project

1. KLE INSTITUTE OF TECHNOLOGY
HUBBALLI
DEPT. OF MECHANICAL ENGINEERING
“TEMPERATURE EFFECT ON
STERILIZER”
NAME: MANJUNATH GUDDAD USN:2KE19ME033
GUIDENCE BY: PROF. SUSHRUT HALEVADIMATH

2. OBJECTIVES
Sterilization is done
➢ To maintain sterile condition.
➢ To avoid contamination.
➢ Destroy pathogen and prevent their transmission.

3. INTRODUCTION
• Maintaining sterile condition is of utmost importance in every plant
pathology, microbiology, tissue culture, and molecular biology
laboratory. Frequently, some bacteria and fungi cause huge nuisance in
culture media or other in vitro works. If the culture media, working
surface or tools get contaminated, this can lead to an unsuccessful
experimentation or unreliable inferences from the research. Various
physical and chemical agents and techniques are employed for
sterilizing culture media, surfaces and instruments.

4. WORKING PRINCIPLE

5. METHODS OF STERELIZATION

6. MOIST/WET HEAT
• • Moist heat kills microorganism's spores and bacteria quicker than dry
heat because it can cause hydrolysis of proteins in addition to the simple
heat coagulation.
• • Heating in presence of water
• • Much greater heat content than dry heat.
• • Used for culture media, liquid, glass with rubber or metal parts, filters,
infected materials, surgical gloves, dressing, linen, etc.

7. ADAVANTAGES
1. A dry heat cabinet is easy to install and has relatively low operating
costs;
2. It penetrates materials
3. It is nontoxic and does not harm the environment;
4. And it is noncorrosive for metal and sharp instruments.

8. DISADAVNTAGES
1. Time-consuming method because of a slow rate of heat
penetration and microbial killing.
2. High temperatures are not suitable for most materials e.g. plastic and
rubber items cannot be dry-heat sterilized because temperatures used
(160–170°C) are too high for these materials.
3. The time and temperature required will vary for different substances and
overexposure may ruin some substances.

9. CONCLUSION
• Despite using highly effective methods for sterilization by the
experienced researcher, however, the contamination in plant pathology
laboratory remains a persistent problem. It can lead to losses ranging
from very minimal to the catastrophic loss of whole day work or even
the whole set of experiment.