Definitions of Rabies and Biological assay of rabies vaccine. Presented by R. BHANUTEJA Department of Pharmaceutical Analysis

1. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 1
Presented by
R. BHANUTEJA
(Reg. No: 20L81S0707)
Under the guidance of
Mr. Shakir bhasha, M. Pharm.
Professor of Pharmaceutical Analysis ,
Head of department – Diploma in pharmacy.
BIOLOGICALASSAY OF RABIES
VACCINE
A seminar as a part of curricular requirement for 1st year
M. Pharm 1st semester

2. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 2
1. Definitions.
2. Biological assay of rabies vaccine.
3. References.
Contents

3. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 3
Rabies :-
• Rabies is a zonotic disease which is caused by a lyssa virus belongs to the
family Rhabdoviridae.
Rabies vaccine :-
• Rabies vaccine is a suspension of suitable strain of fixed rabies virus grown in
suitable approved culture and inactivated by suitable method.
Bioassay :-
• The potency of rabies vaccine is determined by comparing the lethal cerebral
dose of rabies virus with the dose of standard preparation of rabies.
Definitions

4. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 4
Standard preparation :-
• Freeze dried preparation.
• The standard preparation is the 5th international standard established in
1987 containing 16 units or another suitable preparations.
Suggested methods :-
a) Test animals.
b) Standard challenge virus suspension.
c) Virus titre of the challenge virus.
d) Determination of potency of vaccine
Biological assay of rabies vaccine

5. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 5
• Albino mice weight about three to four weeks old, 11g-15g weight.
• Distribute mice into Six groups of 16 animals, four groups of 10
animals.
• These above two groups are used for titration of LD50 challenge
suspension.
• Inject 0.03 ml per mice intracerebrally.
• 3 groups of 16 receive standard preparation and other 3 the vaccine
examined.
• 4 groups of 10 receive LD50 of the challenge suspension.
Test animals

6. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 6
Standard challenge virus suspension is prepared by injecting
Intracerebrally 0.03ml of 10 fold dilution standard strain horse serum to test animals.
Showing characteristic symptoms of encephalitis are sacrificed, harvest the brain
Wash it with saline solutions to remove blood clots
Homogenized brain with 10% digested casein hydrosylate (PH-7.2)
centrifuge and separate liquid is distributed into ampoules and stored at 2-80
Standard challenge virus suspension

7. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 7
Prepare 10 fold dilution of standard challenge virus suspension
0.03 ml inject intracerebrally to a group of 10 mice
observe for 14 days & count the mice surviving in each group
calculate the virus titre of standard challenge virus suspension by statistical method.
Determination of challenge virus

8. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 8
prepare 3-5 fold serial dilution of standard and test solution of vaccine
inject 0.03 ml intra peritoneally & after 7 days prepare same solution and inject
Both standard and test should be prepared in such a way
After 7 days inject 0.03ml standard virus suspension to vaccinated mice
Observe it for 14 days and calculate its potency by statistical method
Determination of potency of vaccine

9. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721 9
• Rishi Ram parajul, Rabies vaccine, The Indian pharmacopoeia
commission, Ghaziabad, vaccine: General Requirements: 1355-1356.
• Indian pharmacopoeia1996, volume 2, pg no :- 657-658.
References

10. RIPER
AUTONOMOUS
NAAC &
NBA (UG)
SIRO- DSIR
Raghavendra Institute of Pharmaceutical Education and Research - Autonomous
K.R.Palli Cross, Chiyyedu, Anantapuramu, A. P- 515721
THANK YOU