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biosecurity built on science
Development of a female Qfly lure
Andrew Finlay, Summerfruit Australia
&
Paul Cunningham
Research Leader, AgriBio, DEDJTR, VIC.
biosecurity built on science
Female Qfly Lures
Feeding
Egg laying
Bactrocera tryoni
biosecurity built on science
Phase 1: Proof of concept
Blends containing fruit ripening
volatiles attract female Qfly
Proof of concept 
11V
• analytical chemistry
• blend formulation
• electrophysiology
• cage trials
A SYNTHETIC FRUIT ODOUR TO
ATTRACT MATED FEMALE FLIES
biosecurity built on science
Phase 2: Formulations & Field studies
Finding the right mix
biosecurity built on science
Phase 2: Formulations & Field studies
Finding the right mix
FEMALE PHEROMONES
Day 12 mated
Day 3 virgin
Deterrent?
• Solvent extraction
• Rectal gland extracts
• Insect emissions
biosecurity built on science
Phase 2: Formulation & Field studies
Finding the right mix
YEASTS Cryptococcus flavescens
Aureobasidium pullulans
Rhodotorula sp (2)
Hannaella sp (3)
Pichia fermentans
Sydowia eucalypti
C. flavescens
biosecurity built on science
Phase 2: Formulation & Field studies
Finding the right mix
Deterrents
• Females avoid larval infested fruit
• Acetoin emissions
biosecurity built on science
Phase 2: New technologies
Applying calcium imaging of the antennal
lobe to insect lure development
Volatile-evoked responses
in the Qfly antennal lobe
Qfly brain
Improved methods for
attractant formulation
across insect pests
• blend synergy
• volatile detection
• valence
biosecurity built on science
Fieldwork 2016/17
Field trials:
2. Mated vs unmated capture
rates
• feeding vs egg laying lures
1. Trial lure formulations
• x 4 formulations
• New dispensers
Tatura (VIC)
Stanthorpe (QLD)
biosecurity built on science
Benefits of female lure research
Commercial fruit growers / consultants
 Monitoring / surveillance
 Reducing Qfly damage
 Lowering within / between season populations
 Reducing urban Qfly populations
 Improved market access
 Reduction in production losses
Sterile Insect Technique
 Assessment of success
 Lowering populations
Scientists
 Olfactory research
 Developing new technologies for pest management
biosecurity built on science
Thank you
Project Team
Jane Royer (QDAF)
Pawel Sadowski (QUT/CARF)
Mikael Carlsson (Stockholm University, AL imaging)
Tony Clarke (QUT)
Tommaso Villa (female pheromones)
Owen Webb (acetoin emissions)
Alex Piper (yeast analysis)
Summerfruit Australia
Andrew Finlay (Chairman)
Rowan Berecry

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Session 7: Development of a female Q-fly lure

  • 1. biosecurity built on science Development of a female Qfly lure Andrew Finlay, Summerfruit Australia & Paul Cunningham Research Leader, AgriBio, DEDJTR, VIC.
  • 2. biosecurity built on science Female Qfly Lures Feeding Egg laying Bactrocera tryoni
  • 3. biosecurity built on science Phase 1: Proof of concept Blends containing fruit ripening volatiles attract female Qfly Proof of concept  11V • analytical chemistry • blend formulation • electrophysiology • cage trials A SYNTHETIC FRUIT ODOUR TO ATTRACT MATED FEMALE FLIES
  • 4. biosecurity built on science Phase 2: Formulations & Field studies Finding the right mix
  • 5. biosecurity built on science Phase 2: Formulations & Field studies Finding the right mix FEMALE PHEROMONES Day 12 mated Day 3 virgin Deterrent? • Solvent extraction • Rectal gland extracts • Insect emissions
  • 6. biosecurity built on science Phase 2: Formulation & Field studies Finding the right mix YEASTS Cryptococcus flavescens Aureobasidium pullulans Rhodotorula sp (2) Hannaella sp (3) Pichia fermentans Sydowia eucalypti C. flavescens
  • 7. biosecurity built on science Phase 2: Formulation & Field studies Finding the right mix Deterrents • Females avoid larval infested fruit • Acetoin emissions
  • 8. biosecurity built on science Phase 2: New technologies Applying calcium imaging of the antennal lobe to insect lure development Volatile-evoked responses in the Qfly antennal lobe Qfly brain Improved methods for attractant formulation across insect pests • blend synergy • volatile detection • valence
  • 9. biosecurity built on science Fieldwork 2016/17 Field trials: 2. Mated vs unmated capture rates • feeding vs egg laying lures 1. Trial lure formulations • x 4 formulations • New dispensers Tatura (VIC) Stanthorpe (QLD)
  • 10. biosecurity built on science Benefits of female lure research Commercial fruit growers / consultants  Monitoring / surveillance  Reducing Qfly damage  Lowering within / between season populations  Reducing urban Qfly populations  Improved market access  Reduction in production losses Sterile Insect Technique  Assessment of success  Lowering populations Scientists  Olfactory research  Developing new technologies for pest management
  • 11. biosecurity built on science Thank you Project Team Jane Royer (QDAF) Pawel Sadowski (QUT/CARF) Mikael Carlsson (Stockholm University, AL imaging) Tony Clarke (QUT) Tommaso Villa (female pheromones) Owen Webb (acetoin emissions) Alex Piper (yeast analysis) Summerfruit Australia Andrew Finlay (Chairman) Rowan Berecry