Phenotyping common bean populations and breeding  lines for drought and BSM tolerance in Ethiopia   Asrat AA, Fistum A, Da...
Act. 3: Deploy insect and disease resistance genesMilestone 5: QTL for bean stemmaggot resistance• Two populations evaluat...
Act. 3: Deploy insect and disease resistance genesMethodology• The experiments were conducted at three locations      • Ar...
Act. 3: Deploy insect and disease resistance genes Methodology  The materials evaluated in three groups       14 regiona...
Act. 3: Deploy insect and disease resistance genes    Data collected•   All phenology data were collected•   BSM count per...
Areka non-stress                     Humbo non-stress                    Humbo stressAreka stress
G2333 x G19839 RIL                                           with adventitious root                                       ...
BAT881                                                                            BAT881 x G21212 RIL                     ...
Grain yield kgha-1 Stress                                     Grain yield kgha-1 NS                    Performance of RILs...
Act. 4: Deploy drought tolerance traits in                   Andean gene poolMilestone 6: 36 Andean genotypes characterize...
Act. 4: Deploy drought tolerance traits in                    Andean gene poolMilestone 7: Three visual scales for drought...
Act. 4: Deploy drought tolerance traits in                  Andean gene poolMilestone 11: 1 population (drought-tolerant D...
Marker -assisted recurrent selection scheme on beans                                     Single and multiple cross        ...
Cycle two                 Selection of the best lines                                                               Genoty...
Description of phenoytping Locations• Arbaminch  – is located in the Gamo Gofa Zone of the Southern Nations,    Nationalit...
MARS population early growth
MARS population evaluated byProfessors from University of Free       State and Dr Beebe
Support from GCP• Irrigation system
Support from GCP• Technician training on farm machinery
Acknowledgments•   GCP•   CIAT•   Dr Steve Beebe•   Dr Hannibal M from AgriconInternational•   Jake Bentum•   Dr Bodo•   G...
TLI 2012: Bean breeding - Ethiopia
TLI 2012: Bean breeding - Ethiopia
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TLI 2012: Bean breeding - Ethiopia

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TLI 2012: Bean breeding - Ethiopia

  1. 1. Phenotyping common bean populations and breeding lines for drought and BSM tolerance in Ethiopia Asrat AA, Fistum A, Daniel A, Yayis R SARI- Ethiopia
  2. 2. Act. 3: Deploy insect and disease resistance genesMilestone 5: QTL for bean stemmaggot resistance• Two populations evaluated for BSMand drought combined stress in Ethiopiaat three locations as part of M.Sc. Objectives:Thesis • To estimate the phenotypic and genotypic• 14 released varieties evaluated for variability for Morpho-physiologicalBSM and drought combined stress traits and asses the genetic variability fortolerance in Ethiopia at three locations drought and BSM resistance among common bean recombinant lines • To evaluate the performance of the recombinant lines of two populations from crosses G2333 x G19839 and BAT881 x G21212 for their drought and BSM combined resistance. • To evaluate the contribution of adventitious roots for BSM resistance
  3. 3. Act. 3: Deploy insect and disease resistance genesMethodology• The experiments were conducted at three locations • Areka • Humbo • Wondogenet• Where BSM infestation is very common• Four managed environments were created – 1. Non stress • Chemical treated for BSM and fully irrigated – 2.Intermittent Drought Stressed (Humbo and Wondogenet) • Stressed before flower initiation and at pod initiation – 3. Terminal drought stress at flower initiation (Humbo and Wondogenet) – 4. Terminal drought stress at pod initiation (Areka, Humbo and Wondogenet)
  4. 4. Act. 3: Deploy insect and disease resistance genes Methodology  The materials evaluated in three groups 14 regionally and nationally released common bean varieties 82 RILs from cross G2333 x G19839 ( CIAT) 100 RILS from cross BAT881 x G21212 ( CIAT) using 14*14 simple lattice design Two rows plot leaving one row between plots 60 cm spacing between rows and 10 cm between plants spacing
  5. 5. Act. 3: Deploy insect and disease resistance genes Data collected• All phenology data were collected• BSM count per plant• % of lodged plants due to BSM• BSM damage score per plot• BSM damage score per Individual plant• SPAD reading• Canopy temp• Root pulling resistance• Stem Diameter• PHI• Yield• Pod wall thickness
  6. 6. Areka non-stress Humbo non-stress Humbo stressAreka stress
  7. 7. G2333 x G19839 RIL with adventitious root BAT881 x G21212 RILField performance of two RIL populationsunder drought and BSM stress
  8. 8. BAT881 BAT881 x G21212 RIL G19839 G21212Grain yield kgha-1 Stress G2333 Regional varieties G19839 x G2333 RIL Grain yield kgha-1 NS Performance of RILs, parents and regional varieties for combined effect of DS & BSM at Areka, Ethiopia 2011
  9. 9. Grain yield kgha-1 Stress Grain yield kgha-1 NS Performance of RILs, parents and regional varieties for combined effect of DS & BSM at Humbo, Ethiopia 2011
  10. 10. Act. 4: Deploy drought tolerance traits in Andean gene poolMilestone 6: 36 Andean genotypes characterized for drought traits• 36 Andean genotypes were evaluate at Awassa Ethiopia under irrigated and stressed conditions and data on canopy temperature, root pulling resistance, PHI and grain yield
  11. 11. Act. 4: Deploy drought tolerance traits in Andean gene poolMilestone 7: Three visual scales for drought• 64 lines developed from non-wilting parentswere evaluated at Awassa Ethiopia for wiltingresistance, root pulling resistance and PHI
  12. 12. Act. 4: Deploy drought tolerance traits in Andean gene poolMilestone 11: 1 population (drought-tolerant Durango x Andean) of 190 lines phenotyped for MARS (years 2 and 3), in Colombia and Ethiopia – 126 DAB lines evaluated in Ethiopia at two sites under drought stress and non-stress environments using 9 x 14 alpha lattice design – 72 SAB lines evaluated in Ethiopia at two sites under drought stress and non-stress environments using 9 x 8 alpha lattice• Population development and schematic diagram of MARS• Population developed from single and multiple crosses • Cross 16357 (CAL 143 x SAB 620) x SAB 626 • Cross 16358 (SAB 628 x CAL 143) x SAB 659 • Cross 16359 (SAB 628 x CAL 143) x SAB 686• Advanced to F3-6 generation
  13. 13. Marker -assisted recurrent selection scheme on beans Single and multiple cross Cross 16357 (CAL 143 x SAB 620) x SAB 626 Cross 16358 (SAB 628 x CAL 143) x SAB 659Cycle one Cross 16359 (SAB 628 x CAL 143) x SAB 686 DAB lines F1 F2 Genotyping of F3-6 Pheotyping of the whole parents (CIAT) population(Colombia and Ethiopia) Genotyping of the whole population (CIAT) QTL
  14. 14. Cycle two Selection of the best lines Genotyping of the based on QTL results selected genotypes Intercross the best lines (at least 10 genotypes/family ) F2 Pheotyping of the Genotyping of the population population (Colombia and Ethiopia) (CIAT) QTL Development of the best lines
  15. 15. Description of phenoytping Locations• Arbaminch – is located in the Gamo Gofa Zone of the Southern Nations, Nationalities, and Peoples Region about 500 kilometres south of Addis Ababa, at an elevation of 1285 meters above sea level. – Geographical coordinates: 6° 2 0" North, 37° 33 0" East. – The soil is characterized in clay loam texture.• Wondo genete – is located in the Sidama Zone of the Southern Nations, Nationalities, and Peoples Region about 300 kilometres South of Addis Ababa, – at an elevation of 1760 meters above sea level. – The Geographical coordinates: ?? – The soil is characterized in loam texture.
  16. 16. MARS population early growth
  17. 17. MARS population evaluated byProfessors from University of Free State and Dr Beebe
  18. 18. Support from GCP• Irrigation system
  19. 19. Support from GCP• Technician training on farm machinery
  20. 20. Acknowledgments• GCP• CIAT• Dr Steve Beebe• Dr Hannibal M from AgriconInternational• Jake Bentum• Dr Bodo• Gillian Summers• SARI

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