Sodis effectiveness in water purification revised
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This document summarizes a student research project that tested the effectiveness of solar disinfection (SODIS) for water purification. Water samples were collected from two brooks and exposed to sunlight for varying durations, both with and without aluminum foil wrapping. Testing with Colilert found that control samples wrapped in foil showed high bacterial levels, while unwrapped samples exposed to sunlight for the full experiment time showed some disinfection. The results suggest SODIS requires direct and constant UV exposure to effectively disinfect water, which was limited on the day of the experiment. Future studies are planned to test SODIS under different weather conditions and times of day.
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This document summarizes a study on the effectiveness of solar disinfection (SODIS) for water purification. Water samples were collected from two brooks and exposed to UV light for varying durations, both with and without aluminum foil wrapping. While the control samples wrapped in foil remained highly contaminated, samples exposed to UV light without wrapping for the full six hours showed some disinfection of bacteria. However, cloudy weather on the day of the experiment limited direct sunlight exposure to only two hours, likely preventing full disinfection via the SODIS method. Future experiments are planned to test additional variables over different daylight conditions.
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- 1. Solar Disinfection(SODIS) Effectiveness in Water Purification Adriana Rivera Aguayo Ivan Garcia Motta May 2011 BIOL 4997 Prof. Eneida Díaz
- 2. Introduction One of the most important basic necessities of human beings: potable water. Some are not able to have this service due to various variables: Limited access Poverty Natural disasters
- 3. Introduction Water that is not properly processed can have high concentrations of bacteria such as E.coli and coliforms. Escherichia Coli (E.coli), bacteria responsible for different gastrointestinal health problems.
- 4. Solar Disinfection (SODIS) A transparent PET bottle is cleaned with soap. Then, the bottle is filled with water and placed in full sunlight for at least 6 hours. The water has then been disinfected and can be drunk.
- 5. Colilert Colilert uses the patented Defined Substrate Technology® (DST®) to simultaneously detect total coliforms and E. coli. As coliforms grow in Colilert, they use β-galactosidase to metabolize ONPG and change it from colorless to yellow. E. coli use β-glucuronidase to metabolize MUG and create fluorescence. Since most non-coliforms do not have these enzymes, they are unable to grow and interfere.
- 6. Hypothesis UV rays are the cause for disinfection in the SODIS method, not the temperature. In order to see a decrease in the colonies of E.coli.
- 7. Methodology Phase I (Sample collection) Label 14 bottles with the numbers and a letter identifying each brook. (A) brook with less contamination; (B) brook with more contamination. Collect water samples from two different brooks, localization provided by the professor. In 14 PET bottles, fill ¼ of the bottle with the brook’s water to clean it. Then collect the sample by filling completely the bottle. Samples have to be put in ice at 4°C.
- 8. Methodology Phase II (Sample Incubation and Colilert) Four samples from each brook (1A-4A; 1B-4B), have to be covered in aluminum foil. Only sample 1A and 1B have to be kept in 4°C. The other three samples have to be exposed to UV rays. The other six samples will be exposed to UV rays without the aluminum foil. Every two hours, two samples( one with aluminum foil and one without it) will be removed. The environment temperature will be measured, also the waters temperature and written down. And put them in 4°C.
- 9. Methodology After the six hours, you have to let all the samples lower their temperatures to 37°C. Then put them in 100mL Erlenmeyer flask, add a Colilert package and transfer it to a quantitray and seal it. Incubate the samples in 35°C for 24 hours. After this period count the positive results.
- 10. Results Bothcontrolshadextremelyhighconcentrations of total coliforms and E. coli. Allthebottleswrapped in aluminumfoilshowed a significantamount of contamination and a posible repopulation of bacteria. Unwrappedbottlesthatwereleftforthelength of thewholeexperimentshowedsomedisinfection.
- 11. Results
- 12. Possible Reasons for no Disinfection SODIS requires direct and constant UV rays in order to disinfect. Weather conditions on the day of the experiment only allowed for approximately two hours of direct sunlight.
- 13. Futures Plans Implement this research with more variables and at different times of the day.