Antibiotic resistance and molecular characterization of enteroaggregative Escherichia coli isolated from patients with diarrhea in the Eastern Province of Saudi Arabia

1. S E M I N A R - M O L E C U L A R B I O L O G Y
Third semester
Medicine UPB 2020
Juan José Vargas Herrera
María Camila Trejos Herrera

2. INTRODUCTION
ENTEROAGGREGATIVE ESCHERICHIA COLI
Is responsible for causing cases of persistent
diarrhea in children and adults throughout
the world
It is a gram-negative bacteria
Grow at 37 ° C

3. ANTIBIOTIC
RESISTANCE
The surveillance of antibiotic resistance is very
important in bacterial isolates due to continuous
application of antibiotics in treatment of
enteropathogenic infection
EAEC revealed resistance to tetracycline,
ampicillin, nalidixic acid, trimethoprim
sulfamethoxazole, ciprofloxacin, streptomycin,
noroxin, and piperacillin.
Cephalosporins and fluoroquinolones are the
most common used.

4. "To investigate presence of Enteroaggregative Escherichia coli (EAEC) in
patients suffering with diarrhea by targeting the pCVD432 (pAA) gene using
PCR"
OBJECTIVE

5. CEPAS DE MUESTRAS FECALES
63 Cepas de E.Coli
52 cepas de adultos
11 cepas de niños
MÉTODOS
VITEK 2
PCR
ERIC-PCR
Mayo 2013 - Julio 2014

6. pCVD432
PCR
“Técnica cuyo objetivo es la
amplificación directa de un
gen o un fragmento de
ADN o indirecta de un RNA”
FUNDAMENTO
La PCR se utilizó para examinar
todas las cepas de E. coli en busca de
marcadores de genes de virulencia
de EAEC.
USO EN EL ESTUDIO
aggR, aap, astA, y aaf/II

7. ERIC-PCR
USO EN EL ESTUDIO
Analizar todas las cepas mediante
consenso intergénico repetitivo de
enterobacterias (ERIC-PCR) para
rastrear la relación clonal
"Basada en la amplificación de
las secuencias de ADN a través de
la PCR, usando cebadores
específicos para cada tipo de
secuencia"

8. PRUEBA DE SUSCEPTIBILIDAD
A LOS ANTIBIÓTICOS
"Empleado para determinar la sensibilidad de un agente
microbiano frente a un antibiótico o quimioterápico"
Se usó para 20 agentes antimicrobianos y los antibióticos
probados

9. FIGURA #1
"Perfiles ERIC-PCR y patrones de resistencia
a antibióticos de 63 cepas DEC aisladas de
pacientes con diarrea en KFHU"
GENÉTICAMENTE IGUALES
GENÉTICAMENTE DIFERENTES
Non -EAEC6, EAEC15 y EAEC75

10. FIGURA #2
"Perfiles representativos de ERIC"

11. TABLA #2
"Caracterización de factores de virulencia
de cepas de EAEC aisladas de pacientes
con diarrea"

12. La combinación más prevalente del perfil del factor de
virulencia en las cepas de la EAEC fue
TABLA #3
"Prevalencia de genes de virulencia entre cepas de EAEC aisladas de pacientes con diarrea"
aap, astA
aap, aggR, astA

13. DISCUSSION
Kong et al., 2015
AUTHOR
Globally, EAEC shows an alarming
increase in resistance to different
ranges of antibiotics
AUTHOR´S EXHIBITION AGREE: YES O NO

14. Huang et al., (2006)
EAEC is responsible of causing
persistent and chronic diarrhea in
different group of populations in
developing and industrialized countries.
AUTHOR AUTHOR´S EXHIBITION AGREE: YES O NO
This suggests that strains of EAEC with
a “stacked-brick” AA lack AAF and
might have other adhesion
mechanisms
Boisen et al. (2008)
DISCUSSION

15. CONCLUSIONS Eric PCR is an important test
because gather similarities
between different strains of E
coli
E coli is the most studied bacterial
species and used to investigate DNA
replication, genetic code, gene
expression and protein synthesis
The antibiotic susceptibility testing is
important because in this article allow
identify the resistance of different
strains of e coli versus medicines

16. J U A N J O S É V A R G A S
H E R R E R A

17. M A R Í A C A M I L A T R E J O S H E R R E A