This document describes protocols for diagnosing endo- and ectoparasites in laboratory rats and mice. It outlines methods for examining feces through perianal tape tests, fecal flotation, and centrifugation to detect endoparasite eggs, larvae, cysts or worms. Autopsies are described to examine intestines and detect helminths. Ectoparasites are examined through fur plucking, skin scraping, and pelage examination. Representative results show the parasites detectable by each method and appropriate detection solutions. Limitations include some methods requiring euthanasia and PCR not being available for all protozoans.
The document discusses guidelines for collecting wound swab specimens. It states that wound swabs are important samples sent to microbiology to identify bacteria and fungi causing infections. However, current practices for collecting and identifying specimens have deficiencies. The document then provides guidance on proper techniques for collecting representative wound swab samples, including cleaning the wound, using sterile swabs, sampling various areas, and promptly transporting swabs to the microbiology lab.
This document provides information on basic identification methods for human parasitology. It discusses various parasite identification techniques including microscopic examination of wet mounts, concentration methods for stool samples, and examination of other specimens like sputum, biopsies, and blood. The document outlines procedures for collecting and preparing stool, sputum, and blood samples for examination and describes examining samples microscopically to identify parasites, eggs, larvae, trophozoites or cysts of organisms like hookworm, tapeworm, whipworm, pinworm, Entamoeba, and Giardia. It also discusses concentrating techniques, normal values, artifacts, and use of the QBC method for diagnosing parasites in blood samples.
CONCENTRATIONS TECHNIQUES IN PARASITOLOGY PRESENTATION.pptxShreyayadav91
INTRODUCTION
Concentration procedure separate parasites from fecal debris and increase the chances of detecting parasitic organisms when these are in small numbers.
If number of organisms in stool specimen is low, examination of a direct wet mount may not detect parasites.
Thus, whenever possible, the stool should be concentrated.
Advantages
Maximizes the numbers of organisms detected which may be too scanty to be seen by direct microscopy alone. Worm eggs, larvae, and protozoan cysts may be recovered.
Disadvantages
Destroys trophozoite stages. Most concentration methods destroy trophozoites stages.
Concentration techniques can be classified as the floatation or sedimentation methods.
Floatation technique
Here solutions with higher specific gravity than the organisms to be floated so that the organisms rise to the top and debris sink to the bottom.
Principle
This technique involves suspending the specimen in a medium of greater density than that of the helminthic eggs and protozoan cysts.
Eggs and cysts float to the top and are collected by placing a glass slides on the surface of the meniscus at the top of the tube.
Floatation Methods includes:
Saturated salt solution technique
Zinc sulfate centrifugal floatation
Sugar floatation technique
Saturated salt solution technique
Procedure:
About half tea spoon (about 4 gm) of fresh stool or preserved stool in a flat bottomed container with 20 ml capacity.
Now, few drops of saturated salt solution (specific gravity 1.20) is added and stirred to make a fine emulsion.
More salt solution is added with stirring throughout to fill the container up to the brim, until a convex meniscus is formed.
A glass slide (3”*2”) is carefully laid on the top of the container so that the center is in contact with the fluid.
This preparation is allowed to stand for 20 minutes after which the glass slide is quickly lifted and examined under microscope after putting coverslip.
Zinc sulfate centrifugal floatation
Procedure
Make a fine suspension of about 1 g of feces in 10 m L of water and strain through gauze to remove coarse particles.
Collect the liquid in a small test tube and centrifuge for 1 minute at 2,500 revolutions per minute. Pour off the supernatant, add water, resuspend, and centrifuge in the same manner, repeating the process, till the supernatant is clear.
Pour off the clear supernatant, add a small quantity of zinc sulfate solution (specific gravity 1.18- 1.2) and resuspend the sediment well.
Add zinc sulfate solution to a little below the brim and centrifuge at 2,500 revolution per minute for 1 minute.
Take samples care fully from the surface, using a wire loop, transfer to slide and examine under the microscope. A drop of dilute iodine helps to bring out the protozoan cysts in a better way.
This technique is useful for protozoan cysts and eggs of nematodes and small tapeworms, but it does not detect unfertilized roundworm eggs, nematode larvae, and eggs of most trematodes and large tapeworms.
1. There are various routes for inoculating eggs with viruses, including the allantoic, chorio-allantoic, yolk sac, and amniotic routes. The allantoic route through the air sac is most common.
2. For the chorio-allantoic route, two small holes are made - one in the air sac and another in the bloodless chorio-allantoic membrane. Air is passed through the first hole to create an artificial air sac space, and then the vaccine is injected.
3. For the yolk sac route, a longer needle is used to inject the virus seed through the air sac and into the deeper yolk sac area.
Collection of fungal samples Lab manual Vamsi kumar
This document provides instructions for an experiment on collecting fungal samples from hair, nails, and skin. It details the necessary materials, pre-test procedures, and optimized techniques for collecting skin scrapings, nail cuttings/scrapings, and hair specimens. Negative cultures may occur if the condition is not fungal, improper collection techniques were used, or antifungal treatments were administered prior. Fungal cultures aim to confirm infections, determine the fungal species, and aid epidemiological investigations.
This document summarizes various laboratory techniques for diagnosing parasitic infections through direct examination of samples like urine, stool, sputum, biopsy specimens, and aspirates, as well as indirect immunological methods and molecular biological techniques. Direct examination involves microscopic evaluation of samples for parasite eggs, larvae, cysts, trophozoites, or adult parasites, while concentration techniques help find parasites in low-density infections. Indirect methods detect antibodies to parasites, and molecular techniques like PCR can identify parasitic DNA in samples.
The document discusses guidelines for collecting wound swab specimens. It states that wound swabs are important samples sent to microbiology to identify bacteria and fungi causing infections. However, current practices for collecting and identifying specimens have deficiencies. The document then provides guidance on proper techniques for collecting representative wound swab samples, including cleaning the wound, using sterile swabs, sampling various areas, and promptly transporting swabs to the microbiology lab.
This document provides information on basic identification methods for human parasitology. It discusses various parasite identification techniques including microscopic examination of wet mounts, concentration methods for stool samples, and examination of other specimens like sputum, biopsies, and blood. The document outlines procedures for collecting and preparing stool, sputum, and blood samples for examination and describes examining samples microscopically to identify parasites, eggs, larvae, trophozoites or cysts of organisms like hookworm, tapeworm, whipworm, pinworm, Entamoeba, and Giardia. It also discusses concentrating techniques, normal values, artifacts, and use of the QBC method for diagnosing parasites in blood samples.
CONCENTRATIONS TECHNIQUES IN PARASITOLOGY PRESENTATION.pptxShreyayadav91
INTRODUCTION
Concentration procedure separate parasites from fecal debris and increase the chances of detecting parasitic organisms when these are in small numbers.
If number of organisms in stool specimen is low, examination of a direct wet mount may not detect parasites.
Thus, whenever possible, the stool should be concentrated.
Advantages
Maximizes the numbers of organisms detected which may be too scanty to be seen by direct microscopy alone. Worm eggs, larvae, and protozoan cysts may be recovered.
Disadvantages
Destroys trophozoite stages. Most concentration methods destroy trophozoites stages.
Concentration techniques can be classified as the floatation or sedimentation methods.
Floatation technique
Here solutions with higher specific gravity than the organisms to be floated so that the organisms rise to the top and debris sink to the bottom.
Principle
This technique involves suspending the specimen in a medium of greater density than that of the helminthic eggs and protozoan cysts.
Eggs and cysts float to the top and are collected by placing a glass slides on the surface of the meniscus at the top of the tube.
Floatation Methods includes:
Saturated salt solution technique
Zinc sulfate centrifugal floatation
Sugar floatation technique
Saturated salt solution technique
Procedure:
About half tea spoon (about 4 gm) of fresh stool or preserved stool in a flat bottomed container with 20 ml capacity.
Now, few drops of saturated salt solution (specific gravity 1.20) is added and stirred to make a fine emulsion.
More salt solution is added with stirring throughout to fill the container up to the brim, until a convex meniscus is formed.
A glass slide (3”*2”) is carefully laid on the top of the container so that the center is in contact with the fluid.
This preparation is allowed to stand for 20 minutes after which the glass slide is quickly lifted and examined under microscope after putting coverslip.
Zinc sulfate centrifugal floatation
Procedure
Make a fine suspension of about 1 g of feces in 10 m L of water and strain through gauze to remove coarse particles.
Collect the liquid in a small test tube and centrifuge for 1 minute at 2,500 revolutions per minute. Pour off the supernatant, add water, resuspend, and centrifuge in the same manner, repeating the process, till the supernatant is clear.
Pour off the clear supernatant, add a small quantity of zinc sulfate solution (specific gravity 1.18- 1.2) and resuspend the sediment well.
Add zinc sulfate solution to a little below the brim and centrifuge at 2,500 revolution per minute for 1 minute.
Take samples care fully from the surface, using a wire loop, transfer to slide and examine under the microscope. A drop of dilute iodine helps to bring out the protozoan cysts in a better way.
This technique is useful for protozoan cysts and eggs of nematodes and small tapeworms, but it does not detect unfertilized roundworm eggs, nematode larvae, and eggs of most trematodes and large tapeworms.
1. There are various routes for inoculating eggs with viruses, including the allantoic, chorio-allantoic, yolk sac, and amniotic routes. The allantoic route through the air sac is most common.
2. For the chorio-allantoic route, two small holes are made - one in the air sac and another in the bloodless chorio-allantoic membrane. Air is passed through the first hole to create an artificial air sac space, and then the vaccine is injected.
3. For the yolk sac route, a longer needle is used to inject the virus seed through the air sac and into the deeper yolk sac area.
Collection of fungal samples Lab manual Vamsi kumar
This document provides instructions for an experiment on collecting fungal samples from hair, nails, and skin. It details the necessary materials, pre-test procedures, and optimized techniques for collecting skin scrapings, nail cuttings/scrapings, and hair specimens. Negative cultures may occur if the condition is not fungal, improper collection techniques were used, or antifungal treatments were administered prior. Fungal cultures aim to confirm infections, determine the fungal species, and aid epidemiological investigations.
This document summarizes various laboratory techniques for diagnosing parasitic infections through direct examination of samples like urine, stool, sputum, biopsy specimens, and aspirates, as well as indirect immunological methods and molecular biological techniques. Direct examination involves microscopic evaluation of samples for parasite eggs, larvae, cysts, trophozoites, or adult parasites, while concentration techniques help find parasites in low-density infections. Indirect methods detect antibodies to parasites, and molecular techniques like PCR can identify parasitic DNA in samples.
Direct microscopic examination of clinical specimens can provide a presumptive diagnosis of fungal infection by revealing the presence of fungal elements. Different stains and techniques are used to visualize fungi depending on the suspected infection. KOH wet mounts are useful for superficial mycoses while GMS, H&E and fluorescent antibody stains aid in diagnosis of deep mycoses from tissue biopsies and body fluids. Proper specimen collection and rapid microscopic evaluation can help initiate appropriate antifungal treatment.
This document describes procedures for examining fecal samples to detect parasite eggs under a microscope. Several methods are discussed, including direct smear, McMaster technique, simple floatation, and sedimentation. Using these methods on samples from sheep, cattle, and dogs, several parasite eggs were observed, including Ancylostoma caninum, Toxocara canis, Fasciola eggs, Trichuris eggs, and Dipylidium caninum eggs enclosed in a capsule. The document concludes that multiple examination methods are needed to thoroughly detect parasites at different infection levels in fecal samples.
Fecal examination is commonly used to diagnose parasitic infections in animals. The process involves collecting a fresh fecal sample, preparing it using flotation or centrifugation with a flotation medium, and examining it under a microscope. Centrifugation speeds up the process by forcing heavier materials to the bottom and lighter parasite eggs to the top for easier identification. Examination of properly collected and prepared fecal samples can reveal evidence of parasitic infections and provide a diagnosis.
This ppt describes on the 5 tenets/principles, required for performing a fecal examination for parasitic eggs, larva, cyst observation required for arriving at a proper GI diagnosis.
This document discusses techniques for quantitatively estimating worm burden by calculating the number of eggs present in a stool sample. Specifically, it describes:
1) The Kato-Katz technique which involves pressing stool between a template and slide to obtain a standardized sample for egg counting under a microscope. The number of eggs observed can be used to estimate the total number of worms.
2) The Stoll technique where stool is suspended in sodium hydroxide solution and a subsample is examined. Egg counts are corrected based on stool consistency to estimate eggs per gram of formed stool.
3) The Beaver technique involves using a light meter to count eggs sandwiched between a slide and coverslip, but no details of
Microbes isolation from different environmentsMicrobiology
The document describes methods for isolating and identifying microbes from various samples. It discusses taking soil, water, food, slag and other samples and performing serial dilutions to isolate colonies. It explains incubating the samples and performing sub-culturing and biochemical tests to identify the bacterial species, including using mannitol salt agar to identify Staphylococcus aureus which turns the agar yellow. Coagulase tests are also described to differentiate S. aureus from other staphylococci.
Collection and transport of ear dischargesabeer-babeker
This document provides guidance on collecting and transporting ear discharge specimens for microbiological examination. Key points include:
1. Collect discharge on a sterile cotton swab and place in transport medium within 6 hours of collection.
2. Examine specimens microscopically with Gram stain and culture on blood agar, MacConkey agar, and thioglycollate broth. Additional media may be used depending on whether a bacterial or fungal infection is suspected.
3. Common pathogens causing ear infections include Streptococcus pneumoniae, Streptococcus pyogenes, Haemophilus influenzae, Pseudomonas aeruginosa, and fungi such as Candida species.
The document provides guidelines for virus isolation techniques using embryonated eggs, outlining various inoculation routes including allantoic, amniotic, chorioallantoic membrane and discussing advantages of different routes for isolating specific viruses. It describes the process of candling eggs to check embryo viability and mark inoculation sites prior to introducing viral samples via needle injection into selected areas. Proper egg storage, cleaning, and incubator maintenance are also covered.
This document discusses cytology of the urinary tract. It describes three methods for collecting urine specimens: voided urine, catheter specimens, and bladder washings. Voided urine is the simplest but cells may degrade over time. Catheter specimens avoid contamination but can damage cells. Bladder washings provide high cellularity and preservation through saline irrigation. Specimens can be prepared via several methods including cytocentrifugation and direct smears. Normal urinary tract cytology shows a range of superficial and deep urothelial cell morphologies depending on collection method and location in the tract.
14.anaeli and nicolle. mycobacteriophages paper.anaelishockey
Two mycobacteriophages were isolated from a soil sample collected in Gurabo, Puerto Rico. Following enrichment and plaque purification, two distinct phages (named Shockage and Zombage) were isolated based on differences in morphology and protein band patterns. Shockage had a distinct protein profile while Zombage and another initially isolated phage had nearly identical protein bands, indicating they were the same phage. Further DNA sequencing would help fully characterize these newly isolated phages and determine if they represent unique viruses. Overall, this experiment demonstrated a method for isolating new mycobacteriophages from environmental samples.
Isolation of bacteria is a significant step in diagnosing and managing bacterial infections. It involves collecting specimens, preserving and transporting them to the lab, examining samples microscopically, and using various culture and non-culture methods to isolate bacteria. Culture methods include using solid or liquid media, and automated systems, to allow bacterial colonies to grow. Non-culture methods involve molecular techniques like PCR. Proper specimen handling and use of appropriate culture conditions and media allow isolation of pathogenic bacteria to enable treatment and control of infections.
1. Proper specimen collection and transport are critical for laboratory testing.
2. Guidelines for collection include using appropriate containers and transport media, minimizing contamination, and providing complete patient information.
3. Specimens should be transported within 2 hours of collection and delivered to the laboratory promptly using biohazard labeling and packaging.
This document provides information on various laboratory investigations for sexually transmitted infections (STIs). It describes procedures for dark field microscopy, Gram staining, Giemsa staining, wet mounts, KOH wet mounts, and acetic acid testing. These tests are used to detect organisms causing STIs like syphilis, gonorrhea, chancroid, trichomoniasis, and genital HPV infections. Specimen collection sites and examination of stained slides under the microscope are discussed for accurate diagnosis of common STIs.
The document provides information about the history and types of microscopy. It discusses early microscopes developed by Hans Lippershey, Hans Janssen, and Giovanni Faber in the 1590s. Antoni van Leeuwenhoek is noted for his discoveries of micro-organisms in the 1670s using microscopy. In 1893, August Köhler developed Köhler illumination, important for modern light microscopy. Light microscopes and electron microscopes are described. Microscopic examination of samples can provide diagnostic information for conditions like malaria and other infectious diseases.
Two mycobacteriophages were isolated from soil samples collected in Gurabo, Puerto Rico using protocols from the SEA-PHAGES resource guide. The soil samples were enriched to encourage phage growth. Plaques were purified through serial streaking and enrichment to isolate individual phage clones. The two isolated phages were subjected to medium and high titer assays to increase phage particle concentration. Future work will involve sequencing the DNA of the isolated phages.
This document provides guidance on proper specimen collection, transport, and processing for the laboratory diagnosis of fungal infections. Key steps include collecting specimens in a sterile manner, transporting them to the lab within 2 hours if possible, and processing them promptly through smear preparation, culture inoculation, and incubation. Maintaining proper documentation and selecting purulent material can help maximize diagnostic sensitivity. Adhering closely to collection and handling protocols helps ensure optimal recovery of fungi in the laboratory.
Egg inoculation by Chinithung ngullie (2)Cyrus Ngullie
This presentation has been made easy to make understand to the students by emphasizing more on the visual than the words.I hope it is helpful to the students and followers as well.
Este documento describe cómo determinar la gravedad específica de los suelos y llenantes minerales mediante un picnómetro. Define la gravedad específica como la relación entre la masa de un volumen de sólidos y la masa del mismo volumen de agua a la misma temperatura. Explica que el método involucra calibrar el picnómetro, llenarlo con la muestra y medir las masas para calcular la gravedad específica.
Este documento presenta información sobre un colegio y la comunidad que lo rodea. Describe las características de los pueblos y ciudades, y las diferencias entre ellos. También identifica los trabajos de personas que ayudan a mantener la comunidad, como policías, jardineros y basureros. El objetivo es que los estudiantes aprendan sobre su entorno escolar y la vida en la ciudad o pueblo donde viven.
2016.09.26 studieveiledning i 2 timer web i elektroteknikk for 26.09.2016 t...Sven Åge Eriksen
2016.09.26 studieveiledning i 2 timer web i elektroteknikk for 26.09.2016 tom kap.3 - r ELEKTROTEKNIKK TOM KAPITTEL 3 SVEN ÅGE ERIKSEN FAGSKOLEN TELEMARK age, elektro, ems, eriksen, fagskolen, målebru, ohm, parallellkobling, phytagoras, seriekobling, superposisjon, sven, telemark, theorem, wheatstone, resistans, volt, ampere
Direct microscopic examination of clinical specimens can provide a presumptive diagnosis of fungal infection by revealing the presence of fungal elements. Different stains and techniques are used to visualize fungi depending on the suspected infection. KOH wet mounts are useful for superficial mycoses while GMS, H&E and fluorescent antibody stains aid in diagnosis of deep mycoses from tissue biopsies and body fluids. Proper specimen collection and rapid microscopic evaluation can help initiate appropriate antifungal treatment.
This document describes procedures for examining fecal samples to detect parasite eggs under a microscope. Several methods are discussed, including direct smear, McMaster technique, simple floatation, and sedimentation. Using these methods on samples from sheep, cattle, and dogs, several parasite eggs were observed, including Ancylostoma caninum, Toxocara canis, Fasciola eggs, Trichuris eggs, and Dipylidium caninum eggs enclosed in a capsule. The document concludes that multiple examination methods are needed to thoroughly detect parasites at different infection levels in fecal samples.
Fecal examination is commonly used to diagnose parasitic infections in animals. The process involves collecting a fresh fecal sample, preparing it using flotation or centrifugation with a flotation medium, and examining it under a microscope. Centrifugation speeds up the process by forcing heavier materials to the bottom and lighter parasite eggs to the top for easier identification. Examination of properly collected and prepared fecal samples can reveal evidence of parasitic infections and provide a diagnosis.
This ppt describes on the 5 tenets/principles, required for performing a fecal examination for parasitic eggs, larva, cyst observation required for arriving at a proper GI diagnosis.
This document discusses techniques for quantitatively estimating worm burden by calculating the number of eggs present in a stool sample. Specifically, it describes:
1) The Kato-Katz technique which involves pressing stool between a template and slide to obtain a standardized sample for egg counting under a microscope. The number of eggs observed can be used to estimate the total number of worms.
2) The Stoll technique where stool is suspended in sodium hydroxide solution and a subsample is examined. Egg counts are corrected based on stool consistency to estimate eggs per gram of formed stool.
3) The Beaver technique involves using a light meter to count eggs sandwiched between a slide and coverslip, but no details of
Microbes isolation from different environmentsMicrobiology
The document describes methods for isolating and identifying microbes from various samples. It discusses taking soil, water, food, slag and other samples and performing serial dilutions to isolate colonies. It explains incubating the samples and performing sub-culturing and biochemical tests to identify the bacterial species, including using mannitol salt agar to identify Staphylococcus aureus which turns the agar yellow. Coagulase tests are also described to differentiate S. aureus from other staphylococci.
Collection and transport of ear dischargesabeer-babeker
This document provides guidance on collecting and transporting ear discharge specimens for microbiological examination. Key points include:
1. Collect discharge on a sterile cotton swab and place in transport medium within 6 hours of collection.
2. Examine specimens microscopically with Gram stain and culture on blood agar, MacConkey agar, and thioglycollate broth. Additional media may be used depending on whether a bacterial or fungal infection is suspected.
3. Common pathogens causing ear infections include Streptococcus pneumoniae, Streptococcus pyogenes, Haemophilus influenzae, Pseudomonas aeruginosa, and fungi such as Candida species.
The document provides guidelines for virus isolation techniques using embryonated eggs, outlining various inoculation routes including allantoic, amniotic, chorioallantoic membrane and discussing advantages of different routes for isolating specific viruses. It describes the process of candling eggs to check embryo viability and mark inoculation sites prior to introducing viral samples via needle injection into selected areas. Proper egg storage, cleaning, and incubator maintenance are also covered.
This document discusses cytology of the urinary tract. It describes three methods for collecting urine specimens: voided urine, catheter specimens, and bladder washings. Voided urine is the simplest but cells may degrade over time. Catheter specimens avoid contamination but can damage cells. Bladder washings provide high cellularity and preservation through saline irrigation. Specimens can be prepared via several methods including cytocentrifugation and direct smears. Normal urinary tract cytology shows a range of superficial and deep urothelial cell morphologies depending on collection method and location in the tract.
14.anaeli and nicolle. mycobacteriophages paper.anaelishockey
Two mycobacteriophages were isolated from a soil sample collected in Gurabo, Puerto Rico. Following enrichment and plaque purification, two distinct phages (named Shockage and Zombage) were isolated based on differences in morphology and protein band patterns. Shockage had a distinct protein profile while Zombage and another initially isolated phage had nearly identical protein bands, indicating they were the same phage. Further DNA sequencing would help fully characterize these newly isolated phages and determine if they represent unique viruses. Overall, this experiment demonstrated a method for isolating new mycobacteriophages from environmental samples.
Isolation of bacteria is a significant step in diagnosing and managing bacterial infections. It involves collecting specimens, preserving and transporting them to the lab, examining samples microscopically, and using various culture and non-culture methods to isolate bacteria. Culture methods include using solid or liquid media, and automated systems, to allow bacterial colonies to grow. Non-culture methods involve molecular techniques like PCR. Proper specimen handling and use of appropriate culture conditions and media allow isolation of pathogenic bacteria to enable treatment and control of infections.
1. Proper specimen collection and transport are critical for laboratory testing.
2. Guidelines for collection include using appropriate containers and transport media, minimizing contamination, and providing complete patient information.
3. Specimens should be transported within 2 hours of collection and delivered to the laboratory promptly using biohazard labeling and packaging.
This document provides information on various laboratory investigations for sexually transmitted infections (STIs). It describes procedures for dark field microscopy, Gram staining, Giemsa staining, wet mounts, KOH wet mounts, and acetic acid testing. These tests are used to detect organisms causing STIs like syphilis, gonorrhea, chancroid, trichomoniasis, and genital HPV infections. Specimen collection sites and examination of stained slides under the microscope are discussed for accurate diagnosis of common STIs.
The document provides information about the history and types of microscopy. It discusses early microscopes developed by Hans Lippershey, Hans Janssen, and Giovanni Faber in the 1590s. Antoni van Leeuwenhoek is noted for his discoveries of micro-organisms in the 1670s using microscopy. In 1893, August Köhler developed Köhler illumination, important for modern light microscopy. Light microscopes and electron microscopes are described. Microscopic examination of samples can provide diagnostic information for conditions like malaria and other infectious diseases.
Two mycobacteriophages were isolated from soil samples collected in Gurabo, Puerto Rico using protocols from the SEA-PHAGES resource guide. The soil samples were enriched to encourage phage growth. Plaques were purified through serial streaking and enrichment to isolate individual phage clones. The two isolated phages were subjected to medium and high titer assays to increase phage particle concentration. Future work will involve sequencing the DNA of the isolated phages.
This document provides guidance on proper specimen collection, transport, and processing for the laboratory diagnosis of fungal infections. Key steps include collecting specimens in a sterile manner, transporting them to the lab within 2 hours if possible, and processing them promptly through smear preparation, culture inoculation, and incubation. Maintaining proper documentation and selecting purulent material can help maximize diagnostic sensitivity. Adhering closely to collection and handling protocols helps ensure optimal recovery of fungi in the laboratory.
Egg inoculation by Chinithung ngullie (2)Cyrus Ngullie
This presentation has been made easy to make understand to the students by emphasizing more on the visual than the words.I hope it is helpful to the students and followers as well.
Este documento describe cómo determinar la gravedad específica de los suelos y llenantes minerales mediante un picnómetro. Define la gravedad específica como la relación entre la masa de un volumen de sólidos y la masa del mismo volumen de agua a la misma temperatura. Explica que el método involucra calibrar el picnómetro, llenarlo con la muestra y medir las masas para calcular la gravedad específica.
Este documento presenta información sobre un colegio y la comunidad que lo rodea. Describe las características de los pueblos y ciudades, y las diferencias entre ellos. También identifica los trabajos de personas que ayudan a mantener la comunidad, como policías, jardineros y basureros. El objetivo es que los estudiantes aprendan sobre su entorno escolar y la vida en la ciudad o pueblo donde viven.
2016.09.26 studieveiledning i 2 timer web i elektroteknikk for 26.09.2016 t...Sven Åge Eriksen
2016.09.26 studieveiledning i 2 timer web i elektroteknikk for 26.09.2016 tom kap.3 - r ELEKTROTEKNIKK TOM KAPITTEL 3 SVEN ÅGE ERIKSEN FAGSKOLEN TELEMARK age, elektro, ems, eriksen, fagskolen, målebru, ohm, parallellkobling, phytagoras, seriekobling, superposisjon, sven, telemark, theorem, wheatstone, resistans, volt, ampere
Starbucks is an American global coffee company and coffeehouse chain based in Seattle, Washington. It has over 20,000 stores in 62 countries. Founded in 1971, Starbucks has grown to become the largest coffeehouse company in the world under the leadership of CEO Howard Schultz. Starbucks offers coffee, tea and other beverages and snacks. Its mission is to be the premier purveyor of high-quality coffee products and services. Starbucks has a strong brand but also faces threats from competitors and rising costs. It aims to maximize its market penetration through high-quality products and a relaxing atmosphere.
Jyotsana is seeking a position that utilizes her organizational skills and ability to work well with others. She has a B.E. in Computer Science Engineering from CSVTU, Bhilai with a percentage of 77.11%. Her projects include a gas agency system built in ASP.NET and a hotel management system built in C++. She is proficient in C, C++, and ASP.NET and has received scholarships and achieved high ranks in competitions.
El documento destaca la importancia de la oración cotidiana para el crecimiento espiritual y la salud espiritual. La oración es esencial para obtener sabiduría, fortaleza y guía divina. Incluso Jesús oraba frecuentemente, especialmente antes de realizar grandes tareas. La oración es necesaria en todo momento, pero especialmente cuando enfrentamos tentaciones o dificultades.
San Ezequiel Moreno nació en España en 1848 y se unió a la Orden de Agustinos Recoletos. Fue ordenado sacerdote en 1871 y trabajó como misionero en Filipinas durante 15 años. Luego fue a Colombia donde restauró la orden y revitalizó sus misiones antiguas. Más tarde se desempeñó como obispo en Colombia hasta que regresó enfermo a España en 1906, donde murió de cáncer. Fue beatificado en 1975 y canonizado en 1992, y se le atribuyen curaciones milagrosas, especialmente
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive function. Exercise causes chemical changes in the brain that may help protect against mental illness and improve symptoms for those who already suffer from conditions like depression and anxiety.
This document provides information on building resilience in students through understanding trauma and mental health issues. It discusses how trauma like sexual assault can lead to conditions like post-traumatic stress disorder (PTSD) and discusses common myths about PTSD. It provides information on understanding trauma, PTSD symptoms, and how colleges can help students by educating them on these topics and promoting help-seeking behaviors and peer support. The document advocates for creating compassionate campuses where students feel empowered and supported regarding their mental health.
The document describes using GIS techniques to enhance property tax collection in Shakarpur, East Delhi. It involves validating property data from the Delhi Spatial Data Infrastructure (DSSDI) project with records from the East Delhi Municipal Corporation (EDMC) to identify tax defaulters. The first phase involves cross-checking DSSDI spatial data on buildings and units with EDMC's tax ledger to find missing or incorrect records. The second phase entails field surveys to update property information. Analyzing the validated data identifies buildings in the DSSDI but not the ledger, and vice versa, as well as matches, helping EDMC collect taxes from defaulters more efficiently.
This document provides a guide for safer sex practices among transgender women. It begins by thanking organizations that inspired the guide's creation. It then covers topics like disclosure of transgender status and STI status, negotiating condom and dam use, tips for various sex acts like vaginal/anal sex, oral sex, rimming, toy use, and fisting. The guide emphasizes clear communication and consent, as well as using barriers like condoms and dams to reduce STI transmission risk. It aims to educate about engaging in sex acts safely.
The document discusses building distributed systems in a platform agnostic way by limiting the scope of the cluster to only data transport and semantics. This allows components to choose their own frameworks and abstraction without being locked into a particular one. It also describes using elastic partitioning to scale consumers as needed and ensure data locality by assigning partitions based on identifiers, enabling stateful processing of related events on the same worker.
Himanshu Tripathi has over 6 years of experience in business development, tendering, cost estimation, procurement, vendor management, and resource management. He holds a Bachelor's degree in Electrical Engineering and has worked in roles such as Senior Engineer - Sales, Tendering, Estimation, and Procurement at Zamil Air Conditioners India Private Limited. He is seeking a role that provides opportunities to enhance his skills and learn new skills to contribute value to an organization.
The document discusses enhancing property tax collection in Shakarpur, Delhi using GIS techniques. It involves collecting property data from the East Delhi Municipal Corporation including tax payment details. This data will be geocoded and analyzed using GIS software to calculate property taxes based on area and compare actual taxes paid. Maps will then be produced showing tax defaulters to help increase efficiency and transparency of tax collection. Ground validation will verify accuracy of property information geocoded from existing datasets.
SlideShare es un sitio web gratuito donde los usuarios pueden publicar presentaciones de PowerPoint en línea para su archivo, publicación y distribución. Los usuarios pueden crear una cuenta en SlideShare completando un breve formulario de registro que incluye su correo electrónico, nombre de usuario, contraseña y aceptación de los términos y condiciones.
Este documento presenta un cuestionario de evaluación para un programa de Ensamble y Mantenimiento de Computadores. El cuestionario contiene preguntas sobre conceptos básicos de Internet como ARPANET, TCP/IP, WWW, hipervínculos, HTTP, correo electrónico y buscadores. También incluye preguntas sobre redes como LAN, WAN, MAN y conexiones como ADSL, WiFi y satelital. Finalmente, cubre temas de seguridad como virus, antivirus y la creación de correo electrónico.
Introducción básica a Scrum, la metodología de desarrollo de software ágil. Usamos esta presentación como primer paso para inducir a nuevos miembros del equipo a nuestra manera de trabajar.
Organ culture is defined as the description and development in vitro of any organ or in the portion of organs where many tissue constituents like Parenchyma and Stroma, and there structural correlation and function are protected in culture. For what the explanted tissue narrowly look like its parent tissue in vitro.
This document discusses several culture techniques used to diagnose nematode infections based on larval morphology:
1. The Harada-Mori filter paper culture uses a filter paper strip coated with feces and placed in a test tube with water to allow hatching and development of hookworm, Strongyloides, and Trichostrongylus larvae over 10 days.
2. The Baermann technique uses a funnel with gauze and feces placed over a water reservoir, allowing rhabditiform and filariform Strongyloides and hookworm larvae to migrate through the gauze into collected fluid over 2-12 hours.
3. Charcoal culture and filter paper/slant culture
This document provides an overview of diagnostic methods for parasites. It discusses examining various clinical specimens like feces, blood, urine, sputum and biopsy material. For fecal exams, it describes macroscopic and microscopic evaluation including concentration techniques. It also covers examining blood by wet mount and stained smears to detect parasites. In addition, the document outlines methods for parasite culture, serology, molecular detection and animal inoculation for laboratory diagnosis of parasitic infections.
1. The document describes procedures for preparing slides of onion peel and human cheek cells to observe plant and animal cell structures under a microscope. Key steps include peeling onion skin, staining with safranin, mounting in glycerine, and observing cell walls, nuclei, vacuoles and cytoplasm.
2. It also provides instructions for identifying plant tissues (parenchyma, collenchyma, sclerenchyma) and animal tissues (muscle fibers, nerve cells) under the microscope from prepared slides. Observations of cell and tissue structures are recorded.
3. The objective is to learn cell and tissue structures through microscopic examination of temporary mounts and prepared slides from plant and animal samples. Structures
This document summarizes a high school student's internship researching zebrafish stem cells. The student focused on locating stem cells in the zebrafish telencephalon and brainstem through immunohistochemistry techniques. Results showed nestin-positive stem cells in the telencephalon, confirming previous findings. Additionally, an experiment conducted by the student found nestin-positive cells in the brainstem, supporting the hypothesis that stem cells there may promote central nervous system regeneration in zebrafish. Through this internship, the student gained skills in zebrafish care, dissection, sectioning, immunostaining, microscopy, and independent experimentation.
Taxonomic Collections, Preservation and Curating of InsectsKamlesh Patel
Taxonomy: Taxonomy is the science of defining and naming groups of biological organisms on the basis of shared characteristics.
The classification of organisms is according to hierarchal system or in taxonomic ranks (eg; domain, kingdom, phylum class, order, family, genus and species) based on phylogenetic relationship established by genetic analysis.
Taxonomic Collection : Biological collection are typically preserved plant or animals specimens along with specimen documentations such as labels and notations.
Dry Collection - Dry collections consist of those specimens that are preserved in a dry state.
Wet Collection - Wet collections are specimens kept in a liquid preservative to prevent their deterioration.
The document outlines procedures for testing fecal samples to identify parasite burden. It describes using a flotation solution to separate parasite eggs from fecal specimens and concentrate them at the surface for examination under a microscope. The specific steps include using a fecal flotation device to mix the solution and sample, allowing the mixture to stand, then placing a coverslip on top and examining it under a microscope at 100x magnification to identify any eggs present. Positive identification of eggs may require higher magnification. Results should be recorded in the Chameleon system.
Production method of germ free lab animalsPankaj Gaonkar
This document describes the production method of germ free lab animals. It discusses the definition of germ free animals and the equipment needed to produce them, such as isolators and transfer chambers. The key steps of the method are ensuring availability of germ free surrogate mothers, transferring pregnant donor mice to the isolator on day 17-20, performing caesarean sections in the transfer chamber to remove the uterine packages, and mixing the donor pups with the surrogate mother's litter in the isolator. Producing germ free animals allows their use to study reactions to diets, infectious disease pathogenesis, and other research.
This document provides instructions for using the Easy Culture II bacterial culture system to identify common bacteria found in milk samples from dairy cows. The system uses Bi-plates and Tri-plates that allow for the identification of gram-positive and gram-negative bacteria. Instructions are given for collecting milk samples, plating the samples on the culture plates, incubating the plates, and interpreting the results to identify bacteria and determine appropriate treatment. The goal is to help dairy farmers and veterinarians diagnose mastitis infections on farms.
The document provides guidelines for collecting and transporting various medical specimens for microbiological laboratory testing. It discusses appropriate collection, labeling, and transport methods for common specimens including blood, urine, sputum, swabs, stool, pus, and cerebrospinal fluid. Proper collection and rapid transport of adequate and correctly labeled samples are essential for successful laboratory investigations and accurate patient diagnosis and treatment.
This document outlines the protocol for anther culture, which involves growing plant cells, tissues, or organs from anthers in a sterile nutrient medium. The key steps are: 1) selecting young anthers, 2) surface sterilizing the anthers, 3) placing the anthers horizontally on nutrient medium, 4) culturing the anthers under alternating light and dark periods, 5) allowing pollen callus or plants to develop from the anthers over 3-8 weeks, and 6) transferring developed plantlets to soil for acclimatization.
This document provides an overview of the phylum Arthropoda. It discusses that arthropods make up about 85% of animal species and are found in nearly all environments. They are defined by having a jointed exoskeleton and losing motile cilia as adults. The exoskeleton allows them to be successful across habitats. Body segments are commonly fused for specialized functions. Respiration varies with habitat and vision involves simple or compound eyes. The circulatory system is open and fertilization can be internal or external.
The document discusses various techniques for obtaining pure cultures of microorganisms, including streak plating, spread plating, serial dilution, and single cell isolation. Streak plating involves streaking bacteria across nutrient agar plates using a loop to separate individual colonies. Serial dilution uses successive dilutions of a mixed culture in liquid media to isolate a predominant microorganism. Single cell isolation picks a single cell from a culture using a micromanipulator or capillary pipette and allows it to multiply, producing a pure culture. These pure culture techniques are important for laboratory study of individual microbial species.
This document provides guidance on collecting various specimen types, including blood, urine, deep specimens, feces, sputum, and genital specimens. It emphasizes the importance of collecting specimens correctly and transporting them to the laboratory promptly to ensure quality results. Key steps include using the proper collection container, obtaining informed consent, maintaining aseptic technique, and documenting and labeling the specimens accurately.
This document provides a protocol for safely collecting pooled biological samples from wild bat roosting sites in 3 sentences:
It outlines supplies needed, methods for placing tarps under tree-roosting and cave-dwelling bat colonies to collect urine and feces samples before the bats leave at dawn, and guidance for collecting and recording the samples while minimizing risks to field workers. Examples of bat feces are also provided to help with identification and differentiation from other animal waste.
Culture techniq and type of animal cell culturePankaj Nerkar
A primary culture refers to the initial culture of cells directly taken from an organism before the first subculture. A cell line refers to the propagation of cells after the first subculture. Primary cultures contain a variety of differentiated cell types and require higher cell quantities due to lower survival rates. Tissues are disaggregated into single cells using mechanical or enzymatic techniques for primary culture. Organ cultures involve culturing whole organs or tissues to preserve their structure and function in vitro. Various techniques like plasma clot, raft, and grid methods are used to culture different organ explants.
This document discusses various methods used for collecting specimens for taxonomy studies, including insects nets, aspirators, Berlese funnels, and flotation methods. It provides details on the apparatus and procedures used for each collection technique. The document also notes some of the difficulties in collection, such as political restrictions in certain countries, and aims to comprehensively sample areas before human impacts make species extinct.
This document discusses various taxonomic procedures for collecting, preserving, and identifying specimens. It describes methods for collecting insects and other organisms using various tools like nets, aspirators, Berlese funnels, and traps. It outlines the processes of sorting, relaxation, pinning, labeling, and storage of specimens. The document emphasizes the importance of proper preservation techniques like drying and use of fumigants to prevent pest damage to collections. It also discusses the roles of curators in cataloging and storing specimens and the methods taxonomists use to identify specimens by comparing them with published species descriptions.
El documento explica las alternativas al uso de animales de laboratorio, incluyendo la reducción, reemplazo y refinamiento de métodos. Describe varios tipos de alternativas como sistemas inanimados, estudios in vitro, animales y plantas poco comunes, y sistemas in vivo avanzados. También discute ejemplos específicos como pruebas químicas, biossensores, modelos basados en computadora, y cultivos de órganos.
This document discusses how purified ingredient diets are commonly used to induce metabolic syndrome in rodent models and influence their phenotypes. Purified ingredient diets allow researchers to precisely modify the nutritional composition and compare results across studies. High-fat diets ranging from 30-60% of calories from fat are often used to induce obesity in rodents. Both the level and source of fat can impact weight gain and other metabolic outcomes. Other purified ingredient diets can be formulated to induce hypertension, insulin resistance, hypertriglyceridemia, and atherosclerosis by varying the levels of fat, salt, fructose, cholesterol, and other ingredients.
Este estudio evaluó la toxicidad aguda de los extractos etanólico y acuoso de las hojas de Calea urticifolia mediante la determinación de la dosis letal media (DL50) en ratas. Los resultados mostraron que la DL50 fue mayor a 1,000 mg/kg para el extracto etanólico y mayor a 5,000 mg/kg para el extracto acuoso, sin signos de toxicidad aguda. Además, no hubo alteraciones en el peso corporal de las ratas. Los niveles séricos de urea, creatinina y transamin
This document provides an overview of various laboratory animal species used in toxicology research, including their characteristics and suitability for different types of studies. It discusses mice, rats, hamsters, guinea pigs, and rabbits, describing their physical attributes, reproductive values, housing conditions, and advantages/disadvantages for acute, chronic, carcinogenicity and developmental toxicity testing. The mouse and rat are the most widely used due to their size, costs, and physiological similarities to humans. Larger species like rabbits are also discussed.
Este documento describe los principales modelos animales experimentales de enfermedad del hígado graso. Los estudios se realizan principalmente en roedores como ratones y ratas debido a su similitud biológica con los humanos. Los modelos incluyen alteraciones genéticas que aumentan la lipogénesis hepática, como la sobreexpresión de genes que promueven la síntesis de grasas. También se describen mutaciones que dificultan la eliminación de grasas del hígado. Otras formas de inducir esteatosis hepática son median
Este documento presenta información sobre diferentes tipos de soluciones, incluyendo soluciones molar, molal, normal, porcentual y partes por millón. Explica cómo calcular y preparar cada tipo de solución, proporcionando ejemplos numéricos. El objetivo es que los estudiantes aprendan a aplicar cálculos matemáticos para elaborar soluciones de manera precisa en medicina veterinaria.
Este documento presenta la teoría y metodología para realizar tres prácticas sobre la actividad de dos enzimas hepáticas en ratas: la lactato deshidrogenasa (LDH) y la piruvato cinasa (PK). La práctica 1 determina la actividad de la LDH, sus constantes cinéticas Km y Vmax, y el tipo de inhibición por oxamato. La práctica 2 mide la actividad PK y sus propiedades cinéticas sigmoideas e inhibición por alanina y ATP. La práctica 3 calcula la constante
This document provides information and instructions for conducting a biomethodology workshop on working with hamsters. It covers objectives of the workshop including safe handling, restraint, injection techniques, blood collection, anesthesia, analgesia, and euthanasia. It then provides details on hamster biology, husbandry, identification, and techniques for injections, blood collection, and other procedures. The workshop is intended to instruct participants in humane and effective methods for working with hamsters in research.
Este documento resume las bases científicas para determinar el sufrimiento en animales no humanos. Explica que el sufrimiento implica dolor físico, enfermedades dolorosas o sensaciones emocionales desagradables. Luego detalla ocho evidencias que sugieren que los animales pueden experimentar dolor, como la presencia de receptores sensibles al dolor y estructuras cerebrales análogas. Finalmente, establece que el sufrimiento puede ocurrir cuando hay dolor físico, estrés crónico o emociones
- Se explicará el funcionamiento y uso de cada equipo.
- Se demostrará el manejo correcto de cada equipo.
- Se definirán las aplicaciones de cada equipo en el laboratorio de virología.
Alumno:
- Tomará nota de la explicación.
- Preguntará en caso de dudas.
- Participará activamente en la demostración.
Sesión I.II
Cristalería:
a).-Matraces Erlenmeyer
b).-Matraces de decantación
c).-Probetas
d).-Pipetas volumétric
Este documento describe los riesgos asociados con el uso de ultrasonidos y ofrece recomendaciones para garantizar su utilización segura. Explica brevemente el fenómeno de la cavitación y cómo los ultrasonidos se usan comúnmente en procesos químicos y de limpieza. También resume los tres principales tipos de riesgos que enfrentan los operadores al utilizar sistemas de ultrasonidos.
Chapter wise All Notes of First year Basic Civil Engineering.pptxDenish Jangid
Chapter wise All Notes of First year Basic Civil Engineering
Syllabus
Chapter-1
Introduction to objective, scope and outcome the subject
Chapter 2
Introduction: Scope and Specialization of Civil Engineering, Role of civil Engineer in Society, Impact of infrastructural development on economy of country.
Chapter 3
Surveying: Object Principles & Types of Surveying; Site Plans, Plans & Maps; Scales & Unit of different Measurements.
Linear Measurements: Instruments used. Linear Measurement by Tape, Ranging out Survey Lines and overcoming Obstructions; Measurements on sloping ground; Tape corrections, conventional symbols. Angular Measurements: Instruments used; Introduction to Compass Surveying, Bearings and Longitude & Latitude of a Line, Introduction to total station.
Levelling: Instrument used Object of levelling, Methods of levelling in brief, and Contour maps.
Chapter 4
Buildings: Selection of site for Buildings, Layout of Building Plan, Types of buildings, Plinth area, carpet area, floor space index, Introduction to building byelaws, concept of sun light & ventilation. Components of Buildings & their functions, Basic concept of R.C.C., Introduction to types of foundation
Chapter 5
Transportation: Introduction to Transportation Engineering; Traffic and Road Safety: Types and Characteristics of Various Modes of Transportation; Various Road Traffic Signs, Causes of Accidents and Road Safety Measures.
Chapter 6
Environmental Engineering: Environmental Pollution, Environmental Acts and Regulations, Functional Concepts of Ecology, Basics of Species, Biodiversity, Ecosystem, Hydrological Cycle; Chemical Cycles: Carbon, Nitrogen & Phosphorus; Energy Flow in Ecosystems.
Water Pollution: Water Quality standards, Introduction to Treatment & Disposal of Waste Water. Reuse and Saving of Water, Rain Water Harvesting. Solid Waste Management: Classification of Solid Waste, Collection, Transportation and Disposal of Solid. Recycling of Solid Waste: Energy Recovery, Sanitary Landfill, On-Site Sanitation. Air & Noise Pollution: Primary and Secondary air pollutants, Harmful effects of Air Pollution, Control of Air Pollution. . Noise Pollution Harmful Effects of noise pollution, control of noise pollution, Global warming & Climate Change, Ozone depletion, Greenhouse effect
Text Books:
1. Palancharmy, Basic Civil Engineering, McGraw Hill publishers.
2. Satheesh Gopi, Basic Civil Engineering, Pearson Publishers.
3. Ketki Rangwala Dalal, Essentials of Civil Engineering, Charotar Publishing House.
4. BCP, Surveying volume 1
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Main Java[All of the Base Concepts}.docxadhitya5119
This is part 1 of my Java Learning Journey. This Contains Custom methods, classes, constructors, packages, multithreading , try- catch block, finally block and more.
Communicating effectively and consistently with students can help them feel at ease during their learning experience and provide the instructor with a communication trail to track the course's progress. This workshop will take you through constructing an engaging course container to facilitate effective communication.
Leveraging Generative AI to Drive Nonprofit InnovationTechSoup
In this webinar, participants learned how to utilize Generative AI to streamline operations and elevate member engagement. Amazon Web Service experts provided a customer specific use cases and dived into low/no-code tools that are quick and easy to deploy through Amazon Web Service (AWS.)
This document provides an overview of wound healing, its functions, stages, mechanisms, factors affecting it, and complications.
A wound is a break in the integrity of the skin or tissues, which may be associated with disruption of the structure and function.
Healing is the body’s response to injury in an attempt to restore normal structure and functions.
Healing can occur in two ways: Regeneration and Repair
There are 4 phases of wound healing: hemostasis, inflammation, proliferation, and remodeling. This document also describes the mechanism of wound healing. Factors that affect healing include infection, uncontrolled diabetes, poor nutrition, age, anemia, the presence of foreign bodies, etc.
Complications of wound healing like infection, hyperpigmentation of scar, contractures, and keloid formation.
How to Make a Field Mandatory in Odoo 17Celine George
In Odoo, making a field required can be done through both Python code and XML views. When you set the required attribute to True in Python code, it makes the field required across all views where it's used. Conversely, when you set the required attribute in XML views, it makes the field required only in the context of that particular view.
Strategies for Effective Upskilling is a presentation by Chinwendu Peace in a Your Skill Boost Masterclass organisation by the Excellence Foundation for South Sudan on 08th and 09th June 2024 from 1 PM to 3 PM on each day.
it describes the bony anatomy including the femoral head , acetabulum, labrum . also discusses the capsule , ligaments . muscle that act on the hip joint and the range of motion are outlined. factors affecting hip joint stability and weight transmission through the joint are summarized.
Beyond Degrees - Empowering the Workforce in the Context of Skills-First.pptxEduSkills OECD
Iván Bornacelly, Policy Analyst at the OECD Centre for Skills, OECD, presents at the webinar 'Tackling job market gaps with a skills-first approach' on 12 June 2024
Gender and Mental Health - Counselling and Family Therapy Applications and In...PsychoTech Services
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!