This presentation covers various Techniques & Technologies of Biology Life Science

1. • Presented by :-
• Mr. Vishal Rathod
K. K. Wagh College of Agricultural Biotechnology, Nashik
Biotechniques & Biotechnologies

3. Biotechnologies
-Bioengineering
-Biomedicine
-Biopharmaceutics
-Bioinformatics
-Bionics
-Bioremediation
-Genetic Engineering
-Hybridization
-Selective Breeding
-Human Cloning
-Plant Tissue Culture
-Nanomedicine

4. Biotechniques
-Recombinant DNA Technology
-Genetic Engineering
-Blotting Techniques
-Electrophoresis Techniques
-Gene Transformation
Techniques
-DNA Sequencing Techniques
-RNA Sequencing Techniques
-Gene Addition-Deletion-
Substitution Techniques
-Probing / Hybridization Techniques
-DNA Fingerprinting Techniques
-Foot Fingerprinting Techniques
-Molecular Marking Techniques
-Screening Techniques
- CRISPR Cas 9 Technique

5. 1. Recombinant DNA Technology
- DNA Cloning
- Expression of Gene or DNA
- Reverse Transcription
- Genomic Libraries
- cDNA Libraries
- Transformation Experiments
- Molecular Markers

6. A. DNA Cloning or Gene Expression
- By using RDT, The desired segment of DNA or gene is being cloned
& expressed for human welfare…

7. B. Genomic Libraries & cDNA Libraries
-The RDT is being used to produce Genomic & cDNA Libraries which aims at
storage and preservation elite genes & germplasm of plant & animal species…

8. C. Molecular Markers
- Molecular Markers are the specific base sequences present on a DNA which
are referred to locate an another base sequence on that DNA…

9. D. Reverse Transcription
- The Reverse Transcription is the process in which the DNA is prepared by
using RNA as a template with the help of Reverse Transcriptase…

10. 2. Blotting Techniques
- Southern blotting technique
- Northern blotting technique
- Western blotting technique

11. A. Southern Blotting Technique
- Southern Blotting is the technique used for separation & detection of DNA
bands over a gel on the basis of molecular weight & charge present on them…

12. B. Northern Blotting Technique
- Northern Blotting is the technique used for separation & detection of RNA
bands over a gel on the basis of molecular weight & charge present on them…

13. C. Western Blotting Technique
- Western Blotting is the technique used for separation & detection of protein
molecules over a gel on the basis of molecular weight & charge present on them…

14. 3. Gene Transformation Techniques
A. Indirect Gene Transformation
- Agrobacterium mediated gene Transformation
- Agro-infection
B. Direct Gene Transformation
- Electroporation
- Chemoporation
- Microinjection
- Lipofection
- Pollen Transformation
- Particle Gun Method
- Fibre Mediated DNA delivery
- Laser Induced DNA delivery

15. A. Indirect Gene Transformation
- In this method, a gene is transformed into host by using an intermediate
agent like agrobacterium…

16. B. Direct Gene Transformation
- In this method, a gene is transformed into host without using any intermediate
agent but induced by physical or chemical materials…

17. 4. DNA Sequencing
- Chemical Method / Maxam & Gilbert Method
- Enzymatic Method / Sanger Coulson Method
- Pyrosequencing
- Automated DNA Sequencing
- Primer Walking
- RNA Sequencing

18. A. Chemical Method of DNA Sequencing
- In this method, The DNA is sequenced by using some chemicals i.e. chemically
prepared bases…

19. B. Enzymatic Method of DNA Sequencing
- In this method, The DNA is sequenced by using some enzymes like restriction
enzymes, polymerases, etc…

20. 5. Other Bio-Techniques
- Artificial Chromosome
- Exome Sequencing
- Gene Addition – Deletion – Substitution
- Probing / Hybridization
- ELISA
- Nick Translation & End Filling
- DNA Fingerprinting
- Foot Fingerprinting
- CRISPR Cas9 Technique
- Colony Hybridization
- Cytological Hybridization