Histopathology is the diagnosis and study of diseases of tissues under a microscope. A histopathology laboratory is specially designed to diagnose tissues as malignant or benign through microscopy and immunohistochemistry. It consists of units for biopsy collection, grossing, fixation, decalcification, tissue processing, embedding, microtomy, staining, and slide storage. Key equipment includes automated tissue processors, embedding stations, microtomes, incubators, water baths, grossing stations, cryostats, and microscopes. Storage and supplies such as containers, slides, and chemicals are also needed.

1. Histopathology Lab Organization
MLT 4 semester
Multan Institute of Health sciences

2. What is Histopathology
• Histopathology is the diagnosis and study of
diseases of the tissues, and involves examining
tissues and/or cells under a microscope.
Histopathologists are responsible for making
tissue diagnoses and helping clinicians
manage a patient’s care.

3. What is Histopathology Laboratory
• Histopathology Laboratory is specially designed
to undergo histopathological diagnosis like
studying the malignant or benign condition of
tissue
• In this Laboratory we also study morphology of
tissues
• In Immunohistochemistry section presence of
specific antigen or special molecules on tissue is
detected. We detect the presence of tumor
marker to indentify different types cancer

4. General Organization
• Biopsy Collection Unit
There are different types of Biopsies. Biopsy is
majorly done by physician or surgeon. Insure
Biopsy is properly labelled with specific
information's about patient.
• Grossing Unit for selection of required part of
tissue for further processing. Inking is also
performed to separate different parts in same
biopsy.

5. General Organization
• Fixation centre ( with a huge space to store fixed specimens)
• Decalcification unit ( Specially in case of bones to perform better
sectioning in microtomy)
• Automated Tissue Processor or Manual Tissue Processing unit in
case automated tissue processor is not available
• Embedding unit ( Embedding is mostly done with paraffin wax)
• Microtomy Unit ( In this section embedded tissues are cut into thin
sections to study the tissues under microscope most effectively)
• Staining Section ( It consist of routine staining section as well as
special staining section)
• A huge storage area for storage of Stained Slides (Most of the slides
are stored for long period of time)

6. Basic Requirements of Laboratory
Equipments
1. Automated Tissue processors( Tissue Transfer, Fluid Transfer and Microwave Tissue
Processor)

7. Basic Requirements of Laboratory
• Embedding Station

8. Basic Requirements of Laboratory
• Microtome

9. Basic Requirements of Laboratory
• Microtome Blades

10. Basic Requirements of Laboratory
• Incubator

11. Basic Requirements of Laboratory
• Water bath

12. Basic Requirements of Laboratory
• Grossing Station

13. Basic Requirements of Laboratory
• Cryostat

14. Basic Requirements of Laboratory
• Microwave

15. Basic Requirements of Laboratory
• Microscope

16. Other Requirements
• Containers

18. Storage for Stained Slides

19. Storage for Stained Slides

20. Slide Staining Dishes/ Jars

21. Microscopic Slide staining Racks

22. Glass Slides

23. Chemicals/Reagents
• Fixative
• Decalcifying Agents
• Dehydrating Agents
• Clearing Agents
• Embedding Agents
• Staining Chemicals
• Mounting Medium