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The Cell-Ag World 2020.02
Cell-based meat (cultured meat)
Muscle cells Bioreactor
Culture
medium
Processing
Myoblast grown
at 200t scale
Looks like a
beer factory?
Future: Meat brewery
Steak brewery
Single use
culture bags⇒
Steak grows inside⇒
Ready to ship⇒
Seafood by cellular agriculture
Cell culture at
room temperature
Small ponds as cell source
Culture media
produced by
microalgae
Short food miles
Farm high-rise
Cell-ag
towers
-95% water use,
-98% land use?
Tuomisto 2011, Oxford
Farmscape, A.D.2203
Vertical farms
Vast land
reverted to
nature
Artificial photosynthesis
Cellular agriculture manu-
facturing industry complex
Martian food production facility
The technical problem
$325,000
Cell-based burger, 200g
Technological milestones
Scaling & automation
Inexpensive media
Openness & public trust
Appealing products
Cell-Ag movement and spin-offs
Citizen Science
For-profit Startup
Non-Profit
2014 2015 2017 2019
“Open Source Cultured Meat”
Growing meat at home
Schoolkids culture
cells in kitchens
Manuals in Comiket
Airtight box
CO2 source humidity source
dish
“Food grade” demo - Sports drink culture media
90% DAKARA
80%
70%
60%
0%
(DMEM only)
50%
DaysCelldivisions
Proliferation of mouse L6 in DMEM/GreenDakara 10% FBS
#pH and osmolality of Dakara adjusted by NaHCO3(s) and 2M NaOH
Fluid name osmolality pH
DMEM(-, hi glu) 345 7.4
Pocari 338 3.4
Aquarius 291 3.37
Amino-Value 4000 289 3.63
AminoVital Gold 186 3.33
Vitamin Water 302 3.3
Green DAKARA 322 3.28
Amiiru Water 249 3.4
Mamoru Chikara 546 3.58
“CulNet System” proof of concept

▲PoC #1 and #2   ▼Automated PoC#3 2018~
2016 2017
Cultured avian
liver paste
Cultured
muscle tissue
R&D roadmap

Functional
compound
s, i.e.
cosmetics
Pasty foods,
i.e. foie gras
Processed
meat
salami
Food
Medium
Serum BloodHormone
Vascular
ization
2020 2021 2023
Tissues,
i.e. steak
2025
R&D
JP Pat. filed
2018-210910

“Space Salt”
end of 2019
2019
The road ahead in protein transformation
Meat by locals,
democratization
Hype cycle
Price parity reached
...by categories & regions
Meat from complexes
2020~ 2030~Animal meat
Cell-based
meat
Price
Time
Foie gras Beef Chicken
Importer Half-producer Producer
Less cows
Bioreactors in
the backyard
Many cows
Cows are the product Cells or meat is the product
Traditional farmscape Cell-ag farmscape
The Cell-Ag Farmscape
Less feeding, cleaning and waste treatment
More facility management as in beer brewery
“Star Cow Talent Agency”
・Brands by
individuals, not
breed lines
・Animal talent
agencies
Talent agency
Cell-based meat
Brand
development
Food
companies
Cost of DNA synthesis Cost of cell culture
“Write DNA & grow cells”
DIY insulin for those
who can’t afford
http://openinsulin.org/
Concept demonstrated
DIY cell culture
Pharma
company DIY gene edit
Cheap DNA
DIY cell culture,
manufacture
drugs at home
”Drug DNA leaked to 4chan”
Capybara Electric eel Yellow fish
Design an
animal like
LEGO
Designed animal &
its meat from
home culture vat
A fictional electric rat for pet
Prototyping of alien biospheres
Avatar (2009),
Forest of Pandora
Perhaps, someday in the future on Mars….

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EmTechAsia 2020 (postponed) Cell-based meat

  • 2. Cell-based meat (cultured meat) Muscle cells Bioreactor Culture medium Processing
  • 3. Myoblast grown at 200t scale Looks like a beer factory? Future: Meat brewery
  • 4. Steak brewery Single use culture bags⇒ Steak grows inside⇒ Ready to ship⇒
  • 5. Seafood by cellular agriculture Cell culture at room temperature Small ponds as cell source
  • 6. Culture media produced by microalgae Short food miles Farm high-rise Cell-ag towers -95% water use, -98% land use? Tuomisto 2011, Oxford
  • 7. Farmscape, A.D.2203 Vertical farms Vast land reverted to nature Artificial photosynthesis Cellular agriculture manu- facturing industry complex
  • 10. Technological milestones Scaling & automation Inexpensive media Openness & public trust Appealing products
  • 11. Cell-Ag movement and spin-offs Citizen Science For-profit Startup Non-Profit 2014 2015 2017 2019
  • 12. “Open Source Cultured Meat” Growing meat at home Schoolkids culture cells in kitchens Manuals in Comiket Airtight box CO2 source humidity source dish
  • 13. “Food grade” demo - Sports drink culture media 90% DAKARA 80% 70% 60% 0% (DMEM only) 50% DaysCelldivisions Proliferation of mouse L6 in DMEM/GreenDakara 10% FBS #pH and osmolality of Dakara adjusted by NaHCO3(s) and 2M NaOH Fluid name osmolality pH DMEM(-, hi glu) 345 7.4 Pocari 338 3.4 Aquarius 291 3.37 Amino-Value 4000 289 3.63 AminoVital Gold 186 3.33 Vitamin Water 302 3.3 Green DAKARA 322 3.28 Amiiru Water 249 3.4 Mamoru Chikara 546 3.58
  • 14. “CulNet System” proof of concept
 ▲PoC #1 and #2   ▼Automated PoC#3 2018~ 2016 2017 Cultured avian liver paste Cultured muscle tissue
  • 15. R&D roadmap
 Functional compound s, i.e. cosmetics Pasty foods, i.e. foie gras Processed meat salami Food Medium Serum BloodHormone Vascular ization 2020 2021 2023 Tissues, i.e. steak 2025 R&D JP Pat. filed 2018-210910
 “Space Salt” end of 2019 2019
  • 16. The road ahead in protein transformation Meat by locals, democratization Hype cycle Price parity reached ...by categories & regions Meat from complexes 2020~ 2030~Animal meat Cell-based meat Price Time Foie gras Beef Chicken Importer Half-producer Producer
  • 17. Less cows Bioreactors in the backyard Many cows Cows are the product Cells or meat is the product Traditional farmscape Cell-ag farmscape The Cell-Ag Farmscape Less feeding, cleaning and waste treatment More facility management as in beer brewery
  • 18. “Star Cow Talent Agency” ・Brands by individuals, not breed lines ・Animal talent agencies Talent agency Cell-based meat Brand development Food companies
  • 19. Cost of DNA synthesis Cost of cell culture “Write DNA & grow cells” DIY insulin for those who can’t afford http://openinsulin.org/ Concept demonstrated DIY cell culture
  • 20. Pharma company DIY gene edit Cheap DNA DIY cell culture, manufacture drugs at home ”Drug DNA leaked to 4chan”
  • 21. Capybara Electric eel Yellow fish Design an animal like LEGO Designed animal & its meat from home culture vat A fictional electric rat for pet
  • 22. Prototyping of alien biospheres Avatar (2009), Forest of Pandora
  • 23. Perhaps, someday in the future on Mars….