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EVALUATION OF FOOT-AND-MOUTH DISEASE O SKR VACCINE
1. ACADEMIC RESEARCH POSTER TEMPLATE
Subtitle for Academic Research Poster (48x36 inches)
Your names and the names of the people who contributed to this presentation
Introduction
Since FMD outbreaks in 2010-2011,
nationwide vaccination program has been
launched in Republic of Korea. A commercially
available vaccine containing FMDV O1 Manisa
(Middle-East South Asia lineage) strain had
been successfully used for mandatory
vaccination until a new outbreak of FMD (Mya-
98 lineage) was occurred in Jincheon County in
2014. After 2014, serotype O antigen changes
in FMD vaccine was inevitable to reinforce the
efficacy of the vaccine. In this regard, several
commercial FMD vaccines were tested to
determine a proper vaccine strain for the usage
of a prophylactic measure, including a
commercial O SKR (O SKR7/10) vaccine
originated from FMD virus O/SEA/Mya-98
lineages that occurred in 2010 in Republic of
Korea. In this study, we assessed the efficacy of
the O SKR 7/10 vaccine in pigs using in vivo
challenge test with FMDV O Jincheon (O-JC)
strain. In addition, a field efficacy trial of these
vaccine candidates was undertaken to estimate
the possibility of field application.
S. Jung1, J. Choi1, S. Lee1, H-H. Kim1, J-H. Park1, and J. Kim*1
EXPERIMENTAL EVALUATION OF FOOT-AND-MOUTH DISEASE O
SKR VACCINE: PROTECTIVE EFFICACY AND SEROLOGICAL
PERFORMANCE IN PIGS
1Center for FMD Vaccine Research, Animal and Plant Quarantine Agency, Gimcheon, Korea
Materials and methods
Twenty FMD-free, specific pathogen-free (SPF)
Yucatan pigs (8 weeks old) were used in the
experiment to evaluate the efficacy of the O
SKR 7/10 vaccine. Two groups of 5 pigs each
(groups A and B) were vaccinated with one
dose (2 ml) at 8 weeks old and challenged at 14
and 28 days postvaccination (dpv), respectively.
The other group of 5 pigs (group C) was
vaccinated twice with one dose
each at 8 and 12 weeks old and challenged 14
days after the boost vaccination. One group of
3 pigs (group D) was unvaccinated and
Challenged.
Fig. 1 Changes in the clinical
scores and FMDV RNA levels in
pigs immunized with the O SKR
7/10 vaccine after a contact
FMDV challenge by contact
with donor pigs inoculated
with the O-JC strain. The FMDV
RNA levels in the sera and
nasal swabs were measured by
qRT-PCR from 0 to 14 days
after the challenge. # number
at the top left of each graph
indicates the pig ID. (A) 14 dpv,
(B) 28 dpv, (C) 42 (prime) dpv,
(D) unvaccinated. Error bars
represent the standard errors
of the mean (SEMs).
Discussion
According to vaccine matching results of the
FMD WRL, antigenic relationship between O-JC
and O SKR 7/10 was very high (r1 value ≥ 0.92).
In vivo efficacy studies of the vaccine
demonstrated that the O SKR vaccine can
confer complete protection in SPF pigs against
homologous challenge during the experiment
period (Fig. 1). The serologic test results
indicated that the vaccine developed vigorous
antibody responses at 7 dpv(Fig.2).
On the other hand, in the field trial, the O SKR
vaccine did not elicit a satisfactory immunity in
the vaccinated pig; a rapid decrease of antibody
level in pig after the first vaccination (Fig. 3).
This result may be due to the inhibitory
antibody response caused by the pre-existing
FMD antibody level (SP-antibody level at 0 dpv)
from the maternal transmission..
Reference
[1] Brehm DJ et al. 2008. Vaccine. 26:1691-7.
[2] Park JN et al. 2014. Vaccine. 32:1882-9.
[3] Kim T et al. 2019. Vaccine. 37:1702-9.
Efficacy tests :
Field trials :
To evaluate the serological performance of
the O SKR 7/10 vaccine in the field, a total of
150 crossbred pigs raised on 3 different
conventional pig farms were vaccinated once at
8 weeks old or twice at 8 and 12 weeks old.
Serology :
Pig sera was collected at regular intervals to
measure antibody levels. The level of structural
protein (SP) antibody in pig serum was
determined by serotype O SP-ELISA kit. Virus
neutralizing (VN) antibody titer against
O/Andong/SKR/2010 (O-AD) or the O-JC strain
was measured according to previous method
described elsewhere
Results
Fig. 2 ELISA and VNT antibody responses in pigs
administered the O SKR 7/10 vaccine. Along with
unvaccinated pigs, which served as the control
group, all vaccinated pigs were challenged with the
O-JC strain at 14 or 28 dpv in the single vaccination
groups, and at 42 days after prime vaccination in
the double vaccination group. (A) SP ELISA PI levels
to type O antigen, (B) NSP ELISA PI levels, (C) VNT
titers to the O-JC strain, and (D) VNT titers to the
O-AD strain. (A) 14 dpv, (B) 28 dpv, (C) 42 (prime)
dpv, (D) unvaccinated. Error bars represent SEMs.
Fig. 3 ELISA (A) and VNT (B) antibody responses in
the single-vaccinated groups and double-
vaccinated groups in field trials with the O SKR
7/10 vaccine. Each of 3 conventional pig farms had
a single-vaccinated group that was immunized
once at 8 weeks old and a double-vaccinated
group that was immunized twice at 8 and 12
weeks old. However, the IC farm had mistakenly
administered an additional vaccination in both the
immunized groups after 16 weeks old. The NIAS
had only one group that was vaccinated twice at 4-
week intervals. The NIAS farm belongs to the NIAS
in South Korea. Black arrows indicate booster
vaccinations. White arrows indicate accidental
vaccination. (A) SP ELISA titers to type O antigen,
(B) VNT titer to the O-AD strain. Error bars
represent the SEMs. wpv: weeks postvaccination.
*p < 0.05, **p < 0.01, ***p < 0.001.
Taken together our results, it can be
concluded that the immunogenicity or antigen
payload of O SKR vaccine seems to be
somewhat lacking for the field because it does
not overcome the interference effect of
maternally derived FMD antibodies.
![ACADEMIC RESEARCH POSTER TEMPLATE
Subtitle for Academic Research Poster (48x36 inches)
Your names and the names of the people who contributed to this presentation
Introduction
Since FMD outbreaks in 2010-2011,
nationwide vaccination program has been
launched in Republic of Korea. A commercially
available vaccine containing FMDV O1 Manisa
(Middle-East South Asia lineage) strain had
been successfully used for mandatory
vaccination until a new outbreak of FMD (Mya-
98 lineage) was occurred in Jincheon County in
2014. After 2014, serotype O antigen changes
in FMD vaccine was inevitable to reinforce the
efficacy of the vaccine. In this regard, several
commercial FMD vaccines were tested to
determine a proper vaccine strain for the usage
of a prophylactic measure, including a
commercial O SKR (O SKR7/10) vaccine
originated from FMD virus O/SEA/Mya-98
lineages that occurred in 2010 in Republic of
Korea. In this study, we assessed the efficacy of
the O SKR 7/10 vaccine in pigs using in vivo
challenge test with FMDV O Jincheon (O-JC)
strain. In addition, a field efficacy trial of these
vaccine candidates was undertaken to estimate
the possibility of field application.
S. Jung1, J. Choi1, S. Lee1, H-H. Kim1, J-H. Park1, and J. Kim*1
EXPERIMENTAL EVALUATION OF FOOT-AND-MOUTH DISEASE O
SKR VACCINE: PROTECTIVE EFFICACY AND SEROLOGICAL
PERFORMANCE IN PIGS
1Center for FMD Vaccine Research, Animal and Plant Quarantine Agency, Gimcheon, Korea
Materials and methods
Twenty FMD-free, specific pathogen-free (SPF)
Yucatan pigs (8 weeks old) were used in the
experiment to evaluate the efficacy of the O
SKR 7/10 vaccine. Two groups of 5 pigs each
(groups A and B) were vaccinated with one
dose (2 ml) at 8 weeks old and challenged at 14
and 28 days postvaccination (dpv), respectively.
The other group of 5 pigs (group C) was
vaccinated twice with one dose
each at 8 and 12 weeks old and challenged 14
days after the boost vaccination. One group of
3 pigs (group D) was unvaccinated and
Challenged.
Fig. 1 Changes in the clinical
scores and FMDV RNA levels in
pigs immunized with the O SKR
7/10 vaccine after a contact
FMDV challenge by contact
with donor pigs inoculated
with the O-JC strain. The FMDV
RNA levels in the sera and
nasal swabs were measured by
qRT-PCR from 0 to 14 days
after the challenge. # number
at the top left of each graph
indicates the pig ID. (A) 14 dpv,
(B) 28 dpv, (C) 42 (prime) dpv,
(D) unvaccinated. Error bars
represent the standard errors
of the mean (SEMs).
Discussion
According to vaccine matching results of the
FMD WRL, antigenic relationship between O-JC
and O SKR 7/10 was very high (r1 value ≥ 0.92).
In vivo efficacy studies of the vaccine
demonstrated that the O SKR vaccine can
confer complete protection in SPF pigs against
homologous challenge during the experiment
period (Fig. 1). The serologic test results
indicated that the vaccine developed vigorous
antibody responses at 7 dpv(Fig.2).
On the other hand, in the field trial, the O SKR
vaccine did not elicit a satisfactory immunity in
the vaccinated pig; a rapid decrease of antibody
level in pig after the first vaccination (Fig. 3).
This result may be due to the inhibitory
antibody response caused by the pre-existing
FMD antibody level (SP-antibody level at 0 dpv)
from the maternal transmission..
Reference
[1] Brehm DJ et al. 2008. Vaccine. 26:1691-7.
[2] Park JN et al. 2014. Vaccine. 32:1882-9.
[3] Kim T et al. 2019. Vaccine. 37:1702-9.
Efficacy tests :
Field trials :
To evaluate the serological performance of
the O SKR 7/10 vaccine in the field, a total of
150 crossbred pigs raised on 3 different
conventional pig farms were vaccinated once at
8 weeks old or twice at 8 and 12 weeks old.
Serology :
Pig sera was collected at regular intervals to
measure antibody levels. The level of structural
protein (SP) antibody in pig serum was
determined by serotype O SP-ELISA kit. Virus
neutralizing (VN) antibody titer against
O/Andong/SKR/2010 (O-AD) or the O-JC strain
was measured according to previous method
described elsewhere
Results
Fig. 2 ELISA and VNT antibody responses in pigs
administered the O SKR 7/10 vaccine. Along with
unvaccinated pigs, which served as the control
group, all vaccinated pigs were challenged with the
O-JC strain at 14 or 28 dpv in the single vaccination
groups, and at 42 days after prime vaccination in
the double vaccination group. (A) SP ELISA PI levels
to type O antigen, (B) NSP ELISA PI levels, (C) VNT
titers to the O-JC strain, and (D) VNT titers to the
O-AD strain. (A) 14 dpv, (B) 28 dpv, (C) 42 (prime)
dpv, (D) unvaccinated. Error bars represent SEMs.
Fig. 3 ELISA (A) and VNT (B) antibody responses in
the single-vaccinated groups and double-
vaccinated groups in field trials with the O SKR
7/10 vaccine. Each of 3 conventional pig farms had
a single-vaccinated group that was immunized
once at 8 weeks old and a double-vaccinated
group that was immunized twice at 8 and 12
weeks old. However, the IC farm had mistakenly
administered an additional vaccination in both the
immunized groups after 16 weeks old. The NIAS
had only one group that was vaccinated twice at 4-
week intervals. The NIAS farm belongs to the NIAS
in South Korea. Black arrows indicate booster
vaccinations. White arrows indicate accidental
vaccination. (A) SP ELISA titers to type O antigen,
(B) VNT titer to the O-AD strain. Error bars
represent the SEMs. wpv: weeks postvaccination.
*p < 0.05, **p < 0.01, ***p < 0.001.
Taken together our results, it can be
concluded that the immunogenicity or antigen
payload of O SKR vaccine seems to be
somewhat lacking for the field because it does
not overcome the interference effect of
maternally derived FMD antibodies.](https://image.slidesharecdn.com/s-201211130846/85/experimental-evaluation-of-footandmouth-disease-o-skr-vaccine-protective-efficacy-and-serological-performance-in-pigs-1-320.jpg)
