Cotton improvement programmes primarily lay emphasis on development of hybrids, which have contributed in improving the productivity of cotton.
Many population improvement schemes are followed in cross pollinated crops to increase genetic diversity, to create heterotic groups and exploit them.
There is a steep rise in production of long staple cotton, while production of extra long staple, short staple and even medium staple cotton has come down drastically. The success of Interspecific hybrids has lead to overcome the acute shortage of ELS cotton which the country was experiencing during 1970s.
Realizing the need for developing potential interspecific (H×B) hybrids, a details study was initiated at UAS Dharwad during 2007/08 to identify Hirsutum and Barbadense genotypes capable of giving potential interspecific hybrids.
Molecular markers increasingly detect locus differences among genotypes and represent a powerful tool for the assessement of genetic diversity in plant species
Interspecific hybrids are known to be more susceptible to biotic stress. It is hence important to develop Bt version for interspecific hybrid.
Study of genetic variability in germplasm of common bread wheatYANKEY BHUTIA
Similar to Recombinational variability for combining ability among F4 barbadense lines, molecular marker and genetic transformation study in cotton (20)
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Recombinational variability for combining ability among F4 barbadense lines, molecular marker and genetic transformation study in cotton
1.
2. Recombinational variability for combining ability among
F4 barbadense lines, molecular marker and genetic
transformation study in cotton
Chairperson
Dr .S.S.PATIL
Yanal Alkuddsi
PGS 09AGR 4949
Department of Genetics & plant
Breeding
4. • Cotton improvement programmes primarily lay emphasis on development of
hybrids, which have contributed in improving the productivity of cotton.
• Many population improvement schemes are followed in cross pollinated
crops to increase genetic diversity, to create heterotic groups and exploit
them.
• There is a steep rise in production of long staple cotton, while production of
extra long staple, short staple and even medium staple cotton has come
down drastically. The success of Interspecific hybrids has lead to overcome
the acute shortage of ELS cotton which the country was experiencing during
1970s.
• Realizing the need for developing potential interspecific (H×B) hybrids, a
details study was initiated at UAS Dharwad during 2007/08 to identify
Hirsutum and Barbadense genotypes capable of giving potential interspecific
hybrids.
• Molecular markers increasingly detect locus differences among genotypes
and represent a powerful tool for the assessement of genetic diversity in
plant species
• Interspecific hybrids are known to be more susceptible to biotic stress. It is
hence important to develop Bt version for interspecific hybrid.
5. 0
50
100
150
200
250
300
350
400
03-04. 04-05. 05-06. 06-07. 07-08. 08-09. 09-10. 10-11. 11-12.
Quantityinlakhbalesof170kgs
Year
Staple-wise production of cotton in the Bt Cotton Era
Short (Below 20.0 mm) Medium/Medium Long (20.5 to 27 mm) Long Staple (27.5 to 32.0 mm) Extra Long staple (32.5 mm & above) TOTAL
The lopsided Change in Composition of Fibre classes in Cotton
•At the time of Independence India was mostly producing Short staple cotton
•Hirsutum Cottons brought boost in Medium staple cotton production
•Interspecific Hybrids brought self sufficiency and even export of ELS cotton
•With the onset of Bt cotton era, there is an Increase in total cotton production and
Long Staple cotton production but a relative reduction in Other classes
Declaresing Diversity : from Fibre quality angle What is the impact of this?
6. • Development of Hirsutum vs Barbadense heterotic groups.
• Exploiting heterotic groups by creation of recombinational variability in
G.barbadense F4 population for ability to combine with selected diverse G.
hirsutum testers.
• To determine combining ability effects (gca and sca), variances (GCA and
SCA), combining ability patterns of these Barbadense lines.
• Identifying suitable Barbadense lines for developing heterotic sub populations
against Hirsutum testers.
• Determining correlations, direct and indirect effects of component characters
on seed cotton yield.
• Evaluation of genetic diversity in G. barbadense cotton lines and G. hirsutum
testers based on SSR molecular markers.
• Relationship between molecular diversity and hybrid performance in
interspecific hybrids of cotton.
• Standarizing `in planta Agrobacterium tumefacience mediated genetic
transformation protocol to develop new events by transforming G. hirsutum
based on Cry1Ac-Cry1Ec genes .
7. 1. Estimation of heterosis
2. Combining ability effects and variance
3. Development of heterotic groups and exploiting them
4. Studies of correlation and path coefficient analysis
5. Molecular marker study in cotton
6. In planta genetic transformation
8. 1. Development of heterotic groups
1.1 Line x Tester for confirmation of interspecific heterotic groups
In this experiment, 92 (YHB trial) interspecific hybrids (G.hirsutum x
G.barbadense) along with three checks (RAHB 87, MRC 6918 and
DCH 32) subjected to Line x Tester analysis (23 hirsutum testers and
4 barbadense lines), was laid out in Randomized Block Design (RBD)
with two replications. Each entry was sown in 2 row plots spaced at
90 cm with recommended dose of fertilizer and seeds were sown on
28-6-2010, 2-3 seeds were dibbled per spot in each row and
thinning was attended to retain one healthy plant per hill at 25 days
after sowing. All the recommended package of practices were
followed to rise healthy crop.
9. 2.1 Mean per se performance of best HB interspecific hybrids
For mean performance of 49 (best HB trial)
hybrids, the hybrids along with three checks
(RAHB 87, MRC 6918 and DCH 32) was laid
out in Randomized Block Design with two
replications. Each hybrid was sown in 4
rows plots with spacing of 90 cm x 60 cm
with recommended dose of fertilizer and
the trial was sown on 27/6/2010 .
10. 2. Evaluation of recombinational variability for combining ability
2.1 Choice of the material
Fig 1. Schematic representation of research activities
11. Sl. No. Cotton / Entry
2.5% SL
(mm)
% Micronaire value Maturity Ratio Tenacity (g/t) Elongation %
1 (DB 533 x DB 534 F3 IPS 62) 33.53 44.80 3.11 0.62 30.03 6.03
2 (DB 533 x DB 534 F3 IPS 49) 33.5 45 3.1 0.62 30.0 6.0
3 (DB 533 x DB 534 F3 IPS 23) 35.7 47 3.2 0.63 29.8 5.6
4 (DB 533 x DB 534 F3 IPS 36) 35.8 48 3.5 0.67 29.4 5.6
5 (DB 533 x DB 534 F3 IPS 15) 36.7 47 3.0 0.59 29.3 5.5
6 (DB 533 x DB 534 F3 IPS 1) 37.7 46 3.0 0.59 28.5 5.8
7 (DB 533 x DB 534 F3 IPS 33) 37.4 47 3.2 0.63 28.4 5.4
8 (DB 533 x DB 534 F3 IPS 24) 36.8 46 3.2 0.62 28.3 5.6
9 (DB 533 x DB 534 F3 IPS 16) 35.4 48 3.3 0.63 28.3 5.6
10 (DB 533 x DB 534 F3 IPS 52) 33.2 47 3.9 0.69 28.3 6.1
11 (DB 533 x DB 534 F3 IPS 12) 37.1 46 3.2 0.63 28.3 5.3
12 (DB 534 x DB 533 F3 IPS 22) 32.2 47 3.2 0.65 28.2 6.0
13 (DB 533 x DB 534 F3 IPS 14) 36.9 48 3.4 0.65 28.2 5.4
14 (DB 533 x DB 534 F3 IPS 34) 38.2 46 3.2 0.62 28.2 5.5
15 (DB 533 x DB 534 F3 IPS 55) 35.7 46 3.5 0.65 28.0 5.4
16 (DB 533 x DB 534 F3 IPS 17) 32.1 46 3.0 0.64 27.8 6.1
17 (DB 533 x DB 534 F3 IPS 32) 38.4 45 3.1 0.62 27.8 5.4
18 (DB 533 x DB 534 F3 IPS 38) 38.4 47 3.0 0.61 27.8 5.4
19 (DB 533 x DB 534 F3 IPS 13) 32.6 47 3.3 0.66 27.7 5.9
20 (DB 533 x DB 534 F3 IPS 48) 31.4 45 3.7 0.69 27.7 6.0
21 (DB 533 x DB 534 F3 IPS 6) 33.0 46 3.7 0.67 27.6 5.8
22 (DB 533 x DB 534 F3 IPS 8) 37.1 43 3.1 0.61 27.6 5.6
23 (DB 533 x DB 534 F3 IPS 44) 32.37 48.40 3.99 0.70 27.40 6.07
24 (DB 533 x DB 534 F3 IPS 71) 39.37 47.70 3.25 0.64 25.97 5.93
25 (DB 533 x DB 534 F3 IPS 105) 34.60 45.83 3.80 0.69 25.67 6.00
26 (DB 533 x DB 534 F3 IPS 26) 36.97 46.40 3.55 0.67 25.53 6.00
27 (DB 533 x DB 534 F3 IPS 30) 37.77 47.87 3.32 0.63 25.50 5.87
28 (DB 533 x DB 534 F3 IPS 25) 31.77 47.20 3.61 0.66 25.13 6.13
Table 1. List of F3 barbadense parents involved in the study of crossing and recombinational variability
12. 2.2 Crossing Programme
2.3Evaluation of Derived F1s and F5 barbadense lines
2.4 Collection of data and recording of observations
Plant height (cm) Number of monopodia per plant Number of sympodia per plant
Number of bolls per plant Mean boll weight (g) Reproductive points on sympodia
Sympodial length at 50 per cent plant height (cm) Inter branch distance (cm)
Seed index (g) Ginning outturn (%) Lint index (g) Seed cotton yield (kg ha-1)
Photosynthetic rate (μmolCO2m-2s-1) Stomatal conductance (μmolm-2s-1)
Transpiration rate (mmol H2Om-2s-1) 2.5% span length (mm)
Fiber uniformity ratio (%)
Fiber micronaire value (g/inch)
Fiber maturity ratio (%)
Fiber strength (g/tex) Fiber elongation (%)
13. Plate 4. Recording the physiological parameters by using IRGA instrument
14. 2.5 Statistical Analysis
2.5.1 Combining ability analysis
2.5.1.1 Analysis of variance for combining ability Kempthorne (1957)
2.5.1.2 ANOVA table outlines by Panse and Sukhatme (1961)
2.5.1.3 Line Tester analysis
2.5.1.4 Estimation of heterosis Turner (1953) and Hayes et al. (1955)
2.5.1.5 Test of significant for heterosis
2.5.1.6 Efficiency of tester Patil (1995)
2.5.1.7 Combining ability analysis Kempthorne (1957)
2.5.1.8 Estimates of variances
2.5.1.9 Estimate of combining ability effects
2.5.1.10 Overall gca status of parents, sca status and heterosis of crosses Mohan
Rao (2001)
2.5.1.11 Pooled score for gca effects Arunachalam and Bandopadhyay (1979)
2.5.1.12 Standard errors of the effects
2.5.1.13 Characterization of combining ability pattern (status) of F4 lines Patil (1995)
2.5.1.14 Test of significance for genotypic differences
2.5.1.15 Variance
2.5.1.16 Coefficient of variance (CV)
2.5.1.17 Correlation coefficient analysis Weber and Moorthy (1952)
2.5.1.18 Path coefficient analysis Wright (1921)
2.5.1.19 Intergeneration (F3-F5) correlation and regression analysis
2.5.1.20 Narrow sense heritability estimates Cahaner and Hillet (1980)
15. • The experimental results obtained in the
present study are presented under the following
heads.
• Development of heterotic groups (hirsutum vs
barbadense) based on combining ability and
hybrid performance
• Evaluation of recombinational variability for
combining ability
• Molecular marker study in cotton
• In planta genetic transformation
16. Development of heterotic groups (hirsutum vs barbadense) based
on combining ability and hybrid performance
1. Line x Tester analysis (YHB hybrids) for confirmation of
interspecific heterotic groups
17. Table 3. Mean sum of squares for eight characters (RBD) in interspecific YHB crosses
Source d.f.
Mean sum of squares
Seed cotton
yield (kg/ha)
Plant
height
(cm)
No. of
sympodia
No. of bolls
Mean
boll
weight
(g)
Seed
index (g)
Ginning
outturn
(%)
Lint
index (g)
1 2 3 4 5 6 7 8
Replications 1 1464781.85** 1722.35 198.14** 94.58** 2.29** 37.90** 12.28 1.85*
Crosses 91 365118.66** 722.32 13.58 43.03** 0.23** 2.56** 13.51** 0.95**
Error 91 47434.74 831.52 12.26 1.94 0.04 0.74 3.45 0.36
S.E (d) - 159.37 20.26 2.45 1.93 0.45 0.62 1.30 0.42
CD at 5% - 447.51 57.10 6.93 5.42 0.89 1.74 3.65 1.18
CD at 1% - 592.58 75.84 9.21 7.18 1.18 2.31 4.83 1.57
* Significant at P = 0.05 ** Significant at P = 0.01
18. Sl. No. Crosses
Seed
cotton
yield
(kg/ha)
Plant
height (cm)
No. of
monopodi
a
No. of
sympodia
No. of
bolls
Mean boll
weight (g)
Reprodu
ction
Points on
Sympodia
Length of
sympodia
at 50%
height
Inter
Branch
Distant
(cm)
Seed
index
(g)
Ginning
outturn
(%)
Lint
index
(g)
2.5%
SL
(mm)
%
Micronaire
value
(µg/inch)
Maturity
Ratio (%)
Tenacity
(g/t)
Elonga
tion %
Value Rank BMC 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
1 DH 18-31 x DB 533 3128.7
2
1 BMC 263.84 2.00 28.84 24.20 4.60 6.33 64.92 32.83 12.19 27.66 4.73 37.30 45.20 3.11 0.61 24.83 6.57
2 DH 49-1 x DB 533 2615.4
0
9 242.34 2.00 27.84 32.92 4.20 6.09 62.09 28.84 11.33 24.10 3.62 33.63 44.47 2.83 0.59 25.83 6.67
3 DH 23-1 x DB 533 2283.36 24 239.50 2.50 32.84 29.84 3.98 8.67 69.42 31.17 11.12 29.59 4.69 35.10 44.90 3.24 0.63 26.39 6.53
4 DH 98-27 x DB 533 2525.4
2
11 BMC 251.34 2.50 32.50 39.75 3.45 4.83 64.17 28.67 13.00 29.72 5.48 38.53 45.00 3.31 0.65 23.45 6.50
5 178-24 x DB 533 2487.6
4
13 BMC 244.83 1.84 32.00 31.59 4.23 5.25 56.42 27.84 12.55 34.46 6.64 38.27 42.50 2.87 0.59 23.88 6.57
6 DH46-1 x DB 533 2808.0
3
4 241.83 2.50 28.17 35.00 3.95 5.17 58.50 30.17 11.95 22.87 3.60 36.17 43.67 2.88 0.59 25.80 6.73
7 ZCH 8 x DB 533 2868.2
3
3 BMC 275.17 1.84 28.50 30.67 4.08 6.67 66.67 35.50 10.01 31.30 4.54 35.43 44.43 3.44 0.65 23.91 6.73
8 DH 37-4 x DB 533 2641.4
2
7 250.17 1.84 26.17 27.00 3.44 6.00 65.34 36.00 11.23 32.30 5.34 34.47 44.83 3.08 0.61 25.99 6.77
9 DH 24-4 x DB 533 2226.85 29 251.84 2.17 27.17 28.25 3.63 6.42 67.25 31.84 11.01 28.74 4.47 33.50 44.60 3.15 0.61 24.18 6.70
10 DH 8-7 x DB 533 1597.88 71 257.67 1.84 24.34 26.67 3.57 6.67 69.00 33.84 10.45 28.27 4.14 33.67 46.57 3.18 0.62 26.13 6.90
11 DH 11-8 x DB 533 2083.37 41 259.84 1.84 27.17 29.17 3.72 5.75 65.84 30.50 13.96 27.27 5.21 40.20 42.97 2.39 0.56 24.66 6.20
12 DH 29-1 x DB 533 1657.66 68 263.34 1.84 31.33 36.50 3.85 6.50 73.25 29.34 12.00 25.85 4.21 32.93 45.03 2.55 0.57 28.92 6.67
13 DH 53-2 x DB 533 2477.78 15 248.50 2.00 31.17 28.42 4.03 5.67 60.50 29.50 12.11 29.85 5.13 35.40 41.33 2.95 0.60 24.22 6.50
14 DH 35-17 x DB 533 2105.27 39 272.67 2.00 26.50 37.17 3.48 5.92 64.84 30.67 12.84 25.30 4.39 36.47 44.70 3.02 0.61 25.34 6.53
15 DH 23-4 x DB 533 2252.76 26 206.84 2.17 22.33 21.59 3.28 6.75 54.42 27.67 13.00 25.43 4.46 38.73 44.10 3.15 0.63 22.68 6.73
16 DH 31-2 x DB 533 1659.60 67 224.34 2.00 26.34 22.92 3.10 5.83 65.67 29.34 12.95 29.15 5.37 34.77 43.83 2.90 0.60 24.70 6.63
17 DH 13-7 x DB 533 3047.6
2
2 257.67 1.84 27.34 40.25 3.54 7.59 71.33 31.83 12.17 30.73 5.44 31.90 41.70 3.26 0.62 23.57 6.57
18 DH 75- 23 x DB 533 1561.45 76 233.84 2.17 26.67 27.42 3.33 6.17 60.59 30.50 11.52 26.54 4.19 37.60 40.93 2.87 0.60 23.15 6.43
19 DH 91-1 x DB 533 2162.55 34 246.00 2.00 26.34 29.34 3.30 6.09 61.09 31.00 13.12 26.60 4.74 38.90 41.13 2.97 0.60 23.38 6.27
20 DH 45-23 x DB 533 2305.34 23 243.00 3.00 31.17 30.25 3.63 5.50 61.08 29.67 12.37 27.11 4.63 33.93 43.57 2.99 0.61 24.58 6.80
21 DH 14-2 x DB 533 1584.00 73 257.17 1.84 31.00 35.17 3.78 6.00 65.92 30.67 13.19 24.07 4.21 37.67 40.07 3.11 0.62 24.04 6.73
22 DH 82-3 x DB 533 2392.87 19 245.00 1.84 28.33 34.34 3.45 5.83 66.34 32.00 12.00 31.34 5.46 35.93 43.53 2.96 0.59 24.97 6.70
23 DH 23-21 x DB 533 1757.80 65 235.50 1.84 28.17 37.92 3.45 8.25 68.00 27.84 12.50 27.09 4.61 40.17 42.73 2.86 0.58 23.20 6.37
24 DH 18-31 x DB 532 2282.92 25 304.33 1.50 30.17 29.34 3.60 8.59 81.92 30.67 14.73 23.47 4.52 37.80 41.97 2.95 0.58 24.11 6.43
25 DH 49-1 x DB 532 2246.47 27 238.67 2.00 31.84 35.84 4.25 6.25 66.34 30.33 11.97 25.96 4.17 35.87 39.27 2.86 0.59 23.67 6.17
Table 4. Mean per se performance of crosses in L x T study (YHB) in respect of seed cotton yield and its attributing characters
22. Source
d.f.
Mean sum of squares
Seed cotton yield
(kg/ha)
Plant
height
(cm)
No. of
sympodi
a
No. of
bolls
Mean
boll
weight
(g)
Seed
index
(g)
Ginning
outturn
(%)
Lint
index
(g)
1 2 3 4 5 6 7 8
Replications 1 1464781.85** 1722.35 198.14** 94.58** 2.29** 37.90** 12.28 1.85*
Crosses 91 365118.66** 722.32 13.58 43.03** 0.23** 2.56** 13.51** 0.95**
Line Effect 3 1344213.43** 705.20 0.99 56.37 0.61* 1.94 7.76 0.63
Tester Effect 22 903079.19** 541.25 11.19 45.34 0.28 3.86* 20.30* 1.46*
Line x Tester
Effect
66 141294.17** 783.46 14.95 41.65** 0.20** 2.16** 11.51** 0.80**
Error 91 47434.74 831.52 12.26 1.94 0.04 0.74 3.45 0.36
Table 5. Combining ability analysis of variance of crosses in L x T study (YHB) in respect of
seed cotton yield and its attributing characters
* Significant at P = 0.05 ** Significant at P = 0.01
23. Sl. No.
1
Characters
Variance Due to
GCA
Variance Due to
SCA
GCA/SCA
Plant height (cm) 11.49800 6463.54050 0.00275
2 No. of sympodia 0.01625 123.35975 0.00000
3 No. of bolls 0.91925 343.62825 0.00275
4 Mean boll Weight (g) 0.01025 1.65775 0.00675
5 Seed index (g) 0.03150 17.79225 0.00175
6 Ginning outturn (%) 0.12650 94.93500 0.00100
7 Lint index (g) 0.01050 6.56300 0.00150
8 Seed cotton yield (kg/ha) 21916.52625 1165676.92500 0.01700
Table 6. Variance due to general and specific combining ability for seed cotton yield and its
attributing characters
25. Parents
Seed cotton
yield (kg/ha)
Plant
height
(cm)
No. of
sympodia
No. of
bolls
Mean
boll
weight
(g)
Seed
index (g)
Ginning
outturn
(%)
Lint
index
(g)
Value Rank 2 3 4 5 6 7 8
DH 75- 23 -557.05** 21 -21.358* -1.556 -2.63** 0.17* -1.047** 0.873 -0.21
DH 91-1 67.27 14 7.102 1.486 -2.38** -0.15* -0.192 -0.937 -0.297
DH 45-23 25.64 17 9.601 2.069 -1.23* 0.15* 1.169** -0.313 0.396
DH 14-2 -538.49** 20 4.769 0.611 0.93 -0.09 0.019 0.136 0.07
DH 82-3 142.20 13 -2.814 -0.349 -0.05 -0.28** -0.323 1.226 0.138
DH 23-21 -458.05** 18 1.643 1.028 5.18** -0.3** 0.513 1.447* 0.547*
SE (gi) 77.00 10.20 1.24 0.49 0.07 0.30 0.66 0.21
SEd (gi-gj) 108.90 14.42 1.75 0.70 0.10 0.43 0.93 0.30
CD at 5% 215.62 20.25 2.46 1.38 0.21 0.61 1.30 0.42
CD at 1% 286.40 26.82 3.26 1.83 0.27 0.80 1.73 0.56
Barbadense
Lines
DB 533 242.92** 1 BMC 1.748 -0.118 -1.51** 0.00 -0.236 0.24 -0.027
DB 532 -4.92 3 1.146 0.194 0.52* -0.08* -0.014 -0.613* -0.14
DB 531 -313.54** 4 2.879 0.034 -0.07 -0.09** -0.015 0.15 0.025
DB 534 75.54* 2 BMC -5.774 -0.111 1.06** 0.16** 0.265* 0.23 0.142
SE (gi) 32.11 4.25 0.52 0.21 0.03 0.13 0.27 0.09
SEd (gi-gj) 45.41 6.01 0.73 0.29 0.04 0.18 0.39 0.13
CD at 5% 89.92 8.45 1.03 0.58 0.09 0.25 0.54 0.18
CD at 1% 119.44 11.19 1.36 0.76 1.83 0.33 0.72 0.23
* Significant at P = 0.05 ** Significant at P = 0.01 BMC: Bench Mark Crosses
26. Testers gca effects
Lines gca effects
Fig 5. General combining ability effects for lines and testers in respect of seed cotton yield (kg/ha) in YHB crosses
27. Crosses
Seed
cotton
yield
(kg/ha)
Plant height
(cm)
No. of
sympodia
No. of bolls
Mean boll
weight (g)
Seed
index(g)
Ginning
outturn)%(
Lint index
(g)
1 2 3 4 5 6 7 8
DH 18-31 x DB 533 528.15** 11.504 2.12 -3.7** 0.65** -0.93 0.532 -0.15
DH 49-1 x DB 533 179.83 -2.121 -0.757 0.86 0.16 -0.712 -1.797 -0.603
DH 23-1 x DB 533 -20.16 -6.165 3.245 -1.78 0.24 -1.067 1.501 -0.068
DH 98-27 x DB 533 -78.91 10.045 2.578 8.47** -0.2 0.685 -1.212 -0.042
178-24 x DB 533 -19.9 -7.291 3.868 3.78** 0.41** 0.566 6.697** 1.914**
DH46-1 x DB 533 345* -0.168 1.205 4.26** 0.26 -0.512 -1.196 -0.35
ZCH 8 x DB 533 380.94* 20.004 -0.382 -2.49* 0.55** -0.704 1.947 0.089
DH 37-4 x DB 533 226.05 -4.333 -0.259 -5.76** -0.16 0.073 3.384* 0.777
DH 24-4 x DB 533 -301.61 4.835 0.2 -0.51 -0.06 -0.62 1.167 0.033
DH 8-7 x DB 533 -93.5 2.667 -4.13 -6.33** -0.27 -1.708** -0.418 -0.801
DH 11-8 x DB 533 -336.54* 30.795 0.45 -0.95 0.09 1.175 -1.774 -0.005
DH 29-1 x DB 533 -35.57 14.085 2.531 4.03** 0.01 -0.333 1.437 0.188
DH 53-2 x DB 533 149.72 2.71 3.031 -2.18* 0.16 0.69 0.957 0.47
DH 35-17 x DB 533 -473.08** 15.083 -3.381 0.74 0.17 1.53* -2.791* -0.042
DH 23-4 x DB 533 -288.1 -44.038* -5.635* -6.37** -0.26 0.232 -2.171 -0.445
DH 31-2 x DB 533 -124.58 -33.33 -2.299 -7.41** -0.77** 0.858 0.573 0.502
DH 13-7 x DB 533 505.24** 14.292 -0.63 7.61** -0.23 -1.123 1.193 -0.152
DH 75- 23 x DB 533 -152.41 6.838 -0.134 -1.1 -0.53** 0.458 -2.388 -0.355
DH 91-1 x DB 533 -175.63 -9.457 -3.506 0.57 -0.25 1.203 -0.523 0.287
Table 8. Specific combining ability effects of derived F1 hybrids for various characters included in Line x Tester analysis
28. Crosses
Seed cotton
yield
(kg/ha)
Plant height
(cm)
No. of
sympodia
No. of bolls
Mean boll
weight (g)
Seed
index(g)
Ginning
outturn)%(
Lint index
(g)
1 2 3 4 5 6 7 8
DH 45-23 x DB 533 8.79 -14.956 0.741 0.34 -0.22 -0.913 -0.637 -0.516
DH 14-2 x DB 533 -148.42 4.04 2.034 3.09** 0.17 1.057 -4.126** -0.61
DH 82-3 x DB 533 -20.24 -0.541 0.324 3.24** 0.03 0.215 2.059 0.572
DH 23-21 x DB 533 -55.07 -14.498 -1.217 1.59 0.05 -0.122 -2.417 -0.692
DH 18-31 x DB 532 -69.82 52.601* 3.139 -0.59 -0.27 1.388* -2.804* -0.241
DH 49-1 x DB 532 58.74 -5.189 2.931 1.76 0.29 -0.299 0.917 0.065
DH 23-1 x DB 532 177.96 -8.233 0.764 8.61** -0.09 0.366 0.289 0.155
DH 98-27 x DB 532 352.59* -13.358 -1.569 -3.72** 0.13 -1.092 1.387 -0.163
178-24 x DB 532 -67.85 10.811 -3.444 -0.66 0.26 -0.401 -1.859 -0.657
DH46-1 x DB 532 4.3 -17.226 -1.446 -4.85** -0.32* -2.074** 0.683 -0.511
ZCH 8 x DB 532 311.09* 2.101 -1.694 -3.35** -0.15 0.604 0.166 0.298
DH 37-4 x DB 532 -6.46 9.269 -0.57 4.21** 0.08 0.076 -3.507** -0.725
DH 24-4 x DB 532 155.18 -2.733 -0.276 0.3 -0.07 -0.547 1.441 0.097
DH 8-7 x DB 532 10.32 -15.226 0.724 -0.27 -0.15 -0.31 -1.889 -0.572
DH 11-8 x DB 532 -214.96 -10.938 -0.361 -5.56** 0.15 -0.782 2.999* 0.429
DH 29-1 x DB 532 -15.83 -25.313 -2.44 -3.16** -0.06 -0.105 -3.459** -0.743
DH 53-2 x DB 532 321.29* -8.858 -3.61 -0.21 -0.15 -0.257 1.936 0.314
DH 35-17 x DB 532 35.66 7.855 1.972 -1.45 0.13 -0.467 3.708** 0.627
DH 23-4 x DB 532 -276.01 1.729 0.389 5.27** 0.31* 0.85 2.303 0.899*
DH 31-2 x DB 532 -64.25 24.772 5.055* 1.32 0.23 -0.814 -1.028 -0.565
DH 13-7 x DB 532 -328.12* -15.936 0.889 -2.5* -0.17 2.04** -0.813 0.557
29. Crosses
Seed cotton
yield
(kg/ha)
Plant height
(cm)
No. of
sympodia
No. of bolls
Mean boll
weight (g)
Seed
index(g)
Ginning
outturn)%(
Lint index
(g)
1 2 3 4 5 6 7 8
DH 75- 23 x DB 532 -15.6 5.77 2.055 3.3** -0.11 -0.564 1.836 0.164
DH 91-1 x DB 532 136.03 1.645 -2.983 -3.04** 0.13 0.131 -2.514 -0.49
DH 45-23 x DB 532 -67.75 -1.354 0.765 -1.1 -0.27 0.59 3.727** 1.188**
DH 14-2 x DB 532 -258.54 8.312 -1.943 -5.6** 0.12 -1.285* -1.327 -0.791
DH 82-3 x DB 532 -96.81 -14.439 -1.153 4.29** 0.23 0.343 -2.757* -0.52
DH 23-21 x DB 532 -81.17 13.939 2.806 6.98** -0.25 2.606** 0.567 1.187**
DH 18-31 x DB 531 -285.7 -67.461** -5.202 6.08** 0.25 -0.841 2.236 0.234
DH 49-1 x DB 531 14.05 8.914 -0.574 -2.91** -0.45** 0.398 0.002 0.13
DH 23-1 x DB 531 143.83 11.705 -1.242 0.36 -0.17 0.603 -1.921 -0.185
DH 98-27 x DB 531 -73.59 -15.92 -1.744 -3.31** -0.21 -0.231 -0.603 -0.258
178-24 x DB 531 345.95* -4.921 -1.954 -4.91** -0.13 -0.5 -4.999** -1.347**
DH46-1 x DB 531 39.28 9.206 -2.452 1.82 -0.16 0.153 2.658* 0.644
ZCH 8 x DB 531 -198.08 -24.626 -1.199 4.32** -0.01 0.56 -1.654 -0.127
DH 37-4 x DB 531 -25.34 -21.294 -0.576 -0.95 0.05 0.338 -1.017 -0.11
DH 24-4 x DB 531 293.36 -2.295 -0.787 2.05* 0.05 0.494 0.061 0.222
DH 8-7 x DB 531 -48.61 5.536 1.213 -0.26 0.35* 0.957 1.341 0.698
DH 11-8 x DB 531 -13.86 -1.34 -0.532 0.61 -0.21 -1.511* 0.604 -0.451
DH 29-1 x DB 531 -113.9 5.29 -0.451 -6.24** -0.19 -0.558 0.311 -0.148
DH 53-2 x DB 531 -456.72** -5.755 -1.116 -1.78 0.2 -0.176 -3.174* -0.756
DH 35-17 x DB 531 320.86* -11.047 2.297 8.22** -0.34* -0.706 1.073 -0.058
DH 23-4 x DB 531 1.94 30.161 1.718 0.45 -0.38* -0.313 1.068 0.124
30. Crosses
Seed cotton
yield (kg/ha)
Plant height
(cm)
No. of
sympodia
No. of bolls
Mean boll
weight (g)
Seed index(g)
Ginning
outturn)%(
Lint index
(g)
1 2 3 4 5 6 7 8
DH 31-2 x DB 531 44.88 43.87* 3.214 4.32** 0.27 0.468 -0.708 0
DH 13-7 x DB 531 -103.04 11.661 3.383 -2.16* 0.4** 0.047 -0.193 -0.018
DH 75- 23 x DB 531 -48.33 10.703 -1.451 -0.12 0.22 0.168 3.516** 0.874*
DH 91-1 x DB 531 -56.65 -4.587 2.007 -0.37 -0.04 -0.577 0.776 -0.04
DH 45-23 x DB 531 44.73 7.414 -0.241 0.07 0.29 1.272* -0.668 0.288
DH 14-2 x DB 531 202.16 -9.085 1.882 4.82** -0.12 0.257 2.263 0.619
DH 82-3 x DB 531 -133.61 12.499 3.342 -3.45** -0.25 0.934 -0.452 0.28
DH 23-21 x DB 531 106.39 11.371 0.466 -6.68** 0.57** -1.237* -0.518 -0.613
DH 18-31 x DB 534 -172.64 3.356 -0.057 -1.8 -0.63** 0.383 0.037 0.157
DH 49-1 x DB 534 -252.62 -1.604 -1.599 0.29 0 0.612 0.878 0.408
DH 23-1 x DB 534 -301.62 2.692 -2.767 -7.19** 0.03 0.097 0.13 0.098
DH 98-27 x DB 534 -200.1 19.232 0.736 -1.44 0.29 0.638 0.428 0.464
178-24 x DB 534 -258.19 1.401 1.531 1.79 -0.54** 0.335 0.162 0.09
DH46-1 x DB 534 -388.58* 8.189 2.693 -1.23 0.22 2.432** -2.146 0.217
ZCH 8 x DB 534 -493.95** 2.521 3.276 1.52 -0.39** -0.46 -0.458 -0.26
DH 37-4 x DB 534 -194.25 16.359 1.404 2.5* 0.02 -0.488 1.139 0.058
DH 24-4 x DB 534 -146.93 0.192 0.863 -1.83 0.07 0.673 -2.668* -0.351
DH 8-7 x DB 534 131.79 7.024 2.193 6.85** 0.07 1.061 0.967 0.675
DH 11-8 x DB 534 565.35** -18.518 0.443 5.9** -0.04 1.118 -1.83 0.027
DH 29-1 x DB 534 165.29 5.937 0.359 5.37** 0.23 0.996 1.712 0.704
DH 53-2 x DB 534 -14.29 11.902 1.694 4.17** -0.21 -0.257 0.282 -0.028
31. Crosses
Seed
cotton
yield
(kg/ha)
Plant
height
(cm)
No. of
sympodi
a
No. of
bolls
Mean boll
weight (g)
Seed
index(g)
Ginning
outturn
)%(
Lint index
(g)
1 2 3 4 5 6 7 8
DH 35-17 x DB 534 116.56 -11.89 -0.888 -7.5** 0.04 -0.357 -1.991 -0.526
DH 23-4 x DB 534 562.18
**
12.149 3.528 0.64 0.34* -0.769 -1.201 -0.578
DH 31-2 x DB 534 143.96 -35.313 -5.971* 1.77 0.27 -0.513 1.163 0.063
DH 13-7 x DB 534 -74.07 -10.016 -3.642 -2.96** 0 -0.964 -0.187 -0.386
DH 75- 23 x DB 534 216.34 -23.31 -0.471 -2.08* 0.42** -0.063 -2.963* -0.683
DH 91-1 x DB 534 96.26 12.4 4.482 2.83** 0.16 -0.758 2.262 0.243
DH 45-23 x DB 534 14.23 8.896 -1.266 0.69 0.21 -0.949 -2.422 -0.96*
DH 14-2 x DB 534 204.8 -3.268 -1.973 -2.31* -0.16 -0.029 3.189* 0.782
DH 82-3 x DB 534 250.65 2.481 -2.513 -4.08** -0.01 -1.492* 1.149 -0.332
DH 23-21 x DB 534 29.84 -10.811 -2.054 -1.9 -0.38* -1.248* 2.368 0.119
SEd 154.00 20.39 2.48 0.99 0.15 0.61 1.31 0.42
CD at 5% 431.23 40.50 4.92 2.76 0.41 1.21 2.61 0.84
CD at 1% 572.80 53.65 6.51 3.67 0.55 1.60 3.46 1.12
* Significant at P = 0.05 ** Significant at P = 0.01
32. 2 .Mean per se performance of best HB
interspecific hybrids
33. Sl. No.
Hybrids
Seed
cotton
yield
(kg/ha)
Plant height
(cm)
No. of mono
podia
No. of sym
podia
No. of
bolls
Mean boll
weight (g)
Repro duction
Points on
Sympodia
Length of
sympo dia
at 50%
height
Inter
Branch
Distant
(cm)
Seed
index (g)
Ginning
outturn
(%)
Lint
index
(g)
2.5% SL
(mm)
%
Micronaire
value
(µg/inch)
Tenacity
(g/t)
Elon gation
%
Value Rank BMC 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
1 DH 102-23 x DB 534 1956.88 26 301.00 1.33 30.67 21.00 4.90 7.00 89.50 37.00 13.00 34.33 4.46 36.60 44.00 2.70 24.90 6.10
2 DH 39-84 x DB 531 1781.82 38 231.33 1.67 33.67 27.00 5.20 5.00 57.50 29.33 13.00 27.76 3.61 35.50 44.00 3.40 24.20 6.20
3 DH 22-76 x DB 534 1687.00 42 234.00 2.33 29.67 47.33 4.30 7.00 71.17 29.67 11.00 38.25 4.21 35.20 47.00 3.10 26.40 6.70
4 DH 57-95 x DB 533 1787.03 37 234.67 1.00 33.33 32.00 4.70 7.00 70.33 29.33 11.00 37.66 4.14 35.10 45.00 3.20 25.60 6.40
5 DH 11-8 x DB 532 1797.45 34 209.67 3.67 29.00 27.00 5.20 5.17 70.67 27.33 13.00 31.07 4.04 36.50 46.00 3.30 25.20 6.40
6 DH 28-36 x DB 531 1963.13 25 252.33 2.33 31.00 33.33 4.60 5.33 72.67 30.00 15.00 34.38 5.16 38.90 43.00 2.90 25.70 6.10
7 DH 98-27 x DB 533 2130.89 10 BMC 201.33 2.00 27.00 31.00 4.60 4.83 57.50 27.67 13.00 35.29 4.59 35.30 47.00 3.30 25.40 6.30
8 DH 18-31 x DB 532 1859.97 32 178.00 2.00 23.00 27.00 4.50 4.83 55.50 28.67 12.00 37.46 4.50 34.20 46.00 3.20 25.40 6.20
9 DH 47-67 x DB 532 1640.11 45 229.67 3.67 28.00 29.33 4.40 4.83 58.83 29.33 11.00 34.73 3.82 38.20 44.00 3.00 24.20 5.90
10 DH 40-29 x DB 532 2052.74 19 257.67 2.67 39.33 38.00 3.80 5.50 66.83 26.67 12.00 35.10 4.21 34.00 46.00 3.30 25.50 6.30
11 DH 18-31 x DB 533 2216.33 4 BMC 204.00 0.67 31.00 31.67 3.60 4.67 52.00 27.67 12.00 38.11 4.57 33.90 45.00 3.10 25.40 6.20
12 178-24 x DB 533 2151.73 8 BMC 258.67 1.33 34.00 34.00 4.30 6.50 63.67 30.00 12.00 34.54 4.14 36.40 45.00 3.00 24.90 5.90
13 DH 98-27 x DB 534 2239.26 2 BMC 236.00 1.67 28.00 21.67 5.10 6.50 66.83 29.33 13.00 36.12 4.70 35.40 47.00 3.00 27.30 6.30
14 DH 11-20 x DB 534 1232.69 49 202.67 2.67 30.33 27.33 4.30 6.17 62.00 27.67 12.00 34.31 4.12 38.90 44.00 2.80 25.70 6.00
15 DH 55-2 x DB 531 1795.37 35 223.33 1.33 29.00 35.33 4.20 6.67 70.67 34.67 14.00 36.04 5.05 36.20 46.00 2.60 25.60 6.00
16 ZCH 8 x DB 533 2277.81 1 BMC 227.00 2.00 29.33 30.00 4.70 4.50 53.33 28.33 11.00 34.64 3.81 35.60 44.00 3.20 26.50 6.40
17 DH 95- 18 x DB 533 2005.85 20 201.33 2.33 24.00 29.67 5.10 4.67 59.00 30.00 14.00 31.93 4.47 40.00 44.00 2.90 23.50 5.90
18 DH 53-2 x DB 534 1874.56 31 234.33 2.00 33.00 33.00 5.20 5.33 67.67 27.00 14.00 31.25 4.38 40.20 43.00 2.80 24.00 5.90
19 DH 43-44 x DB 532 1648.44 44 227.67 4.00 31.67 32.00 3.70 6.33 67.17 30.67 13.00 31.69 4.12 38.10 44.00 2.60 25.50 6.00
20 DH 53-2 x DB 533 1564.04 46 239.67 3.00 27.00 30.67 3.80 4.83 58.17 32.33 14.00 32.81 4.59 36.90 46.00 2.90 25.50 6.00
21 DH 18-31 x DB 534 2101.71 11 BMC 239.67 2.33 25.33 32.67 4.90 6.00 68.17 28.33 13.00 31.25 4.06 37.50 45.00 3.20 23.70 5.90
22 DH 21-29 x DB 532 1775.57 39 230.00 2.33 26.67 31.00 4.60 4.33 60.50 34.00 12.00 32.54 3.90 38.10 45.00 3.30 23.20 5.80
23 DH 25-22 x DB 533 1718.26 41 276.00 3.00 27.33 23.33 4.30 7.33 87.17 32.00 12.00 31.52 3.78 40.30 43.00 2.50 24.30 5.80
24 DH 18-19 x DB 533 1333.76 48 253.33 3.33 31.67 35.67 4.60 5.00 60.00 32.00 14.00 29.45 4.12 39.20 43.00 2.80 25.10 5.90
25 DH 29-32 x DB 531 1990.22 23 263.33 2.67 30.67 22.67 4.80 6.17 68.83 33.00 13.00 33.43 4.35 37.90 44.00 3.30 23.10 5.90
26 ZCH 8 x DB 532 1886.02 30 229.67 1.67 32.67 27.33 4.30 5.33 78.83 27.67 12.00 33.50 4.02 36.40 46.00 3.20 25.50 6.30
27 DH 11-10 x DB 534 2136.10 9 234.00 3.00 37.33 31.67 4.70 6.17 61.67 29.33 13.00 34.17 4.44 37.30 46.00 3.30 24.20 6.10
28 DH 66 x DB 534 1793.28 36 233.00 3.00 28.00 32.00 3.90 6.00 68.17 31.00 13.00 30.20 3.93 40.20 45.00 2.80 24.30 5.80
Table 9. The mean performance of best HB hybrids in respect of seed cotton yield and its attributing characters