Embark on a captivating journey through the fascinating realm of genetics with our latest PDF guide, "RAPD Unleashed." RAPD, or Random Amplified Polymorphic DNA, has emerged as a powerful tool in the hands of genetic explorers, and this document is your passport to understanding its secrets. Unleash the potential of genetic discovery with "RAPD Unleashed." Whether you're a seasoned geneticist or a curious learner, this PDF promises to make the complexities of RAPD accessible, revealing the genetic stories waiting to be told within our DNA. Let the exploration begin!

1. Randomly
Amplified
Polymorphic
DNA

2. • Genetic markers resulting from PCR amplification of genomic DNA
segments recognized by random primers of arbitrary nucleotide
sequence.
• primers usually 10 mers
• GC rich.
• annealing temperature: low 35° C.
• amplified products are detected in agarose gels

3. PCR
• Buffer (containing Mg++)
• Template DNA
• 2 Primers that flank the fragment
of DNA to be Amplified
• dNTPs
• Taq DNA Polymerase (or another
thermally stable DNA polymerase)
RAPD
• Buffer (containing Mg++) usually
high Mg++ concentrations are used
lowering annealing stringency
• Template DNA
• 1 short primer (10 bases)not
known to anneal to any specific
part of the template DNA
• dNTPs
• Taq DNA Polymerase (or another
thermally stable DNA polymerase)

6. Advantageous
• Primer sequences are synthesized at
random (does not require any
sequences information).
• No gel transfer or hybridization is
required.
• Fast (many of the steps are
automated ).
• Relatively inexpensive
• Highly variable.
• The amount of template DNA is
extremely small.
Disadvantages
• Markers are dominant ( Presence of a
band could mean the individual is
either heterozygous or homozygous
for the sequence--can’t tell which ).