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Symposium porto 2013 alfredo garcia fernandez

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Symposium porto 2013 alfredo garcia fernandez

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Symposium porto 2013 alfredo garcia fernandez

  1. 1. Local adaptation in marginal high mountain populations A. García Fernández 1,2, J. Morente 1, C. Lara-Romero 1 , M.L. Rubio 1 & J. M. Iriondo 1 1 Universidad Rey Juan Carlos, Móstoles, Madrid, Spain. 2 Institut Botanic de Barcelona, I.C.U.B.-C.S.I.C., Barcelona, Spain
  2. 2. Mediterranean mountains: a) Local biodiversity. b) High number of endemics. • Unfavorable periods. • Altitude gradient: a) Temperatures. b) Precipitations. c) Soil properties. d) Vegetal community. Study zone Local adaptation in marginal high mountain populations.
  3. 3. WinterSpringSummer Study zone Local adaptation in marginal high mountain populations.
  4. 4. Study zone Local adaptation in marginal high mountain populations. Model species: Silene ciliata: • Mediterranean mountain ranges. • Rear margin distribution. • Facultative outcrosser. • Several levels of ploidy. • Late flowering.
  5. 5. Intermediate population Low population High population Gimenez-Benavides et al. (2007) New Phyto. Study zone What do we know? Floral asynchrony Local adaptation in marginal high mountain populations.
  6. 6. Intermediate population Low population High population Study zone What do we know? Floral asynchrony Local adaptation Gimenez-Benavides et al. (2007) Ann. Bot. Local adaptation in marginal high mountain populations.
  7. 7. Study zone What do we know? Floral asynchrony Local adap. Drought resistance García-Fernández et al. (2012) Plant Bio. Local adaptation in marginal high mountain populations. Fv/Fmphtosynteticstatus Days without water supply Low pop. Inter. pop. High pop.
  8. 8. Study zone What do we know? Floral asynchrony Local adap. Drought resistance Inbreeding García-Fernández et al. (2012) Oikos Local adaptation in marginal high mountain populations. High Pop. Interm. Pop. Low Pop. Surveys %Survival Self-cross Within-cross Betw-cross
  9. 9. Study zone What do we know? AdAptA New project: Evaluate the importance of peripheral populations vs. central populations. • Population size. • Genetic diversity. • Gene flow. • Potential adaptation. Local adaptation in marginal high mountain populations. Altitude Ecker et al. (2008) Mol. Eco.
  10. 10. Study zone What do we know? AdAptA New project: Questions: 1. Is there local adaptation between central and peripherial populations? How important is peripheral population size? Local adaptation in marginal high mountain populations.
  11. 11. Study zone What do we know? AdAptA New project: Questions: 2. Which gene flow is more benefical for peripheral popualtions, central- peripheral or peripheral-peripheral? Local adaptation in marginal high mountain populations.
  12. 12. Study zone What do we know? AdAptA New project: Questions: 3. Can local adaptation be detected through differential gene expression? Local adaptation in marginal high mountain populations.
  13. 13. Study zone What do we know? AdAptA Experiments Experiment 1: Population size effect 1. Generate F1 in greenhouse to avoid maternal effects. 2. Set seeds from manual pollinations, F2. 3. Reciprocal sowing and monitoring. Local adaptation in marginal high mountain populations.
  14. 14. Study zone What do we know? AdAptA Experiments Experiment 2: Gene flow C-P vs. P-P 1. Generate F1 in greenhouse to avoid maternal effects. 2. Seeds from manual pollinations, F2. All crossings. 3. Reciprocal sowing and monitoring. Local adaptation in marginal high mountain populations.
  15. 15. Study zone What do we know? AdAptA Experiments Experiment 3: Transcriptome analysis 1. Generate F1 from central and marginal populations. 2. Sow in low altitude peripheral location or similar artificial conditions. 3. Analyze seedling transcriptome, Illumina. Local adaptation in marginal high mountain populations.Blavet et al. (2011) BMC Genomics
  16. 16. Study zone What do we know? AdAptA Experiments Questions Experiment 3: Transcriptome analysis • Best conditions to grow seedlings? • Next Generation Sequencing Plataform? Local adaptation in marginal high mountain populations. Ex situ Greenhouse In situ Field sowing In situ Jiffy pots

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