Seminario Biología Molecular
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This document analyzes antibiotic susceptibility and clonal distribution of Staphylococcus aureus bacteria found in pediatric patients at a children's hospital in Colombia over an 8-year period. Researchers used PCR, restriction enzymes, and sequencing to detect genes in the bacteria and determine resistance. They found that some samples contained genes indicating resistance to methicillin. The overall goal was to evaluate changes in S. aureus strains and antibiotic susceptibility trends over time in this pediatric patient population.
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Seminario Biología Molecular
This study analyzed genome and methylome variations in the bacterial pathogen Helicobacter pylori during experimental infection in humans. Researchers used single-molecule real-time sequencing to analyze H. pylori isolates obtained from human volunteers involved in a vaccine trial. The isolates came from individuals infected with a strain known as BCM-300, which has virulence factors associated with disease. The results showed changes in key virulence genes in the bacteria, suggesting an ability to adapt during infection, even in vaccinated individuals. However, the small number of volunteers limited conclusions about differences between vaccinated and unvaccinated groups.
Multidrug Resistance Pattern of Staphylococcus Aureus Isolates in Maiduguri ...
This study investigated the multidrug resistance patterns of Staphylococcus aureus isolates in Maiduguri, Nigeria. Samples were collected from hospitals and tested for S. aureus, which was isolated from 38.8% of samples, primarily from wound swabs. Antibiotic sensitivity testing found high resistance to ciprofloxacin (64.3%), ampicillin (59.5%), and chloramphenicol (54.8%). Multiple antibiotic resistance was observed in over 92% of isolates. The multiple antibiotic resistance index was highest at 0.6 (23.8%) and 0.5 (19.0%), indicating resistance to multiple drugs. The results demonstrate high multidrug resistance in S. aureus isolates from the
Multidrug Resistance Pattern of Staphylococcus Aureus Isolates in Maiduguri M...
Multi drug-resistant (MDR) isolates of Staphylococcus aureus are on rise and are becoming a challenge for timely and appropriate treatment. The present study was carried out with an objective to isolate Staphylococcus aureus from clinical samples and determine their sensitivity. Out of 110 samples collected, 44 were shown to contained S. aureus. The isolates were subjected to antibiotic sensitivity tests using 10 different and commonly used antibiotics by modified Kirby- Bauer disc diffusion technique. Out of the total isolates (42) tested, only 7.1% were susceptible to all the antibiotics. Multiple resistance was eminent in over 92% with highest occurrence in 4.8% where the entire antibiotics were resisted. Multiple antibiotic resistance indixes (MAR index) indicated that 0.6 index occurred most (23.8%) followed by 0.5 (19.0%). On the other hand, 0.1 and 0.8 indexes were the lowest with 0.0% and 1.0% occurrence respectively. Ciprofloxacin was resisted by most of the organisms (64.3%) while amoxicillin (64.3%) and streptomycin (61.9%) were most efficacious. With over 90% isolate having MAR index ≥ 0.2, the multiple drug resistance by the S. aureus is quite alarming and might suggest inappropriate antibiotic usage by the sampled population. Therefore, the need to strategize the nature of antibiotic treatment against S. aureus and massive campaign on indiscriminate antibiotic use is urgent.
10 jurnal yang bertemakan Antibiotic resistance in bacteria di Scopus beserta...
This document summarizes 10 journal articles on antibiotic resistance in bacteria from the Scopus database. It provides information on the objective, methods, parameters, conclusions, and quartile ranking for each article. The articles cover topics like evaluation of antibiotic resistance in E. coli from fermented foods in India, molecular mechanisms of resistance in E. coli and K. pneumoniae, detection of resistance in E. coli from salads in India, emergence of colistin resistance in A. baumannii in India, characterization of UPEC isolates from India, global dissemination of the mcr-1 gene, trends in antimicrobial resistance of E. coli in South India over time, detection of high-level colistin resistance mediated
Asnmnt 1
This document summarizes the selection and characterization of recombinant clones from a Mycobacterium leprae expression library screened with sera from household contacts of leprosy patients. Twelve recombinant clones were selected that reacted with antibodies in the contact sera. These antigens recognized by contact sera differed from those recognized by monoclonal antibodies and were not identical to known M. leprae antigens. Two groups of antigens were identified: one recognized by all contact sera, and one recognized by only some contact sera. These antigens may be relevant to early immune responses during M. leprae infection.
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Seminario Biología Molecular
- 1. Valeria Ortiz Tabares Tercer semestre Universidad Pontificia Bolivariana 2022
- 2. This is a gram positive bacteria How its spread - Physical contact - Sharing personal stuff - Cough and sneezes It causes: - Food poisoning - Bacteremia - Septic arthritis Staphylococcus aureus
- 3. Antifungal resistance: it occurs when viruses, bacteria and fungi fight off the medicine effect. ANTIBIOTIC SUSCEPTIBILITY The staphylococcus aureus is resistant to methicillin
- 4. OBJECTIVE The overall objective is to evaluate clonal Distribution and Antibiotic Susceptibility of Staphylococcus aureus from Pediatric Patients: 8-Year Trends in a Children’s Hospital in Colombia.
- 5. METHODS PCR It´s a polymerase chain reaction , it finds the DNA and RNA of a pathogen ➔ They used a multiplex PCR to detect nuc, mecA and lukS/lukF-PV genes ➔ They amplified 7 housekeeping genes such as arcC, gmk, aroE, glpF, pta, tpi and yqil RESTRICTION OF ENZYMES The restriction enzyme prevents replication of the bacterial DNA by cutting it into many pieces. ➔ They used HhaI (restriction enzyme) that amplified product for the gmk gene
- 6. METHODS SEQUENCING It is used to determine de orden of bases within DNA. The fragments of DNA were subjected to sequencing. ANTIBIOTIC SUSCEPTIBILITY It detects an inducible resistance. The D-zone test for inducible clindamycin resistance was performed for all the isolates resistant to erythromycin to detect the inducible MLSB (iMLSB) phenotype.
- 7. RESULTS ATCC 29213, SA-6, SA-7- it only has the nuc gene
- 8. RESULTS
- 9. DISCUSSION AUTHOR STATEMENT AGREE / DISAGREE Chambers H F et al “Epidemic clones of MRSA have disseminated glo- bally with some of them reaching high prevalence in different geographical areas.” ✅ Melles DC et al “Analysis of the population structure of pathogenic S. aureus clones has determined that certain clones are more virulent than others.” ✅ Choo EJ “The emergence of community- associated MRSA (CA-MRSA) strains in healthcare settings has changed the hospital epidemiology of MRSA in the last few years.” ✅
- 10. CONCLUSION Through molecular biology we can analice how some type of bacteria and viruses can identify their resistance to certain type of antibiotics. Using different studies all followed up by molecular biology it can identify different type of antibiotics that a the Staphylococcus Aureus is resistant to, this information can be used later to treat infections caused by this gram positive bacteria.