Use of Novel combination adjuvant platform technology for vaccines against Pertussis and Respiratory Syncytial Virus (RSV) infections. The potential of combination vaccines was further potentiated using Nanotechnology.

1. Srinivas Garlapati
Vaccine and Infectious Disease Organization
Saskatoon, Canada
Optimizing vaccine formulation and delivery to improve human and animal health
Partnership development activity (PDA) between Canada and India
funded by ISTP, Canada

2. Courtesy: Parallel solutions IncAndrianov et al, 1998

3. 1000X 5000X
OVA CpG Merge
Garlapati et al 2008
Garlapati et al 2010

4. Alum SOL
MP
MP

5.  Single step process by simple mixing PCEP with natural
poly-amine Chitosan or synthetic poly-ally-amine
hydrochloride (PAH) – FDA approved
NaCl-CaCl2 PAH
Chitosan

6. PCEP- CaCl2
MPs
PCEP- PAH
MPs
PCEP- CHIT
MPs
PCEP particle uptake by DCsPCEP particle uptake by DCs
Garlapati et al manuscript in submission

7. Garlapati et al manuscript in submission

9. RSV MARKET POTENTIAL

11. Discovery Pre-Clinical Phase-I Phase-II Phase-III
2020
MEDI-559
MEDI-534 (PIV3)
Novavax VLP F & G
PEV4 (virosome)
Protollin- RSV
RSV287
Nanobio RSV
Sendai Virus vector
Alpha Virus vector
Virosomes P3CSK4
rRSVΔG
DNA electoporation
Adenovirus vector

12. 0 21 44 48 50
Immunize Boost Challenge
Kill 1
Kill 2
Spleens - ELISPOT
Lung-Fragment cultures
BAL
Groups
1.Saline/RSV challenge
2.Ag IN
3.Ag Triple Combo SOL IN
4.Ag Triple Combo MP IN
5.Ag IM
6.Ag Triple Combo SOL IM
7.Ag Triple Combo MP IM
Triple Combo formulation with ΔF protein
Viral Shedding
Lung Cytokines

13. RSV-specific humoral responses in serum.

14. Serum Virus Neutralization TitersVirus Titers

15. Lung Fragment Culture

16. Cytokine ELISPOT of Splenocytes

17. Cytokines in Lung Homogenates Post-RSV Challenge

18. Acknowledgements….Acknowledgements….
Nelson Eng
Gael Auray
Tadele Kiros
Rachelle Buchanan
Stacy Strom
Monika Polewicz
Aleksandra Gracia
Laura Latimer
Marlene Snider
Ravinder Garg
Volker Gerdts
Lorne Babiuk
Andrew Potter
Sylvia v.d. Hurk
Heather Wilson
George Mutwiri
Jim Holmstrom
Hugh Townsend
VIDO
Funding: - B. & M. Gates Foundation
- Canadian Institute of Health Research
- Krembil Foundation
- National Science and Engineering Research Council
- Saskatchewan Health Research Foundation
Animal Care
Barry
Walker
Sherry
Others…