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Workshop jaca spain 2014_alfredo garcia fernandez

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Workshop jaca spain 2014_alfredo garcia fernandez

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Workshop jaca spain 2014_alfredo garcia fernandez

  1. 1. AdAptA project: Population size and population origin for marginal/peripheral populations •Alfredo García Fernández •Carlos Lara-Romero •Javier Morente-López •Mª Luisa Rubio Teso •Jose Carmen Soto Correa •José M. Iriondo Alegría •COST ACTION MaP JACA 2014.
  2. 2. Ideas & Objetives Designs & Experiments QueriesIntroduction • High mountain ecosystems, highly endangered by global change. • For many alpine species, Mediterranean mountains are southern limit of distribution (glacial refugee). • Upward/latitudinal migration is not an option.
  3. 3. Ideas & Objetives Designs & Experiments QueriesIntroduction • High mountain ecosystems, highly endangered by global change. • For many alpine species, Mediterranean mountains are southern limit of distribution (glacial refugee). • Upward/latitudina migration is not an option. • Adaptation as one of the main response against new environmental conditions.
  4. 4. Ideas & Objetives Designs & Experiments QueriesIntroduction • High mountain ecosystems, highly endangered by global change. • For many alpine species, Mediterranean mountains are southern limit of distribution (glacial refugee). • Upward migration is not an option. • Adaptation as one of the main response against new environmental conditions. • Introduction of “altitude variable” in the consideration of Central - Periphery populations e.g. One population in the southern distribution limit but at intermediate altitude could be considered marginal population? Local gradient VS Whole distribution.
  5. 5. Silene ciliata Pourret • Perennial cushion plant. • Mediterranean mountain plant • Central System (Central Iberian Peninsula) southern limit of species distribution. • Small occupation area. Certain population isolation. • Inhabit altitude range from 1900 to 2500 m.a.s.l. o High phenotypic variability o Population genetic o Limited dispersal availability o Demographic fitness Ideas & Objetives Designs & Experiments QueriesIntroduction Not a forest species
  6. 6. Ideas & Objetives Designs & Experiments QueriesIntroduction Importance of gene flow and population size in evolutionary potential in marginal populations FITNESS Evolutive potentialGenetic diversity Marginal populations Gene flow between populations of different size. BIG/SMALL (Lemu & Fischer 2008) Natural selection Gene flow between populations CENTRER/PERIPHERY (Sexton et. al. 2011) ? ?
  7. 7. OBJETIVES Evaluate the effects of gene-flow between Center-Periphery VS Periphery-Periphery and population size in the biological performance of individuals from marginal populations of S. ciliata. QUESTIONS: (1) Can be improved the viability of marginal populations by gene flow with pollen from populations that occur in the environmental optimum conditions of the species? OR MAYBE (2) Could be more effective the gene flow with pollen from other marginal populations that are under similar selective pressures? AND ALSO (3) What is the influence of population size in the population viability? Ideas & Objetives Designs & Experiments QueriesIntroduction
  8. 8. Definition of centrer and periphery A critical step for our approach is the definition of center and periphery populations Ideas & Objetives Designs & Experiments QueriesIntroduction Ávila SegoviaSalamanca Madrid
  9. 9. • Environmental point of view instead of geographic • MAXENT approach with environmental values that explain the species distribution. • Identification of center and periphery populations Definition of centrer and periphery A critical step for our approach is the definition of center and periphery populations Ideas & Objetives Designs & Experiments QueriesIntroduction
  10. 10. GuadarramaGredosBéjar • Central Big Population • Peripheric Big Population • Peripheric Small Population EXPERIMENT 1: 1. In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra- populations). Ideas & Objetives Designs & Experiments QueriesIntroduction
  11. 11. Planteamiento Diseño PreguntasIntroducción Periphery Big from other mountain range Periphery Small A Central Big A Periphery Big A • Each periphery population received pollen from itself, from the central of its same mountain range and from other peripheral of the same or bigger population size. Experiment 1: In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra-populations). Periphery effects
  12. 12. • Plant cuttings from 20 mother plants in each population Experiment 1: In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra-populations). • Manual crossings. • Seed sowing in situ in periphery populations: • 3 types of crossings. • Control of intrapopulation variability. • Block design to avoid environmental heterogeneity • Production of clonal families from cuttings. Ideas & Objetives Designs & Experiments QueriesIntroduction 180 mother plants! 14400 cuttings!!! 18000 crosses!!!
  13. 13. Experiment 1: In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra-populations). Ideas & Objetives Designs & Experiments QueriesIntroduction 24000 SEEDS !!!!!!
  14. 14. Béjar Guadarrama Gredos ¿? ¿? ¿? Experiment 2: In-situ Common Garden essays using seeds from manual crossings (Inter- and Intra-populations). Full effects. Ideas & Objetives Designs & Experiments QueriesIntroduction Periphery Small Central Big Periphery Big Periphery Small Central Big Periphery Big Periphery Small Central Big Periphery Big
  15. 15. Experiment 3: Transcriptome comparisons during the seedling stage Ideas & Objetives Designs & Experiments QueriesIntroduction Periphery population Central population Seed collection Greenhouse sowing ARN extraction and sequencing Plant ARN after drought treatment Plant ARN control
  16. 16. Some ideas… • Experiment 1: Common garden approach, Periphery effects: Sowing design. • Experiment 2: Common garden approach, Full effects: Following the same design as experiment 1, > 50,000 seeds. •Experiment 3: Transcriptome approach: Experience in NGS? Drought treatment? Pool individuals Ideas & Objetives Designs & Experiments QueriesIntroduction
  17. 17. Thanks for your attention
  18. 18. AdAptA project: Population size and population origin for marginal/peripheral populations •Alfredo García Fernández •Carlos Lara-Romero •Javier Morente-López •Mª Luisa Rubio Teso •Jose Carmen Soto Correa •José M. Iriondo Alegría •COST ACTION MaP JACA 2014.

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