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Presented by:-
Mohammad Nijam Alvi
Enroll no. B1373/14
College of biotechnology DUVASU Mathura
 What is callus culture
 Media
 Constituents of Media
 Process of callus culture
 Advantages
 Disadvantages
 Application
 Callus tissue means an unorganised
proliferative mass of cells produced from
isolated plant cells, tissues or organs when
grown aseptically on artificial nutrient
medium in glass vials under controlled
experimental conditions.
 MS medium:- Murashige and Skoog (MS) originally formulated a
medium to induce and regeneration of plants in cultured tissues.
 B5 medium:-B5 medium was originally designed for cell
suspension and callus cultures.
 N6 medium:- Chu formulated this medium and it is used for
cereal anther culture, besides other tissue cultures.
Callus Culture: Media, Process, Uses & Benefits

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Callus Culture: Media, Process, Uses & Benefits

  • 1. Presented by:- Mohammad Nijam Alvi Enroll no. B1373/14 College of biotechnology DUVASU Mathura
  • 2.  What is callus culture  Media  Constituents of Media  Process of callus culture  Advantages  Disadvantages  Application
  • 3.  Callus tissue means an unorganised proliferative mass of cells produced from isolated plant cells, tissues or organs when grown aseptically on artificial nutrient medium in glass vials under controlled experimental conditions.
  • 4.  MS medium:- Murashige and Skoog (MS) originally formulated a medium to induce and regeneration of plants in cultured tissues.  B5 medium:-B5 medium was originally designed for cell suspension and callus cultures.  N6 medium:- Chu formulated this medium and it is used for cereal anther culture, besides other tissue cultures.