1. The document discusses sources of variation in organisms, including both genetic factors like gene mutations and chromosomal mutations, as well as environmental factors like nutrition, temperature, and light intensity. 2. Variation can be continuous, with a range of values, or discontinuous, where an organism either has a characteristic or does not. Gene mutations like sickle-cell anemia or chromosomal mutations like Down's syndrome can cause variation. 3. An example is given of industrial pollution selecting for dark-colored moths that blended in better with the soot-covered trees, making light-colored moths more vulnerable to predation until environmental regulations reduced pollution.