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Vitamins
1. Kurdistan Region- Iraq
Salahaddin University–erbil (SUE)
College Of Science
Department Of Chemistry
Analysis of
Prepared by:
Kosary Yasin Ahmed
Supervised by:
Msc. Narmin sahdula
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2. content
How I searched in labrary
Introduction
Difinition of vitamins
Classification of vitamins
Food source of vitamins
Health benefit of vitamins
Vitamin analysis method
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6. Introduction
The term “vitamin” (“vital amine”) was coined by the
Polish biochemist Casimir Funk 100 years ago, and the
discovery of vitamins was concentrated mainly in the
Hrst half of the 20th century. During that time, the
focus of both animal and human nutrition research
was on symptoms of vitamin deficiency and hence
associated diseases.
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7. Vitamin analysis in food
Vitamin analysis in food has a variety of purposes; it is used to provide
quality assurance for supplemented products; to study changes in vitamin
content attributable to food processing, packing, and storage;
to provide data for food composition tables; and to check compliance with
contract specifications and nutrient labeling regulation
9. An organic chemical compound (or related set of compounds) is
called a vitamin when the organism cannot synthesize the
compound in sufficient quantities, and it must be obtained
through the diet.
What is vitamins?
Vitamin vita (life)-amine
A vitamin is an organic compound and a vital nutrient that an
organism requires in limited amounts.
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11. 1- Fat soluble vitamins 11
01
02
03
04
Vitamin K is a group of structurally similar, fat-soluble vitamins the human body
requires for complete synthesis of certain proteins that are prerequisites for blood
coagulation and which the body also needs for controlling binding of calcium in
bones and other tissues
Vitamin A is a group of unsaturated nutritional organic compounds that includes
retinol, retinal, retinoic acid, and several provitamin A carotenoids (most notably
beta-carotene).Vitamin A has multiple functions: it is important for growth and
development, for the maintenance of the immune system and good vision.
Vitamin D is a fat-soluble vitamin that is naturally present in very few foods,
added to others, and available as a dietary supplement. It is also produced
endogenously when ultraviolet rays from sunlight strike the skin and trigger
vitamin D synthesis.
Vitamin E refers to two groups of compounds, the tocopherols and the
tocotrienols, and all forms of the vitamin contain two parts, a “head” (the
chromanol part) and a “tail” (phytyl group) as shown in Figures l
The vitamins generally classified as the fat soluble vitamins are vitamins A, D, E and K
12. 2- Water soluble vitamins
A vitamin that can dissolve in water. Water-soluble
vitamins are carried to the body's tissues but are
not stored in the body. They are found in plant and
animal foods or dietary supplements and must be
taken in daily. Vitamin C and members of the
vitamin B complex are water-soluble.
Water
soluble
vitamin
01
Vitamin C
02
Niacin
03
Thiamin
04
Riboflavin
05
Vitamin B12
06
Pantothenic
acid
07
Biotin
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14. Sourceofvitamins
Vitamins Food source
Vitamin B1 (Thiamin)
Whole grains, enriched grains and
Liver
Vitamin B2 (Riboflavin)
Soybeans, meat liver eggs, Milk,
cheese and yogurt
Vitamin B3 (Niacin)
Mushrooms, Peanut butter, meat, fish,
poultry
Biotin
Sweet potatoes ,Nonfat milk, yogurt
Peanuts, almonds,
Vitamin C
Citrus fruits such as oranges,
grapefruits and their juices,
Vitamin A Liver, some fish , Milk, cheese
Vitamin D
Milk, fortified soy and rice, Some fish,
eggs, organ meats,
Vitamin E
Vegetable oils, Avocados, leafy green
vegetables
Vitamin K
Broccoli, soybeans, dark green leafy
vegetables such as kale
Folate
Asparagus, Brussels sprouts, beets,
broccoli, corn, green
Vitamin B6 (Pyridoxin) Potatoes, bananas
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16. Vitamin Analysis Method 16
Immunoassays (ELISA, RIA, FIA)
Colorimetric and Spectrophotometric assays
Fluorometric assay & Chemiluminescence assay
measure vitamins directly and give a quantitative measurement. These are recommended for use
particularly for clinically useful vitamins.
However these are expensive and not every laboratory can provide these assays. These methods
include:
Amperometric assay
HPLC (reference method)