YEAST ARTIFICIAL CHROMOSOME - BASIC NOTES

1. YEAST ARTIFICIAL
CHROMOSOME
KAVIYALAKSHMI M
ASSISTANT PROFESSOR

2. INTRODUCTION
• Yeast artificial chromosomes (YACs) are genetically
engineered chromosomes derived from the DNA of the
yeast.
• It is a human-engineered DNA molecule used to
clone DNA sequences in yeast cells.
• They are the products of a recombinant DNA cloning
methodology to isolate and propagate very large
segments of DNA in a yeast host.

3. •Originated from Eukaryotic cells.
•They can be maintained in both yeast cells and
bacterial cells.
•Also called Shuttle Vector.
INTRODUCTION

4. STRUCTURE
COMPONENTS:
1. CENTROMERE
2. TELOMERE
3. SELECTABLE MARKER GENE
4. ORI GENE
5. ARS GENE
SIZE – 200-500 kb
HOLDING CAPACITY – 200 – 2000kb

5. CONSTRUCTION
1
• SELECTION OF YAC
2
• DIGESTION BY Bam H III
3
• LINEAR YAC FORMATION
4
• DIGESTION BY Eco R I
5
• LIGATION OF DESIRED DNA
6
• TRANSFORMATION OF rYAC
7
• SCREENING PROCESS

6. CONCLUSION
• ADVANTAGES
1. accommodate large
DNA fragments
2. Replicate like natural
chromosomes
3. Used for gene therapy
4. Large cloning capacity
• DISADVANTAGES
1. Instability
2. Complexity
3. Less stable than BAC
4. Low yield
5. Cost