Gross Anatomy and Histology of Tongue by Dr. Rabia Inam Gandapore.pptx
Ch12
1. Haemostatic parameters in chronic gastric
ulcers in rats.
Animal manipulations
Male albino rats weighing 200–250 g were used in this study. All animals were given free
access to food and water. The protocol for this project was approved by the Ethics Committee
of the University, and all experiments were performed according to the institutional guidelines
for animal care and use. The rats were divided into 16 groups randomly; each group consisted
of 6–7 rats. Laparotomy was performed under general anesthesia induced by an ip injection of
ketamine HCl (50 mg/kg) and xylazine HCl (10 mg/kg).
Measurement of gastric mucosal lesions
After collecting serum samples, the stomachs were removed and inflated by injection of 10 ml
of formalin 2% to fix the inner layers of the gastric wall. After 20 min, the stomachs were
incised along the greater curvature [19]. The area of the erosions in mm2 was calculated by
measuring the width and length of erosions.
19. Hara N, Hara Y, Natsume Y, Goto Y: Gastric hyperacidity and mucosal damage caused by
hypothermia correlate with increase in GABA concentrations of the rat brain. Eur J Pharmacol, 1991,
194, 77–81.
Statistical analysis
All data are presented as the mean ± SEM. Statistical evaluation of the data was performed
with an analysis of variance (ANOVA) followed by the SPSS, and a p-value less than 0.05 was
considered statistically significant
1
![Haemostatic parameters in chronic gastric
ulcers in rats.
Animal manipulations
Male albino rats weighing 200–250 g were used in this study. All animals were given free
access to food and water. The protocol for this project was approved by the Ethics Committee
of the University, and all experiments were performed according to the institutional guidelines
for animal care and use. The rats were divided into 16 groups randomly; each group consisted
of 6–7 rats. Laparotomy was performed under general anesthesia induced by an ip injection of
ketamine HCl (50 mg/kg) and xylazine HCl (10 mg/kg).
Measurement of gastric mucosal lesions
After collecting serum samples, the stomachs were removed and inflated by injection of 10 ml
of formalin 2% to fix the inner layers of the gastric wall. After 20 min, the stomachs were
incised along the greater curvature [19]. The area of the erosions in mm2 was calculated by
measuring the width and length of erosions.
19. Hara N, Hara Y, Natsume Y, Goto Y: Gastric hyperacidity and mucosal damage caused by
hypothermia correlate with increase in GABA concentrations of the rat brain. Eur J Pharmacol, 1991,
194, 77–81.
Statistical analysis
All data are presented as the mean ± SEM. Statistical evaluation of the data was performed
with an analysis of variance (ANOVA) followed by the SPSS, and a p-value less than 0.05 was
considered statistically significant
1](https://image.slidesharecdn.com/ch12-150729130905-lva1-app6891/85/Ch12-1-320.jpg)
