This presentation covers methods that can be utilized to detect microbes in waste water

1. DETECTION OF MICROBES IN
WASTEWATER
NDOU L.F 20006782
NDOU T 21002581
MALULEKE M 21007995

2. INTRODUCTION
• Wastewater contains a diverse array of
microorganisms, including bacteria, viruses, fungi,
and parasites.
• These microorganisms find their way into treated
wastewater effluents which poses a risk to the
environment and public health.
• The detection, identification, and enumeration using
microbiological analysis is crucial.
• Physiochemical analysis of treated wastewater
effluents gives insights on their water quality.
• Our main focus will be based on bacteria and virus
such mycobacterium tuberculosis, Escherichia coli,
Klebssiella and Human Adenovirus.

3. OBJECTIVES
To detect/investigate the presence of bacteria and viruses in
wastewater in the Vhembe district of the Limpopo province,
South Africa.
To determine the presence/ prevalence of bacteria and viruses
using PCR.
To determine water quality through the analysis of
physicochemical parameters.

4. Sample collection
Sample
preparation
Membrane
filtration
Centrifugation
Culture based
method
Physicochemical
analysis
Physicochemical
e.g pH,
temperature, COD,
TDS and DO

5. SAMPLE COLLECTION AND
PREPARATION
• Samples will be collected from each WWTP's influent and
effluent, upstream and downstream points of the receiving
waterbodies.
• Collected samples will be stored in ice immediately after
sampling and then transported to the university of Venda general
laboratory pending analysis using microbiological methods.
• Physicochemical analysis of the collected will give insights on
the quality of river water.
• The presence of bacteria wastewater effluent will be established
through standard culture methods.
• Membrane filtration will be done trap microorganisms in the 0.22
μm membrane filter paper and centrifugation will be done to
separate substances based on their size, shape and density.

6. EXTRACTION AND PCR
DNA/RNA
extraction
PCR (polymerase
chain reaction )
PCR is molecular
biology technique
used to amplify
DNA sequence
There are few types
of PCR such as
conventinal PCR
and real time PCR

7. MICROBIOLOGICAL
ANALYSIS
Standard culturing
methods will be
conducted for the
identification of
bacteria of
interest.
The streaking
technique will be
performed on
selective media.
Agar plates will be
incubated
overnight.
The following day,
agar plates will be
viewed for
bacterial growth.

8. DATA ANALYSIS
• Data analysis will be captured in excel and all graphs
and statistics will be generated using Microsoft excel.

9. TOOLS AND KITS