The document discusses RNA synthesis and mRNA processing in eukaryotes. It describes that mRNA processing begins with transcription by RNA polymerase, which adds a 5' methylated cap. Specific sequences in the pre-mRNA are then bound by cleavage factors, moving the 3' end into the correct configuration for cleavage. Poly A polymerase then binds and cleaves the 3' end, synthesizing an adenine tail, with additional proteins binding to increase tail growth until it reaches full length. The processed mRNA is then ready for splicing before translation.

1. RNA
(RNA STRUCTURE, RNA SYNTHESIS, MRNA PROCESSING IN
EUKARYOTES)
Cherry Anne C. Mendoza BS BIO-II

17. RNA SYNTHESIS

31. MRNA PROCESSING
IN EUKARYOTES

37. Processing of mRNA begins with transcription

38. Soon after, RNA Polymerase begins transcription

39. A Methylated Cap is added to 5’ end. Transcription continues.

40. Following completion, RNA Polymerase releases the cap strand of pre-mRNA

41. Specific nucleotide sequence in the mRNA are bound by cleavage factors.

42. The 3’ end of the mRNA next move into the correct configuration of cleavage.

43. Stabilizing Factors are then added to the complex.

44. Poly A Polymerase now binds to the mRNA and cleaves the 3’end.

45. The complex begins to dissociate and the cleaved 3’ end quickly degrades.

46. Poly A Polymerase now synthesizes the polyadenylated tail by adding adenine
residues to the cleavage site.

47. Additional proteins then bind to the tail increasing the rate at which it grows.

48. When the tail reaches its full length the Poly A Polymerase is signaled to stop.

49. Adding residue and the polyadenylation process is completed. The processed
mRNA is now ready to undergo splicing in preparation for translation.