The final goal of the project is to develop “BioBrick” for liposome produced by means of synthetic biology, that
has a construct for disintegration embedded in its membrane. Xenobiotic packaged in a liposome is not part
of pharmacodynamics since it is biologically unavailable. Which makes liposomes interesting candidates for
universal drug delivery vectors. In our case, liposome disintegration is initiated by non-invasive sonic signal
and carried out by a construct of a sensor and an active part embedded in a membrane. Sensor part of a
construct is mechanoreceptor/mechanotransducer which activates protein representing the active part of a
construct. After activation, active part carries out the dissolution of a compartmentalization function by means
of total disintegration of vector or only membrane perforation. After an opening of a vector, previously packed
xenobiotic becomes locally available with a high concentration in locale and thus high effect and low systemic
concentration and thus smaller chance of side effect. This approach is very specific for both, time and space
factors and at the same time has a very broad area of potential biomedical applications. Vector would be, in
a hypothetical scenario of practical use in oncology, first packed with chemotherapeutics/biological drugs,
administered intravenously and then medical staff would have an option of drug activation in specific locations.
Activation is very precise and at the same time offers an option of easy switching among many different
targets, for example between dominant tumor to many potential metastasis. Since location of activation is
not tied to biomarker, but rather takes advantage of other rapidly developing medical technologies, vector
remains universally and directly applicable for any patient and for a broad spectrum of pathologies in fields of
oncology (chemotherapeutics/biological drugs and other payloads, like local immune response enhancers),
autoimmune diseases (local immune suppressors, diabetes), parasitology (malaria drugs and plasmodium
sporozoite), local pathologies (ulcer, trauma healing) . . .
The Evolution of In Situ Genetic Technologyasclepiuspdfs
In situ genetic technology was historically developed and mainly focused on detection purpose, allowing specific nucleic acid sequences to be visualized in morphologically preserved tissue sections. With the synergy of genetics and immunohistochemistry, in situ detection can correlate microscopic topological information with gene activity at the transcriptional or post-transcriptional levels in specific tissues. Furthermore, its resolution allows spatial distribution of nucleic acid products to be revealed in a heterogeneous cell population. The newest member to the franchise of in situ genetic technology is a direct-on-specimen enrichment methodology specifically for cell-free DNA liquid biopsy. Contrary to in situ detection, this in-well in situ innovation tackles the very first sample preparation step to reduce material loss, thereby improving overall sensitivity. Genomic nucleic acids purified from specimens have been proven to be time consuming and suffered from damages and losses; the evolution of in situ genetic technology offers a powerful tool for precision functional genomics, enabling cross-check between in vitro and in vivo findings. It further opens the door to ultimate genetic engineering in situ.
Nanoparticle Drug Delivery Systems for Cancer TreatmentAranca
The engineered nanoparticles are effectively used for cancer treatment due to their targeted drug delivery approach. Download the Aranca report on Technology and Patent Research for current research trends and developments.
The Evolution of In Situ Genetic Technologyasclepiuspdfs
In situ genetic technology was historically developed and mainly focused on detection purpose, allowing specific nucleic acid sequences to be visualized in morphologically preserved tissue sections. With the synergy of genetics and immunohistochemistry, in situ detection can correlate microscopic topological information with gene activity at the transcriptional or post-transcriptional levels in specific tissues. Furthermore, its resolution allows spatial distribution of nucleic acid products to be revealed in a heterogeneous cell population. The newest member to the franchise of in situ genetic technology is a direct-on-specimen enrichment methodology specifically for cell-free DNA liquid biopsy. Contrary to in situ detection, this in-well in situ innovation tackles the very first sample preparation step to reduce material loss, thereby improving overall sensitivity. Genomic nucleic acids purified from specimens have been proven to be time consuming and suffered from damages and losses; the evolution of in situ genetic technology offers a powerful tool for precision functional genomics, enabling cross-check between in vitro and in vivo findings. It further opens the door to ultimate genetic engineering in situ.
Nanoparticle Drug Delivery Systems for Cancer TreatmentAranca
The engineered nanoparticles are effectively used for cancer treatment due to their targeted drug delivery approach. Download the Aranca report on Technology and Patent Research for current research trends and developments.
Application of Microarray Technology and softcomputing in cancer BiologyCSCJournals
DNA microarray technology has emerged as a boon to the scientific community in understanding the growth and development of life as well as in widening their knowledge in exploring the genetic causes of anomalies occurring in the working of the human body. microarray technology makes biologists be capable of monitoring expression of thousands of genes in a single experiment on a small chip. Extracting useful knowledge and info from these microarray has attracted the attention of many biologists and computer scientists. Knowledge engineering has revolutionalized the way in which the medical data is being looked at. Soft computing is a branch of computer science capable of analyzing complex medical data. Advances in the area of microarray –based expression analysis have led to the promise of cancer diagnosis using new molecular based approaches. Many studies and methodologies have come up which analyszes the gene espression data by using the techniques in data mining such as feature selection, classification, clustering etc. emboiding the soft computing methods for more accuracy. This review is an attempt to look at the recent advances in cancer research with DNA microarray technology , data mining and soft computing techniques.
Nanotechnology is science, engineering, and technology conducted at the nanoscale, which is about 1 to 100 nanometers. Physicist Richard Feynman, the father of nanotechnology.
Drug delivery refers to approaches, formulations, technologies, and systems for transporting a pharmaceutical compound in the body some time based on nanoparticles as needed to safely achieve its desired therapeutic effect.
Tissue engineering is a biomedical engineering discipline that uses a combination of cells, engineering, materials methods, and suitable biochemical and physicochemical factors to restore, maintain, improve, or replace different types of biological tissues.
The field of nanotechnology was first Discovered by Professor Richard P. Feynman in 1959 (Nobel laureate in physics, 1965) [2]. Nanotechnology is the science of the small; very small and it is used for the management of substance at a small scale. At this size, molecules and atoms work in a different way, and provide a variety of unpredicted and attractive uses .
Nanotechnology essentially restructures molecules to make materials lighter, stronger, more penetrating or absorbant, among many innovative qualities. In cancer research, it offers a unique opportunity to study and interact with normal and cancer cells in real time, at the molecular and cellular scales, and during the various stages of the cancer process. For cancer researchers, a special interest lies in ligand-targeted therapeutic nanoparticles (TNP), which are expected to selectively deliver drugs and especially cytotoxic agents specifically to tumor cells and enhance intracellular drug accumulation. Targeting can be achieved by various mechanisms. For example, nanoparticles with numerous targeting ligands can provide multi-valent binding to the surface of tumor cells with high receptor density (as opposed to low receptor density on normal cells) or nanoparticle agents can enhance permeability and retention (EPR) effect to exit blood vessels in the tumor, to target surface receptors on tumor cells, and to enter tumor cells by endocytosis before releasing their drug payloads.
In this presentation we shall look at nanotechnology in drug development with a focus on anticancers and the advantages of nanoparticles as therapeutic platform technology. Approved nanotech based drugs and their clinical trials will be discussed. Two specific clinical trial case studies will be focused on along at some length with a mention of some ongoing clinical trials of nanotherapeutics. We shall also take a look at the future direction of nanotechnology based therapeutics.
El lunes 23 de octubre de 2017 celebramos una jornada en la Fundación Ramón Areces sobre Microbiota Intestinal: Implicaciones en la Salud y Enfermedad.
Application of Microarray Technology and softcomputing in cancer BiologyCSCJournals
DNA microarray technology has emerged as a boon to the scientific community in understanding the growth and development of life as well as in widening their knowledge in exploring the genetic causes of anomalies occurring in the working of the human body. microarray technology makes biologists be capable of monitoring expression of thousands of genes in a single experiment on a small chip. Extracting useful knowledge and info from these microarray has attracted the attention of many biologists and computer scientists. Knowledge engineering has revolutionalized the way in which the medical data is being looked at. Soft computing is a branch of computer science capable of analyzing complex medical data. Advances in the area of microarray –based expression analysis have led to the promise of cancer diagnosis using new molecular based approaches. Many studies and methodologies have come up which analyszes the gene espression data by using the techniques in data mining such as feature selection, classification, clustering etc. emboiding the soft computing methods for more accuracy. This review is an attempt to look at the recent advances in cancer research with DNA microarray technology , data mining and soft computing techniques.
Nanotechnology is science, engineering, and technology conducted at the nanoscale, which is about 1 to 100 nanometers. Physicist Richard Feynman, the father of nanotechnology.
Drug delivery refers to approaches, formulations, technologies, and systems for transporting a pharmaceutical compound in the body some time based on nanoparticles as needed to safely achieve its desired therapeutic effect.
Tissue engineering is a biomedical engineering discipline that uses a combination of cells, engineering, materials methods, and suitable biochemical and physicochemical factors to restore, maintain, improve, or replace different types of biological tissues.
The field of nanotechnology was first Discovered by Professor Richard P. Feynman in 1959 (Nobel laureate in physics, 1965) [2]. Nanotechnology is the science of the small; very small and it is used for the management of substance at a small scale. At this size, molecules and atoms work in a different way, and provide a variety of unpredicted and attractive uses .
Nanotechnology essentially restructures molecules to make materials lighter, stronger, more penetrating or absorbant, among many innovative qualities. In cancer research, it offers a unique opportunity to study and interact with normal and cancer cells in real time, at the molecular and cellular scales, and during the various stages of the cancer process. For cancer researchers, a special interest lies in ligand-targeted therapeutic nanoparticles (TNP), which are expected to selectively deliver drugs and especially cytotoxic agents specifically to tumor cells and enhance intracellular drug accumulation. Targeting can be achieved by various mechanisms. For example, nanoparticles with numerous targeting ligands can provide multi-valent binding to the surface of tumor cells with high receptor density (as opposed to low receptor density on normal cells) or nanoparticle agents can enhance permeability and retention (EPR) effect to exit blood vessels in the tumor, to target surface receptors on tumor cells, and to enter tumor cells by endocytosis before releasing their drug payloads.
In this presentation we shall look at nanotechnology in drug development with a focus on anticancers and the advantages of nanoparticles as therapeutic platform technology. Approved nanotech based drugs and their clinical trials will be discussed. Two specific clinical trial case studies will be focused on along at some length with a mention of some ongoing clinical trials of nanotherapeutics. We shall also take a look at the future direction of nanotechnology based therapeutics.
El lunes 23 de octubre de 2017 celebramos una jornada en la Fundación Ramón Areces sobre Microbiota Intestinal: Implicaciones en la Salud y Enfermedad.
Kits for modulation of anti-rejection therapies and early detection of neurod...Toscana Open Research
The invention consists of a nanofunctionalized device that enables the quantitative determination of a specific biomarker, the FKBP12 protein, involved in many diseases.
A General Overview of Nano Medicine-Efficacy in Therapeutic Science and Curre...Berklin
Nanotechnology’s introduction has dramatically improved a number of scientific fields, one of which is medicinal research. Nanomedicine is aimed to offer healthcare medications and chemicals a new dimension. The small size of nanoparticles, permits them to circulate in the body without interrupting oxygenation and escape filtration by both the renal and gastrointestinal networks. These are the few properties that distinguish them apart from traditional therapeutic procedures. The increased permeability and durability effect result in successful penetration inside the tumor tissues, providing cancer treatment a new lease on life. Efficient transportation pathways, on the other hand, produce genotoxicity and mutagenicity by interacting with genes that are essential for smooth functioning. As the specific interactions of nanomedicines with biological systems are still unknown, comprehending nanomedicines' toxicological effects is tough. The lack of regulatory direction in this field remains a research gap that we would want to examine in this study.
A General Overview of Nano Medicine-Efficacy in Therapeutic Science and Curre...ceijjournals
Nanotechnology’s introduction has dramatically improved a number of scientific fields, one of which is
medicinal research. Nanomedicine is aimed to offer healthcare medications and chemicals a new
dimension. The small size of nanoparticles, permits them to circulate in the body without interrupting
oxygenation and escape filtration by both the renal and gastrointestinal networks. These are the few
properties that distinguish them apart from traditional therapeutic procedures. The increased permeability
and durability effect result in successful penetration inside the tumor tissues, providing cancer treatment a
new lease on life. Efficient transportation pathways, on the other hand, produce genotoxicity and
mutagenicity by interacting with genes that are essential for smooth functioning. As the specific
interactions of nanomedicines with biological systems are still unknown, comprehending nanomedicines'
toxicological effects is tough. The lack of regulatory direction in this field remains a research gap that we
would want to examine in this study.
A well designed toxicokinetic study may involve several different strategies and depends on the scientific question to be answered. Controlled acute and repeated toxicokinetic animal studies are useful to identify a chemical's biological persistence, tissue and whole body half-life, and its potential to bioaccumulate. Toxicokinetic profiles can change with increasing exposure duration or dose. Real world environmental exposures generally occur as low level mixtures, such as from air, water, food, or tobacco products. Mixture effects may differ from individual chemical toxicokinetic profiles because of chemical interactions, synergistic, or competitive processes. For other reasons, it is equally important to characterize the toxicokinetics of individual chemicals constituents found in mixtures as information on behavior or fate of the individual chemical can help explain environmental, human, and wildlife biomonitoring studies.
Microbiology Discussion 1 While Gram staining and visualization .docxannandleola
Microbiology Discussion 1
While Gram staining and visualization under a light microscope can be powerful tools to guide a clinical microbiologist in the identification of bacteria, this process rarely, if ever, is sufficient for making a definitive diagnosis of a disease caused by bacteria. On the other hand, electron microscopy is useful for not only assisting virologists in identifying disease-causing viral agents, but may perhaps provide definitive identification of these agents. Hazelton and Gelderblom (2003)1 have made the argument that electron microscopy should be the diagnostic tool of choice in many viral outbreaks because of the rapidity and fidelity of the result.
Do you agree with the statement above or not or not and why? Explain in detail and use the evidence to support your thought.
Discuss the importance of comparing multiple images of the same virus, perhaps from different patients believed to be infected with the same agent.
1Hazelton PR, Gelderblom HR. Electron microscopy for rapid diagnosis of emerging infectious agents.Emerg Infect Dis [serial online] 2003 Mar [date cited]. Available from: URL: http://www.cdc.gov/ncidod/EID/vol9no3/02-0327.htm
Reply back to classmates: Response has to be a paragraph.
1. Yes, i do agree with Hazelton and gelderblom that the electron microscopy should be the diagnostic tool of choice. I agree with this because after reading some articles i have found that the electron microscopy is fast and realiable. When you are trying to identify a disease or viral outbreak, you are going to need something that will give you fast results that you can trust. I also think when using the electron microscopy that you should use another tool to back your findings.
2. I agree with the statement, electron microscopy has two advantages over enzyme-linked immunosorbent assay and nucleic acid amplification tests. After a simple and fast negative stain preparation, the undirected, “open view” of electron microscopy allows rapid morphologic identification and differential diagnosis of different agents contained in the specimen. Details for efficient sample collection, preparation, and particle enrichment are given. Applications of diagnostic electron microscopy in clinically or epidemiologically critical situations as well as in bioterrorist events are discussed. Electron microscopy can be applied to many body samples and can also hasten routine cell culture diagnosis. To exploit the potential of diagnostic electron microscopy fully, it should be quality controlled, applied as a frontline method, and be coordinated and run in parallel with other diagnostic techniques. This just show that Gram staining is the first step identify a bacteria, when electron microscopy will make a more result to understand where and how the bacteria was produce. I feel that electron microscopy is just a more advance way to diagnosis the reasoning on how a bacteria was caused.
3. I agree with the statement above that the electron micros ...
Combating Drug Resistance in The Intensive Care Unit (ICU)Apollo Hospitals
Drug resistance of microbes has become a major stumbling block to treating patients successfully in the ICU. There is no doubt that microbes have the capacity to mutate or acquire drug destroying enzymes, but a multitude of errors by health care providers plays a major role in facilitating the development of resistance. The maintenance of drug use discipline in closed ICUs and having a responsive microbiology department are the first steps towards prevention of microbe resistance. Having an infection control committee that is able to collect and disseminate data is the next essential step. Education of health care providers to provide uniformity of health care according to set guidelines is the culmination of this towards the goal of minimizing the development of anti microbial resistance.
INNOVATIVE MEDICINES, TECHNOLOGIES AND APPROACHES FOR IMPROVING PATIENTS' HE...Jing Zang
Despite remarkable scientific and technological achievements during the 20th century, the 21st century has already witnessed additional new and profound changes in all areas of medical science and research, including innovations and discoveries in biology, cellular biology, genomics and proteomics, pharmaceuticals, medical devices, and information technology. This review is an up-date on some of the existing therapies, drug delivery technologies, and approaches that aimed to improve patients’ health care and quality of their life.
Similar to Sonogenetic Locale Specific Activation of Universal Vectors for Xenobiotics - iGEM project proposal (20)
Gene expression control - practicum reportNejc Draganjec
Preverjanje izražanja gena za β-aktin v embriju
cebrice (izolacija celotne RNA embrija cebrice →
spektrofotometrično preverjanje uspešnosti izolacije →
reverzna transkripcija mRNA za gen β-aktin →
pomnoževanje cDNA v PCR → preverjanje na gelu)
SEMINAR - Dolgoživost, zaviranje staranja in kvaliteta življenja.Nejc Draganjec
V zadnjih nekaj letih smo bili pri\v ca vrtincu razvoja razumevanja osnovnih konceptov
biologije staranja. Med drugim so bili razviti kemi\v cni opona\k salci kalori\v cne restrikcije,
ki dokazano upo\v casni staranje pri sesalcih. Dose\v zen je bil velik napredek v kontroli-
ranju staranja pri sesalcih preko S6K1 in TOR poti. Po eni strani je bilo dokazano
upo\v casnjevanje staranja s TOR inhibitorjem rapamicinom, po drugi strani pa je nekaj
\k studij podalo rezultate, ki nasprotujejo ustaljeni resveratrol-sirtuin-kalori\v cna restrikcija-
staranje paradigmi (Anderson in Weindruch, 2013; Gems in Partridge, 2013; Martin,
2011; Selman in Withers, 2011).
Oksidativni stres ima v procesih staranja dvojno vlogo. Mo\v can oksidativni stres staranje
pospe\k suje, blag in ponavljajo\v c oksidativni stres pa \v zivljenjsko dobo organizmu podalj\k suje.
Inovativni pristop k zaviranju staranja predstavlja manipulacija endogenih celi\v cnih
obrambnih mehanizmov, kot je npr. antioksidativni odziv, preko planirane diete ali
preko farmakolo\k skih antioksidantov in/ali hormetinov. Pregled raziskav je razkril nekaj
klju\v cnih korakov in napotkov za zaviranje staranja:
1. Zmanj\k sevanje kalori\v cnega vnosa a hkrati skrb, da ne pride do podhranjenosti.
2. Prehrana s hormetini bogatimi \v zivili (sadje in zelenjava).
3. Uporaba prehrambenih dodatkov s hormetini (npr. resveratrolom).
4. Redna a zmerna fizi\v cna aktivnost in hkrati izogibanje naporni in iz\v crpavajo\v ci vadbi.
5. Izogibanje toksi\v cnim koncentracijam \k skodljivih snovi (npr. te\v zke kovine) (Anderson
in Weindruch, 2013; Gaman et al., 2011; Martin, 2011).
ELEKTRONSKA MIKROSKOPIJA MIKROGRAFIJE IN ULTRASTRUKTURENejc Draganjec
Ker je valovna dolžina elektronskega žarka 100.000-krat krajša od valovne dolžine vidne svetlobe, je teoretična ločljivost elektronske mikroskopije 0,001 nm. Toda zaradi napak magnetnih leč je dejanska maksimalna ločljivost osnovnih tehnik elektronske miroskopije okoli 0,1 nm oz 1 \r A. Praktično ločljivost pa določa tudi vrsta vzorca in njegove značilnosti. Praktično dosegljiva ločljivost bioloških vzorcev je zaradi njihovih lastnosti okoli 1 nm (Echin, 2009; Egerton, 2005; Goodhew et al., 2001; Khan, 212; Watt, 1997). Poznamo dve osnovni vrsti elektronskih mikroskopov (presevni elektronski mikroskop oz. TEM in vrstični elektronski mikroskop oz. SEM), ki se po svojih značilnostih
in principu delovanja precej razlikujeta. Konstrukcija TEM je v osnovi podobna svetlobnemu mikroskopu. Vir ``svetlobe'' nadomesti elektronska puška oz. linearni pospeševalnik iz katode in anode. Katoda poskrbi za vir elektronov in anoda za pospeševanje v smeri preparata. Snop elektronov nato potuje po koloni, v kateri moramo vzdrževati visoki vakum. Za fokusiranje in radialno pospeševanje snopa poskrbi sistem elektromagnetnih leč, ki delujejo kot kondenzor, objektiv in projektiv. Med snopom elektronov in preparatom pride do interakcij (odboj, elastično in neelastično sipanje), katerih frekvenca je odvisna od elektronske gostote preparata. Klasična priprava preparatov za TEM je postopek iz 6 korakov: fiksacija, dehidracija, vklapljanje, rezanje, prenos na nosilec in kontrastiranje s težkimi kovinami (Echin, 2009; Egerton, 2005; Goodhew et al., 2001; Khan, 212; Watt, 1997).
LOKALIZACIJA CELIČNIH STRUKTUR V BIOLOŠKIH VZORCIH Z MIKROSKOPSKIMI TEHNIKAMI...Nejc Draganjec
K izrednemu napredku mikroskopije ni prispeval le razvoj orodij, torej razvoj novih mikroskopov in izboljšava že obstoječih tehnik mikroskopiranja. Ključno je k razvoju
prispeval tudi napredek metodologije in pojav novih postopkov priprave mikroskopskih preparatov. Kadar biološki vzorec pripravljamo za mikroskopijo moramo biti še posebej pozorni na ohranjanje celične strukture in bioloških delov/molekul, ki so predmet preučevanja. Dobre metode priprav vzorcev za lokalizacijo omogočajo selektivnost
v postopku priprave, postopek mora biti natančen in občutljiv, ohranjati rekacijski produkt in omogoča enostavno in kontrastno vizualizacijo preučevane strukture. Med
pripravo ne smemo pozabiti na pozitivne in negativne kontrole (Žnidaršič, 2014).
Nekateri postopki priprave mikroskopskih preparatov so znani že zelo dolgo, takšna so predvsem nespecifična barvanja in kontrastiranje vzorcev. Mnogo tehnik, ki jih
uporabljamo danes, pa je povsem novih. Zelo pogosto se uporabljajo selektivna barvanja, dokazovanje encimske aktivnosti, imunolokalizacija, hibridizacija in situ, označevanje s fluorescenčnimi proteini (npr. GFP) (Chopra et al., 2012; Žnidaršič, 2014)...
Na vajah smo za lokalizacijo uporabili barvanja s sudan črno B (lipidi), DAPI (nukleinske kisline), hematoksilin-eozin (jedra, citoplazma in kolagen) in trikromatsko barvanje po
Massonu.
EKOPOIEZA MARSA – PRILOŽNOSTI IN OVIRE, KI JIH PREDSTAVLJA INŽENIRING NOVEGA ...Nejc Draganjec
Bistveni korak ustvarjanja novega okolja, primernega za poselitev ljudi, je načrtna vzpostavitev stabilnega ekosistema (ekopoieza). Odnosi med organizmi v stabilnem ekosistemu so kompleksni in težko predvidljivi, dodatno pa ekopoiezo otežujejo še ekstremni okoljski pogoji, ki ožijo nabor potencialnih kandidatov in s tem biološko pestrost. Ekstremna okolja na Zemlji ponujajo nekaj odgovorov in nakazujejo, da se tudi v ekstremnih razmerah spletejo trdne simbiotske naveze, ki simbiontom celo omogočajo preživetje. V nalogi je raziskan potencial lišajev za ekopoiezo v Marsovih okoljskih razmerah.
PREDSTAVITEV: EKOPOIEZA MARSA – PRILOŽNOSTI IN OVIRE, KI JIH PREDSTAVLJA INŽE...Nejc Draganjec
Bistveni korak ustvarjanja novega okolja, primernega za poselitev ljudi, je načrtna vzpostavitev stabilnega ekosistema (ekopoieza). Odnosi med organizmi v stabilnem ekosistemu so kompleksni in težko predvidljivi, dodatno pa ekopoiezo otežujejo še ekstremni okoljski pogoji, ki ožijo nabor potencialnih kandidatov in s tem biološko pestrost. Ekstremna okolja na Zemlji ponujajo nekaj odgovorov in nakazujejo, da se tudi v ekstremnih razmerah spletejo trdne simbiotske naveze, ki simbiontom celo omogočajo preživetje. V nalogi je raziskan potencial lišajev za ekopoiezo v Marsovih okoljskih razmerah.
Endosimbiontski odnosi med gostitelji nevretenčarji in fotosimbiontom so že dolgo poznani. Precej dobro so raziskani primeri gostiteljev mehkužcev (npr. Elysia chlorotica) in členonožcev. Sedaj pa se prvič pojavlja primer endosimbiontskega odnosa, pri katerem je gostitelj vretenčar. Pisani aksolotel (Ambystoma maculatum) in njegov fotosimbiont zelena alga (Oophila amblystomatis) imata tesno mutualistično zvezo, ki se prične v samem začetku embrionalnega razvoja, določa potek le-tega in se nadaljuje v larvalni stadij ter ostaja pri odrasli živali, katere fitnes je v veliki meri odvisen tudi od uspeha simbiontske zveze.
Endosimbiontski odnosi med gostitelji nevretenčarji in fotosimbiontom so že dolgo poznani. Precej dobro so raziskani primeri gostiteljev mehkužcev (npr. Elysia chlorotica) in členonožcev. Sedaj pa se prvič pojavlja primer endosimbiontskega odnosa, pri katerem je gostitelj vretenčar. Pisani aksolotel (Ambystoma maculatum) in njegov fotosimbiont zelena alga (Oophila amblystomatis) imata tesno mutualistično zvezo, ki se prične v samem začetku embrionalnega razvoja, določa potek le-tega in se nadaljuje v larvalni stadij ter ostaja pri odrasli živali, katere fitnes je v veliki meri odvisen tudi od uspeha simbiontske zveze.
Puščave - seminarska naloga pri predmetu ekologijaNejc Draganjec
Sušna območja delimo v podkategorije na podlagi več faktorjev. Upošteva se indeks precipitacija in potencialne evapotranspiracije, povprečne temperature, geografske značilnosti … Na teh faktorjih temelji groba razdelitev puščav na vroče puščave, mrzle puščave, polsuhe puščave in priobalne puščave. Puščave nudijo zelo specifične okoljske pogoje. Značilno je pomanjkanje vode, visoke ali nizke temperature, velika dnevna nihanja temperature … Biomi puščavskih ekosistemov za spopadanje z okoljskimi omejitvami koristijo številne prilagoditve, kot so pospešen razvoj, anabioza, razni sistemi intenzivnega varčevanja z vodo. Zaradi specifičnih zahtev je biološka diverziteta v ekosistemih puščav praviloma nižja, kot v večini drugih ekosistemov. Zato intenzivno širjenje puščav (dezertifikacija) predstavlja precejšnji izziv v trenutnem, hitro spreminjajočem se podnebju.
Puščave- predstavitev seminarske naloge pri predmetu ekologijaNejc Draganjec
Sušna območja delimo v podkategorije na podlagi več faktorjev. Upošteva se indeks precipitacija in potencialne evapotranspiracije, povprečne temperature, geografske značilnosti … Na teh faktorjih temelji groba razdelitev puščav na vroče puščave, mrzle puščave, polsuhe puščave in priobalne puščave. Puščave nudijo zelo specifične okoljske pogoje. Značilno je pomanjkanje vode, visoke ali nizke temperature, velika dnevna nihanja temperature … Biomi puščavskih ekosistemov za spopadanje z okoljskimi omejitvami koristijo številne prilagoditve, kot so pospešen razvoj, anabioza, razni sistemi intenzivnega varčevanja z vodo. Zaradi specifičnih zahtev je biološka diverziteta v ekosistemih puščav praviloma nižja, kot v večini drugih ekosistemov. Zato intenzivno širjenje puščav (dezertifikacija) predstavlja precejšnji izziv v trenutnem, hitro spreminjajočem se podnebju.
One zoom - biološka podatkovna zbirka in predvsem orodje za vizualizacijoNejc Draganjec
One zoom je orodje za vizualizacijo po principu fraktalne periodične geometrije. Skupaj z orodjem pridejo tudi odlične biološke podatkovne zbirke filogenije sesalcev, dvoživk ...
Evolucija raka - vpliv naravne selekcije na evolucijo onkoloških obolenjNejc Draganjec
Rak je bolezen genoma. Klasični model karcinogeneze opisuje večkratno sosledno širjenje klonov, ki ga vodi nabiranje genetskih sprememb (mutacij) in pozitivna selekcija okolja v katerem se tumor pojavi. Toda z evolucijskimi metodami lahko raziskujemo onkološka obolenja tudi iz vidika makro in populacijske evolucije. Naravna selekcija je poskrbela za kompleksne in prepletene mehanizme regulacije in popravljanja genoma in abnormalne celične delitve. Toda, pogosto pride tudi do antagonistične koevolucije in posledičnih pleotropičnih učinkov genov, ki v določenem obdobju osebka njegov fitnes dvigujejo a imajo hkrati v kasnejših obdobjih življenja onkogeno vlogo. Na tak način mehanizmi Darwinove evolucije v populaciji ne izkoreninjajo bolezni kot je rak, ampak fiksirajo onkogene in gensko podlago za takšno delovanje senescence na sploh.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Sonogenetic Locale Specific Activation of Universal Vectors for Xenobiotics - iGEM project proposal
1. N E I T S (U R), Vol. I, No. 2, 2-6, 2016
A project proposal – iGEM 2016
Sonogenetic Locale Specific Activation of
Universal Vectors for Xenobiotics.
Nejc Draganjec1*, Roman Jerala2
Abstract
The final goal of the project is to develop “BioBrick” for liposome produced by means of synthetic biology, that
has a construct for disintegration embedded in its membrane. Xenobiotic packaged in a liposome is not part
of pharmacodynamics since it is biologically unavailable. Which makes liposomes interesting candidates for
universal drug delivery vectors. In our case, liposome disintegration is initiated by non-invasive sonic signal
and carried out by a construct of a sensor and an active part embedded in a membrane. Sensor part of a
construct is mechanoreceptor/mechanotransducer which activates protein representing the active part of a
construct. After activation, active part carries out the dissolution of a compartmentalization function by means
of total disintegration of vector or only membrane perforation. After an opening of a vector, previously packed
xenobiotic becomes locally available with a high concentration in locale and thus high effect and low systemic
concentration and thus smaller chance of side effect. This approach is very specific for both, time and space
factors and at the same time has a very broad area of potential biomedical applications. Vector would be, in
a hypothetical scenario of practical use in oncology, first packed with chemotherapeutics/biological drugs,
administered intravenously and then medical staff would have an option of drug activation in specific locations.
Activation is very precise and at the same time offers an option of easy switching among many different
targets, for example between dominant tumor to many potential metastasis. Since location of activation is
not tied to biomarker, but rather takes advantage of other rapidly developing medical technologies, vector
remains universally and directly applicable for any patient and for a broad spectrum of pathologies in fields of
oncology (chemotherapeutics/biological drugs and other payloads, like local immune response enhancers),
autoimmune diseases (local immune suppressors, diabetes), parasitology (malaria drugs and plasmodium
sporozoite), local pathologies (ulcer, trauma healing) . . .
Keywords
sonogenetics — pharmacodynamics — oncology — drug delivery — xenobiotics — autoimmune diseases —
igem
1Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia
2Department of Biotechnology, National Institute of Chemistry, 1000 Ljubljana, Slovenia
*Corresponding author: nejc@draganjec.me
Contents
Introduction and motivation 2
Methods and project pipeline 3
iGEM and proof of concept . . . . . . . . . . . . . . . . . 3
Practical application and publication . . . . . . . . . . 4
References 4
Introduction and motivation
Already established approaches with pharmaceuticals [1,
2], optogenetics [3, 4] and sonogenetics [5, 6, 7, 8, 9, 10]
all have their own advantages for basic research and indus-
try and they compliment each other. But no method pub-
lished up until now has managed to avoid general applicative
problems of synthetic biology and genetic engineering in
medicine:
• In West, there is still a taboo on the direct engineer-
ing of the human genetic pool. But there is general
acceptance of products made by synthetic biology,
like almost all modern insulin supply [11, 12, 13].
• Since we already have an effective treatment, for an
instance injection of insulin, taking risks with new
methods of GE, cell therapy, implantation ...is un-
founded for [4].
• Very often solution offered is not actually a solution
for pathology but rather for a consequence of it. In
such case, a patient is offered an impossible choice
between being dependent on old vs. being dependent
on new technology. For example, switch from de-
pendency on insulin injection to dependency of an
external signal that activates insulin production in
micro-encapsulated implanted cells. [8, 9, 4].
For organizations, media attention is often a very real
part of the decision to participate in iGEM. Which makes
choosing the right topic at least as important as having the
right team and support environment [14, 15]. Media cov-
erage, which follows egocentric and topical attention of
public, is focused mostly on applicative value of research
2. Sonogenetic Locale Specific Activation of Universal Vectors for Xenobiotics. — 3/5
Figure 1. a) VesiColi BioBricks. b) Luciferase as a payload for liposome. c) Sequences of different sensor and active parts
of membrane construct. d) Transformation of a host organism in case of “in vivo” liposome production. e) Liposome
production in a bioreactor. f) Extraction of the product. g) There are many alternatives if it turns out that quality of
VesiColi BioBricks is not sufficient. We can use some other well-established liposome producing host, or turn away from
“in vivo” liposome production altogether and go with, for example, hydration of thin layer method. This is critical point and
a decision made at this step affects almost all other steps too. h) If we go with “in vivo” and we have time, resources and
interest, we can check the success of transformation at this point. Most of commercially available kits allow for easy
checkpoints. i) Product testing. Testing for payload, in the previous example for luciferase, is easily done by maceration
and mixing with enzyme substrate (luciferin) and then comparing the signal with control without maceration. Testing of
membrane construct is dependent on its components, but we could use immunolabeling and microscopy, patch-clamp for
ion channels, hybrid systems ...
from fields that catch public attention in certain period the
most (usually fields like oncology, infectology, metabolic
disorders, gerontology ...) [16, 17, 18]. So it is in no
surprise that anecdotal experiences scientist have with jour-
nalist’s questions like “ ...how does this cure cancer ...”
translate in only 0,001–0,005 % media coverage of scien-
tific research outside of medicine and health general topics
[18].
The fact that a lot of pharmaceuticals that are commonly
administered only works as expected for 25–60 % of pa-
tients [19] and that this resulted in 142.000 deaths only in
the year 2013 [20] was acknowledged as one of the biggest
current issues in medicine. Many methods were already
researched and proposed to improve on pharmacokinetics
and to assure more targeted delivery [21, 22, 23]. An issue
with current approaches is that specificity negates flexibil-
ity which makes medical treatment time consuming and
costly. This is furthermore complicated by pathologies that
combine homogeneity between pathology and healthy soma
and heterogeneity inside pathology tissue. Perfect examples
are tumors where systemic therapy, because of similarity
with healthy soma, carries devastating side effects. And
at the same time, cancerous cells go through cell clonal
evolution and extensive differentiation which makes precise
and dependable specific targeting very difficult [24].
Methods and project pipeline
As experienced mentors can surely testify, there is never an
excess of time for a big project like iGEM. That is why I
propose we split path to a final product in 2 stages – proof
of concept for iGEM and applicative test for a publication.
iGEM and proof of concept (Figure 1 and 2)
In the spirit of iGEM, where cooperation is the norm, I
think it makes sense if we start with a good idea developed
Figure 2. a) Isolated vectors are transferred to medium
with an enzyme substrate. Luciferin for example that was
given before. b) We sonicate samples and measure the light
signal. c) Between different membrane constructs we
measure and compare stability (unspecific permeability for
payload or even full disintegration of liposome), dynamics
(dissolution of the compartmentalization function after
activation) and interval of effective signal
amplitude/frequency (energy must not be too high so that
tissue “in vivo” is not damaged and not too low so that we
don’t have unspecific activations and poor stability).
by VesiColi iGEM NTNU 2013 team (bronze price) and
upgrade it to excellent. In case “bioBricks” from VesiColi
team are not of sufficient quality, we still have many other
methods of liposome production on our disposal (other
3. Sonogenetic Locale Specific Activation of Universal Vectors for Xenobiotics. — 4/5
Figure 3. a) We prepare 3 combinations of vector and cell line – the first vector with membrane construct and payload,
second with payload but without construct and third as a negative control without construct and payload. b) We start with
cell culture and monitor stability and potential toxicity of vector. c) We sonicate cultures and analyze differences between
active, passive and negative control vectors. The difference between active and passive gives the efficiency of membrane
construct and negative control represents baseline normalization and test for potential general toxicity of liposome in
culture. d) If we stay in the field of oncology, we can continue tests on some of many oncology model lines of rodents. But
since this method of delivery is universal, we also have the option to switch payload and focus on a mirage of other
possible pathologies. For example, we could deliver local immune suppressors to locale of pancreas and follow the
prognosis of diabetes 1 in diabetic rodent line.
liposome producing hosts, hydration of thin layer ...).
Our main contribution to synthetic biology would be
“bioBrick” construct for controlled opening of the liposome
by means of a sonic signal. For mechanoreceptor, we have
many options, but I would recommend we look at MscL,
MscS and MCA protein families since they do not have a
homologue in animals. The absence of homology lowers
chance of unspecific effects in the final practical application
as a biomedical tool. Native presence in liposome producing
host (or perhaps just membrane construct if we decide on
other liposome production method) also makes synthesis
part of the project easier since we only have to modify and
not design cell pathways “de novo”. The active part of a
construct is porin (example [25]) or split enzyme which
gets activated after sensor part of constructs receives sonic
signal. Activation could be direct by on enzyme attached
ligand/receptor (as in [26]) or indirect through cell signaling
cascade. Main work in this segment of the project would
be building and testing different combinations of sensor
and active part of construct and testing which combination
offers the right combination of precision, dynamics and
reliability.
Furthermore, I propose, we make sure that in testing
period liposomes carry a payload which offers an easy as-
sessment of approach viability in next steps. As an example,
we could pack liposomes with luciferase during production.
After successful transformation of the host organism (in
a case of “in vivo” production) for test payload and mem-
brane construct we can start with production in a bioreactor.
Isolation and purification of product out of reactor media is
dependent on steps before, but as an example, we could po-
tentially use some well-established detergent method ([27]).
Isolated vectors can then be transferred to test medium
with added substrate for the enzyme of choice in a pay-
load. In the beforehand example that would be luciferin.
Then we sonicate test dishes and we record light signal as
a result of the disintegration of compartmentalization and
mixing of enzyme/substrate with optical methods (for ex-
ample microscopy). In case statistically significant signal in
the locale of sonic signal focus we have proof of working
concept. We can also check for time-dependent stability of
vectors by the same principle but without sonic signal.
Practical application and publication (Figure 3)
We follow up the basic proof of concept with a test of per-
formance in a more complex system of cell culture. The
best platform would be one of immortal human cell lines to
which we add a vector with payload of appropriate cytotox-
icity. After cells in culture adhere in place we can sonicate
and then we compare the formation of plaques between test
and control dishes. After success in cell cultures, we can
continue tests on some of many oncology model lines of
rodents.
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