- Tropical rain forests, coral reefs, and islands contain a significant amount of biodiversity that is at high risk of loss. These habitats are highly represented in 25 global biodiversity hotspots that collectively cover only 1% of the Earth's surface, located predominantly near the equator. - MacArthur and Wilson's theory of island biogeography established that the number of species on an island is related to its size and distance from mainland sources of immigrants. This theory helped explain and predict patterns of species diversity on islands. - Preserving keystone species is an effective conservation strategy because their loss can cause major ecosystem changes, significantly impacting biodiversity beyond the single species.