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GRM 2011: Improving drought tolerance in chickpeas for Africa and Asia


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GRM 2011: Improving drought tolerance in chickpeas for Africa and Asia

  1. 1. Improving drought tolerance in chickpeafor Africa and AsiaPooran GaurProject Delivery CoordinatorICRISAT, Patancheru, Indiaon behalf of Chickpea GroupGCP Challenge Initiative
  2. 2. Target countries for chickpea CIIndiaEthiopiaKenyaTanzania
  3. 3. Various projects linked to chickpea CIG6007.04/G6010.04: Tropical Legumes I (TL I) - Objective 4: ChickpeaPhase 1&2 (2007- 2014)PI and Lead Institute: Rajeev Varshney, ICRISATG4008.12: Linking genetic diversity with phenotype for drought tolerancein reference collection (2008 - 2011)PI and Lead Institute: L Krishnamurthy, ICRISATG7009.02: Mapping and validation of QTL for drought tolerance traits(2009 -2011)PI and Lead Institute: Pooran Gaur, ICRISATG7009.06: Development of a SNP platform (Nov 2009-Oct 2011)PI and Lead Institute: Doug Cook, UC-DavisTL-I complementary project in India - funded by The Dept. ofBiotechnology, Govt. of India (2011-14)PI and Lead Institute: Rajeev Varshney, ICRISAT
  4. 4. Major Activities1. Development of genetic and genomicresources2. Identification and validation of markersassociated with QTL for drought tolerancetraits3. Marker-assisted introgression of droughttolerance traits in farmer-preferred cultivars4. Marker-assisted recurrent selection (MARS)for cumulating favorable alleles for droughttolerance
  5. 5. • 1655 novel SSR markersdeveloped and validated. Withthese the total number of SSRmarkers available for chickpeais over 2500• GoldenGate assay for 768 SNPsdeveloped• 2486 KASPar SNPs weredesigned. 1484 SNPs werefound polymorphic in a set of94 genotypes.Development of molecularmarkers for chickpeaHomozygoteHeterozygoteHomozygoteNTC
  6. 6. Development of high density linkage mapchickpea1291 loci were mapped on to eight linkage groupsThe total map distance ~845.5 cMAverage inter marker distance 0.6 cM/marker
  7. 7. Development of MAGIC linesNo. of crosses28 2-way14 4-way7 8-way>1000 F2 plants from 8-way crosses are currently being grown in a greenhouse(A) ICC 4958 (B) ICCV 10 (C) JAKI 9218 (D) JG 11(E) JG 130 (F) JG 16 (G) ICCV 97105 (H) ICCV 001088 Parents used in development of MAGIC lines
  8. 8. GA24STMS11TAA170N121GA24TAA170N142Identified and validation of drought QTLICC 283 × ICC 8261ICC 4958 × ICC 1882QTL forRoot traitsHarvest IndexYield related traitsΔ13C
  9. 9. Cross: Cultivars x Donors for root traits(Confirm hybrids)BC1: Cultivar x F1↓BC1F1(Foreground and background selection)BC 2: Cultivar x BC1F1↓BC2F1(Foreground and background selection)BC3: Cultivar x BC2F1↓BC3F1(Foreground and background selection)↓BC3F2(Selected homozygous plants for QTL-linked markers)↓2010/11 BC3F3Seed multiplication and preliminary evaluation↓2011/12 Multilocation evaluation of BC3F4 linesMABC for improving drought toleranceDonorsCultivarsJG 11Chefe KAK 2
  10. 10. Evaluation of MABC linesfor root traitsVertical bars denote standard error of differences. The means were significantly different at 0.001 level and werebased on 8 replicated cylinders with 2 plants in each cylinder)Donor line Elite lineDonor line Elite lineMABC linesMABC lines
  11. 11. MABC being run by NARS in TL-I Phase IIEgerton University - KenyaICCV 95423 × ICC 4958ICCV 97105 × ICC 4958DZARC - EthiopiaEjere × ICC 4958Areti × ICC 4958IIPR-KanpurDCP 92-3 ×ICC 4958IARI-New DelhiPusa 362×ICC 4958
  12. 12. MARS for improving drought tolerance2 Crosses2008/09 Genotyping of F3 plants2009/10 F4 (Seed mutiplication)2010/11 Multilocation evaluation(Ethiopia, Kenya, India) of F5progenies and QTL analysis2011/12 Selection and inter-crossingof selected lines•Two good-by-good crosses involving cultivars fromAfrica and Asia28 new crosses available for initiating MARS
  13. 13.  Workshop on “Modern Breeding Technologies for ChickpeaImprovement” held during Oct 25 – Nov 19, 2010 16 scientists participated12 from five countries ofAfrica (Ethiopia, Kenya,Tanzania, Malawi,Algeria)and 4 from fourcountries of Asia (India,Nepal, Bangladesh,Myanmar) Included lectures, demo, field/lab visits, comprehensive data analysis,interaction with different subject matter specialists at ICRISATCapacity building:Modern breeding workshop
  14. 14.  Kenya:- Ms Serah Songok, PhD student, Egerton University- Ms Alice Koskie, PhD student, WACCI- Mr Moses Oyier, MSc student, Egerton University Ethiopia:- Mr Musa Jarso, PhD student, Addis Ababa University- Mr Kebede Teshome, PhD student, Haramaya Uni.- Mr Getachew Tilahun, Addis Ababa UniversityCapacity Building:Degree students
  15. 15. Field trial and data management• 11 datasets compiled and curated• Trait ontology made available• Pedigree information of ICRISAT’s chickpea breedingprogram moved to ICIS• Plan to use IB Fieldbook from coming crop season• Handheld evaluated and keen to use Galaxy tab 10.1
  16. 16. Linkages with TL-II• Key partners are common• Joint capacity building onintegrated breeding ofchickpea• Products from TL-I alreadybeing integrated in TL-IIactivities
  17. 17. The Chickpea CoP was established at the TL-I annualmeeting in Madrid during May 2011.CoP Coordinator: Pooran Gaur, ICRISATCoP Mentor: Teresa Milan, Universidad de Cordoba, SpainAim: to promote knowledge sharing and mutual learningon integrated chickpea breedingCommunity of Practice (CoP) forintegrated breeding of chickpea
  18. 18. Partners in Chickpea CI• ICRISAT- Patancheru, India (Pooran Gaur, Rajeev Varshney, LKrisnamurthy, Vincent Vadez, Hari Upadhyaya, Trushar Shah, CSivakumar, M Thudi)- Nairobi, Kenya (NVPR Ganga Rao)• Egerton University, Kenya: (Paul Kimurto)• EIAR, Ethiopia (Asnake Fikre)• LZARDI, Tanzania (Robert Kileo)• UAS-Bangalore, India (KP Vishwanatha, MS Sheshashaye)• ANGRAU-Hyderabad, India (Veera Jayalakshmi)• IIPR-Kanpur, India (SK Chaturvedi, Aditya Garg)• IARI-New Delhi (Shailesh Tripathi, Ch Bharadwaj, J Kumar)• RSKVV-Gwalior, India (M Yasin)• RAU-Bikaner, India (SJ Singh)• UC−Davis, USA (Doug Cook)• University of Frankfurt, Germany (Peter Winter, Guenter Kahl)• JIRCAS, Japan (Satoshi Tobita, Osamu Ito)• DArT P/L (Andrzej Killian)