This chapter discusses hypothesis testing for comparing means and variances between two populations or samples. It covers testing for the difference between two independent population means, two related (paired) population means, and two independent population variances. The key tests covered are the pooled variance t-test and separate variance t-test for independent samples, and the paired t-test for related samples. Examples are provided to demonstrate how to calculate the test statistic and conduct the hypothesis test to determine if the means or variances are significantly different.