Improving sorghum productivity in
semi-arid environments of Mali
through integrated MARS
G4008.48
Products, challenges & l...
MARS: increase the exploration of recombination
potential in bi-parental population
Breed for :
multiple traits
polygenic ...
Lata3 KeninkeniTiandougouX X
Parents chosen for MARS are elite varieties with
complementary traits
Guinea type
Medium size...
The project in a
nutshell
Keninkeni Lata3
Population 114 Population 118
x x
F4 Families
(10 plants)
…
…
F4 seed increase (...
• 3 locations : Sotuba, Cinzana, Farako
• 2 sowing dates in each location
• Multiple traits
• Yield and components
• Flowe...
Challenges: multilocation phenotyping
Grain yield
Grain quality
Grain quality
Grain yield
Challenges: QTL detection and ideotype
definition
Challenges: Genotyping data management
Track inconsistent genotyping data in pedigrees
• Identify:
• Wrong crosses
• Selfi...
Challenges :
From crosses ranking to crossing
Keninkeni Lata3
Population 114 Population 118
x x
…
F4 seed increase (F5 Bulk)
Phenotyping
3 sites x 2 sowing dates
1 site...
Main product: improved material
P114 Pedigree
Alelles selection over cycles
Evolution of index value over cycles
P114 – C1...
Strategy for variety development
Year Type Sub type
Nb of
entries Nb of Rep Nb of envt Nb of Plot Designation
2013 Screeni...
Products: Grain quality phenotyping
• Improvement and transfer
to IER of NIRS calibrations
• Protein content
• Lipid conte...
Capacity building
• Two PhD students:
• Dramane Sako (Mali) – Genetics of panicle
architecture
WACCI – Ends early 2014
• D...
Lessons learned
MARS proof of concept: a valid procedure for
sorghum breeding in Africa.
• Pitfall: “eggs in same basket” ...
Summary
• Successful integration of : - Methodology
- Tools/services
- Capacity Building
into sorghum national breeding pr...
Baptiste Guitton
Elodie Lacut
Michel Vaksmann
Jean-Francois Rami
Denis Lespinasse
Michel Ragot
IER
Niaba Témé
Mamoutou Kou...
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GRM 2013: Improving sorghum productivity in semi-arid environments of Mali through integrated MARS: Products, challenges & lessons learnt -- J-F Rami

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GRM 2013: Improving sorghum productivity in semi-arid environments of Mali through integrated MARS: Products, challenges & lessons learnt -- J-F Rami

  1. 1. Improving sorghum productivity in semi-arid environments of Mali through integrated MARS G4008.48 Products, challenges & lessons learned J-F Rami – General Research Meeting – Lisbon – 27th - 30th Sep 2013
  2. 2. MARS: increase the exploration of recombination potential in bi-parental population Breed for : multiple traits polygenic traits diverse environments... ...Towards an ideal genotype cumulating favourable alleles from both parents for all genes involved in different target traits X Parent 1 Parent 2 X Parent 1 Parent 2 GV GV GV GV GV GV GV GV GVGV GV GV GV QTL detection Multiple traits Multiple environments GV
  3. 3. Lata3 KeninkeniTiandougouX X Parents chosen for MARS are elite varieties with complementary traits Guinea type Medium size Photoperiod sensitive Tillering Caudatum type Short size Photoperiod sensitive High productivity GC type Medium size Photoperiod sensitive Good grain quality 400 F3 400 F3
  4. 4. The project in a nutshell Keninkeni Lata3 Population 114 Population 118 x x F4 Families (10 plants) … … F4 seed increase (F5 Bulk) Phenotyping 3 sites x 2 sowing dates 1 site for grain quality F1 F2 F3 S2 S3 S4 Dec. 2008 March 2009 June 2009 F4 Families (10 plants) Dec. 2009 Feb. 2010 QTL analysis Ideotype definition Phenotyping 3 sites x 2 sowing dates 1 site for grain quality F3 genotyping QTL analysis Ideotype definition Genotyping C1 cycle (F4 families) C1 genotyping C1 crosses June 2010 Jan. 2011 June 2011 Jan. 2012 C1 seed increase (F2) June 2012 C1 evaluation (F3) Tiandougou C2 seed increase (F2) C1 + C2 evaluation (F2) Jan. 2013 June 2013 C1 cycle (F4 families) C1 genotyping C1 crosses C2 cycle (C1 families) C2 genotyping C2 crosses C3 cycle (C2 + C1 families) C3 genotyping C3 crosses F4 seed increase (F5 Bulk) June 2008 F1 F2 F3 S2 S3 S4
  5. 5. • 3 locations : Sotuba, Cinzana, Farako • 2 sowing dates in each location • Multiple traits • Yield and components • Flowering date • Plant morphology • Panicle morphology and architecture • Grain quality (NIRS + mini Tô) Challenges: multilocation phenotyping
  6. 6. Challenges: multilocation phenotyping
  7. 7. Grain yield Grain quality Grain quality Grain yield Challenges: QTL detection and ideotype definition
  8. 8. Challenges: Genotyping data management Track inconsistent genotyping data in pedigrees • Identify: • Wrong crosses • Selfing • Very short timeframe
  9. 9. Challenges : From crosses ranking to crossing
  10. 10. Keninkeni Lata3 Population 114 Population 118 x x … F4 seed increase (F5 Bulk) Phenotyping 3 sites x 2 sowing dates 1 site for grain quality F1 F2 F3 S2 S3 S4 Dec. 2008 March 2009 June 2009 Dec. 2009 Feb. 2010 QTL analysis Ideotype definition Phenotyping 3 sites x 2 sowing dates 1 site for grain quality F3 genotyping QTL analysis Ideotype definition Genotyping C1 cycle (F4 families) C1 genotyping C1 crosses June 2010 Jan. 2011 June 2011 Jan. 2012 C1 seed increase (F2) June 2012 C1 evaluation (F3) Tiandougou C2 seed increase (F2) C1 + C2 evaluation (F2) Jan. 2013 June 2013 C1 cycle (F4 families) C1 genotyping C1 crosses C2 cycle (C1 families) C2 genotyping C2 crosses C3 cycle (C2 + C1 families) C3 genotyping C3 crosses F4 seed increase (F5 Bulk) June 2008 F1 F2 F3 S2 S3 S4 Main product: improved material 724 F4 Plants (20 Families) 140 SNP 130 C1 crosses 105 Selfing 647 C1 Plants (23 Families) 140 SNP 125 C2 crosses 70 Selfing 558 C2 Plants (19 C2 Families + 13 C1 families) 150 SNP 20 C3 crosses 60 Selfing 760 F4 Plants (30 Families) 158 SNP Crosses starting in two weeks Population 114 Population 118
  11. 11. Main product: improved material P114 Pedigree Alelles selection over cycles Evolution of index value over cycles P114 – C1 Phenotyping Evaluation 2 locations x 2 reps x 2 sowing dates
  12. 12. Strategy for variety development Year Type Sub type Nb of entries Nb of Rep Nb of envt Nb of Plot Designation 2013 Screening trials Station 64 2 6 768 C2P114 2013 Variety trials Station 6 4 3 72 2013 Variety trials Farmers 10 1 30 300 2014 Screening trials Station 64 2 6 768 C1P118 2014 Screening trials Station 64 2 6 768 C3P114 2014 Variety trials Station 10 4 3 120 2014 Variety trials Farmers 10 1 30 300 2015 Screening trials Station 64 2 6 768 C2P118 2015 Variety trials Station 15 4 3 180 2015 Variety trials Farmers 10 1 30 300 2016 Screening trials Station 64 2 6 768 C3P118 2016 Variety trials Station 20 4 3 240 2016 Variety trials Farmers 10 1 30 300 2017 Variety trials Station 25 4 3 300 2017 Variety trials Farmers 10 1 30 300
  13. 13. Products: Grain quality phenotyping • Improvement and transfer to IER of NIRS calibrations • Protein content • Lipid content • Amylose content • Endosperm texture • Grain image analysis Grain roundness Grain color Diarah Guindo
  14. 14. Capacity building • Two PhD students: • Dramane Sako (Mali) – Genetics of panicle architecture WACCI – Ends early 2014 • Diarah Guindo (Mali) – Genetics of grain quality End Oct 2014 • Two MSc students: • Korotimi Thera – Flowering and photoperiod sensitivity modelling and genetics • Mohamed Tekete – Flowering time in BCNAM populations • All part of the IBP Multi Year Course
  15. 15. Lessons learned MARS proof of concept: a valid procedure for sorghum breeding in Africa. • Pitfall: “eggs in same basket” approach • Broad genetic basis: some genes with large effect • Multiple traits, multiple environments • Relations between traits, differential expression of QTLs in various environments. • Break some possible antagonisms between traits, test different hypothesis, design various ideotypes for various environments..
  16. 16. Summary • Successful integration of : - Methodology - Tools/services - Capacity Building into sorghum national breeding programme in Mali • Extensive evaluation to come toward variety development • Significant change in the way of conceiving/conducting breeding • Capacity building
  17. 17. Baptiste Guitton Elodie Lacut Michel Vaksmann Jean-Francois Rami Denis Lespinasse Michel Ragot IER Niaba Témé Mamoutou Kouressy Sidy Bekaye Coulibaly Flakoro Coulibaly Yacouba Dembélé Mohamed Doumbia Mahamady Kané Korotimi Thera Dramane Sako Diarah Guindo

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