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RNA
(RNA STRUCTURE, RNA SYNTHESIS, MRNA PROCESSING IN
EUKARYOTES)
Cherry Anne C. Mendoza BS BIO-II
RNA SYNTHESIS
MRNA PROCESSING
IN EUKARYOTES
Processing of mRNA begins with transcription
Soon after, RNA Polymerase begins transcription
A Methylated Cap is added to 5’ end. Transcription continues.
Following completion, RNA Polymerase releases the cap strand of pre-mRNA
Specific nucleotide sequence in the mRNA are bound by cleavage factors.
The 3’ end of the mRNA next move into the correct configuration of cleavage.
Stabilizing Factors are then added to the complex.
Poly A Polymerase now binds to the mRNA and cleaves the 3’end.
The complex begins to dissociate and the cleaved 3’ end quickly degrades.
Poly A Polymerase now synthesizes the polyadenylated tail by adding adenine
residues to the cleavage site.
Additional proteins then bind to the tail increasing the rate at which it grows.
When the tail reaches its full length the Poly A Polymerase is signaled to stop.
Adding residue and the polyadenylation process is completed. The processed
mRNA is now ready to undergo splicing in preparation for translation.
RNA

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