thank you The structure of the T4 virus we are using in this lab is pretty complex. The virus capsid is made of more than 25 different proteins. It contains an icosahedral head and a tail. The tail consists of a helical tube with an endplate to which long tail fibers are attached. The genome of bacteriophage T4 is a double-stranded linear DNA molecule 168,903 base pairs in length, with repeated sequences at the ends, called terminal repeats, which include single-stranded termini, Because of these terminal repeats, the DNA molecules anneal to each other forming long concatemers. The concatemers are cut at random sites in the packaging process of the genome inside the virus head, resulting in permutations of the sequences in the DNAs of different viral particles. When phage particles come in contact with a bacterial cell, they adsorb to the cell's surface and inject their DNA into the cell. Soon after infection there is transcription of some phage genes in the bacterial cell leading to the production of enzymes needed for (1) the replication of the phage DNA and (2) transcription of additional phage genes. Later on, transcription of genes encoding the head and tail proteins takes place. The capsid proteins assemble around the phage DNA molecules, forming progeny bacteriophage particles. The bacterial cell eventually lyses releasing many infectious viral particles. These can infect adjacent cells and lyse them too. Many rounds of infection, result in a clear spot in a bacterial lawn, corresponding to the place where the bacterial cells have been lysed by the phage. This clear spot is called a plaque. Each original phage will produce a plaque. When you count plaques produced by the T4 phoge you are counting the original number of phages or what are called plaque forming units: pfu. Materials, reagents and equipment E. coliculture growing exponentially Phoge(T4) stock Sterile SM (a solution containing sodium chloride, magnesium sulfate, Tris.

1. thank you
The structure of the T4 virus we are using in this lab is pretty complex. The virus capsid is made
of more than 25 different proteins. It contains an icosahedral head and a tail. The tail consists of
a helical tube with an endplate to which long tail fibers are attached. The genome of
bacteriophage T4 is a double-stranded linear DNA molecule 168,903 base pairs in length, with
repeated sequences at the ends, called terminal repeats, which include single-stranded termini,
Because of these terminal repeats, the DNA molecules anneal to each other forming long
concatemers. The concatemers are cut at random sites in the packaging process of the genome
inside the virus head, resulting in permutations of the sequences in the DNAs of different viral
particles. When phage particles come in contact with a bacterial cell, they adsorb to the cell's
surface and inject their DNA into the cell. Soon after infection there is transcription of some
phage genes in the bacterial cell leading to the production of enzymes needed for (1) the
replication of the phage DNA and (2) transcription of additional phage genes. Later on,
transcription of genes encoding the head and tail proteins takes place. The capsid proteins
assemble around the phage DNA molecules, forming progeny bacteriophage particles. The
bacterial cell eventually lyses releasing many infectious viral particles. These can infect adjacent
cells and lyse them too. Many rounds of infection, result in a clear spot in a bacterial lawn,
corresponding to the place where the bacterial cells have been lysed by the phage. This clear spot
is called a plaque. Each original phage will produce a plaque. When you count plaques produced
by the T4 phoge you are counting the original number of phages or what are called plaque
forming units: pfu. Materials, reagents and equipment E. coliculture growing exponentially
Phoge(T4) stock Sterile SM (a solution containing sodium chloride, magnesium sulfate, Tris