chemicals required for tissue culture

2. Chemical requirements for
plant tissue culture

3. Chemical requirement
Chemicals in plant tissue culture are used for
Supply of minerals
Nutrients
And chemical conditions needed for plant
growth

4. Antimicrobial
Antibiotics
Antifungal
Antibiotics in plant tissue culture are
recommended for suppressing the growth of
bacteria, fungus and other microbes.

5. Biochemical
Amino acids
General chemicals
Organic acids
Organic supplements
vitamins

6. Carbohydrates
Chromogenic substrates
Disinfectants
Indicators , stains and dyes

7. Enzymes
Gelling agents
Miscellaneous chemicals
Plant growth regulators
Steroids/ sterols- Campesterol, Brassinolide

8. Plant growth regulators
Plant growth regulators are chemicals that
influence the growth and differentiation of
plant tissue.
 Auxin, cytokinin, GA3, ABA, ethylene are
essential plant growth regulators or
hormones.

10. Miscellaneous Chemicals
Some of the chemicals in plant tissue culture
are being used for specific purpose.
 Colchicine is used for mutagenic purpose
while PEG is used in plant tissue culture to
induce the stress.

11. Gelling Agent
Gelling Agent are used in plant tissue to
provide a solid and semi solid matrix to
support the plant parts.
Agar, Clarigel

12. Gelling agents enable the plants to be placed in
precise contact with the medium.

13. Enzymes
Enzymes are biological catalysts.
They increase the rate of reactions, allowing
the chemical reactions that make life possible
to take place at normal temperatures.
Cellulase, Pectinase

14. Dyes, Indicators and Stains
Dyes, stains, and indicators are all integral
tools in the laboratory
Dyes in microscopy- cell and tissue
components in plant material have to be
visualized.
Indicators-for measuring end point of
titration or setting pH of solutions.

15. Disinfectants
Used for decontamination
Hydrogen peroxide
Calcium hypochlorite
Mercuric chloride

16. Chromogenic Substrates
Chromogenic substrates are the precursors
that may be transformed by biological
mechanism in to colord compounds.
 Used knowing the transformed cells or
plants.
5-Bromo-3-indolyl-ß-D-galactopyranoside

17. Carbohydrates (carbon source)
Carbon source
Building blocks for macromolecules.
 Therefore various sugars used in the
medium.
Galactose, glucose, mannose, ribose