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Fermentation Examples
BY- SANCHIT
DHANKHAR
History of Fermentation
Age old applications:
1. Wine/ Beer/ Spirits
2. Cheese and Yoghurt
Louis Pasteur:
 Hypothesised that bacteria spoils wine
 Suggested that wine be heated to kill bacteria
 Hence: pasteurization of milk
Fermentation’s
Pros and Cons
 Extended shelf life of
food (ex. Cheese)
 Eases Digestion (ex.
Wild rice)
 New [better] flavours (ex.
Chocolate)
 Can be unpredictable
(i.e. bad bacteria win the
battle)
 New [worse] flavours
(ex. Mouldy bread tastes
terrible)
The Process
 Aerobic respiration
 release of energy from
glucose or another
organic substrate in the
presence of Oxygen
 CO2, H2O, an energy
produced
 Anaerobic respiration
 release of energy from
glucose or another
organic substrate in the
absence of Oxygen
 Products: CO2, energy,
and alcohol or various
organic acids
Fermentation Definition:
Anaerobic respiration of food by
micro organisms
Types of fermentation:
1. Bacterial fermentation
2. Yeast fermentation
3. Mold and Enzyme fermentation
1. Bacterial Fermentation (4 types)
a) Lactic Acid Bacteria
(pickles, sauerkraut)
b) Acetic Acid Bacteria
(vinegar)
c) Carbon Dioxide
Bacteria (Edam, Gouda,
Swiss)
d) Proteolytic Bacteria
(cocoa, chocolate)
bacteria
2. Yeast Fermentation
 Glu Ethyl alcohol +
CO2
 Best temperature: 27
degree C (warm)
 Other sugars will ferment
(mal, suc, fru)
 Too much salt ruins the
process
When baking: follow the
recipe
3. Mold and Enzyme Fermentation
 Enzymes in Mold can be useful:
-Break down cellulose thus grains easier to
chew
-Add flavour and texture to cheeses (ex- blue)
Wine
 Dates back to Middle
east 3000 bc
 Fermentation of grapes
 Scientific process yet so
many variables
 Growing years affect
vintages
Coffee
 Coffee beans fermented
by bacteria and
enzymes (2 methods):
1. Wet Method: soaked for
12-24 hours and dried
2. Dry Method: washed
then dried for 2-3 weeks
Tea
 3000 AD (at the latest)-
Cultivated in China
 Rolled leaves begin to
ferment
 Lets stand at 27 degree
C for 2-3 hrs
 Types: Green, Oolong,
Black
Chocolate
 1.5 million tons cocoa
produced each year
 Supply: W. Africa
Produced: S. America
 Enzyme fermentation in
the sun via proteolytic
bacteria
 Bitter beans become
sweeter and brown
THANKYOU

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Examples of fermentation- Pharmaceutical Microbiology

  • 2. History of Fermentation Age old applications: 1. Wine/ Beer/ Spirits 2. Cheese and Yoghurt Louis Pasteur:  Hypothesised that bacteria spoils wine  Suggested that wine be heated to kill bacteria  Hence: pasteurization of milk
  • 3. Fermentation’s Pros and Cons  Extended shelf life of food (ex. Cheese)  Eases Digestion (ex. Wild rice)  New [better] flavours (ex. Chocolate)  Can be unpredictable (i.e. bad bacteria win the battle)  New [worse] flavours (ex. Mouldy bread tastes terrible)
  • 4. The Process  Aerobic respiration  release of energy from glucose or another organic substrate in the presence of Oxygen  CO2, H2O, an energy produced  Anaerobic respiration  release of energy from glucose or another organic substrate in the absence of Oxygen  Products: CO2, energy, and alcohol or various organic acids
  • 5. Fermentation Definition: Anaerobic respiration of food by micro organisms Types of fermentation: 1. Bacterial fermentation 2. Yeast fermentation 3. Mold and Enzyme fermentation
  • 6. 1. Bacterial Fermentation (4 types) a) Lactic Acid Bacteria (pickles, sauerkraut) b) Acetic Acid Bacteria (vinegar) c) Carbon Dioxide Bacteria (Edam, Gouda, Swiss) d) Proteolytic Bacteria (cocoa, chocolate) bacteria
  • 7. 2. Yeast Fermentation  Glu Ethyl alcohol + CO2  Best temperature: 27 degree C (warm)  Other sugars will ferment (mal, suc, fru)  Too much salt ruins the process When baking: follow the recipe
  • 8. 3. Mold and Enzyme Fermentation  Enzymes in Mold can be useful: -Break down cellulose thus grains easier to chew -Add flavour and texture to cheeses (ex- blue)
  • 9. Wine  Dates back to Middle east 3000 bc  Fermentation of grapes  Scientific process yet so many variables  Growing years affect vintages
  • 10. Coffee  Coffee beans fermented by bacteria and enzymes (2 methods): 1. Wet Method: soaked for 12-24 hours and dried 2. Dry Method: washed then dried for 2-3 weeks
  • 11. Tea  3000 AD (at the latest)- Cultivated in China  Rolled leaves begin to ferment  Lets stand at 27 degree C for 2-3 hrs  Types: Green, Oolong, Black
  • 12. Chocolate  1.5 million tons cocoa produced each year  Supply: W. Africa Produced: S. America  Enzyme fermentation in the sun via proteolytic bacteria  Bitter beans become sweeter and brown