This document provides information on various viral and bacterial vaccines. It discusses the smallpox, rabies, yellow fever, influenza, polio, measles, mumps, typhus, and combination virus vaccines. For each it describes the source, preparation, storage, uses, and dose. It also covers bacterial vaccines for typhoid, cholera, pertussis, plague, and BCG. The document emphasizes that vaccines are prepared from viruses or bacteria grown in cells or egg embryos, then inactivated or attenuated before use. Combination vaccines provide protection against multiple diseases in a single shot.

1. VACCINES
Content is related to pharmacognosy
Prepared by : Urooj Umer

2. contents
Biologics (vaccines, toxins, antitoxins, venoms, antivenoms, antiserum)
2 . Vaccines
VIRAL VACCINES
• small pox vaccine
• Rabies vaccine
• Yellow fever vaccine
• Influenza vaccine
• Poliomyelitis vaccine
• Measles and mumps vaccine
• Rickettsial vaccine
• Typhus vaccine
• Combination virus vaccine
BACTERIAL VACCINES
• Typhoid vaccine
• Cholera vaccine
• Pertussis vaccine
• Plague vaccine
• BCG vaccine

3. biologics
“A biologic drug (biologics) is a product that is produced from living organisms
or contain components of living organisms”
The broad term “ BIOLOGICS” , includes the “IMMUNIZING BIOLOGICS” that
are the derivatives of both animals and plants.
 Derivatives of animals (serum , antitoxins , globulins)
 Derivatives of plants (vaccines , toxins , toxoids , tuberculins)
They directly or indirectly confer a state of protection against pathogenic microorganisms.
Biologics are not classified as chemotherapeutic agents or antibiotics.
Biologics are classified into two general categories :
antigen
antibody

4. Antigen and antibody
Antigen
“a toxin or other foreign substance which induces an immune response in the body,
especially the production of antibodies.”
Antigen can also be defined as:
Antibody
“a blood protein produced in response to and counteracting a specific antigen. Antibodies
combine chemically with substances which the body recognizes as alien, such as bacteria,
viruses, and foreign substances in the blood.”
It is a large Y shaped molecule that neutralize the pathogens.
It has following five types:
Biologically Chemically Physically
igM igA igE igD igG

5. Antibody structure

6. vaccines
“A substance used to stimulate the production of antibodies and provide immunity
against one or several diseases, prepared from the causative agent of a disease.”
 vaccines may contain living , attenuated , or killed virus , attenuated or killed bacteria
and they are used as inoculations to stimulate the production of antibodies.
 The general action of vaccine should be considered prophylactic.
 Non living vaccine provide protection for limited time and repeated vaccine is
required to provide protection against typhoid fever, plaque, cholera and typhus.
 Active immunization is the induction of immunity after exposure to an antigen.

7. Immunity and
vaccination

8. Immune system

9. Cultivation of viral culture
The cultivation of virus poses a problem because they completely depend
on living organisms for their sustenance .
• Vaccines are obtained from many animals.
The use of chick or duck embryos for viral culture offers
advantages in some cases , but the development of techniques for tissue
culture of mammalian cells provide major basic advancement necessary
for significant expansion in practical use of many viral vaccines.
• The vaccines are also grown on tissue cultures prepared from chick
embryo , monkey kidney or human diploid cells .

10. Types of vaccines
• There are two types of vaccines.
VACCINES
VIRAL VACCINES BACTERIAL VACCINES
First of all I am going to discuss VIRAL VACCINES
• Viral vaccines are used to kill viruses .
• Living viral vaccines are used for prophylaxis against mumps , rubella , small pox
Nonliving or inactivated or killed vaccines are available for rabies and influenza.

11. Points to be discussed
• Sources
• Preparation
• Dose
• Storage
• Uses

12. SMALLPOX VACCINE
“The smallpox vaccine protects people from smallpox by
helping their bodies develop immunity to smallpox.
The vaccine is made from a virus called vaccinia, which is a
poxvirus similar to smallpox, but less harmful.”
Sources of vaccine:
It is the living virus of vaccinia (cow pox) that has been grown in the
skin of a vaccinated bovine calf.

13. CONTINUE…
Preparation of vaccine:
The vaccine is prepared by skin of calf . The calf is prepared by washing and shaving
its belly, then scarifying so that the serum oozes through the cuts. The seed virus is
then inoculated into the scarifications merely by hand rubbing. The calf is maintained
in aseptic conditions. The vesicles that develop are removed at the time of maximum
potency, thoroughly triturated and either made into smooth suspension with any
aqueous solution of glycerin or sorbitol or reduced to dried pellet. The animal must
have good health prior to inoculation . After the virus is harvested , the animal is
killed .
Storage of vaccine:
liquid vaccine is stored below 0 ̊c because it loses its potency at high temperature.
Dried vaccine is stored at temperature between 2 and 8 ̊c

14. CONTINUE…
Uses and dose:
Small pox vaccine is a specific immunizing agent and is used as prophylactic
before the infection occurs . It creates any immunity that lasts for about 7 years.
Usual dose , is percutaneous contents of 1 capillary tube by multiple – puncture
method.

15. RABIES VACCINE
“Rabies vaccine is a sterile preparation of killed , fixed
virus of rabies in dried form.”
Sources of vaccine:
2 Modern rabies vaccines, made from cell-culture or duck-
embryo-derived rabies virus which is then purified and
inactivated, are replacing the older vaccines produced in
brain tissue

16. CONTINUE…
Preparation of vaccine:
This vaccine is prepared in primary chick embryo cells derived from specific
pathogen-free (SPF) eggs. It is a freeze-dried preparation consisting of purified and
concentrated rabies virus antigen inactivated with B-propiolactone.
Rabies vaccine of duck embryo origin contains little or no myelin, the “the
paralytic factor” that causes rabies treatment paralysis. Duck vaccine is sometimes
called “avianized” vaccine.
The vaccine from brain tissue contains significant amount of myelin.
Diploid rabies vaccine is a new vaccine prepared from virus grown in
cultures of human diploid embryo lung tissue. Because it contains less protein
then duck embryo.

17. CONTINUE…
Storage of vaccine:
It should be stored at temperature between 2 and 8 ̊c.
Uses and dose:
The vaccine is an active immunizing agent and is recommended primarily for
prevention of rabies in persons bitten by rabid animal.
The usual administration is a subcutaneous injection
once a day for 14 days.

18. Yellow fever vaccine
“Yellow fever vaccine is an attenuated strain of living
yellow fever virus , selected for highly antigenic activity .”
The other name of this vaccine is 17D vaccine.
SOURCE:
The yellow fever vaccine is made by growing yellow fever virus
in mouse embryo cells and in chick embryo cells. The final
preparation of the vaccine is made in eggs.

19. CONTINUE…
Preparation of vaccine:
It is prepared by culturing the virus in the living embryo of domestic fowl. The
virus-infected, chick-embryo pulp is suspended in water and, after appropriate
aseptic processing it is distributed in suitable quantities into amplus and dried from
the frozen state. After-ward, the amplus are filled with dry nitrogen and flame –
sealed.
Storage of vaccine
it is preferably store at temperature below 0 ̊c but never above 5 ̊c.

20. CONTINUE…
Uses :
 This vaccine is used to help prevent yellow fever in adults and children
who are at least 9 months old
 The vaccine provides effective immunity within 30 days for 99
percent of those vaccinated. Travel to affected areas should not begin
 before this time.
 One vaccination of yellow fever vaccine is enough to build life-long
immunity against Yellow Fever.
Dose
The usual dose given subcutaneously is 0.5 ml

21. Influenza vaccine
“Influenza virus vaccine is a sterile aqueous suspension of
suitably inactivated influenza virus types A and B either
individually or combined or virus subunits prepared from extra
embryonic fluid of influenza virus.”
Sources of vaccine:
It is made by growing the viruses inside fertilized chicken eggs.
This means that they contain a small amount of egg protein.

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Preparation of vaccine:
The strains of influenza virus used in the preparation of this vaccine are those
designated for the particular season . It contains a suitable preservative and may contain
an adsorbent such as aluminum phosphate or protamine. During the commercial
preparation of the vaccine, the virus growths are collected, concentrated, refined by
ultracentrifugation, and inactivated by ultraviolet irradiation. These CVVs are then
injected into fertilized hen's eggs and incubated
for several days to allow the viruses to replicate. The fluid containing
virus is harvested from the eggs. Each lot of influenza virus vaccine must be tested to
determine its potency; its power to stimulate the formation of specific virus-neutralizing
antibodies in mice is correlated with the potency.

23. CONTINUE…
Storage of vaccine:
the storage temperature is between 2 and 8 ̊c
Uses & Dose:
Influenza virus vaccine is an active immunizing agent.
Its usual dose is 0.5 ml intramuscularly, preferably in the Span-deltoid
muscle.

24. Poliomyelitis VACCINE
“Poliomyelitis vaccine inactivated is a sterile suspension
of inactivated poliomyelitis virus of types 1, 2 , 3.”
Sources of vaccine:
IPV. The first inactivated polio vaccine
(IPV) was produced by Salk using
virus grown on monkey kidney
cells and inactivated with formalin.

25. CONTINUE…
Preparation of vaccine:
The Salk vaccine, IPV, is based on three wild, virulent reference
strains, Mahoney (type 1 poliovirus), MEF-1 (type 2 poliovirus), and
Saukett (type 3 poliovirus), grown in a type of monkey kidney tissue
culture (Vero cell line), which are then inactivated with formalin. The
injected Salk vaccine confers IgG-mediated immunity in the
bloodstream, which prevents polio infection from progressing
to viremia and protects the motor neurons, thus eliminating the risk
of bulbar polio and post-polio syndrome.

26. CONTINUE…
Storage of vaccine:
Vaccine is potent if stored at not higher than -20°C until the expiry date indicated on
the vial. It can be stored for up to six months between +2°C and +8°C.
Uses and dose of vaccine:
The usual administration schedule involves an initial administration of 2
doses at not less than 8-week intervals. A third, reinforcing dose is
administered to 12 months later. The volume of vaccine indicated on the
label as representing one dose is generally placed on a cube of sugar, which
is eaten by the individua! to be immunized.

27. MUMPS AND MEASLES VACCINE
“Vaccines containing live attenuated rubeola (measles) and
rubella (German measles) viruses are available for active
immunization.”
Other name : MMR vaccine
Sources:
Vaccine live is prepared with
the B-level Jeryl Lynn
strain of the virus,
which is grown
in cell cultures
of chicken embryo tissue.
mumpsmeasles

28. CONTINUE…
Preparation of vaccine:
It's grown in cultures of chick embryos basically, unhatched live eggs. Lots
of vaccines are grown that way it's old-fashioned technology but it works.
A 25 gauge, 5/8" needle is recommended. To reconstitute, use only the diluent
supplied, since it is free of preservatives or other antiviral substances which might
inactivate the vaccine. Single Dose Vial — First withdraw the entire volume of diluent
into the syringe to be used for reconstitution.
Storage of vaccine:
M-M-R®
II must be stored between -58°F and +46°F (-50°C to +8°C). Use of
dry ice may subject M-M-R®
II to temperatures colder than -58°F (-50°C). Before
reconstitution, store the lyophilized vaccine at 36°F to 46°F (2°C to 8°C).

29. CONTINUE…
Uses and dose:
MMR vaccine is very effective at protecting people against measles, mumps, and rubella,
and preventing the complications caused by these diseases. People who received two
doses of MMR vaccine as children according to the U.S. vaccination schedule are usually
considered protected for life and don't need a booster dose.
It provides active immunity for at least 10 years after immunization and is particularly
valuable to susceptible individuals approaching puberty and to adults.

30. RICKETTSIAL VACCINE
“Two types
of vaccines against Rickettsial infections have been
developed and used in humans, whole killed bacteria
and live attenuated rickettsiae.”
Sources of vaccine:
it is obtained from chicken embryo or monkey kidney.

31. CONTINUE…
Preparation of vaccine:
Rickettsiae are cultured in chick embryos or in monkey kidney tissue cultures
in a manner similar to that for viruses. They cannot be grown in artificial
culture media and must be subjected to the same precautions as viruses. At
the present time, Rickettsial vaccines are not produced commercially in the
United States.
Storage of vaccine:
It is stored at 4 ̊c.
Dose:
it is given 100 mg twice per day.

32. TYPHUS VACCINE
“Typhus vaccine is a sterile suspension of killed rickettsial
organisms of strain or strains of epidemic typhus rickettsiae
selected from antigenic efficiency.”
Sources of vaccine:
It is obtained by culturing the yolk sac membrane of developing
embryo of the domestic fowl and the rickettsiae are killed by suitable
chemical agents.

33. CONTINUE…
Preparation of vaccine:
One typhus vaccine consists of formaldehyde-inactivated Rickettsia prowazekii.
Two doses are injected subcutaneously four weeks apart. Booster doses are
required every six to twelve months.
Storage of vaccine:
Vaccine potency is dependent upon storage under refrigeration [between 2 °C
and 8 °C (35.6 °F– 46.4 °F)]. The vaccine should be stored under refrigeration at
all times. It is essential to replace unused vaccine in the refrigerator between
doses. The expiration date is not later than 18 months after the date of issue.

34. CONTINUE…
• Uses and dose:
An active immunizing agent for epidemic typhus fever is administered as a
prophylactic measure particularly to travelers.
The usual administration involves two subcutaneous injections of 0.5 ml 4 or more
weeks apart , followed by 0.5 ml every 6 to 12 months for as long as protection is
desired.

35. COMBINATION VACCINES
“Combination vaccines take two or
more vaccines that could be given individually and
put them into one shot. Children get the same
protection as they do from
individual vaccines given separately—but with
fewer shots.”
These combination vaccines are administered
subcutaneously to children 15 months of age or older.
Use in infants under 15 months of age is not
recommended

36. CONTINUE…
Uses
Combination vaccines combine protection against 2 or more diseases into
one shot. The measles, mumps, and rubella vaccine (MMR) and diphtheria,
tetanus ,and pertussis vaccine (DTaP) each protect your child against three
diseases.

37. BACTERIAL VACCINES
Bacterial vaccines consist of suspensions of attenuated or , more
commonly killed pathogenic bacteria in isotonic sodium chloride
solution or other suitable diluents.
Properties
Strains must be selected on the basis of high antigenicity.
The smooth “S” strains are uniformly more antigenic than rough
“R” strains.

38. Typhoid vaccine
“Typhoid vaccine is a sterile suspension containing killed
typhoid bacilli of a strain selected for high antigenic efficiency.”
Sources of vaccine:
It contains 1 billion typhoid
organisms
in each ml and not
more than 35µg of total nitrogen.

39. CONTINUE…
Preparation of vaccine:
Heat inactivated phenol preserved vaccine (TAB vaccine): Prepared by
heating S. typhi, S. Para typhi A and S. Para typhi B cultures at 56oC for one
hour and then suspending in 0.5% phenol solution.
Storage of vaccine:
The vaccine should be stored at temperature
2 to 8 ̊ c.
Uses and dose:
It is active immunizing agent against typhoid fever.
The immunization schedule involves two 0.5 ml
subcutaneous injections at least 4 years apart.
After that 0.5 ml every three years thereafter.

40. CHOLERA VACCINE
“Cholera vaccine is a sterile suspension of killed cholera
vibrios ( Vibrio cholera ) in isotonic sodium chloride
solution or other suitable diluent.”
Sources of vaccine:
A vaccine consisting of killed whole-cell V.
cholerae O1 in combination
with a recombinant
B-subunit of cholera toxin .

41. CONTINUE…
Preparation of vaccine:
It is prepared from equal portions of suspensions of cholera vibrios of inaba and ogawa
strains. At the time of manufacture , cholera vaccine contains approximately 8 billion
cholera organisms in each ml.
Storage of vaccine:
The vaccine should be stored at a temperature between 2 to 8 ̊c.
Uses and dose:
It is an active immunizing agent in the development of immunity .
Its dose in adult is 0.5 ml intramuscularly or subcutaneously
and then 1ml 4 weeks later .
0.5 ml dose is repeated every 6 months.

42. PERTUSSIS VACCINE
“It is a sterile bacterial fraction or suspension of killed
pertussis bacilli ( Bordetella pertussis) of a strain or strains
selected for high antigenic efficiency.”
Pertussis vaccine is a vaccine that protects against whooping cough
(pertussis).
There are two types :whole cell vaccine and acellular vaccine.
The whole cell vaccines about 78%
effective while the acellular vaccine
about 71-85 %effective.

43. CONTINUE…
Preparation of vaccine:
The pertussis vaccine is made by taking two to five of these toxins and inactivating
them with a chemical. The inactivated toxins are called "toxoids." Once injected,
the toxoids elicit an immune response against the toxins, but, unlike the toxins,
they don't cause disease.
Absorbed pertussis vaccine consist of pertussis vaccine that has been
precipitated or adsorbed by the addition of aluminum hydroxide or aluminum
phosphate. It is same as pertussis vaccine but has more than 850µg of aluminum in
the volume stated in the labeling constitute of 1 injection.

44. CONTINUE…
Storage of vaccine:
pertussis vaccines refrigerated between 2°C and 8°C (36°F and 46°F)
Uses and dose:
Pertussis vaccine is an active immunizing agent used to treat immunity.
The Tdap vaccines protects teens and adults from tetanus,
diphtheria and whooping cough.
The usual dose is 3 injections usually 0.5 ml or 1ml
as specified at least 3to 4 weeks apart.

45. PLAGUE vaccine
“It is a sterile suspension in an isotonic sodium chloride solution
or other suitable diluent of killed plague bacilli ( Yersinia pestis)
of a strain selected for high antigenic efficiency.”
Sources of vaccine:
It contains 2 billion plague bacilli in each ml.

46. CONTINUE…
Preparation of vaccine:
Recommended antimicrobials include tetracyclines, chloramphenicol, or
streptomycin. The plague vaccine licensed for use in the United States
is prepared from Y. pestis organisms grown in artificial media, inactivated with
formaldehyde, and preserved in 0.5% phenol.
Storage of vaccine:
It is stored at standard indoor room temperatures, usually considered to be
between 20°C and 25°C (68°F and 77°F).

47. CONTINUE…
Uses and dose:
Plague vaccine is used to develop immunity against disease.
It is restricted to travelers .
Its dose include two 0.5 ml intramuscular injections ,
at least 4 weeks apart, then 0.2 ml 4 to 12 weeks later

48. BCG VACCINE
“Bacillus Calmette–Guerin vaccine is a vaccine primarily
used against tuberculosis. It is a freeze dried preparation of
the culture of an attenuated strain of bovine tuberculosis.”
Sources of vaccine:
The vaccine was originally developed from Mycobacterium
bovis, which is commonly found in cows ,while it has been
weakened ,it is still live.

49. CONTINUE…
Preparation of vaccine:
The bcg vaccine is prepared from a weakened strain of mycobacterium bovis.
Many foreign born person have been BCG vaccinates .BCG vaccine is used in
many countries with a high prevalence of TB to prevent childhood
tuberculous meningitis and milliary disease.
Storage of vaccine:
The expiration date of BCG vaccine is up to one year , if stored at 5 ̊c.

50. CONTINUE…
Uses and dose:
BCG vaccine provides immunity or protection against Tuberculosis(TB) .The
vaccine may be given to persons at high risk of developing TB It also used to
treat bladder Tumors or bladder cancer .This medication is sometime
prescribed for other uses to treatment many bacterial infection diseases.
It is administered intradermally as a reconstituted vaccine in the doses of 0.1
ml.

51. REFRENCES
Pharmacognosy (tenth edition)
Tyler , Varro E
www.slidesharenet.com
www.vzccines.prezzi.com
www.slidesplayervaccines.com