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Test For
Identification of
Type of Emulsion
Prepared By:
Lect. Mr. S. S. Mistree
Sub:-Pharmaceutic’s-II
Ishwar Deshmukh Institute of Pharmacy, Digras
Yavatmal-445203
2019-20
The following test are done to discriminate between O/W
and W/O emulsion
1. Dilution Test
2. Dye Test
3. Conductivity Test
4. Fluorescence Test
Dilution Test:
 The emulsion can identify on the basis of dilution test
Emulsion is diluted with water/oil
O/W emulsion easily diluted with water
W/O emulsion easily diluted with oil
 Dye Test:
 when an emulsion is mixed with a oil soluble dye such as scarlet
red dye and observed under the microscope.
 If the continuous phase shows red color and dispersed globules
shows colorless means W/O type of emulsion
 If the dispersed globules shows red color and continuous phase
colorless, means O/W type of emulsion
Conductivity Test:
Water is good conductor of electricity but oil is not
This test perform by dipping pair of electrode connected by a bulb in
the emulsion
If the bulb glow that means O/W type of emulsion
If not glow that means W/O type of emulsion
Fluorescence Test:
Certain fixed oil has property of fluorescing in presence of UV
radiation on microscopic observation.
If continuous phase give fluorescence that means W/O type of
emulsion.
If dispersed globules gives fluorescence that means O/W type of
emulsion.
Thank you…
References:
R. M. Mehta, pharmaceutics-II, third eddition-2010,vallabh
prakashan.

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Test for identification of type of emulsion

  • 1. Test For Identification of Type of Emulsion Prepared By: Lect. Mr. S. S. Mistree Sub:-Pharmaceutic’s-II Ishwar Deshmukh Institute of Pharmacy, Digras Yavatmal-445203 2019-20
  • 2. The following test are done to discriminate between O/W and W/O emulsion 1. Dilution Test 2. Dye Test 3. Conductivity Test 4. Fluorescence Test
  • 3. Dilution Test:  The emulsion can identify on the basis of dilution test Emulsion is diluted with water/oil O/W emulsion easily diluted with water W/O emulsion easily diluted with oil
  • 4.  Dye Test:  when an emulsion is mixed with a oil soluble dye such as scarlet red dye and observed under the microscope.  If the continuous phase shows red color and dispersed globules shows colorless means W/O type of emulsion  If the dispersed globules shows red color and continuous phase colorless, means O/W type of emulsion
  • 5. Conductivity Test: Water is good conductor of electricity but oil is not This test perform by dipping pair of electrode connected by a bulb in the emulsion If the bulb glow that means O/W type of emulsion If not glow that means W/O type of emulsion
  • 6. Fluorescence Test: Certain fixed oil has property of fluorescing in presence of UV radiation on microscopic observation. If continuous phase give fluorescence that means W/O type of emulsion. If dispersed globules gives fluorescence that means O/W type of emulsion.
  • 7. Thank you… References: R. M. Mehta, pharmaceutics-II, third eddition-2010,vallabh prakashan.