non food grade and food grade colors used in producing ice- cream, candy and tomato sauce was found as 78.6% and 21.5% respectively. Discussion From the results, it is observed that yellow, red and green colors were used for e production of ice-cream which is recognized as textile dye otherwise cosmetic olors due to the ignorance and illiteracy of e manufacturers making the ultimate ictimization to the consumers. This scene as not only common in case of ice cream ut also hawaii methai and particularly andy. In candy production procedure, onceau 4R partially
  • 3. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD50109 | Volume – 6 | Issue – 4 | May-June 2022 Page 695 used which were an tificial or synthetic color and the rest of the sample of candy from textile dyes.[11] Food grade colors are not harmful for our ody if these aren't used in excess amount ut colors are being used indiscriminately l over Bangladesh and the European ountries began the process of eliminating tificial food colorings from foods. The university of California San rancisco school of Medicine showed that a ertain food coloring (caramel food coloring) diminishes immune system function which could alter the body's ability to fight off infection and even causing cancer. Another study revealed that ponceau 4R lowers the RBC and haemoglobin concentration in body bloodstream.[12,13] Textile dyes can cause damage to liver, kidneys, heart, skin, eyes, lungs and bones. It also results in insufficient oxygen supply to skin and mucus membranes along with degenerative changes in the stomach, abdomen and testes. The intake of textile due results in anaemia, nausea, constipation and epigastricpain due to toxicity. Conclusion The study findings divulged a greater bulk of non food grade colors in use at different food industries in India. The proportion of minor degree of food grade colors using in the industries is a bad news for the consumers and the country as a whole. It's the greatest public health threat. The GOI and different volunteer international and national organizations should take immediate bid to curb these ongoing malpractices to get rid of food adulteration.[14] References [1] Alves S. P., Brum D. M., Branco de Andrade E. C., Pereira Netto A. D., 2008, Determination of synthetic dyes in selected foodstuffs by high performance liquid chromatography with UV- DAD detection. Food Chem.; 107(1):489–96. [2] Anouar E. H., Osman C. P., Jean- Frédéric F Weber, and Nor Hadiani Ismail N. H., 2014, 'UV/Visible spectra of a series of natural and synthesised anthraquinones: experimental and quantum chemical approaches' Springer plus; 3: 233. [3] Ashfaq N. and Masud T., 2002 ' Surveillance on Artificial Colours in Different Ready to Eat Foods', Pakistan Journal of Nutrition 1 (5): 223- 225 [4] Bachalla N., 2016, ' Identification of synthetic food colors adulteration by paper chromatography and spectrophotometric methods' IAIM; 3(6): 182-191. [5] Anouar E. H., Osman C. P., Jean- Frédéric F Weber, and Nor Hadiani Ismail N. H., 2014, 'UV/Visible spectra of a series of natural and synthesised anthraquinones: experimental and quantum chemical approaches' Springer plus; 3: 233. [6] The Food (Colouring Substances) Regulations, 2011, in The Gazette of the Democratic Socialist Republic of Sri Lanka Extraordinary, No 1688/28 of January 14, 2011, Sri Lanka. [7] Saleeml N., Umar1 Z. N., khanl S. I., 2013, 'survey on the use of synthetic food colors in food samples procured from different educational institutes of Karachi city', vol. 3, no. 1, pp. 1 – 7. [8] International Food Information Counsil (IFIC) and U. S. Food and Drug Administration, 2010, Food ingredients and colours. [9] Padmaja R, Jonnalagadda PR, Ramesh V. B. A, 2004, Nadamuni Naidu Type, extent and use of colours in ready-to-eat (RTE) foods prepared in the non- industrial sector - a case study from Hyderabad, Indian International Journal of Food Science & Technology 39. (2): 125–131. [10] IFIC, 2011. International Food information council foundation. Food Survey. [11] Farzianpour F., Khaniki J. G., Batebi F and Yunesian M, 2012. Compare the effects of two educational methods on the health principles knowledge of employees in food preparation. Am. J. Applied Sci., 9: 1678-1683 DOI: 10. 3844/ajassp. 2012. 1678. 1683 [12] Farzianpour F., Khaniki . J. G., Younesian M., Ghahferkhi B. B., Sadeghi M. and Hosseini S., 2013, Evaluation of food color consumption and determining color type by thin layer chromatography, American Journal of Applied Sciences, 10 (2): 172-178. [13] Egan M. B., Raats M. M., Grubb S. M., Eves A. and Lumbers M. L. et al., 2007. A review of food safety and food hygiene training studies in the commercial sector. Food Control, 18: 1180- 1190. DOI: 10. 1016/j. foodcont. 2006. 08. 001 [14] Koutsogeorgopoulou L, Maravelias C, Methenitou G, Koutselinis A, 1998, 'Immunological aspects of the common food colorants, Amaranth and Tartrazine', Department of Forensic Medicine and Toxicology. School of Medicine. Athens. Greece.