This document discusses several topics related to HIV and immune response. It mentions that IL-27 inhibits HIV replication in CD4+ T cells and plays a role in signaling and immune response. It also discusses that YB-1 protein plays roles in cell processes and may help support HIV replication. Several methods are discussed like PCR, western blotting, and electrophoresis that can help understand HIV invasion and replication mechanisms and potentially lead to new treatments or prevention.

1. Paula Andrea Olier Arango
Estudiante Medicina- Tercer
Semestre
2019-2

2. IL-27
Y-BOX
T-CD4 cells
It is produced by APC; plays an
important role in the first stages of
signaling to TH1 contributing in a
paracrine way to the control of the
response to IL-12
Also called helpers. They help other cells of
the immune system to come up the answer
against the antigen. They also allow the
differentiation of B cells and participate in
the macrophage's activation
Is a member of a large family of
proteins; is involved in several cellular
processes including proliferation,
differentiation, and stress response.

3. The HIV is a lentivirus, a type
of retrovirus
Causes the syndrome of
acquired immunodeficiency,
illness that was described
originally in 1981.
Is transmitted by sexual
contact, parenteral exposure
to contaminated bood or
from the mother to the son
during the perinatal period.
It doesn´t have a cure yet.
At present it is not counted
yet with a vaccine against the
HIV.
HIV uses the CD4 molecule as a
receptor, and it is expressed in
magropahages and T-lymphocytes

4. “Interleukin-27 (IL-27) is known as an
anti-viral cytokine that inhibits HIV,
HCV and other viruses. We have
previously demonstrated that, IL-27
post-treatment after HIV infection
inhibits viral replication in primary
CD4+T cells”

5. Es un método que permite la medición
de características físicas y químicas de
células como el tamaño, forma,
complejidad y, cualquier componente
celular o función
Hacer pasar células en suspensión
alineadas por delante de un haz
luminoso.
Se miró la expresión de
CD4, CXCR4 y CD3 en
los Linfocitos T-CD4
¿Qué es?¿Qué es?
¿cómo??
¿Para qué?

6. Examen usado para detectar la
reacción de un antígeno con
anticuerpo en sangre.
El anticuerpo o antígeno objeto de
estudio se vincula a una enzima
específica.
Se miró la replicación
viral a partir de los
niveles de p24
¿Qué es?¿Qué es?
¿cómo??
¿Para qué?
El antígeno p24 es una
proteína vírica, sus niveles en
sangre están elevados al inicio
de la infección por el VIH

7. Reacción en cadena de la polimerasa
con transcriptasa inversa, es utilizada
para la amplificación de un fragmento
de RNA
A través de la síntesis previa de cDNA
utilizando una transcriptasa inversa
seguidos de varios ciclos de PCR
convencional
Se midieron los niveles de expresión
génica de la YB-1
¿Qué es?¿Qué es?
¿cómo??
¿Para qué?

8. Es una técnica analítica usada en
biología molecular para identificar
proteínas específicas en una mezcla
compleja de proteínas
Se desnaturalizan las proteínas y se
usa una corriente eléctrica para
extraerlas a través de un gel,
proceso denominado electroforesis.
Para mirar qué tanta afinidad tiene
una proteína por un antígeno
¿Qué es?¿Qué es?
¿cómo??
¿Para qué?

13. Author Contribution
Weydert C, van
Heertum B, Dirix L, De
Houwer S, De Wit F, Mast
J, et al.
A recent study using T-cell lines reported that YB-1
helps support HIV replication in cell line
Kohno K, Izumi H,
Uchiumi T, Ashizuka M,
Kuwano M.
It is known that YB-1 is a multifunctional protein
that plays important roles in transcriptional
and translational regulation, DNA repair, drug
resistance, and stress responses to extracellular
signals
Matsumoto K, Wolffe AP
The YB-1 protein consists of 324 amino acids
composed of three domains: the N
terminal Alanine/proline-rich domain (amino acids
1–54), Cold Shock Domain (CSD) (amino acids 55–
128), and C-terminal domain (amino acids 129–
324)

14. ✓Methods such as western blotting, PCR,
electrophoresis, among others, help us to
understand better the different mechanisms of
invasion and replication of microorganisms, such
as HIV; and with this open the possibility of trying
new cures or prevention methods.
✓Interleukins play a fundamental role in the body's
immunological response and being able to study
its actions more thoroughly in the face of various
stimuli allows us to move forward in the treatment
of many diseases, including HIV