Research Methodology In Medical Research. This presentation gives an comprehensive overview of research methodology in biomedical research also includes different types of medical research and ethics in medical research.
inform consent form before participate in clinical trials.for purpose of understanding the nature of research,risk,benefits,and decision about participation
Retrospective vs Prospective Study: Advantages, Types and Differences.
https://www.cognibrain.com/retrospective-vs-prospective-study-advantages-types-and-differences/
Study designs, Epidemiological study design, Types of studiesDr Lipilekha Patnaik
Study design, Epidemiological study designA study design is a specific plan or protocol
for conducting the study, which allows the investigator to translate the conceptual hypothesis into an operational one.
Research Methodology In Medical Research. This presentation gives an comprehensive overview of research methodology in biomedical research also includes different types of medical research and ethics in medical research.
inform consent form before participate in clinical trials.for purpose of understanding the nature of research,risk,benefits,and decision about participation
Retrospective vs Prospective Study: Advantages, Types and Differences.
https://www.cognibrain.com/retrospective-vs-prospective-study-advantages-types-and-differences/
Study designs, Epidemiological study design, Types of studiesDr Lipilekha Patnaik
Study design, Epidemiological study designA study design is a specific plan or protocol
for conducting the study, which allows the investigator to translate the conceptual hypothesis into an operational one.
A document that provides an unbiased and comprehensive synthesis
of relevant studies and research.
Characteristics of a Systematic Review
Purposes of a systematic review
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
Title: Sense of Smell
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the primary categories of smells and the concept of odor blindness.
Explain the structure and location of the olfactory membrane and mucosa, including the types and roles of cells involved in olfaction.
Describe the pathway and mechanisms of olfactory signal transmission from the olfactory receptors to the brain.
Illustrate the biochemical cascade triggered by odorant binding to olfactory receptors, including the role of G-proteins and second messengers in generating an action potential.
Identify different types of olfactory disorders such as anosmia, hyposmia, hyperosmia, and dysosmia, including their potential causes.
Key Topics:
Olfactory Genes:
3% of the human genome accounts for olfactory genes.
400 genes for odorant receptors.
Olfactory Membrane:
Located in the superior part of the nasal cavity.
Medially: Folds downward along the superior septum.
Laterally: Folds over the superior turbinate and upper surface of the middle turbinate.
Total surface area: 5-10 square centimeters.
Olfactory Mucosa:
Olfactory Cells: Bipolar nerve cells derived from the CNS (100 million), with 4-25 olfactory cilia per cell.
Sustentacular Cells: Produce mucus and maintain ionic and molecular environment.
Basal Cells: Replace worn-out olfactory cells with an average lifespan of 1-2 months.
Bowman’s Gland: Secretes mucus.
Stimulation of Olfactory Cells:
Odorant dissolves in mucus and attaches to receptors on olfactory cilia.
Involves a cascade effect through G-proteins and second messengers, leading to depolarization and action potential generation in the olfactory nerve.
Quality of a Good Odorant:
Small (3-20 Carbon atoms), volatile, water-soluble, and lipid-soluble.
Facilitated by odorant-binding proteins in mucus.
Membrane Potential and Action Potential:
Resting membrane potential: -55mV.
Action potential frequency in the olfactory nerve increases with odorant strength.
Adaptation Towards the Sense of Smell:
Rapid adaptation within the first second, with further slow adaptation.
Psychological adaptation greater than receptor adaptation, involving feedback inhibition from the central nervous system.
Primary Sensations of Smell:
Camphoraceous, Musky, Floral, Pepperminty, Ethereal, Pungent, Putrid.
Odor Detection Threshold:
Examples: Hydrogen sulfide (0.0005 ppm), Methyl-mercaptan (0.002 ppm).
Some toxic substances are odorless at lethal concentrations.
Characteristics of Smell:
Odor blindness for single substances due to lack of appropriate receptor protein.
Behavioral and emotional influences of smell.
Transmission of Olfactory Signals:
From olfactory cells to glomeruli in the olfactory bulb, involving lateral inhibition.
Primitive, less old, and new olfactory systems with different path
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Ve...kevinkariuki227
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
TEST BANK for Operations Management, 14th Edition by William J. Stevenson, Verified Chapters 1 - 19, Complete Newest Version.pdf
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdf
Research protocol writting
1. L-9:Writing research protocol
Presented by: Dr.Dawal S. Salve.
PG student MD(Community medicine)
Activity guide: Dr. N.R.Aswar MD(PSM).
Associate Professor.
DR.S.C.G.M.C.Nanded.
Date:04/10/12
2. L-9:Writing research protocol
Components of a research protocol
Title and abstract
Introduction, aims and objective
Context-review of relevant literature
Methodology-research question, study design, description of
methodology, plan of analysis
References- Citation, Citing styles, citing journals, books, internet
references.
3. What is research?
• Research is the systematic collection, analysis
and interpretation of data to answer a certain
question or solve a problem
4. Why a protocol?
To clarify the
research
question
To compile
existing
knowledge
To formulate a
hypothesis and
objectives
To decide about
a study design
To clarify
ethical
considerations
To apply for
funding
To have a
guideline and
tool for the
research team
5. How to judge well-thought out and well-written
protocol?
Is it adequate to answer the research
question(s)and achieve the study
objective?
Is it feasible in the particular set-up for
the study?
Does it provide enough detail that can
allow another investigator to do the study
and arrive at comparable conclusions?
6. Content of the protocol
• Project title
• Project summary
• Project description:
− Rationale
− Objectives
− Methodology
− Data management and analysis
• Ethical considerations
• Gender issues
• References
7. Project Title
• Accurate, short, concise
• Descriptive: should make the main objective
clear, should mention the target population
• Key words: should contain key words for
referencing
– i.e. „Anemia in hiv infected ANC“
– Better: „prevalence of anemia among
HIV infected ANC attending ICTC centre Nanded.“
8. • Concise, one page (about 300 words)
• Stands on its own – no reference to protocol
content
• Summarizes central elements (rationale,
methodology, populations, time frame, expected
outcomes)
9. Statement of the problem
• Why …is the research needed?
• What …is the relevance of the results?
Logical flow of statements:
Magnitude, frequency, and distribution: Affected geographical areas and
population groups. Ethnic and gender considerations.
Probable causes of the problem: What is the current knowledge of the
problem and its causes? Is there consensus? Is there controversy? Is there
conclusive evidence?
Possible solutions: In what ways have solutions to the problem been
attempted? What has been proposed? What are the results?
Unanswered questions: What remains to be answered? What areas have not
been possible to understand, determine, verify, or test?
10. Statement of the problem
Literature review
• Prevents duplication of work, which has been
done
• Clarifies, what others have found addressing the
problem
• Familiarizes with potential methodologies and
methodological errors
• Should convince, that the research is needed!
11. Justification of research
Should be a convincing statement for the need to do
this research
• How does the research relate to the priorities of
the region and the country?
• What knowledge and information will be
obtained?
• What is the ultimate purpose that the knowledge
obtained from the study will serve?
• How will the results be disseminated?
• How will the results be used, and who will be the
beneficiaries?
12. Research hypothesis
Independent
variable
• Describes the relationship between INDEPENDENT
variables (risk factors, predisposing factors) and
DEPENDENT variables (outcome)
• Determines the type of data to be collected and the
type of analysis to be conducted
Dependent
variable
Intervention,
Observation, Control
variable
Make clear
statements!
14. Research objectives
Define a GENERAL OBJECTIVE and derive SPECIFIC
OBJECTIVES
The formulation of objectives will help to:
• Focus the study (narrowing it down to essentials)
• Avoid the collection of data which are not strictly
necessary for understanding and solving the
problem you have identified
• Organize the study in clearly defined parts or phases
15. Research objectives
Objectives should be:
• Logical and coherent
• Feasible
• Realistic, considering local conditions
• Defined in operational terms that can be
measured
• Phrased to clearly meet the purpose of the study
(relevant)
Objectives should be stated in action verbs that illustrate their purpose:
i.e. “To determine…, To compare..., To verify..., To calculate...,To reduce..., To describe...,
etc.
16. Methodology
Important aspect of the protocol
• Assures, that the hypothesis will be proved or
disproved, using the right tools
• Presents a detailed strategy, how the objectives
are achieved
Consider
• operational definitions
• study design
• definition of variables
• sample size
• ethical aspects
17. Methodology
Study design
• The decision on study design needs to be based
on ethics, logistic consideration, economic
aspects and scientific thoroughness.
• The validity of the results including potential bias
and confounding and the generalizability of the
results needs to be a prime concern defining the
study design.
• A causal relationship between exposure and
outcome can be best established in a Clinical
trial
– > Cohort study > Case - control study > Cross-sectional
study > Ecological study
18. Methodology
Study design
Interventional study Observational study
Clinical trial Cohort study
Cross-sectional study
Case – control study
Ecological study
20. Methodology
Measurement of observations
• Describe how, when and where the observations
are made? Describe instruments used!
– Questionaire (attach to the protocol)
– Type of interview (describe structure of the interview)
– Laboratory test (refer to literature or personal
knowledge, if established test, or describe in detail, if
not established)
– Clinical examinations (describe gadget/procedure)
Describe all instruments or refer to literature if tools are established.
(for intervention studies or drug trials refer to specific literature/regulations)
21. Methodology
Subjects/ participants
Depending on the type of study, answer the
following questions:
•What are the criteria for inclusion or selection?
•What are the criteria for exclusion?
•In intervention studies, how will subjects be
allocated to index and comparison groups
(Randomization procedure)?
•What are the criteria for discontinuation?
22. Methodology
Data quality control
• Describe measures of data quality control:
– Double data entry
– Consistancy checking
– Data monitor
– Verfication procedures of source data
– Interrater variability measurements
23. Methodology
Sample size
• Sample size calculation is recommeded for
economical and ethical reasons
• Simple statistical packages in the internet
• Level of error, power and expected impact
of exposure on outcome have to be set
24. Methodology
(Intervention)
If applicable, describe:
• Who will be responsible for the
intervention?
• Where will it take place?
• What activities will be performed, and
with what frequency and intensity?
25. Ethical considerations
One of the most important aspects of biomedical
research!
Ask local ethics committee for advice!
Important aspects to consider:
• Data safety / confidentiality
• Any possibly harm/ side effects/ consequences
• Right of discontinuation at any time
• Role of data safety and monitoring board (in trials)
• Alternative treatments/ approaches (in trials)
• Incentives/ Rewards
26. Ethical considerations
Informed consent
Outline how, when and where the patient will be
consented!
Information form should contain:
• Justification for research
• Responsibilities (Who)
• Outline of study
• Risks
• Insurance
• Confidentiality (legal framework)
• Voluntary participation
• A separate consent form is required!
Simple language!
Patients mother tongue!
27. Ethical considerations
Ethics checklist
• Should answer potential questions regarding the
ethics
• Should discuss pros and cons of research design,
selection of subject, measurement and outcome
assessment.
• Should discuss the advantages and
disadvantages of the subject or communities
involved
• Should discuss physical, social and psychological
implications of the research
• Should discuss confidentiality
28. Data management and analysis
Based on objectives consider:
• Coding for variables/ type of variables
• Analysis plan depending on type of variables
• Statistical tests implicated
• (Style of presentation i.e. tables, graphs)
• Data safety and storage
29. References/Bibliography
• Use of standard referencing system:
– Harvard style
• Name and publication year in text
• Alphabetical bibliography
– Vancouver style
• Numbered references
• Continous referencing in text
• Make use of software
– Reference Manager
– Endnote software
See also „A Pratical Guide for Health Researchers“
31. What is the Harvard System?
• Developed in the USA
• Most common system in use
internationally
• A flexible, simple, clear system both for
author and reader
• References are listed alphabetically in the
bibliography and cited in the body of the
text so no footnotes or chapter references
needed
32. What is the Vancouver Referencing Style?
It is a uniform set of requirements for
bibliographic references.
It is a "numbered" style.
It follows rules established by the International
Committee of Medical Journal Editors.
It is also known as: Uniform Requirements for
Manuscripts submitted to Biomedical Journals.
33. How to do in-text citations
A number is assigned to each reference as it is cited
- in brackets [ ] ( ) or superscript.1
If the source is referred to again, the same number is
used.
Example:...as one author has put it "the darkest days
were still ahead" [1]: which is well documented in the
literature. [2-5] This proves that "the darkest days were
still ahead". [1]
34. How to create a Reference List
Reference list should appear at the end of the assignment / report.
Entries are listed numerically and in the same order that they were
cited in the text.
Example:
1. Baker PN, editor. Obstetrics by ten teachers. 18th ed. London:
Hodder Arnold; 2006.
2. Delbridge ML, Harry JL, Toder R. A human candidate
spermatogenesis gene, RBM1, is conserved and amplified on
marsupial Y chromosome. Nat Genet. 1997; 15: 131-136.
36. Budget
The budget should be broken down by:
• Items
– Personnel
– Consumables, equipment, supplies,
communication, funds for patients, data
processing
• Budget justification
– Jusitify the use of each item, considering the
workplan of the study
37. Annexes
• Case Record Forms (CRFs)
• Questionaires
• Patient information form (in required
languages)
• Consent form (in required languages)
• CV of investigators
40. Methodology
• Research design:
• Research subjects or participants:
• Interventions: drugs or devices.
• Observations:
• Sample size:
41. Ethical considerations
• Approval by ethics review committees:
• Informed decision-making:
Consent form:
A) A statement describing the study and the nature of the subject’s
involvement in it; and
b) A certificate of consent attesting to the subject’s consent.
• Ethics checklist
.
42. Questionnaire Design
•Review of data source and data collection
•Instruments for data collection
•Questionnaire as a tool for data collection tool-types, strengths and
limitations
•Principles of questionnaire design-purpose, type of information, wording
the questions, response rate and rating scales
•Questionnaire Administration-prevalidation,response rate, reliability and
validity
Editor's Notes
Formation of the topic Hypothesis
Conceptual definitions
Operational definition
Gathering of data
Analysis of data
Test,
revising of hypothesis
Conclusion,
iteration if necessary
Study design – causal relationship between exposure and outcome is relevant
Ethical considerations - in new TB drugs I still need to cure the patients
Order is not relevant
Methodological errors – effectiveness of DOTS in Chochrane – did not look at resistance, not Rifa – resistance – missed important outcome.
After statement of the primary objective, secondary objectives may be mentioned. Young investigators are advised to resist the temptation to put too many objectives or
over-ambitious objectives that cannot be adequately achieved by the implementation of
the protocol.
Good example for the superiority of trial – HRT from woman – later WHI trial......
Simple language mother tongue!
For studies in humans (or involving human biological materials), the protocol must
be approved by the local, institutional or equivalent ethics committee and/or national
ethics committee. For animal studies approval is required from the animal welfare committee of the
institute or its equivalent. If no such committee exists, a statement signed by the principal
investigator(s) should indicate that the research will be carried out in accordance with
the International Guiding Principles for Biomedical Research involving Animals